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Secondary Analysis Of Chip-Seq Dataset And Genomic Interactions Of Icer, Karem Rivera Jan 2023

Secondary Analysis Of Chip-Seq Dataset And Genomic Interactions Of Icer, Karem Rivera

Theses, Dissertations and Culminating Projects

The Inducible cyclic AMP (cAMP) Early Repressor (ICER) is an endogenous transcriptional repressor of the cAMP-mediated signaling gene transcription pathway and belongs to the CRE-binding protein (CREB)/CRE modulator (CREM) gene family. ICER is associated to many functions and biological processes in the immune system, brain, regulation of spermatogenesis, circadian control of transcription, apoptosis and metabolic functions, and of particular interest, has been shown to act like a tumor suppressor. Recent findings from our laboratory suggest that ICER might have a role in DNA repair, but further experiments need to be performed. In this research, we aimed to elucidate the function …

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Assessing The Relationship Between Binding Kinetics And Subcellular Protection Of Ribosomes By Single-Domain Antibodies, Mara Bureau Jan 2023

Assessing The Relationship Between Binding Kinetics And Subcellular Protection Of Ribosomes By Single-Domain Antibodies, Mara Bureau

Legacy Theses & Dissertations (2009 - 2024)

Assessing the ability of VHHs to protect ribosomes from the depurination activity of Ricin toxin’s(RT) enzymatic subunit, RTA, can provide insights regarding binding placement, epitopes of interest and role of affinity in neutralization is critical for the development of anti-toxin countermeasures. Determining the level of correlation between binding strength, binding location and elicited protection can be useful for future and ongoing research. Ricin is easily manufactured and considered one of the most toxic biological agents known. Without an approved vaccine, continued research to design and optimize therapeutic countermeasures is essential. Over the past decade single-domain antibodies (VHHs) have been recognized …

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Olfaction And Puberty : The Study Of The Development And Migration Of The Gnrh-1ns And Their Migratory Partners The Olfactory Unsheathing Cells, Ed Zandro Moina Taroc Jan 2023

Olfaction And Puberty : The Study Of The Development And Migration Of The Gnrh-1ns And Their Migratory Partners The Olfactory Unsheathing Cells, Ed Zandro Moina Taroc

Legacy Theses & Dissertations (2009 - 2024)

Sexual development and puberty are two key developmental processes that are crucial for the propagation and evolution of species. The hormone Gonadotropin releasing hormone-1 (GnRH-1) is a key hormone in regulating both processes by controlling the hormonal Hypothalamic-Pituitary-Gonadal axis in the release of sex hormones (Testosterone, Estrogen, Progesterone) at the correct developmental time points initiate puberty and progress sexual development. A subset of hypothalamic neurons known as the GnRH-1 neurons (GnRH-1ns) release GnRH-1 in a pulsatile fashion during embryonic and post-natal development to specifically regulate these processes. The GnRH-1ns are an interesting group of neurons because they are formed in …

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Endothelial And Stromal Cell Contributions To Salivary Gland Injury And Repair, Amber Altrieth Jan 2023

Endothelial And Stromal Cell Contributions To Salivary Gland Injury And Repair, Amber Altrieth

Legacy Theses & Dissertations (2009 - 2024)

Injury and disease can contribute to excess accumulation of extracellular matrix, which if persistent, results in fibrosis, impeding proper organ function. The salivary gland can develop fibrosis following irradiation treatment for cancer therapy, Sjögren’s Disease, and other causes; however, it is unclear which stromal cells and signals participate in injury responses and disease progression and which cells may be important for restoring proper organ function. Two stromal populations that are implicated in injury and repair are the endothelial cells, lining the inner surface of blood vessels, and pericytes, that wrap around the vessels. One stromal pericyte population, expressing the transcription …

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Regulatory Mechanisms In The Drosophila Germline, Shane Breznak Jan 2023

Regulatory Mechanisms In The Drosophila Germline, Shane Breznak

Legacy Theses & Dissertations (2009 - 2024)

