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A Novel Class Of Metal-Directed Supramolecular Dna-Delivery Systems, Juan Noveron

Departmental Papers (Chemistry)

No abstract provided.

Chemical Analyses In Support Of Yucca Mountain Studies, Harry Reid Center For Environmental Studies, University Of Nevada, Las Vegas

Publications (YM)

Research Objectives: Provide preparation of injectates, sorption studies and analyses for tracer tests Provide REE, trace metals, majors and field measurement data from wells of the Nye County Early Warning Drilling Program (NCEWDP)and Inyo County Other laboratory needs as they arise

Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker

Electronic Theses and Dissertations

To date, the use of mitochondrial DNA in forensic analysis has relied on the presence of variations in the control region to differentiate between samples. One problem that this analysis has shown is the occurrence of common Haplogroup H haplotypes or identical sequences. Thus, there is a need to enhance the distinguishing power of this type of analysis. One option has been to investigate the mitochondrial coding region for polymorphisms that could differentiate between samples with identical control region haplotypes. The goal of this study has been to identify polymorphic coding region sites for development in a Pyrosequencing assay that …

Design And Performance Of A Curved-Crystal X-Ray Emission Spectrometer, A. C. Hudson, Wayne C. Stolte, Dennis W. Lindle, Renaud Guillemin

Chemistry and Biochemistry Faculty Research

A curved-crystal x-ray emission spectrometer has been designed and built to measure 2–5 keV x-ray fluorescence resulting from a core-level excitation of gas phase species. The spectrometer can rotate 180°, allowing detection of emitted x rays with variable polarization angles, and is capable of collecting spectra over a wide energy range (20 eV wide with 0.5 eV resolution at the Cl K edge) simultaneously. In addition, the entire experimental chamber can be rotated about the incident-radiation axis by nearly 360° while maintaining vacuum, permitting measurements of angular distributions of emitted x rays.

The Effects Of 17Β-Estradiol And Tamoxifen On Cell Cycle Distribution And F-Actin Expression In Mcf-7 Cells, Kirk Kangaloo, Latoya Jenkins, Nicole Clarke, Samir S. Raychoudhury

Journal of the South Carolina Academy of Science

No abstract provided.

Using Microenvironmental Effects: Loading, Activity, And Stereoselectivity Relative To Polymer Bound Nabh4 Reagents, James R. Blanton

Journal of the South Carolina Academy of Science

No abstract provided.

Schweinitz’S Sunflower Helianthus Schweinitzii Torrey And Gray, (Asterales: Asteraceae) In Upper Piedmont South Carolina, Steven E. Fields

Journal of the South Carolina Academy of Science

No abstract provided.

The Biophysical Analysis Of The Human Telomeric Repeat (Ttaggg)4 And Characterization Of Porphyrin Binding In Potassium Solutions, Cosimo Antonacci

Seton Hall University Dissertations and Theses (ETDs)

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Tumour Suppressor Function Of Rnase L In A Mouse Model, Wendy Liu, Shu Ling Liang, Hongli Liu, Robert Silverman, Aimin Zhou

Chemistry Faculty Publications

RNase L is one of the key enzymes involved in the molecular mechanisms of interferon (IFN) actions. Upon binding with its activator, 5′-phosphorylated, 2′-5′ oligoadenylates (2-5A), RNase L plays an important role in the antiviral and anti-proliferative functions of IFN, and exerts proapoptotic activity independent of IFN. In this study, we have found that RNase L retards proliferation in an IFN-dependent and independent fashion. To directly measure the effect of RNase L on tumour growth in the absence of other IFN-induced proteins, human RNase L cDNA was stably expressed in P-57 cells, an aggressive mouse fibrosarcoma cell line. Three clonal …

Exploring The Mechanism Of Flexible Biomolecular Recognition With Single Molecule Dynamics, H. Peter Lu, Qiang Lu, Jin Wang

Chemistry Faculty Publications

Combining a single-molecule study of protein binding with a coarse grained molecular dynamics model including solvent (water molecules) effects, we find that biomolecular recognition is determined by flexibilities in addition to structures. Our single-molecule study shows that binding of CBD (a fragment of Wiskott-Aldrich syndrome protein) to Cdc42 involves bound and loosely bound states, which can be quantitatively explained in our model as a result of binding with large conformational changes. Our model identified certain key residues for binding consistent with mutational experiments. Our study reveals the role of flexibility and a new scenario of dimeric binding between the monomers: …

Interaction Energy Decomposition In Protein–Protein Association: A Quantum Mechanical Study Of Barnase–Barstar Complex, Abdessamad Ababou, Arjan Van Der Vaart, Valentin Gogonea, Kenneth M. Merz Jr.

