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The Impact Of Dance On Physical, Intellectual, And Social Aspects Of College Life, Jenna Jean Hensen

Undergraduate Honors Thesis Collection

This study aimed to examine the physical, intellectual, and social impacts of dance on collegiate life. Members of the Butler University Dance Team were surveyed, and 3 members were interviewed in order to gain insight into how they perceived dance has impacted various aspects of their college life. Results showed that increased physical activity levels, academic motivation from teammates and coaching staff, as well as a positive relationship with teammates were positive aspects that dance team participants added to their college experience. Negatively, results showed lack of time for proper warm up, intense time commitments, and pressure to maintain a …

Investigation Into The Responses Of Physcomitrella Patens To Herbivory And The Influence Of Light On The Production Of Defense Compounds, Matthew Morris

Undergraduate Honors Thesis Collection

Plant defense mechanisms are well studied in many agriculturally relevant crops such as corn and tomatoes. Though less studied, the more ancestral nonvascular plants may be able to provide insights into the origin and working of modern plant defense systems. In this study, the bryophyte Physcomitrella patens was researched and the role of the jasmonic acid pathway in response to herbivory was investigated. Additionally, the impact of light intensity on the efficacy of this pathway was to be determined. After exposing the moss to various chemical elicitors and mechanical wounding, the activity of proteins produced in the JA pathway was …

Analysis Of Indianapolis Waterways For Evidence Of Eutrophication And Contamination Induced By Urban Pollution, Kiley Grace Wardwell

Undergraduate Honors Thesis Collection

The focus of my research will be largely focused on the water quality of the White River throughout Indianapolis. I will be testing water samples from five different points along the White River to analyze three of its contents, assessing the differing levels of pollution along different points progressing down the river and through the city. The locations I have chosen to test coincide with water gauge sites along the White River and are as follows: Logan Street in Noblesville, 82nd Street, Broad Ripple Dam, Michigan Road and Raymond Street. The surface water at these five locations will be tested …

Agricultural Practices And Nitrogen Cycling Microbes: The Impact Of Sustainable Farming Practices On Nutrient Movement In Indianapolis Urban Farms, Margaret Davis

Undergraduate Honors Thesis Collection

As population movement to urban centers and decreased availability of fresh foods becomes more common, the prevalence of food deserts is becoming far greater. Urban farming can potentially help address these issues by bringing healthy and fresh food sources directly to these areas that lack access to quality food. Urban farming is reliant on the implementation of sustainable practices, like the use of cover crops, to increase the amount of nutrients that are accessible to plants from the soil. Nitrogen is a vital nutrient but cannot be readily produced by plants, so it must be obtained by either an external …

Size Regulation Within The Germline Of The Developing Egg Chamber, Zoe Gladd Herdman

Undergraduate Honors Thesis Collection

The relationship between the size of a cell and the size of its intracellular structures must be precisely regulated for proper development and functioning of the mature organism; however, the mechanisms that establish and maintain this relationship are not known. Much of the work on organelle size scaling has focused on individual cells or organelles, with some looking at how size scaling is maintained during development. However, it is not known whether the size of multiple organelles is coordinately regulated, and how cell size and organelle size are regulated within a syncytium or group of connected cells. Because many cell …

Using Community Science To Assess The Effect Of Wing Pattern And Weather On Butterfly Behavior, Abbigail Merrill

Animal Science Undergraduate Honors Theses

Signaling in insects is used as communication and for attraction of mates. The physical appearance of the insect as well as conditions such as weather can play a role in visual signaling, by influencing the wavelengths of light available, and subsequent signal detection. We do not know, however, whether signals butterflies present broadly correlate with how they behave. In this study, we looked at the wing patterns and behavior of butterflies in Northwest Arkansas over a 3.5-year period to assess the relationship between wing pattern, weather, and behavior. We used observational data collected by hundreds of University of Arkansas students …

Targeting Prosthetic Joint Infections Using Modified Egcg Derivatives With Antibiotics, Kavneet Chahil

Theses, Dissertations and Culminating Projects

Prosthetic joint implantations have revolutionized orthopedic medicine by allowing individuals to regain physical function with minimal side effects. However, they are susceptible to post- surgical complications due to bacterial infections. Three significant bacteria that are responsible for the development of joint infections include Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa, which act by adhering to the prosthesis and form biofilms thus becoming antibiotic-resistant. It has been reported that green tea polyphenols, which are extracted from the leaves of the Camellia sinensis plant, have antibacterial, antioxidant, and anti- inflammatory properties. The purified, and modified epigallocatechin-3-gallate-stearate (EGCGS) and palmitoyl-epigallocatechin-3-gallate (P-EGCG) are more …

