Digital Commons Network^™

Xanthine Oxidase Catalyzed Oxidation Of Acetaldehyde Substrate And Carboxylate Inhibition, Patrick A. Goodman

All Master's Theses

In recent years, interest in an enzyme which catalyzes the oxidation of a variety of aldehydes and purines has been growing steadily. In fact, this enzyme commonly known as xanthine oxidase, has been implicated by some (1) in the regulation of nucleic acid synthesis and cellular growth - an important consideration in the study of caner. Unfortunately, detailed investigations directly pertaining to biological aspects of this enzyme have as yet been somewhat limited. Nevertheless, the amount of information concerning the nature of the enzyme and its catalytic effects upon different classes of substrates, through far from complete, is indeed significant.

Cadmium In Kittitas County Soils, Don G. Muller

All Master's Theses

This paper deals with a survey of soil cadmium concentrations in Kittitas County, Washington, using atomic absorption spectrophotometry as the analytical method. The purpose of the survey was to establish, in an area relatively free from cadmium contamination, background levels for a wide variety of soil conditions. The results showed cadmium concentrations ranging from amounts below the detection limit in remote areas to 2.58ppm in cultivated, inhabited areas, with an overall average concentration of 0.12 ppm cadmium

A Crystalline Fragment Of The Double Helix: The Structure Of The Dinucleoside Phosphate Guanylyl-3',5'-Cytidine, Roberta Ogilvie Day, Nadrian C. Seeman, John M. Rosenberg, Alexander Rich

Department of Chemistry: Faculty Publications

The sodium silt of guanylyl-3',5'-cytidine crystallizes in a monoclinic unit cell with one molecule in the asymmetric unit. Each molecule is related to another molecule by a 2-fold rotation axis which results in the formation of an antiparallel; right-handed .double helix with complementary hydrogen bonding between the guanine and cytosine residues. The crystal is heavily hydrated with 36 water molecules in the unit cell. The geometry of this crystalline double helix is. very similar to those which have been derived from studies of fiber x-ray diffraction patterns of double-stranded RNA; even though the latter do not yield data at atomic …

Final Report On Interrelationships Between Chemical, Physical And Biological Conditions Of The Waters Of Las Vegas Bay Of Lake Mead, James E. Deacon, Richard W. Tew

Publications (WR)

This program was a status study of the interaction between Las Vegas Wash, an enriched stream, and Las Vegas Bay, a wedge shaped arm of one of the world's deeper reservoirs. The program centered primarily on identification and counting of planktonic algae from several points in Las Vegas Bay. Additional work on nutrient enrichment of water samples was conducted to aid in interpretation of algal distribution related to nutrient input. Examination of a variety of physical, chemical, and biological parameters, both at many surface points in the bay, as well as in vertical profile, was also accomplished and further aided …

The Isolation And Purification Of A Fluorescent Compound From Pseudomonas, Thomas M. Davey

Masters Theses

Fluorescence in certain land snails has been found to arise from a bacteria carried by the snails. The bacteria, a genus of Pseudomonas, produce a pigment complex in iron-deficient media. The pigment complex consists of both fluorescent and non-fluorescent pigments. One of the fluorescent compounds was isolated and purified by paper chromatography using several different solvent systems.

Absorption and fluorescent spectra of the compound at a number of pH values were measured and the changes with pH of both the intensity and the color of the fluorescence were observed. The purified compound was ninhydrin negative but after acid hydrolysis …

Some Aspects Of Mercury Accumulation By The Purple Shore Crab Hemigrapsus Nudus Dana (Crustacea: Decapoda), Ralph Gene Swanson

University of the Pacific Theses and Dissertations

The present study has attempted to investigate some of the more basic questions concerning the toxicity and accumulation of mercury in the purple shore crab, Hemigrapsus nudus. This rocky intertidal denizen is found in abundance along the entire Pacific coast of the United States from Sitka, Alaska, to the Gulf of California where it dominates the mid-tide pool region. Local specimens were collected just north of the Pacific Marine Stations, Dillon Beach, Marin County, California. The animal is hearty and can be kept in a state of good health for long periods of time in the laboratory with minimum care …