To launch the next generation, all sexually reproducing animals must undergo the process of producing mature eggs and sperm. This process is known as gametogenesis. During gametogenesis key developmental checkpoints are met to ensure proper egg and sperm production. Model organisms, such as Drosophila melanogaster, have been used to study gametogenesis to gain insight into the conserved mechanisms that can be attributed to human infertility and disease due to the availability of genetic tools, quick life cycle and the conservation of regulatory mechanisms. During Drosophila oogenesis there are many key developmental transitions that must be completed to produce a fully …

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Distribution Patterns And Factors Influencing Relative Abundance Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Mississippi, Luke Pearson, Lucas Haralson, Gabrielle Berry, Grover J. Brown, Carl Qualls Jan 2023

Distribution Patterns And Factors Influencing Relative Abundance Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Mississippi, Luke Pearson, Lucas Haralson, Gabrielle Berry, Grover J. Brown, Carl Qualls

Research, Publications & Creative Work

Macrochelys temminckii (Alligator Snapping Turtle) was recently proposed as a threatened species under the US Endangered Species Act due to current and projected future declining populations range wide. Despite a presumed statewide distribution in Mississippi, the Alligator Snapping Turtle had been documented in only 32 of 82 counties between 1857 and 2012, thus leaving a substantial gap in our knowledge of this species’ distribution. Therefore, we collected credible records from museums, literature, and the general public, as well as directly assessed the current distribution and relative abundance of the Alligator Snapping Turtle in all major river systems (i.e., Pascagoula, Pearl, …

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Gross Ecosystem Product Accounting Of A Globally Important Agricultural Heritage System: The Longxian Rice–Fish Symbiotic System, Jianfang Liu, Qian Zhang, Qianyi Wang, Yaoping Lv, Yingqi Tang Jan 2023

Gross Ecosystem Product Accounting Of A Globally Important Agricultural Heritage System: The Longxian Rice–Fish Symbiotic System, Jianfang Liu, Qian Zhang, Qianyi Wang, Yaoping Lv, Yingqi Tang

Research, Publications & Creative Work

The Rice–Fish Symbiotic System in the Longxian village is the first Globally Important Agricultural Heritage System (GIAHS) in Asia. This paper examined the value transformation mechanism of the Longxian Rice–Fish Symbiotic System from the micro perspective of material cycle and energy flow and quantified its economic value using the gross ecosystem product (GEP). Using the InVEST model and ArcGIS tools, the GEP was estimated to be approximately CNY 116.51 million (approx. CNY 252,624 per hectare per year). The ecosystem regulating service value occupied more than 80% of the GEP. Within the regulating ecosystem services, the climate regulation value was the …

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Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski Jan 2023

Winter Dynamics Of Storm Water Management Ponds And Winter Tolerance In Three Aquatic Plant Species, Patrick Strzalkowski

Theses and Dissertations (Comprehensive)

The vast majority of the research into the performance of stormwater management ponds (SWMPs) has been performed in warm regions or during the warmer seasons in temperate regions. It is presumed that SWMPs are inactive in the winter as any potential stormwater is trapped in snow and ice. The main goal of this thesis was to test this presumption and to study the dynamics and performance of three SWMPs during the winter. Remote water level loggers were installed into the three SWMPs and daily grab samples from the influents and effluents were taken and analyzed for total phosphorus (TP), chloride, …

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The Protective Impact Of Growth Hormone Against Rotenone-Induced Apoptotic Cell Death Via Acting On Endoplasmic Reticulum Stress And Autophagy Axis, Özge Rencüzoğullari, Selay Tornaci, Yağmur Çeli̇k, Nayat Narot Taş, Pinar Obakan Yerli̇kaya, Eli̇f Damla Arisan, Ajda Çoker Gürkan Jan 2023

The Protective Impact Of Growth Hormone Against Rotenone-Induced Apoptotic Cell Death Via Acting On Endoplasmic Reticulum Stress And Autophagy Axis, Özge Rencüzoğullari, Selay Tornaci, Yağmur Çeli̇k, Nayat Narot Taş, Pinar Obakan Yerli̇kaya, Eli̇f Damla Arisan, Ajda Çoker Gürkan