Assessment Of The Asexual Reproductive Strategies Of The Invasive Clinging Jellyfish Gonionemus Vertens, Valin Jarred Booker

Theses, Dissertations and Culminating Projects

Anthropogenic activities are dispersing organisms at higher rates and this dispersal leads to organisms having a higher chance at becoming invasive. The Clinging Jellyfish, Gonionemus vertens, was originally from the north Pacific Ocean, but was identified in New Jersey’s coastal waters in 2016. G. vertens undergo a complex life history with an asexual polyp stage and the sexual medusa stage. Little is known about their polyp stage, which shows multiple asexual reproductive strategies that can generate high densities of medusa. Asexual reproduction of polyps was observed in 2020 to assess the rate of clonal production. Cultured polyps can have several …

Cas9 Grna And Homologous Recombinant Plasmid Integrate Inducible Ha-N-Icer In The Aavs1 Locus Of Sk-Mel-24 Cells, Douglas Job

Theses, Dissertations and Culminating Projects

Melanoma is one of the most aggressive forms of cancer and is prone to metastasis. Once melanoma metastasizes, it is difficult to treat as patients often become immune to MAPK inhibitors and other therapeutic agents. Small molecules, such as immune checkpoint inhibitors, have demonstrated success as an adjunct to melanoma treatment. As a small, 18kDa molecule with tumor suppressing properties, research using ICER continues to provide positive anti-tumor effects. ICER regulates cAMP-induced transcription as a powerful, dominant-negative transcriptional repressor. Recent research demonstrated that the fusion tag hemagglutinin (HA), when fused to ICER’s N-terminus, increased ICER’s half-life and apoptotic activity in …

Turning Tadpoles: Effects Of Salinity And Lateralization On Hyla Versicolor Escape Behaviors, Victor Lopiccolo

Theses, Dissertations and Culminating Projects

Road salt, which is used as a roadway deicer in the winter time, is a major contributor to the salinization of freshwater habitats. Amphibians are particularly vulnerable to the salinization of aquatic environments due to the reliance of many species on pond, lake, and stream habitat in their aquatic larval stage. This study examined how Northern Gray Treefrog (Hyla versicolor) tadpole behavior is affected by salinity in a multiple t-maze. Alternate turning is sequential turning in opposite directions. Alternating turns lead the animal further from the starting point, and are innate behaviors associated with foraging, exploration, and escape. A multiple …

Spatial Distribution And Recruitment Of The Botrylloides Violaceus And Botryllus Schlosseri Tunicates In Barnegat Bay, New Jersey, Kaitlyn Maguire

Theses, Dissertations and Culminating Projects

Botrylloides violaceus and Botryllus schlosseri, colonial ascidians, are known invasive species of the North American Atlantic coast. The tunicates take residence and grow on native eelgrass (Zostera marina) blades, potentially negatively affecting eelgrass growth rate by means of smothering which reduces the plants ability to photosynthesize. Zostera marina is an important habitat, food source, and an indicator of environmental health. Invasive tunicates can decrease the amount of light that eelgrass receives which in turn, decreases the rate of growth of eelgrass. In the summer months of 2021, the relationship and abundance between the invasive tunicates B. schlosseri and B. violaceus …

Effect Of Soil Conditions On Invasive Purple Loosestrife Compared To Native Species, Madeline Ruth Baum

Theses, Dissertations and Culminating Projects

Purple loosestrife (Lythrum salicaria) is an invasive plant that has degraded many wetland habitats since its initial introduction to the United States in the early 19th century. To aid in managing purple loosestrife, it is important to study how purple loosestrife grows under variable environmental conditions compared to common native species, which can help managers understand which habitats are most at-risk of invasion. High or low salinity and pH are often barriers to plant growth, and the stress caused by these environmental conditions can cause plants to deplete their starch reserves. If native species are more negatively affected by stressful …

Green Tea Polyphenols Egcg-S And P-Egcg Containing Formulations Effectively Inhibit Growth And Biofilm Formation Of Eskape Bacteria, Calvin William Taylor

Theses, Dissertations and Culminating Projects

The field of medical microbiology has prospered for decades, finding treatments and cures for a multitude of bacterial infections. While success has been found in certain areas, challenges have arisen along the way. One of those challenges being the group of bacteria known as ESKAPE. The ESKAPE bacteria (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) have developed their multidrug resistance through adaptation to excessive drug use or prescription, unnecessary antimicrobial utilization, and substandard pharmaceuticals. They are the major cause of nosocomial infections throughout the world. With the ever-growing concern of antibiotic resistance in treating …

