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Visualization Of Eye Development In Annual Killifish Using Whole-Mount Immunohistochemistry, Carmen Z. Rodriguez, Jason Podrabsky

Student Research Symposium

Vertebrate eye development is highly conserved. Current models have identified the gene networks involved in eye development, but lack the context of extreme environmental conditions that challenge these fundamental programs. Typical vertebrates live for many years while annual killifishes can complete their entire adult lifespan in a matter of weeks. Thus, annual killifishes are a strong model for age-related diseases such as age-related macular degeneration (AMD); an irreversible loss of central vision. Annual killifishes enter embryonic diapause; a period of developmental and metabolic dormancy. These embryos are extremely resistant to environmental stresses such as hypoxia/anoxia that would cause irreparable damage …

Survey Of The Bees And Floral Use At The Leach Botanical Garden, Joshua Page

Student Research Symposium

There have been few studies of the diversity of bee species found in Oregon’s urban areas, and fewer studies that have systematically examined how bee populations change over the seasons. A survey of native bee populations at the Leach Botanical Gardens was conducted to determine what bees were present, how they changed over the season, and what flowers they used. Biweekly over the 2020 and 2021 bee seasons, sections of the garden were sampled in 30 minute equal time intervals, divided among the patches of flowering plants on which bees were observed. Bee specimens were captured with collection vials on …

Exploring The Function Of Heart Regeneration In Survival Of Anoxia In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Connor Taylor Kime

Student Research Symposium

Cardiovascular disease is the leading cause of death in the world, half of which is caused by myocardial infarction (MI) or heart attack. Most of the damage resulting from MI is due to oxygen deprivation that leads to cell death. The resulting cardiac scarring due to MI is chronic in humans and is not yet treatable; the only method of restoration is heart transplantation. Other organisms, such as fishes, show great cardiac regenerative capacity. The annual killifish, Austrofundulus limnaeus, can survive for months without oxygen at certain developmental stages. Examining gene expression responses during anoxic exposure and recovery in …

Impacts Of Doliolids On The Marine Microbial Community Off The Oregon Coast, Melissa Steinman, Anne Thompson, Kelly Sutherland

Student Research Symposium

Marine microbes drive global biogeochemical cycling and are critical to sequestering carbon dioxide from the atmosphere to the ocean. Predation on marine microbes by viruses and single-celled microbes is well documented, yet models of microbial mortality with these sources do not account for observations of microbial death. We examined how an understudied group of animals, pelagic tunicates, could feed on marine microbes using an internal mucous mesh. One group of pelagic tunicates, the doliolids, have the potential to reshape marine microbial communities when they bloom. We sampled doliolids off the Oregon Coast and characterized the diversity, taxonomy, and relative abundance …

Discovery Of Truffles (Tuber Species) In North Mississippi Pecan Orchards, Scout Hodges

Honors Theses

This thesis describes a study conducted in the years 2019-2022, which aimed to discover a new species of truffle in the state of Mississippi. Tuber lyonii, more commonly referred to as the “pecan truffle,” is an ectomycorrhizal fungus found on the roots of pecan trees in much of the southeastern region of the United States. Though there are many truffles native to the United States, the pecan truffle is one of the few that has been found to have high culinary value. Given the plethora of pecan orchards in the state of Mississippi, I decided to search for evidence …

B-Vitamins Influence The Consumption Of Macronutrients In Honey Bees, Walaa Ahmed Elsayeh, Chelsea N. Cook, Geraldine A. Wright

Biological Sciences Faculty Research and Publications

Insects require dietary sources of B-vitamins, but relatively little is known about whether they regulate B-vitamin intake in the same way they regulate other nutrients. Honey bees meet their B-vitamin requirements mainly from the pollen they collect. Employing the geometric framework for nutrition, we found that honey bees actively regulate their vitamin intake following Bertrand’s rule. We fed bees with a diet of essential amino acids (EAAs) and carbohydrate (C) to identify how the addition of B-vitamins affected the regulation of these macronutrients. In our experiments, honey bees preferred vitamins in concentrations comparable to those found in honey bee food …