Chemistry Faculty Publications

Protein–protein interactions are very important in the function of a cell. Computational studies of these interactions have been of interest, but often they have utilized classical modelling techniques. In recent years, quantum mechanical (QM) treatment of entire proteins has emerged as a powerful approach to study biomolecular systems. Herein, we apply a semi-empirical divide and conquer (DC) methodology coupled with a dielectric continuum model for the solvent, to explore the contribution of electrostatics, polarization and charge transfer to the interaction energy between barnase and barstar in their complex form. Molecular dynamic (MD) simulation was performed to account for the dynamic …

Quantification Of Steroid Sex Hormones Using Solid-Phase Extraction Followed By Liquid Chromatography-Mass Spectrometry, Youssef Filali-Meknassi, Muriel Auriol, Craig D. Adams, Rao Y. Surampalli

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

In this study, the occurrence of trace amounts of natural and synthetic steroid estrogens in the aquatic environment was studied using liquid chromatography coupled with electrospray mass spectrometry, following solid-phase extraction (SPE). the SPE was performed with C18 and NH2 cartridges. the first objective was to develop a reliable method for analyzing steroid estrogens (resulting from human and animal excretions) in different matrices. the method developed was then applied to quantify the occurrence of natural and synthetic hormones (estrone [E1], 17β-estradiol [βE2], 17α-estradiol [αE2], estriol [E3], and 17α-ethinylestradiol [EE2]) in environmental samples in surface water and wastewater treatment plant (WWTP) …

Development Of Fluorescent Chemosensors Based On Energy Transfer In End-Capped Pi-Conjugated Systems, Huating Zhang

LSU Master's Theses

A novel sensory platform based on conjugated polymer was developed, that consists of a monodispersed oligo(phenylene ethynylene) backbone, end-capped with a terminal alkene for further attachment to a solid surface at one terminus, and an anthracene based receptor group at the other terminus. Energy transfer from backbone to end-cap was observed by absorption spectroscopy and fluorescence spectroscopy. Other ongoing projects described herein include the synthesis of longer-chain analogues of the above platform, the synthesis of silica microspheres based conjugated polymer sensory platform and the synthesis of long wavelength (NIR) dye for in vivo amyloid screening studies.

The Use Of A Chiral Fluorescent Resorcinarene For The Enantiomeric Recognition Of Amino Acids, Gerald I. Richard

LSU Master's Theses

The spectroscopic properties of a chiral boronic acid based resorcinarene macrocycle employed for chiral analysis were investigated. Specifically, the emission and excitation characteristics of tetraarylboronate resorcinarene macrocycle (TBRM) and its quantum yield were evaluated. The chiral selector TBRM was investigated as a chiral reagent for the enantiomeric discrimination of amino acids using steady-state fluorescence spectroscopy. Chiral recognition of amino acids in the presence of the macrocycle was based on diastereomeric complexes. Results demonstrated that TBRM had better chiral discrimination ability for lysine as compared to the other amino acids. Partial-least-squares regression modeling (PLS-1) of spectral data for macrocycle-lysine host-guest complexes …

Multifunctional Ligands For Supramolecular Chemistry, James Kakoullis

LSU Master's Theses

Metal-organic frameworks (MOFs) are the primary focus of the Maverick research group, almost exclusively with the acac moeity. Before such MOFs can be attempted, multifunctional ligands of the appropriate geometry for the desired MOFs first must be synthesized. In this thesis, the synthesis and characterization of two new β-diketone ligands, specifically, Re(CO)₃Cl(PyacH)₂, 2.2, Pphac₃H₃, 1.23, and its oxide 3.6, are described. Also in this thesis, attempts to synthesize other new MOFs will be discussed. The MOF for which Re(CO)₃Cl(PyacH)₂, 2.2 was designed was Re(CO)₃ClCu(pyac)₂] …