Turkish Journal of Biology

Human growth hormone (GH) is crucial modulator of cellular metabolisms, including cell proliferation and organ development, by stimulating insulin-like growth factor-1 (IGF-1), which has various functions such as cell proliferation, tissue growth, survival, or neuroprotection. Therefore, GH is implicated as a critical player in the cell and can enhance neurogenesis and provide neuroprotection during the treatment of neurological diseases such as Parkinson's disease (PD). In this study, the neuroprotective role of GH was investigated in rotenone-induced PD models for the first time. Both SH-SY5Y and SK-N-AS neuroblastoma cells were exposed to rotenone to mimic PD pathogenesis as stated in previous …

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Functional Enhancement Of Acute Infracted Heart By Coinjection Of Autologous Adipose-Derived Stem Cells With Matrigel, Baozhu Wang, Mengmeng Wang, Yan Li, Meihua Shao, Dan Zhan, Shiqi Yan, Xiang Ma, Yitong Ma Jan 2023

Functional Enhancement Of Acute Infracted Heart By Coinjection Of Autologous Adipose-Derived Stem Cells With Matrigel, Baozhu Wang, Mengmeng Wang, Yan Li, Meihua Shao, Dan Zhan, Shiqi Yan, Xiang Ma, Yitong Ma

Turkish Journal of Biology

Recent clinical developments in tissue bioengineering have applications in acute cardiac ischemia and infarction and include the use of stem cells that combine injectable scaffold material. This study aimed to evaluate the effects of adipose-derived stem cells (ADSCs) that combine the Matrigel scaffold on cardiac morphology/functions. The autologous ADSCs myocardial infarction (MI) model was induced by the permanent ligation method of the left anterior descending coronary artery (LAD). MI-operated rats were randomly divided into PBS group, Matrigel group, PBS plus ADSCs group (PBS+ADSCs), and Matrigel plus ADSCs group (Matrigel+ADSCs). Matrigel was used as an injectable scaffold. Rats with a 1-week-old …

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Tsrna-15797-Modified Bmsc-Derived Exosomes Mediate Lfng To Induce Angiogenesis In Osteonecrosis Of The Femoral Head, Shanhong Fang, Mengqiang You, Jie Wei, Peng Chen Jan 2023

Tsrna-15797-Modified Bmsc-Derived Exosomes Mediate Lfng To Induce Angiogenesis In Osteonecrosis Of The Femoral Head, Shanhong Fang, Mengqiang You, Jie Wei, Peng Chen

Turkish Journal of Biology

Background: Osteonecrosis of the femoral head (ONFH) is an ischemic disease characterized by the impairment of angiogenesis. We have previously elucidated the role of tsRNAs and BMSC exosomes in ONFH, but whether tsRNA-modified BMSC exosomes promote angiogenesis in ONFH remains unclear. Methods: The expression of angiogenesis-related tsRNA in plasma exosomes from ONFH patients was examined by q-PCR. The function of tsRNA in HUVECs was identified by CCK-8 and angiogenesis assay. Exosomes purified from tsRNA-15797 overexpressed BMSCs were cocultured with HUVECs to examine their role in angiogenesis. The molecule mechanism of tsRNA-15797-modified exosomes was explored by RNA sequencing, dual-luciferase assay, and …

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Mir-216b-5p Promotes Late Apoptosis/Necroptosis In Trastuzumab-Resistant Sk-Br-3 Cells, İştar Burcu Dolapçi, Senem Noyan, Ayşegül Yücel Polat, Hakan Gürdal, Bala Gür Dedeoğlu Jan 2023

Mir-216b-5p Promotes Late Apoptosis/Necroptosis In Trastuzumab-Resistant Sk-Br-3 Cells, İştar Burcu Dolapçi, Senem Noyan, Ayşegül Yücel Polat, Hakan Gürdal, Bala Gür Dedeoğlu

Turkish Journal of Biology

Breast cancer is the most common cancer in women. The human epidermal growth factor receptor 2 (HER2) overexpressing subtype is related to poor prognosis with an aggressive phenotype and is reported as one of the most commonly seen subtypes. Trastuzumab is prevalently used as a treatment method for HER2+ breast cancer however, resistance to the drug frequently occurs following the treatment. MicroRNAs (miRNAs) are 19-23 nucleotide long small RNAs, which regulate gene expression at posttranscriptional level and studies show that there are differentially expressed miRNAs between drug sensitive and resistant groups, indicating that they might have some key roles in …