The Atomic Absorption Determination Of Ultra-Trace Tellurium In Rocks Utilizing High Sensitivity Sampling Systems, Richard D. Beaty

Doctoral Dissertations

"The sampling boat and the graphite furnace have been shown to possess the required sensitivity to detect tellurium at ultra-trace levels, in a variety of sample types, by atomic absorption. In the sampling boat approach, tellurium in sample solutions is chemically separated and concentrated by extraction into methyl isobutyl ketone before measurement. For samples exhibiting extraction interferences or excessively high background absorption, a preliminary separation of tellurium by coprecipitation with selenium is described. Using this technique, tellurium can be quantitatively detected down to 5 nanograms and linear response is observed to 100 nanograms. Relative standard deviations of better than 7% …

The Effect Of Organic Solvents On The Sensitivity Of Rare Earths Atomic Absorption, Keith A. Geiken

Dissertations and Theses @ UNI

Atomic absorption of the rare earths erbium, europium, and dysprosium was studied in 10% organic solvent-water systems, water miscible solvent-water systems, and in 100% solvent solutions of the metal acetylacetonates. Sensitivity was found to depend on the solvent system used and instrument parameters. All solvent systems used enhanced absorption, with (1) higher number of carbon atoms, (2) tertiary and secondary alcohols, and (3) high boiling point solvents producing the largest increase in absorption. No significant viscosity relationships existed in the 10% solvent-water systems for either miscible or partially soluble solvents; however, viscosity might have been a factor in absorption in …

Ua66/7/2 Newsletter, Wku Chemistry

WKU Administration Documents

Newsletter created by and about the WKU Chemistry department.

Acidities In Ethanol/Water Solvents: The Pka Of Hf, Raymond M. Merrill

Chemistry and Chemical Biology ETDs

The acidity of hydrofluoric acid in mixed ethanol/ water solvent systems was studied by determining the pKa of HF in a number of different ethanol concentrations. The effect of changing temperature on the acidity of HF in these media was also examined. The pKa's were determined using a lanthanum fluoride membrane Fluoride Specific Ion Electrode and a glass pH electrode versus the same reference electrode. The method eliminates the need to determine liquid junction potentials. By determining the pKa for HF in each ethanol/water mixture, at a number of different temperatures, thermodynamic parameters, such as free energy, enthalpy, entropy, and …

On Improved Wkb (Uniform Asymptotic) Quantum Conditions, Dunham Corrections, The Langer Modification, And Rkr Potentials, Russell T. Pack

Faculty Publications

An improvement to the WKB quantum condition for the rotation-vibration levels of diatomic molecules, known empirically to give RKR potentials of increased accuracy and to account for the Dunham correction Y00, is derived using uniform asymptotic methods. The role of the Langer transformation in this problem is also discussed.

Principles Of Control Theory As Applied To A Thermostat, Joseph Peter Stidham

Master's Theses

The recent development of solid state electronics has opened new possibilities in instrumentation design. One development has been the availability of compact, low cost, high gain, D. C. amplifiers which can be used in the design and construction of sensitive laboratory instruments (11). Using these amplifiers a thermostat capable of precise control can be constructed and its performance compared with the performance expected by an analysis of the closed loop control equations.

14th Annual Rocky Mountain Spectroscopy Conference

Rocky Mountain Conference on Magnetic Resonance

Program and abstracts from the 14th annual meeting of the Rocky Mountain Spectroscopy Conference, sponsored by the Rocky Mountain Section of the Society for Applied Spectroscopy. Held in Denver, Colorado, July 31 - August 1, 1972.