Landholder Information For The Safe Use And Management Of 1080, Renee Donovan, David Goodall

Biosecurity published reports

This manual outlines your roles and responsibilities for using and managing 1080. In particular this manual outlines the essential information you need to know in regard to:

• The legislative requirements for 1080 control in Western Australia
• The properties and mode of action of 1080
• How to lodge an application to lay 1080 baits How to safely store and transport 1080
• The procedures and notification required to carry out a baiting proposal
• Personal safety, identifying signs of poisoning & effecting appropriate first aid treatment
• Your responsibilities for notifying DAFWA and the Police of any accidents or incidents
• Methods for evaluating the …

Electron Affinities, Solvation Energies And Redox Potentials Of Some Chalcones: Substituents` Effect And Correlation With Semi-Empirical Mo Energies, Abdur Rahman, Rumana Qureshi, Mehvish Kiran, Farzana Latif Ansari

Turkish Journal of Chemistry

Experimental and theoretical investigations were carried out on 2 sets of chalcones. Set 1 has an OH group on ring A and Set 2 does not, and both have different substituents on ring B at different positions. The experimental investigations comprised cyclic voltammetric (CV) studies to determine the reactivity in terms of redox potentials in DMF at room temperature (23 \pm 1 °C). Electrochemical parameters from cyclic voltammograms were used to evaluate the reversibility of electron transfer, standard reduction potentials, solvation energies, electron affinities, diffusion coefficients, critical scan rates and heterogeneous electron transfer rate constants. Heterogeneous electron transfer rate constants …

Iridoid Glucosides From Lamium Garganicum Subsp. Laevigatum, Tayfun Ersöz, Duygu Kaya, Funda Nuray Yalçin, Cavi̇t Kazaz, Erhan Palaska, Charlott H. Gotfredsen, Soren R. Jensen, İhsan Çaliş

Turkish Journal of Chemistry

Phytochemical investigations on the above-ground parts of Lamium garganicum subsp. laevigatum resulted in the isolation of 7 iridoid glucosides: shanzhiside methyl ester (1), barlerin (8-O-acetylshanzhiside methyl ester) (2), 6-O-syringyl-8-O-acetylshanzhiside methyl ester (3), 6\beta-hydroxyipolamiide (4), lamalbide (5), dehydropenstemoside (6), and sesamoside (7). The structure of the iridoids was elucidated by spectroscopic (UV, IR, 1D- and 2D-NMR, and ESI-MS) evidence.

An Electrochemical Dna Biosensor For The Detection Of Dna Damage Caused By Radioactive Iodine And Technetium, Pinar Kara, Kağan Dağdevi̇ren, Mehmet Özsöz

Turkish Journal of Chemistry

A DNA damage process by radioactive iodine (I-131) and technetium (Tc-99m) is described. The procedure relies on a label-free electrochemical DNA biosensor. Fish sperm double stranded DNA was immobilized onto a carbon paste electrode (CPE) by electro deposition and the changes to the guanine oxidation signal were observed upon exposure to radioactive I-131 and Tc-99m by differential pulse voltammetry (DPV). The changes to the signal of guanine after exposure to therapeutic doses of Tc-99m and I-131 showed conformational changes in DNA molecules.

Synthesis, Characterization, And Biocidal Studies Of The Newly Synthesized Di- And Triorganotin(Iv) Complexes With N-Butylhydrogen Phthalate, Sadiq-Ur Rehman, Saqib Ali, Saira Shahzadi, Abdul Malik, Ejaz Ahmed

Turkish Journal of Chemistry

Di-(1, 3, 8) and triorganotin(IV) complexes (2, 5, 7) with the general formula R_nSnL_{4-n}, where R = Me, Et, n-Bu, n-Oct, Ph, and Bz, and L = the n-C_4H_9OCOC_6H_4COO^- monoanionic ligand have been synthesized by the reaction of the silver salt of the n-butylhydrogen phthalate with di- and triorganotin chloride in dry chloroform. However, di-n-butyl- and di-n-octyltin(IV) derivatives (4 and 6, respectively) were synthesized by the reaction of the corresponding organotin(IV) oxide with n-butylhydrogen phthalate in dry toluene. All the synthesized complexes were characterized by elemental analysis, FT-IR, multinuclear (^1H, ^{13}C, ^{119}Sn) NMR, and mass spectrometric techniques to assess the …