Evaluation Of Ranavirus Major Capsid Protein Gene Sequence Mutations In United States East Coast Frog And Tadpole Populations, Erin Marie Tyll

Theses, Dissertations and Culminating Projects

Infectious disease is a leading cause of death in ectothermic taxa including amphibians, reptiles, and bony fish. Frog Virus 3 (FV3) Ranavirus is a prominent pathogen that infects frog and tadpole populations with lethal consequences, especially for pre-metamorphosed individuals. Wood frogs (Lithobates sylvaticus) are particularly susceptible to FV3 infection and mortality events. Conservation of this animal is a critical effort for the maintenance of these ecologically significant animals. Amphibians contribute to the regulation of insect pest levels, energy transfer between aquatic and terrestrial habitats, algae community productivity and function, and vertebrate biomass. Understanding genetic variations of FV3 is crucial to …

Isolation And Analysis Of Single-Stranded Dna Aptamers For The Detection Of Ranavirus, Stephanie Zapata

Theses, Dissertations and Culminating Projects

The current molecular diagnostic methods for detection of deadly pathogens within a population can be time consuming and expensive. When surveying a population for the presence of a disease, time is a valuable resource. Therefore, a rapid pathogen test to use in the field will allow for faster detection of disease that can lead to determining a course of action for conservation of a species. Ranavirus is a double-stranded DNA virus that can cause mass mortality events in amphibian, reptile and fish populations. The disease can have the potential to wipe out entire populations or species if not controlled, having …

Bioinformatics Analysis Of The Transcriptomic Profiles Of Duchenne Muscular Dystrophy Patients / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Bianca Gonzalez

Legacy Theses & Dissertations (2009 - 2024)

Duchenne Muscular Dystrophy (DMD) is a muscle-wasting disease that primarily affects boys characterized by loss of the dystrophin gene. Dystrophin is necessary for proper muscle growth, muscles that lack dystrophin are more prone to damage. In this thesis, a bioinformatics approach was used to evaluate the transcriptomic profiles of two datasets obtained from DMD patients. The two datasets were analyzed separately, as well as combined to identify differentially expressed genes. The first dataset, GSE6011, has 23 DMD samples and 14 controls. The second, GSE38417, consists of 16 DMD samples and six controls. Differential gene expression of both datasets combined found …

Translation Control Tunes Drosophila Oogenesis, Elliot T. Martin

Legacy Theses & Dissertations (2009 - 2024)

The decision of a stem cell to either self-renew or differentiate is controlled by specific cellularpathways that can act at the level of transcription, translation, or post-translation. To study the regulation of these pathways in-vivo, I have used the female Drosophila germline as a model system. Each of the steps from germline stem cell (GSC) to egg require changes in cellular pathways. These changes can occur at the level of transcription, post-transciption, translation, or post-translation . Decades of research has elucidated many of the changes to gene that occur during oogenesis, however, many players in this process still remain mysterious. …

Role Of H3k4 Methylation In Myogenesis, Regeneration, And Muscle Disease / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Hannah Emily Shippas

Legacy Theses & Dissertations (2009 - 2024)

Muscles are a crucial part of an organism’s wellbeing, as defects in distinct types of muscles can be lethal. Our voluntary muscles, skeletal muscles, go through distinct regeneration phases before reaching maturity. With its own pool of stem cells, muscles have a large potential for regeneration. This potential has led scientists into looking closely at the steps that lead a muscle stem cell into muscle fiber, also known as myogenesis. As a muscle cell moves through myogenesis, genes are led to be turned on/off with different epigenetic mechanisms, one being histone modifications. A methylation modification on the 4th Lysine on …

Exploring The Response To Arsenic Using Trna Modification Detection, Writer Mediated Protection And Codon Usage Analytics, Anwesha Sarkar

Legacy Theses & Dissertations (2009 - 2024)

Transfer ribonucleic acid (tRNA) is a non-coding RNA which interacts with the messenger RNA (mRNA) to facilitate protein synthesis. tRNA is also one of the most heavily modified RNAs with the collection of modifications, termed as the tRNA epitranscriptome, ranging from simple methylation to complex hypermodification catalyzed by RNA epitranscriptomic writers. Detecting tRNA modifications is an evolving field and the state-of-the-art technologies that include next generation sequencing and mass spectrometry of intact and derivatized RNAs is described in the chapter one. Liquid chromatography coupled with mass spectrometry has previously been used to show that arsenic promotes increases in the levels …