Synthesis Of Derivatives Of Pyrido [3,4-D]Pyridazines, Diane (D'Ottavio) Martin

Chemistry and Chemical Biology ETDs

The current interest in the biological activity of certain 1 ,4-disubstituted pyrido[2 ,3-d]pyridazines prompted the investigation of various derivatives of the isomeric pyrido[3,4-d]pyridazine ring system. Thus, 1,4 ­ dichloro[3,4-d]pyridazine was treated with thiourea and the intermediate thiouronium salt hydrolyzed to give pyrido[3, 4-d]pyridazine-1,4(2H,3H)-dithione which was subsequently alkylated to give 1,4-bis(alkylthio)pyrido­[3,4-d]pyridazines. On the other hand, 1,4-bis(arylthio)­pyrido[3,4-d]pyridazines were prepared by the reaction of 1,4-dichloropyrido[3,4-d]pyridazine with various substituted thiophenols. Furthermore, treatment of 1,4-dichloropyrido[3,4-d]­pyridazine with a variety of substituted anilines gave the corresponding 1,4-disubstituted aniline derivatives, whereas reaction with alkylamines afforded only the 1(4)-chloro-4(1)-alkylaminopyrido[3,4-d]pyridazines. When 1-chloropyrido[3,4-d]pyridazine-4(3H)-one or 4-chloropyrido[3,4-d]pyridazine-1(2H)-one was treated with hydrazine, the corresponding …

Enzymatic Synthesis Of Oligoribonucleotides Of Defined Base Sequence, Mark J. Rowe

Theses and Dissertations

The synthetic reaction of ribonuclease T_1 has been studied as to its potential for the synthesis of oligoribonucleotides of defined base sequence. Conditions have been determined for maximum synthesis with respect to percentage incorporation of both guanosine 2',3'-cyclic phosphate (donor), and cytidine (acceptor). With regard to maximum acceptor incorporation, a donor blocked at the 5'-hydroxyl has been used to drive the reaction. It has also been shown that acceptor incorporation decreases as the chain-length increases up to the hexanucleotide. Synthesis of a 3'-blocked substrate for polynucleotide phosphorylase for use in chain termination studies was attempted using adenylate kinase. In this …

Synthesis And Metal Coordination Properties Of Some Cyclic Polyethers And Polythioethers, Barry Lant Haymore

Theses and Dissertations

Thermodynamic values for cation-cyclic polyether interactions for several systems are reported and discussed in light of ligand geometry, cation selectivity, solvent effects, structure, stoichiometry and the nature of the coordinate bond. Cyclic polyethers are shown to be remarkably good ligands even in water and to have marked selectivity for d0, d10 and d10 s2 cations. Syntheses of one new cyclic polyether and several new mixed cyclic polyether-thioethers are reported. Cyclic chelates with smaller rings are shown to prefer smaller cations, but this selectivity is masked by the hydration energy of the cation. Except for thiophillic cations such as Hg2+ and …

Acid-Base Titrations In Sulfolane, Francine O. Lawrence

Chemistry and Chemical Biology ETDs

A study of the solvent effects of sulfolane (tetrahydrothiophene 1, 1-dioxide, tetramethylene sulfone) upon a series of acids and several bases has been conducted. A procedure is presented for purifying sulfolane which results in a solvent of very low water content (5 x 10^-4 M) and very few impurities, as shown by its cooling curve. Potentiometric and conductometric measurements of additions of acids and bases to purified sulfolane and of various acid-base titrations were made. The series of carboxylic acids included benzoic, orthomethoxybenzoic, and three different monochlorobenzoic acids. The bases investigated included sodium methoxide, pyridine, and diphenylguanidine. Interpretation of …

Continuous Wave Co2 Laser Degradation And Mass Spectrometric Analysis Of Organic Polymers, Stanley G. Coloff