Selenium And Trace Element Distribution In Astragalus Plants: Developing A Differential Pulse Polarographic Method For Their Determination, Güler Somer, A. Cengi̇z Çalişkan

Turkish Journal of Chemistry

Astragalus plants have a wide range of applications in pharmaceuticals (gum tragacanth), as thickening agents in foods, and may have applications in controlling cancer cells. They are used as feed for animals and they are indicator plants for selenium. Because of their use in health-related areas it is very important to determine their selenium and trace element content with high accuracy. A new differential pulse polarographic method was established for trace element determination (10 elements) and their distribution in these plants. The Astragalus plants investigated in this work are Astragalus microcephalus and Astragalus lusitanicus, which grow almost everywhere. Their roots, …

Preparation, Spectroscopy, Antimicrobial Assay, And X-Ray Structure Of Dimethyl Bis-(4-Methylpiperidine Dithiocarbamato-S,S`)-Tin(Iv), Zia-Ur Rehman, Saira Shahzadi, Saqib Ali, Guo-Xin Jin

Turkish Journal of Chemistry

Dimethyl bis(4-methylpiperidine dithiocarbamato-S,S')-tin(IV) was synthesized and characterized by elemental analysis, IR and mass spectrometry, multinuclear NMR (^1H- and ^{13}C-NMR), and X-ray single crystal analysis. IR data showed that the ligand acts as a bidentate in the solid state. X-ray data showed the unsymmetrical nature of the ligand towards coordination to tin. It crystallized in the monoclinic P2_1/n space group. Its geometry is distorted octahedral. Antimicrobial activity data shows that the complex exhibits significantly more activity than the free ligand.

The Synthesis Of 2-(Chloromethyl)-6-Hydroxy-2h-Pyran-3 (6h)-One Via Achmatowicz Rearrangement, Zuhal Gerçek

Turkish Journal of Chemistry

Achmatowicz rearrangement was carried out with 2-chloro-1-(furan-2-yl) ethanol, which was synthesized starting from 1-(furan-2-yl) ethanone. It was shown that the oxidation of 2-chloro-1-(furan-2-yl) ethanol with m-CPBA produced 2-(chloromethyl)-6-hydroxy-2H-pyran-3 (6H)-one in good yield (70%).

Drift Tube Ion Mobility Measurements For Thermochemistry, Kinetics And Polymerization Of Cluster Ions, Ridha Ben Mohsen Mabrouki

Theses and Dissertations

In this work, the Drift Tube Ion Mobility technique is used to study the hydrophobic hydration and solvation of organic ions and measure the thermochemistry and kinetics of ion-molecule reactions. Furthermore, an exploratory study of the intracluster polymerization of isoprene will be presented and discussed. The ion hydration study is focused on the C3H3+ cation1 and Pyridine▪+ radical cation.2 The chemistry of the cyclic C3H3+ cation1 has received considerable attention and continues to be an active area of research.3-7 The binding energies of the first 5 H2O molecules to c-C3H3+ were determined by equilibrium measurements. The measured binding energies of …

Studies On The Anion Recognition Properties Of Synthesized Receptors Iii: A Novel Thiourea-Based Receptor Constructed By Benzo-15-Crown-5 For Sensing Anions In A Strong Polar Solvent, You-Ming Zhang, Hai-Xian Ren, Yan-Qing Zhou, Rong Luo, Wei-Xia Xu, Tai-Bao Wei

Turkish Journal of Chemistry

A new series of receptors were designed and synthesized, and their interactions with anions, such as F^-, Cl^-, Br^-, I^-, CH_3COO^-, HSO_4^-, and NO_3^-, in DMSO solvent were investigated using UV-Vis absorption spectroscopy. The results showed that hydrogen-bonding complexes were formed between the receptors and the tested anions, such as CH_3COO^- and F^-. It was also found that the selectivity of the receptors for anions could be efficiently tuned by changing the place of the substituent group at the N-phenyl moiety. The recognition mechanism and binding mode are discussed. These findings were expected to be of significance for designing and …