Temporal And Spatial Comparison Of Pseudo-Nitzschia Species Composition And Domoic Acid In Narragansett Bay, Rhode Island And The Northeast U.S. Shelf, Isabella Church, Bethany D. Jenkins

Senior Honors Projects

Diatoms are unicellular algae that make up a significant portion of phytoplankton biomass at the base of marine food webs. Narragansett Bay (NB), RI is home to several species of the diatom genus Pseudo-nitzschia (P-n), some of which are known to cause harmful algal blooms through the production of the potent neurotoxin domoic acid (DA). This toxin can lead to Amnesic Shellfish Poisoning in humans through the consumption of affected shellfish. Although the presence of P-n has been well established in NB for over 50 years, DA levels have only recently become a problem, with shellfish harvest closures …

Wildlife Associates Of Nine-Banded Armadillo (Dasypus Novemcinctus) Burrows In Arkansas, Brett A. Degregorio, John T. Veon, Andrhea Darleen Massey

Biological Sciences Faculty Publications and Presentations

The Nine-banded Armadillo (Dasypus novemcinctus) is a widespread burrowing species with an expanding geographic range across the southeastern and midwestern United States. Armadillos dig numerous, large burrows within their home ranges and these burrows are likely used by a diverse suite of wildlife species as has been reported for other burrowing ecosystem engineers such as Gopher Tortoises (Gopherus polyphemus), Desert Tortoises (Gopherus agassizi), and Black-tailed Prairie Dogs (Cynomys ludovicianus). We used motion-triggered game cameras at 35 armadillo burrows in 4 ecoregions of Arkansas and documented 19 species of mammals, 4 species of …

Vectors, Pathogens And Climate Change: How Will Human Health Be Affected?, Joseph T. Barry

Honors Projects

This paper details how ongoing climate change will continue to affect the future of humanity. More specifically, this paper seeks to begin a conversation about how climate change impacts vectors and vector-borne pathogens and how both of these ultimately impact the future of human health. Three different vectors and three different pathogens are compared to provide an overview of how climate change may benefit these vectors and their associated pathogens. The vectors species selected for this comparative study are: (i) mosquitoes (Diptera, family Culicine), (ii) ticks (superorder Parasitiformes, order Ixodida), and (iii) kissing bugs (Hemiptera, subfamily Triatominae). Yellow fever virus …

An Overview Of The Current Research On Epiphyte Ecology, Kelsey Inman-Carter

Honors Projects

Epiphytes are non-parasitic, photosynthetic organisms that depend on other photosynthetic host organisms for support. Epiphytes can be found both in aquatic and terrestrial habitats. The interactions between epiphytes and their hosts can vary based on both their habitat and the features of individual hosts. This review explores the many facets of epiphyte research, including: the diversity of epiphytes in aquatic and terrestrial habitats; the effects of host-epiphyte interactions on the hosts; and the abiotic and biotic interactions that together determine epiphyte community composition.

Tree Species Diversity Increases With Conspecific Negative Density Dependence Across An Elevation Gradient, Joseph A. Lamanna, F. Andrew Jones, Davod M. Bell, Robert J. Pabst, David C. Shaw

Biological Sciences Faculty Research and Publications

Elevational and latitudinal gradients in species diversity may be mediated by biotic interactions that cause density-dependent effects of conspecifics on survival or growth to differ from effects of heterospecifics (i.e. conspecific density dependence), but limited evidence exists to support this. We tested the hypothesis that conspecific density dependence varies with elevation using over 40 years of data on tree survival and growth from 23 old-growth temperate forest stands across a 1,000-m elevation gradient. We found that conspecific-density-dependent effects on survival of small-to-intermediate-sized focal trees were negative in lower elevation, higher diversity forest stands typically characterised by warmer temperatures and greater …

Evolved Resistance To A Novel Cationic Peptide Antibiotic Requires High Mutation Supply, Alfonso Santos-Lopez, Melissa J. Fritz, Jeffrey B. Lombardo, Ansen H.P. Burr, Victoria A. Heinrich, Christopher Marshall, Vaughn S. Cooper

Biological Sciences Faculty Research and Publications

Background and Objectives

A key strategy for resolving the antibiotic resistance crisis is the development of new drugs with antimicrobial properties. The engineered cationic antimicrobial peptide WLBU2 (also known as PLG0206) is a promising broad-spectrum antimicrobial compound that has completed Phase I clinical studies. It has activity against Gram-negative and Gram-positive bacteria including infections associated with biofilm. No definitive mechanisms of resistance to WLBU2 have been identified.