Effects Of Water Quality On Oyster Health On Georgia Coast, Laurel Henderson

Honors College Theses

ABSTRACT

Oysters are an economically and ecologically important shellfish species found along most coastlines. Along the Georgia coastline, oysters form extensive reefs, and are economically important as a food source. In addition, oysters perform a vital role in maintaining water quality due to their high filtration capacity in coastal waters. Oyster reefs form solid, 3-D substrate that prevent coastline erosion and provide structure for numerous other coastal species to grow, including many commercially and recreationally important species. Water quality factors, such as temperature and salinity, can influence oyster density, condition, and growth, and ultimately affect the services the reefs provide …

Development And Analysis Of Next-Generation Polymeric And Bio-Ceramic Based Orthopedic Scaffolds By Advanced Manufacturing Techniques, Sudeep K. Gummadi

ETD Archive

Gliomas express mutant isocitrate dehydrogenases producing excessive amounts of D 2-hydroxyglutarate (D2HG) and releasing some of it into the environment. The immune surveillance is reduced as a result, however, the mechanisms behind lymphocyte suppression by the D2HG stereoisomer remain unknown. I incubated Jurkat T cells with D2HG at concentrations present within and surrounding gliomas, or its obverse L2HG stereoisomer, and quantified 2HG isomers within washed cells by TSPC derivatization with stable isotope-labeled D2HG and L2HG internal standards, HPLC separation, and mass spectrometry. D2HG was found in quiescent cells in double the amount of L2HG. External D2HG or L2HG increased the …

The Potential Role Of Phenotypic Plasticity In The Ability Of Hydrocotyle Bonariensis To Occupy Two Different Habitats, Harold Ralph Parsons Iii

Honors College Theses

Phenotypic plasticity is the ability of an organism to change morphology and/or physiology in response to changes in the environment. Hydrocotyle bonariensis is a coastal perennial herb found in both coastal sand dunes and inland coastal plain habitats in Georgia. The purpose of this study was to determine if there were differences in leaf morphology for populations of H. bonariensis in coastal sand dune and inland coastal plain habitats. Leaf morphology and microenvironmental variables were compared between H. bonariensis populations at Tybee Island (TI), GA, representing the coastal sand dune habitat, and Georgia Southern University Armstrong Campus (GSU) in Savannah, …

Four In One: Cryptic Diversity In Geoffroy’S Side-Necked Turtle Phrynops Geoffroanus (Schweigger 1812) (Testudines: Pleurodira: Chelidae) In Brazil, V. T. De Carvalho, R. C. Vogt, R. R. Rojas, M. D. S. Nunes, R. De Fraga, R. W. Ávila, A. G J Rhodin, R. A. Mittermeier, Tomas Hrbek, I. P. Farias

Biology Faculty Research

Turtles are one of the most threatened groups of vertebrates, with about 60% of species classified at some level of extinction risk. Compounding this extinction crisis are cryptic species and species complexes that are evaluated under a single species epithet but harbor multiple species, each of which needs to be evaluated independently. The Phrynops geoffroanus species group is a classic example. Described first in 1812, it is currently thought to harbor multiple species. To test this hypothesis, we collected mitochondrial and nuclear genomic data, morphometric data, and distribution and associated biome information. We applied statistically rigorous species delimitation analyses, taxonomic …

Biol 352 Cell Biology: Journey From The Center Of The Cell, Meredith A. Rowe Ph.D.

Open Educational Resources

This project is intended to be a long-term, scaffolded project across an entire semester course. Due dates are spread throughout the semester, and points are distributed as the professor deems appropriate across the sections of the project.

Comparative Analysis Of Rna Tran-Scriptomes In Ptsd Patients, Tyler Forrest Chan

Honors Capstone Projects and Theses

No abstract provided.