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The Impact Of Nutrient Depletion On The Effect Of Hhq On Emiliania Huxleyi, Marley Gonsalves Jan 2023

The Impact Of Nutrient Depletion On The Effect Of Hhq On Emiliania Huxleyi, Marley Gonsalves

Honors Theses and Capstones

Emiliania huxleyi is a ecologically important, bloom-forming coccolithophore. HHQ is a bacteria compound that causes cell stasis in E. huxleyi that can lead to bloom termination. This study analyzed the role that nutrient depletion plays in this relationship. Results show that nitrogen depletion protects cells from the effects of HHQ, affecting bloom dynamics.

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Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher Jan 2023

Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher

Honors Theses and Capstones

24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These …

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Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery Jan 2023

Sustainability Practices In Aquaculture: Using Algae Turf Scrubber Biomass To Raise Black Soldier Flies As An Alternative Feed In Blue Tilapia, Oreochromis Aureus, Culture, Michelle C. Lowery

College of Graduate Studies: Theses & Dissertations

Since the 1980s, non-algal aquaculture has grown to encompass 49% of all seafood production in response to a growing human population and increased seafood demand (FAO, 2022). Hurdles exist to aquaculture sustainability, including dependence on wild sourced fishmeal (FM) and the impacts wastewater discharge. It takes 4-5 tons of wild forage fish to produce one ton of dry FM (Miles and Chapman, 2006) and as aquaculture is primarily conducted in earthen ponds and public open water bodies (FAO, 2022), finfish culture can have a high impact on the surrounding environment by discharging excess nutrients. This study used algae turf scrubber …

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Diversity Of Antimicrobial Peptides In Silkworm, Pooja Makwana, Kamidi Rahul, Katsuhiko Ito, Bindu Subhadra Jan 2023

Diversity Of Antimicrobial Peptides In Silkworm, Pooja Makwana, Kamidi Rahul, Katsuhiko Ito, Bindu Subhadra

Faculty Publications - Biological Sciences

Antimicrobial resistance is a phenomenon that the present-day world is witnessing that poses a serious threat to global health. The decline in the development of novel therapeutics over the last couple of decades has exacerbated the situation further. In this scenario, the pursuit of new alternative therapeutics to commonly used antibiotics has gained predominance amongst researchers across the world. Antimicrobial peptides (AMPs) from natural sources have drawn significant interest in the recent years as promising pharmacological substitutes over the conventional antibiotics. The most notable advantage of AMPs is that microorganisms cannot develop resistance to them. Insects represent one of the …

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Microdebris Abundance, Distribution, And Ingestion By Sargassum-Associated Juvenile Fishes In The Gulf Of Mexico, Olivia L. Lestrade, Frank Hernandez Jan 2023

Microdebris Abundance, Distribution, And Ingestion By Sargassum-Associated Juvenile Fishes In The Gulf Of Mexico, Olivia L. Lestrade, Frank Hernandez

Gulf and Caribbean Research

Holopelagic Sargassum is a critical nursery habitat for the early life stages of many marine fishes, including several federally managed species in the United States and Caribbean. Sargassum is often aggregated along surface convergence features where microdebris (synthetic, semi—synthetic, and naturally—derived particles size) have also been found in relatively high concentrations. In this study, we collected microdebris from Sargassum and adjacent open water habitats (in 2018), and juvenile fishes from Sargassum (in 2017 and 2018) in the northern Gulf of Mexico to quantify habitat—specific microdebris concentrations and the degree to which Sargassum—associated juvenile fishes ingest microdebris. Microdebris concentrations within …

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Warm Land Surface Temperatures And Eastern Asian Homo, Robert Patalano, Hong Yang Jan 2023

Warm Land Surface Temperatures And Eastern Asian Homo, Robert Patalano, Hong Yang