New Diterpenoids From The Aerial Parts Of Salvia Pilifera, Ufuk Kolak

Turkish Journal of Chemistry

Three new diterpenoids, piliferol (2\alpha, 20-dihydroxy-12-oxo- abieta-9(11),13-dien-8,20-ether) (1), salvipiliferol (2\alpha, 10\beta -dihydroxy-12-oxo-norabieta-9(11),13-dien) (2), and piliferalactone (2\alpha ,8-dihydroxy-12-oxo-abieta-9(11),13-dien-20-oic acid-8,20-lactone) (3), together with 7 known compounds, ursolic acid, oleanolic acid, betulinic acid, \alpha -amyrin, lupeol, \beta -sitosterol, and pectolinarigenin, have been isolated from the aerial parts of an endemic Salvia species, S. pilifera. Their structures were elucidated by spectral (UV, IR, 1D-, and 2D-NMR, and HRMS) methods.

Hyphenated Hplc-Ms Technique For Analysis Of Compositional Monosaccharides Of Transgenic Corn Glycoprotein And Characterization Of Degradation Products Of Diazinon, Fonofos And Aldicarb In Various Oxidation Systems, Tongwen Wang

Doctoral Dissertations

"The studies of this dissertation are composed of two sections. The first one deals with the analysis of compositional monosaccharides of transgenic corn glycoproteins. The method used in this study involved derivatization of monosaccharides with two fluorophores followed by HPLC/fluorescence detection for quantitative studies, and by HPLC/SSI/MS for identification confirmation of individual monosaccharide...The second section investigates the degradation processes of several pesticides including diazinon, fonofos and aldicarb in various oxidation systems"--Abstract, page iv.

Role Of Tridentate Schiff Base And Methoxyethylindenyl Derivatives Of Lanthanocene Complexes For The Synthesis Of High Molecular Weight Polymethyl Methacrylate (Pmma), Muhammad Yousaf, Aftab Ahmad Chatha, Abdul Jabbar, H. Badaruddin Ahmad, Ameer Fawad Zahoor, Muhammad Kaleem Khosa, Syed Sarwar Alam

Turkish Journal of Chemistry

Different mixed ligand lanthanocene complexes along with Al(i-Bu)_3 are capable of synthesizing partially syndiotactic high molecular weight polymethylmethacrylate. The Schiff base derivative of mixed ligand lanthanocene complexes (1-4) showed better efficiency (Mv = 227 \times 10^3-354 \times 10^3) compared to the methoxyethylindenyl derivative of mixed ligand lanthanocene complexes (5-9) (Mv = 72 \times 10^3, 172 \times 10^3). The effects of the catalyst, temperature, catalyst/MMA molar ratio, and catalyst/Al(i-Bu)_3 molar ratio on the polymerization of MMA at 60 °C suggested that ligand size and radius of rare earth metals play an important role in determining the activity of the catalyst.

Voltammetric And Spectroscopic Studies Of Vanadium(V)-- Nicotinamide Interactions At Physiological Ph, Semi̇ha Çakir, Ender Bi̇çer

Turkish Journal of Chemistry

The interaction of nicotinamide (NA) with NH_4VO_3 at the physiological pH (pH 7.4) was studied by voltammetry and UV-Vis spectroscopy. The reduction of V(V) ions complexed with NA in phosphate buffer (pH 7.4) was observed as a well-defined reversible reduction peak at --0.35 V. The stoichiometric (metal:ligand) ratio of the complex of V(V) with NA was determined as 1:2 by the mole ratio method.

Polymorphism Of Four Enantiotropic Crystalline Systems Containing Ni(Ii), H2O, 15-Crown-5 And No3-, Maxime Andre Siegler

University of Kentucky Doctoral Dissertations

The series of compounds [M(H₂O)₂(15-crown-5)](NO₃)₂, M = Mg, Mn, Co, Cu and Zn, has been extended to include two new phases for M = Fe and two new phases for M = Ni. The system [M(H₂O)₂(15-crown-5)](NO₃)₂ is remarkable for having many high-Z’ phases (Z’ > 1) with similar packing and for having solid-solid phase transitions through which there is no significant loss of crystallinity. The synthesis of the analogous Ni complex was carried out. Single-crystal X-ray diffraction showed …