Effects Of Glucocorticoids Upon Pro-Inflammatory Responses To Acute Sleep Fragmentation, Hunter Weaver

Masters Theses & Specialist Projects

Sleep loss is a common problem in humans who suffer from obstructive sleep apnea. Sleep aids in the regulation of immune responses, some of which induce inflammatory responses. Cytokines regulate the inflammatory process and are released in response to sleep fragmentation (SF) in mice. Glucocorticoids are hormones that are released from the adrenal cortices during a stress response and are considered to be anti-inflammatory and immunosuppressive at high doses but may stimulate immune function on an acute level. The first hypothesis tested was that normal physiological expression of glucocorticoids (Sham) will display increased IL-1β and TNFα expression levels, while high …

Factors Influencing Tree Growth In A Bottomland Hardwood Along The Green River In Kentucky, Caleb Miller

Masters Theses & Specialist Projects

In bottomland hardwood forests, large fluctuations in moisture availability can lead to stress from both flooded and drought conditions. These forests can have a complex species composition with different water use and drought strategies. The bottomlands in which these species co-occur can have a complex spatial distribution of alluvial sediments due to the meandering and migration of river or stream channels, which may impact the hydrology, nutrient dynamics, and productivity. At Western Kentucky University's Green River Preserve, approximately 9 ha of bottomland hardwood forest were planted in 2006. Although these hardwoods have received equal amounts of sunlight, rainfall, and wind, …

Simulating Polistes Dominulus Nest-Building Heuristics With Deterministic And Markovian Properties, Benjamin Pottinger

Undergraduate Honors Theses

European Paper Wasps (Polistes dominula) are social insects that build round, symmetrical nests. Current models indicate that these wasps develop colonies by following simple heuristics based on nest stimuli. Computer simulations can model wasp behavior to imitate natural nest building. This research investigated various building heuristics through a novel Markov-based simulation. The simulation used a hexagonal grid to build cells based on the building rule supplied to the agent. Nest data was compared with natural data and through visual inspection. Larger nests were found to be less compact for the rules simulated.

Mechanistic Basis For The Role Of Resistance And Tolerance Determinants In Antibiotic Treatment Failure, Misha Iqbal Kazi

Biology Dissertations - Archive

Failure of antibiotics in treatment of nosocomial Gram-negative bacterial infections has created a substantial burden for global public health. The β-lactam class of antibiotics includes last resort carbapenems, which are used to combat multidrug resistant Gram-negative bacterial infections. Production of β-lactamase enzymes that degrade these antibiotics is the primary mechanism β-lactam resistance in Gram-negative bacteria. While FDA-approved serine-β-lactamase inhibitors are co-formulated with β-lactam antibiotics to prevent their inactivation during treatment, no metallo-β-lactamase inhibitors have been approved for clinical use. The first study employs an innovative antimicrobial discovery platform to identify peptide inhibitors against the New Delhi metallo-β-lactamase-1 (NDM-1), which is …

Pif Transposable Elements Domestication And Genetic Novelty In Insects, Fatema Ruma

Biology Dissertations - Archive

Transposable elements or TEs, are selfish genetic elements that represent a major component of a eukaryotic genome. They are genetic units that can move and amplify within a host genome. Mobilization of these TEs can be harmful to the host genome as they can transpose to new locations and can be a source of disruptions to the genetic information, DNA breakage and ectopic recombination. It has been shown, however, that TE proteins can be domesticated/co-opted and supply functions for major adaptations. There are two broad classes of TEs: Class I or retro-transposable elements and Class II or DNA elements. It …

Evaluating Headstarting As A Conservation Strategy For Anegada Rock Iguanas (Cyclura Pinguis), Kelly Allison Bradley

Biology Theses - Archive

In an effort to combat the current extinction crisis, conservation biologists have developed a diverse set of tools, including conservation physiology. This scientific discipline applies physiological concepts and tools to generate conservation solutions for a broad range of species. The Anegada Rock Iguana (Cyclura pinguis) is a Critically Endangered species native to Anegada, British Virgin Islands. Feral cats depredate hatchlings within months of emergence, drastically reducing recruitment and population growth. By the 1990s, the population had declined by 80%. A headstart program was initiated in 1997 to combat this threat and increase the population. Wild collected hatchlings are reared in …

Global Mapping Of Protein-Nucleic Acid Interaction Of The Rle Line R2bm, Dhamala Santosh

Biology Theses - Archive

The R2 Long Interspersed Nucleotide Elements (LINEs) is a widely distributed site specific non-LTR retrotransposons that integrates exclusively into host genome’s 28s rRNA genes and replicate by a process called Target Primed Reverse Transcription (TPRT) which is a “copy-out, copy-in” mechanism whereby element protein binds to the mRNA from which they are translated, forming ribonucleic acid protein particles (RNPs). The investigation of nucleic acid-protein interactions using chemical modification of surface amino acid residues on a protein in the presence and absence of nucleic acids is a relatively new application/technique in the field of Mass Spectrometry and particularly significant in studying …