Biological and Biomedical Sciences Department Faculty Journal Articles

Climate change and hominin evolution are inextricably linked. Pleistocene climate variability, for example, is thought to have had major influences on hominin morphology, brain size, and diversity. However, clear cause-and-effect relationships between specific climatic events and major evolutionary occurrences are difficult to establish due to temporal and spatial gaps in paleoclimatic, paleoenvironmental, and archaeological records. A new branched GDGT paleotemperature record from the Lantian Basin of Central China (Lu et al., 2022), a location known for the earliest hominin presence in East Asia, illustrates warm land surface temperatures over a two-million-year period between 2.6 and 0.6 Ma, a critical time …

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Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips Jan 2023

Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips

Biological Sciences Faculty Research and Publications

One mechanism proposed to explain high species diversity in tropical systems is strong negative conspecific density dependence (CDD), which reduces recruitment of juveniles in proximity to conspecific adult plants. Although evidence shows that plant-specific soil pathogens can drive negative CDD, trees also form key mutualisms with mycorrhizal fungi, which may counteract these effects. Across 43 large-scale forest plots worldwide, we tested whether ectomycorrhizal tree species exhibit weaker negative CDD than arbuscular mycorrhizal tree species. We further tested for conmycorrhizal density dependence (CMDD) to test for benefit from shared mutualists. We found that the strength of CDD varies systematically with mycorrhizal …

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The Role Of Herbal Medicines In Central American Indigenous Communities; Improving Accessibility To Type 2 Diabetes Mellitus Treatment, Sarai Ortega Jan 2023

The Role Of Herbal Medicines In Central American Indigenous Communities; Improving Accessibility To Type 2 Diabetes Mellitus Treatment, Sarai Ortega

CMC Senior Theses

Indigenous communities in Central America have used various plant medications to treat illnesses and diseases for centuries. Clinicians must consider improving traditional healthcare methods that are intrinsic to indigenous communities in order to increase access to effective treatments, particularly for diseases that these communities are more at risk for. This work aims to propose a method to identify whether traditional preparations of five plants (Anacardium occidentale L., Hamelia patens Jacq., Momordica charantia L, Neurolaena lobota L. R. Br. Ex Cass., and Tecoma stans L. juss. ex Kunth) can reduce blood glucose levels in rats with type two diabetes. Additionally, …

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Movement As Medicine: Dance/Movement Therapy For Individuals With Autism, Parkinson’S Disease, And Cancer, Alessia Zanobini Jan 2023

Movement As Medicine: Dance/Movement Therapy For Individuals With Autism, Parkinson’S Disease, And Cancer, Alessia Zanobini

CMC Senior Theses

Dance/movement therapy (D/MT) is the psychotherapeutic use of expressive, creative movement to support holistic well-being. D/MT views the human being as a single body-mind unit and movement as a manifestation of life experiences. While typically practiced as a mental health intervention, D/MT can be adapted for a variety of populations. This thesis evaluates scientific data for the non-traditional use of D/MT for three conditions: autism, Parkinson’s disease, and cancer. For individuals on the autism spectrum, D/MT can strengthen attunement skills, provide creative communication outlets, and relieve harmful physical manifestations of autism. For individuals with Parkinson’s disease, D/MT can simultaneously ease …

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Effects Of Male Attractiveness On Prostaglandin E2 Production Levels, Kyra Cousins Jan 2023

Effects Of Male Attractiveness On Prostaglandin E2 Production Levels, Kyra Cousins

CMC Senior Theses

Sexual conflict or sexual antagonism can encourage co-evolution between males and females of a given species. In house crickets (Acheta domesticus), unattractive (smaller) and attractive (larger) males seem to use differential allocation of prostaglandin E2 in their spermatophore to pursue different propagation strategies in influencing female oviposition. This study aims to determine the means by which attractive and less attractive males press their reproductive advantage by focusing on prostaglandin E2. To test the hypothesis that the difference in prostaglandin E2 concentration is essential for female reproductive manipulation, spermatophores were retrieved from 21 pairs of mated …

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Admixpipe V3: Facilitating Population Structure Delimitation From Snp Data, Steven M. Mussmann, Marlis R. Douglas, Tyler Chafin, Michael E. Douglas Jan 2023