Methodology

Here, we used experimental evolution under different levels of mutation supply and whole genome sequencing (WGS) to detect the genetic pathways and probable mechanisms of resistance to this peptide. We propagated populations …

Food Quality, Security, And Thermal Refuge Influence The Use Of Microsites And Patches By Pygmy Rabbits (Brachylagus Idahoensis) Across Landscapes And Seasons, Peter J. Olsoy, Charlotte R. Milling, Jordan D. Nobler, Meghan J. Camp, Lisa A. Shipley, Jennifer S. Forbey, Janet L. Rachlow, Daniel H. Thornton

Biology Faculty Publications and Presentations

How intensely animals use habitat features depends on their functional properties (i.e., how the feature influences fitness) and the spatial and temporal scale considered. For herbivores, habitat use is expected to reflect the competing risks of starvation, predation, and thermal stress, but the relative influence of each functional property is expected to vary in space and time. We examined how a dietary and habitat specialist, the pygmy rabbit (Brachylagus idahoensis), used these functional properties of its sagebrush habitat—food quality, security, and thermal refuge—at two hierarchical spatial scales (microsite and patch) across two seasons (winter and summer). At the …

Seasonal Trends In Adult Apparent Survival And Reproductive Trade-Offs Reveal Potential Constraints To Earlier Nesting In A Migratory Bird, Kathleen R. Callery, John A. Smallwood, Anjolene R. Hunt, Emilie R. Snyder, Julie A. Heath

Biology Faculty Publications and Presentations

Birds aim to optimize resources for feeding young and self-maintenance by timing reproduction to coincide with peak food availability. When reproduction is mistimed, birds could incur costs that affect their survival. We studied whether nesting phenology correlated with the apparent survival of American kestrels (Falco sparverius) from two distinct populations and examined trends in clutch-initiation dates. We estimated apparent survival using multi-state mark-recapture models with nesting timing, nesting success, sex, age, and weather covariates. Nesting timing predicted the apparent survival of successful adults; however, the effect differed between populations. Early nesting kestrels had higher apparent survival than later …

Seasonal Trends In Adult Apparent Survival And Reproductive Trade-Offs Reveal Potential Constraints To Earlier Nesting In A Migratory Bird, Kathleen R. Callery, John A. Smallwood, Anjolene R. Hunt, Emilie R. Snyder, Julie A. Heath

Department of Biology Faculty Scholarship and Creative Works

Birds aim to optimize resources for feeding young and self-maintenance by timing reproduction to coincide with peak food availability. When reproduction is mistimed, birds could incur costs that affect their survival. We studied whether nesting phenology correlated with the apparent survival of American kestrels (Falco sparverius) from two distinct populations and examined trends in clutch-initiation dates. We estimated apparent survival using multi-state mark-recapture models with nesting timing, nesting success, sex, age, and weather covariates. Nesting timing predicted the apparent survival of successful adults; however, the effect differed between populations. Early nesting kestrels had higher apparent survival than later nesters in …

Evaluating Methods To Determine The Maximum Oxygen Consumption By The Gulf Killifish, Fundulus Grandis, Sylvia Mullen

Senior Honors Theses

Metabolic rate is an essential feature of animal physiology and ecology. The rate of aerobic metabolism, as determined by oxygen consumption rate (MO2), is influenced by a variety of factors, including body size, temperature, and activity levels. Maximum aerobic metabolic rate (MMR) reflects the physiological capacity of an animal for oxygen extraction and utilization. As such, MMR is argued to be an important feature of an animal’s life history. For fish, MMR is frequently estimated as the peak MO2 immediately following an exhaustive chase, although several studies indicate that this value may underestimate MMR. Rather, MMR may be attained during …

Functional Role Of Ppal And Potential For Moss In Industrial Applications., Susana Perez Martinez

Electronic Theses and Dissertations

This dissertation is an examination and characterization of the functional roles of PPAL. PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) is a recently discovered gene. PPAL homologs are present in all plants and many animals, where its function is largely unknown. It is possible that PPAL could participate in prenylation processes since it shares similarity to the α subunits of known prenylation enzymes. Prenylation is a post-translational modification of proteins that involves the addition of a lipid moiety to proteins to facilitate membrane targeting and association and promote protein-protein interactions. Prenylation has important roles in plant growth and development, including …