Chemistry and Chemical Biology ETDs

Previous laser-mass spectrometer and laser-gas-chromatographic instruments for study of organic polymer degradation have used high power pulsed lasers (ruby, Nd-glass) which limit sample characterization due to extensive fragmentation. A new experimental technique has been developed for high temperature pyrolysis and rapid analysis of organic polymers. The experimental technique combines a low power continuous wave CO₂ laser for sample pyrolysis and a time-of-flight mass spectrometer to analyze degradation products. The laser built for use in this study was a continuous flow CO₂ gas-molecular laser excited by high voltage direct current with maximum power of 10 watts at a wavelength …

On The Mechanism Of Low-Temperature Termolecular Atomic Recombination, Russell T. Pack, Richard L. Snow, Wesley D. Smith

Faculty Publications

A simple theory of gas-phase termolecular atomic recombination kinetics is presented which treats the bound-complex (BC) mechanism in a fashion which parallels the Roberts, Bernstein, and Curtiss resonance theory of the energy-transfer (ET) mechanism. The ET rate constant has a low temperature maximum, but the BC rate constant does not. The contributions of the two mechanisms to the low-temperature recombination of hydrogen atoms, with He, Ar, and H2 as third bodies, are estimated and compared. The presence (absence) of a low-temperature maximum in the observable total rate constant is determined by absence (presence) of bound vibrational levels of the hydrogen-third …

Studies On The Mechanism Of Action Of Nerve Growth Factor., D. (Daniel) B. Hier, B. G. Arnason, M. Young

Chemistry Faculty Research & Creative Works

No abstract provided.

Purification And Inhibition Study Of Xanthine Oxidase, Porn Paul Israngkun Na Ayudthaya

All Master's Theses

The paper describes a new and improved method of isolating and purifying xanthine oxidase and includes inhibition studies involving a number of reagents which are structurally related to the substrate or to other inhibitors of xanthine oxidase. The inhibitors studied are classified as to type, and inhibitor constants are reported.

The Hemoglobin Of Tubifex Tubifex, Lalitchandra Vrajlal Vora

Masters Theses

The hemoglobin of Tubifex tubifex is found in solution in the blood of the worm in cells as in vertebrates. The binding of oxygen to the hemoglobin can be followed spectrophotometrically. A peak of absorbtion at 540 nm is fairly constant under different condition, while a peak at 575 nm disappears from the spectrum when oxygen is removed. The hemoglobin contains heme, non-heme iron and protein.

Binding of oxygen to the hemoglobin was found to be dependent on the concentration of the hemoglobin, at low concentrations (4.16 x 10^-6 M), oxygen could not be removed from the hemoglobin. Only …

The Conductance Of Some Non-Rigid Bolaform Electrolytes, William Terry Schmidt

Masters Theses

Bolaform electrolytes are those which contain two or more charges separated by an inert framework. These are intermediate in structure between simple electrolytes and polyelectrolytes. Since these are similar to both synthetic and biological electrolytes, this investigation has been undertaken to determine some of their properties in solution.

The sodium and magnesium salts of ethane, propane, butane, pentane, hexane and decane α,ω-disulfonates were prepared. Each acid was prepared by refluxing the α,ω-dibromide of the desired salt with sodium sulfite. The sodium and magnesium salts were prepared by ion-exchange techniques.

The conductance of each salt was determined in methanol utilizing the …

Spectrophotometric And Microbiological Determination Of The Stability Of Sodium Ampicillin As Affected By Ph, Enrico Frank Raffanti

University of the Pacific Theses and Dissertations

The specific purpose of this investigation was to determine the effect of pH on the stability of ampicillin sodium for injection. Then, to correlate this information, a pH profile may be established to predict chemical stability with ampicillin sodium alone or when other drugs may be included in the admixture. With a pH profile the pharmacist could predict the length of time after which more than a 10 percent loss of activity would occur. Stability measurements were to be accomplished through a microbiological assay after adding ampicillin sodium to different buffer solutions. At the end of four hours, eight hours, …

The Utilization Of High Sensitivity Noble Gas Mass Spectrometry In The Detection Of Iodine-129 And Plutonium-244, Mervet S. Boulos