Admixpipe V3: Facilitating Population Structure Delimitation From Snp Data, Steven M. Mussmann, Marlis R. Douglas, Tyler Chafin, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Quantifying genetic clusters (=populations) from genotypic data is a fundamental, but non-trivial task for population geneticists that is compounded by: hierarchical population structure, diverse analytical methods, and complex software dependencies. AdmixPipe v3 ameliorates many of these issues in a single bioinformatic pipeline that facilitates all facets of population structure analysis by integrating outputs generated by several popular packages (i.e. CLUMPAK, EvalAdmix). The pipeline interfaces disparate software packages to parse Admixture outputs and conduct EvalAdmix analyses in the context of multimodal population structure results identified by CLUMPAK. We further streamline these tasks by packaging AdmixPipe v3 within a Docker container to …

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Evolutionary History And Adaptation To Salinity In American Alligators, John Konvalina Jan 2023

Evolutionary History And Adaptation To Salinity In American Alligators, John Konvalina

Electronic Theses and Dissertations, 2020-2023

Stressful environments can commonly be found at the edge of a species range and may be a driver for adaption in suboptimal environments. Furthermore, the edge of a species' range can expand and contract over time, resulting in multiple independent invasions of the same stressful habitat. Elucidating population genetic structure and demographic history can aid in determining the which geologic factors impact range distributions and when climatic changes occurred driving genetic patterns observed in contemporary populations. Moreover, populations at the edge of the species range may adapt to the stressful environments that occur at the range edge and exhibit genetic …

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Peripheral Clock Disruption And Metabolic Disease: Moving Beyond The Anatomy To A Functional Approach, Gabriella M. Marino, Deanna M. Arble Jan 2023

Peripheral Clock Disruption And Metabolic Disease: Moving Beyond The Anatomy To A Functional Approach, Gabriella M. Marino, Deanna M. Arble

Biological Sciences Faculty Research and Publications

Sleep and circadian disruption are associated with an increased risk of metabolic disease, including obesity and diabetes. Mounting evidence indicates that misaligned and/or non-functional clock proteins in peripheral tissues critically contribute to the presentation of metabolic disease. Many of the foundational studies which led to this conclusion have focused on specific tissues such as the adipose, pancreas, muscle, and liver. While these studies have greatly advanced the field, the use of anatomical markers to manipulate tissue-specific molecular clocks may not be representative of the circadian disruption that occurs within the clinical population. In this manuscript, we argue that investigators can …

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Tyramine And Its Amtyr1 Receptor Modulate Attention In Honey Bees (Apis Mellifera), Joseph S. Latshaw, Reece E. Mazade, Mary Peterson, Julie A. Mustard, Irina Sinakevitch, Lothar Wissler, Xiaojiao Guo, Chelsea N. Cook, Hong Lei, Jürgen Gadau, Brian Smith Jan 2023

Tyramine And Its Amtyr1 Receptor Modulate Attention In Honey Bees (Apis Mellifera), Joseph S. Latshaw, Reece E. Mazade, Mary Peterson, Julie A. Mustard, Irina Sinakevitch, Lothar Wissler, Xiaojiao Guo, Chelsea N. Cook, Hong Lei, Jürgen Gadau, Brian Smith

Biological Sciences Faculty Research and Publications

Animals must learn to ignore stimuli that are irrelevant to survival and attend to ones that enhance survival. When a stimulus regularly fails to be associated with an important consequence, subsequent excitatory learning about that stimulus can be delayed, which is a form of nonassociative conditioning called ‘latent inhibition’. Honey bees show latent inhibition toward an odor they have experienced without association with food reinforcement. Moreover, individual honey bees from the same colony differ in the degree to which they show latent inhibition, and these individual differences have a genetic basis. To investigate the mechanisms that underly individual differences in …

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Lin-35 Is Necessary In Both The Soma And Germline For Preserving Fertility In Caenorhabditis Elegans Under Moderate Temperature Stress, Brian P. Mikeworth, Frances V. Compere, Lisa N. Petrella Jan 2023

Lin-35 Is Necessary In Both The Soma And Germline For Preserving Fertility In Caenorhabditis Elegans Under Moderate Temperature Stress, Brian P. Mikeworth, Frances V. Compere, Lisa N. Petrella