The Evolution Of Neurosensory Structures: Evaluating Ecological Drivers Of Brain And Eye Size Variation, Kaitlyn J. Howell

Biology Dissertations - Archive

Neurosensory structures (i.e., brain and eye size) vary extensively across taxa. The evolution of large neurosensory structures has long been an area of interest for scientists, but focus has largely been placed on macroevolutionary patterns at or above the species level. Few studies have investigated the role contrasting ecological factors play in the evolution of such structures within species. My dissertation examines 1) how divergent ecological pressures, particularly predation and competition, drive evolutionary shifts in brain and eye size within species and across sexes, and 2) why such variation evolves, particularly if larger brains drive shifts in behavior and fitness. …

Evaluation Of Diaphragmatic Movement In The Respiratory System Of An Emphysema Patient, Aubri Ford

Honor’s College Freeman Research Journal

Respiration is an essential part of life. The diaphragm is the main muscle involved in the process of respiration. However, respiratory diseases such as emphysema alter the function of the diaphragm which sometimes leads to respiratory failure and death. This review compiles studies concerning emphysema and how the morphological changes in the lungs (i.e. hyperinflation) caused by the disease affect the diaphragm. The first major point covers the risks associated with developing the disease as a component of Chronic Obstructive Pulmonary Disease (COPD). Next, the paper outlines the pathophysiology of emphysema within the lungs, the methods used to diagnose emphysema, …

Evidence That The Transcriptional Repressor Icer Is Regulated Via The N-End Rule For Ubiquitination, Angelo Cirinelli, Justin Wheelan, Christopher Grieg, Carlos A. Molina

Department of Biology Faculty Scholarship and Creative Works

ICER is a transcriptional repressor that is mono- or poly-ubiquitinated. This either causes ICER to be translocated from the nucleus, or degraded via the proteasome, respectively. In order to further studies the proteins involved in ICER regulation mass spectrometry analysis was performed to identify potential candidates. We identified twenty eight ICER-interacting proteins in human melanoma cells, Sk-Mel-24. In this study we focus on two proteins with potential roles in ICER proteasomal degradation in response to the N-end rule for ubiquitination: the N-alpha-acetyltransferase 15 (NAA15) and the E3 ubiquitin-protein ligase UBR4. Using an HA-tag on the N- or C-terminus of ICER …

The Effect Of A Health Educational Program On Sleep Related Health Outcomes, Swetha Sirigineedi

Biological Sciences Undergraduate Honors Theses

Sleep quality has effects on both physical and mental health. Because of this, sleep hygiene is an important health goal. Participation in health programs promoting healthy behaviors has helped people reach their health goals and elicited positive behavior change. Because of this, it was of interest to determine if participation in a virtual, multi-media health education program improved health and wellbeing outcomes of Arkansans. The main objective of this study was to determine the effect of a 20-week behavior and health educational program on the ability of participants to achieve their health goals and improve their health behaviors related to …

Life After Death – Does Carcass Biodiversity Scale With Carcass Body Size?, Troy Warfield

Biological Sciences Undergraduate Honors Theses

Mammals play a large role in the ecosystems where some, especially large-bodied mammals, act as ecosystem engineers. Mammal carcasses, particularly those of large body mass act as a temporary island of dense nutrients that support other organisms, including other mammal species, for an extended period. Research in this field currently focuses on the link between mammal carcass size and nutrient availably or on non-mammalian size and biodiversity, but little is available on the correlation between mammal carcass size and its influence on ecosystem biodiversity. Here we ask, does the available biomass (i.e., body size) of the carcass affect its role …

Seasonality Of Orthohantavirus Seroprevalence In Northwest Arkansas Rodents, Amy Schexnayder

Biological Sciences Undergraduate Honors Theses

Zoonotic viruses are viruses that can be transmitted from animals to humans. Rodent species are likely to be reservoirs for zoonotic viruses, and particular rodent-borne viruses, such as orthohantaviruses, may greatly threaten human health. Orthohantaviruses are a group of rodent-borne viruses that are at risk for spillover to human populations. Many aspects of orthohantaviruses have been well-researched, yet the seasonality of orthohantaviruses has not yet been thoroughly examined, especially in the southern United States. In this study, we captured 616 rodents trapped over 5953 trap nights across 13 grassland sites in Northwest Arkansas. Rodents were trapped for two consecutive nights …