Doctoral Dissertations

"Mass spectrometric analysis of the xenon isotopes in natural gas wells demonstrates that radioactive isotopes of element number 53 (I) and element number 94 (Pu) were present at the time of the Earth's formation. Evidence for the now extinct nuclide¹²⁹I (t_1/2 = 17 x 10⁶ years) was found in CO₂ -rich gas while evidence for the now extinct ²⁴⁴Pu (t_1/2 = 82 x 10⁶ years) was discovered in other natural gas wells. The amounts of ¹²⁹I in thyroids was measured by combining neutron activation analysis with noble gas mass spectrometry. The current …

13th Annual Rocky Mountain Spectroscopy Conference

Rocky Mountain Conference on Magnetic Resonance

Program and abstracts from the 13th annual meeting of the Rocky Mountain Spectroscopy Conference, hosted by the Rocky Mountain Section of the Society for Applied Spectroscopy. Held in Denver, Colorado, August 9-10, 1971.

A Study Of A Divalent Cation-Selective Electrode, William Stanhope Ryan Jr.

Master's Theses

The ion-selective membrane electrode, recently introduced commercially, has shown excellent promise as a tool for determining ionic activities and concentrations in solution. It was the intent of the author to study the Orion divalent cation-selective electrode with two objectives.

The first objective of this research was to characterize the response of the divalent cation-selective electrode to calcium and magnesium ions in aqueous solutions. The effect of anions, monovalent cations; and pH upon this response was investigated. An examination was made of the response of this electrode to other divalent cations and of the effect of organic solvents on the membrane …

Analytical Aspects Of Laser-Induced Degradation Of Organic And Inorganic Compounds, William T. Ristau

Chemistry and Chemical Biology ETDs

The rapid deposition of energy by means of a pulsed ruby laser to degrade organic and inorganic materials is investigated as an analytical tool. Advantages and disadvantages of using a laser are discussed and compared to current conventional pyrolysis methods. The interaction of laser radiation with matter is briefly discussed. A number of different organic compounds including saturated and aromatic hydrocarbons, heterocyclic compounds, and polymers have been studied. The degradation products were separated and identified by use of a gas chromatograph. Degradation studies have also been done on a series of inorganic salts including nitrates, sulfates, thiosulfates, and oxalates. The …

The Synthesis Of Some New Condensed Pyridazines, Pyridazine N-Oxides And Pyrimidines As Potential Chemotherapeutic Agents, Donald E. Pichler

Chemistry and Chemical Biology ETDs

The need for new antimicrobial and antifungal agents as well as medicinals possessing antiprotozoal activity against special strains of malaria resistant to most synthetic antimalarials prompted the synthesis of new compounds that may have the desired antimetabolite properties. The structural similarities of the 1,2,5-thiadiazolo[3,4-d] pyridazines and the v-triazolo[4,5-d]pyridazines with the biologically important purines prompted the synthesis of members of these ring systems. The 1,2,5-thiadiazoles synthesized as intermediates ware also screened as potential chemotherapeutic agents. Similarly, the pyridazine N-oxides and the 5-nitro substituted pyrimidines are structurally similar to the pyrazine N-oxides and the pyridine N-oxides, which are known to possess a …

A Study Of The Acetic Anhydride Method For The Determination Of Citric Acid, Russell Kent Odland

Master's Theses

Citric acid is an important compound of many systems. Numerous methods, both qualitative and quantitative, have been used for the determination of the compound. Some of the biological systems investigated include fermentation studies, action on yeasts, antioxidant in fruits, additive in bakery goods and salad dressing preparations, amounts in vegetables, and others.The above examples are not complete; they only show the varied applications of citric acid. The purpose of this project was to investigate a quantitative spectrophotometric method using anhydrous conditions based on the citric acid-acetic anhydride-pyridine reaction. Conditions affecting the reaction were investigated and optimized for maximum sensitivity and …