Biological Sciences Faculty Research and Publications

Maintenance of germline function under stress conditions is crucial for species survival. The germ line in many species is especially sensitive to elevated temperature. We have investigated the role of the pocket protein LIN-35 in preserving fertility in Caenorhabditis elegans under moderate temperature stress. We show that lin-35 mutants display several temperature sensitive germline defects, and more severe reductions in brood size at elevated temperatures compared to wild type. This loss of fertility under temperature stress is primarily due to loss of zygotic, but not maternal, LIN-35. Additionally, we have found that expression of LIN-35 is necessary in both the …

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Navigating Rice Seedling Cold Resilience: Qtl Mapping In Two Inbred Line Populations And The Search For Genes, Michael Schläppi, Avery R. Jessel, Aaron K. Jackson, Huy Phan, Melissa H. Jia, Jeremy D. Edwards, Georgia C. Eizenga Jan 2023

Navigating Rice Seedling Cold Resilience: Qtl Mapping In Two Inbred Line Populations And The Search For Genes, Michael Schläppi, Avery R. Jessel, Aaron K. Jackson, Huy Phan, Melissa H. Jia, Jeremy D. Edwards, Georgia C. Eizenga

Biological Sciences Faculty Research and Publications

Due to global climate change resulting in extreme temperature fluctuations, it becomes increasingly necessary to explore the natural genetic variation in model crops such as rice to facilitate the breeding of climate-resilient cultivars. To uncover genomic regions in rice involved in managing cold stress tolerance responses and to identify associated cold tolerance genes, two inbred line populations developed from crosses between cold-tolerant and cold-sensitive parents were used for quantitative trait locus (QTL) mapping of two traits: degree of membrane damage after 1 week of cold exposure quantified as percent electrolyte leakage (EL) and percent low-temperature seedling survivability (LTSS) after 1 …

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Mechanical Devices For Census And Detection Of Off-Host Larval Ticks (Acari: Ixodidae), Donald B. Thomas, Holly D. Gaff, Brenda Leal-Galvan Jan 2023

Mechanical Devices For Census And Detection Of Off-Host Larval Ticks (Acari: Ixodidae), Donald B. Thomas, Holly D. Gaff, Brenda Leal-Galvan

Biological Sciences Faculty Publications

Standard off-host tick census techniques are generally inefficient for larval stages. Also, such techniques expose the investigator to environmental hazards including tick bites. Mechanical devices are an option which were tested under field conditions for proof of concept and in side-by-side comparison to the standard techniques. The tick-vac collected more ticks than the legging technique, but with lower frequency of positive detections. It may have utility for sampling small areas, such as weedy corrals suspected of harboring ticks. Different configurations of tick-bots, robotic vehicles with flags, depended on intensive operator control to avoid obstacles, limiting operation to line of sight. …

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The Coral Bleaching Automated Stress System (Cbass): A Low Cost, Portable System For Standardized Empirical Assessments Of Coral Thermal Limits, Nicolas R. Evensen, Katherine E. Parker, Thomas A. Oliver, Stephen R. Palumbi, Cheryl A. Logan, James S. Ryan, Courtney N. Klepac, Gabriela Perna, Mark E. Warner, Christian R. Voolstra, Daniel J. Barshis Jan 2023

The Coral Bleaching Automated Stress System (Cbass): A Low Cost, Portable System For Standardized Empirical Assessments Of Coral Thermal Limits, Nicolas R. Evensen, Katherine E. Parker, Thomas A. Oliver, Stephen R. Palumbi, Cheryl A. Logan, James S. Ryan, Courtney N. Klepac, Gabriela Perna, Mark E. Warner, Christian R. Voolstra, Daniel J. Barshis

Biological Sciences Faculty Publications

Ocean warming is increasingly affecting marine ecosystems across the globe. Reef-building corals are particularly affected by warming, with mass bleaching events increasing in frequency and leading to widespread coral mortality. Yet, some corals can resist or recover from bleaching better than others. Such variability in thermal resilience could be critical to reef persistence; however, the scientific community lacks standardized diagnostic approaches to rapidly and comparatively assess coral thermal vulnerability prior to bleaching events. We present the Coral Bleaching Automated Stress System (CBASS) as a low-cost, open-source, field-portable experimental system for rapid empirical assessment of coral thermal thresholds using standardized temperature …

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