Identifying The Distinct Adaptive And Plastic Gene Expression Patterns And Microbial Indicators That Mediate Coral Disease Resistance, Nicholas James Macknight

Biology Dissertations - Archive

Infectious diseases are an increasing threat to coral reefs, resulting in altered community structure and hindering the functional contributions of disease susceptible species. While forecasting disease outbreaks based on environmental factors has progressed, we still lack a comparative understanding of susceptibility among coral species that would help predict disease impacts on coral communities. This dissertation compared the phenotypic, microbial, and coral host gene expression responses of seven diverse Caribbean coral species after exposure to white plague disease. Disease incidence and lesion progression rates were evaluated over a seven-day exposure. Coral microbiomes and RNA were sampled after lesion appearance or at …

Evaluation Of Kappa Opioid Receptor Specific Compounds And Their Effect On Ptsd-Like Behavioral Activity, Daniel Murnock

Theses, Dissertations and Culminating Projects

Background: Post-traumatic stress disorder (PTSD) is a complex and multifaceted neurological disorder that is characterized by intrusive thoughts, avoidance behaviors, alterations in cognition and mood, and alterations in arousal and reactivity. Current studies utilize stress paradigms to mimic behavioral symptoms and neurobiological changes associated with PTSD. The project aims to instead utilize the kappa opioid system and three distinct Kappa Opioid Receptorspecific compounds (Salvinorin A, U50,488, and nor-BNI) to induce these changes. Pharmacokinetic analysis of Salvinorin A and U50,488 revealed a time of peak plasma concentration (Tmax) of 0.25 hours in both groups. 3-Chamber Social Novelty testing revealed a significant …

Keeping Your Cool: Thermoregulatory Performance And Plasticity In Desert Cricetid Rodents, Richard W. Ramirez, Eric A. Riddell, Steven R. Beissinger, Blair O. Wolf

Biology ETDs

Small mammals in hot deserts often avoid heat via nocturnality and fossoriality and are thought to have a limited capacity to dissipate heat using evaporative cooling. Research to date has focused on thermoregulatory responses to air temperatures (T_a) below body temperature (T_b). Consequently, the thermoregulatory performance of small mammals exposed to high air temperatures is poorly understood, particularly responses across geographic and seasonal scales. We quantified the seasonal thermoregulatory performance of four cricetid rodents (Neotoma albigula, N. lepida, Peromyscus eremicus, P. crinitus) exposed to high T_a, at four sites …

Feeding Preference Of The Sea Urchin Arbacia Punctulata For Algal Turf Over Kelp In A Degraded Kelp Forest Ecosystem, Kenneth J. Hamel

Theses, Dissertations and Culminating Projects

Increasingly severe ocean warming and marine heatwave events are driving declines in habitat-forming kelps worldwide. Direct mortality from extreme temperatures, coupled with destabilization of kelp forest communities, commonly trigger ecological community shifts from dominant kelp forests to an algal turf dominated state. Opportunistic algal turf species are more resilient to warming and lack the fundamental structure of kelp forests, and thus reduce ecosystem services and biodiversity. Once in place, algal turf communities contain ecological feedback mechanisms, diminishing kelp recruitment, and potential recovery. The various feedback mechanisms and ecological thresholds that maintain or drive community shifts between kelp forest and algal …

Fish Community Assembly At Multiple Spatial Scales In East Texas Streams, Jake R. Swanson

Electronic Theses and Dissertations

Stream communities are structured by environmental processes that vary in strength across different spatiotemporal scales. Therefore, revealing how stream fish communities are influenced by environmental gradients may clarify how communities respond to disturbance associated with anthropogenic change. I investigated the relative role environmental variables play in the processes that structure the taxonomic and functional diversity of stream fish communities, as well as historical trends in occurrence and local habitat associated with a species of greatest conservation need within east Texas. The results of this study indicate that stream sites with more habitat complexity and stability support a more taxonomically and …