Digital Commons Network^™

Description Of Neoperla Philippinensis Sp. Nov. Confirmed Using Integrative Taxonomy And Some Notes On Neoperla Oculata Banks, 1924 (Plecoptera: Perlidae), Arthien Lovell P. Pelingen

Biology Faculty Publications

I re-examine the assumed phenotypic variable Neoperla oculata Banks, 1924 (Plecoptera, Perlidae) and describe Neoperla philippinensis sp. nov. All material examined herein are collections from the Mindanao region, of which supplementary information were provided including COI mtDNA accessions, female adult associations, and egg morphology characterization.

Incorporating Sex-Diverse And Gender-Inclusive Perspectives In Higher Education Biology Courses, Breanne N. Harris, A Kelsey Lewis, Sam L. Sharpe, Teri J. Orr, Christopher T. Martine, Chloe C. Josefson

Faculty Journal Articles

Inclusive teaching is teaching in a way that reaches all students in the classroom; this is beneficial for everyone, particularly for those with minoritized identities. Instructors play a critical role in scaffolding how students are exposed to and learn science content in the classroom. In this manuscript, we discuss how biology instructors can make their classrooms more inclusive with regard to sex and gender diversity content. Many topics in biology are based on androcentric, heteronormative, and oppressive framing, even though those lenses are more reflective of our own history and culture than they are of the diversity we see in …

Carbaryl-Induced Leaf Necrosis In Vitis Rupestris B-38, Courtney Nicole Duncan

Graduate Theses/Dissertations

Agricultural insecticides are formulated to target insects while minimizing harm to the intended crop. In rare instances, however, insecticides induce harmful physiological reactions in certain plant genomes, inflicting severe tissue damage. This project investigated the genetic basis of such a reaction observed in the grape genotype Vitis rupestris B38 following exposure to the insecticide carbaryl, which manifests as interveinal leaf necrosis. Through analysis of an F1 hybrid progeny of this grapevine, I mapped this phenotype to a QTL on chromosome 16. The carbaryl-sensitive trait was repeatedly mapped to the same locus using phenotype data from two different field locations and …

Characterization Of The Interaction Of Three Domains Of Nlrp2 (Pyd, Lrr, And Nacht) With Ebp1 Protein, Rakshya Bhatta

Graduate Theses/Dissertations

NOD-like receptors (NLRs) are cytoplasmic proteins essential for various immune responses such as reactions to infectious diseases, metabolic and cellular damage, fetal development, and cancer. Among the 22 identified NLR proteins, research has highlighted the significant roles of NLRP2 in fetal development. NLRP2 is also classified as a maternal effect gene and the mutation to this gene can lead to DNA methylation imprinting defects, altered gene expression, and conditions such as recurrent miscarriages (RMs). The protein EBP1, which has been found to have roles in embryonic development has been implicated in the regulation of DNA methylation. Recent studies have also …

Disturbance-Prone Ozark Streams: Interactions Between Plants And The Stream Environment, Alexis Renea Reifsteck

Graduate Theses/Dissertations

Ozark streams are extremely diverse ecosystems, despite frequent flood disturbance. Plant communities are frequently altered by bed-moving floods but are extremely resilient. We know that land-use, sediments, and nutrients can impact plant communities, but more can be divulged about how plants alter communities and how environmental variables influence plant community composition in disturbance-prone Ozark streams. To address this, I examined two key aspects of Ozark stream ecosystems: the role of American Water Willow, Justicia americana, in structuring aquatic habitats and the factors influencing gravel bar plant community composition. In the first chapter, I investigated how J. americana, a …

Effects Of Subchronic Oral Pfos Exposure Via Drinking Water On Gut Microbiota Composition, Emma Ann Loder

Graduate Theses/Dissertations

Perfluorooctanesulfonic acid (PFOS) has been phased out of production in the United States, but because of its resistance to degradation and its persistence in the environment, PFOS can be found in drinking water across America. Oral exposure to PFOS has been linked to adverse developmental, immunological, hepatic, and endocrine health. Oral PFOS exposure may alter the gut microbiota and cause dysbiosis, which is implicated in the pathology of inflammatory diseases like Crohn’s disease, diabetes, and ulcerative colitis. The goal of the study was to investigate the effects of subchronic oral PFOS exposure on the composition of the gut microbiota. Adult …

Demographics And Recruitment Patterns In A Reintroduced Population Of Alligator Snapping Turtles (Macrochelys Temminckii), Melisa P. Blasky

Graduate Theses/Dissertations

Reintroductions have increasingly become a useful tool for managing wildlife populations, but post-reintroduction studies to evaluate the success of these efforts are often lacking. In 2007, 265 Alligator Snapping Turtles (Macrochelys temminckii) were released into several semi-impounded wetlands in the Washita River drainage basin in southeastern Oklahoma, where surveys in the late 1990s suggested that the species was functionally extirpated. I conducted turtle trapping surveys using baited hoop nets to assess demographics and recruitment patterns in the population 11–12 years after the initial reintroduction. I estimated the age of juveniles by counting scute annuli and compared the data …

How Environmental Variation Affects Bumblebee Cognition And The Gut Microbiome, Parker Madison Campbell

Graduate Theses/Dissertations

This master's thesis examines the influence of environmental variables on cognitive processes in Bombus impatiens, focusing on the effects of ingested microbial communities on gut microbiota and their subsequent impact on associative color learning. The study implemented controlled feeding trials with isolated microbe species (Candida bombi, Metschnikowia reukaufii, Lactobacillus micheneri and a control with no microbes) to analyze shifts in gut microbial composition in foraging bees. Results indicated minor alterations in the microbial profiles of bees, yet no statistically significant correlation was found between these modifications and variations in learning or memory performance. Furthermore, the study assessed the impact of …

Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya

Selected Full-Text Master Theses 2021-

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key …

Deepwhalenet: A Climate Change-Aware Fft-Based Neural Network For Underwater Passive Acoustic Monitoring, Nicholas Ryan Rasmussen

Dissertations and Theses

In the face of escalating climate threats, the conservation of whale species has become increasingly critical. Traditional acoustic monitoring methods, burdened by extensive pre-processing and post-processing, need more adaptability and efficiency for effective marine mammal surveillance. This study introduces DeepWhaleNet, a novel deep-learning framework tailored for Underwater Passive Acoustic Monitoring (UPAM). DeepWhaleNet is designed to streamline whale detection by directly analyzing raw log-power spectrograms, thus extracting essential acoustic features to conserve these endangered species. The framework employs an extensive short-time Fourier transform (STFT) for input processing and a customized ResNet-18 architecture for classification, distinguishing whale vocalizations from ambient noise and …

Exploring The Role Of Nonmuscle Myosin Ii In Renal Thick Ascending Limb Epithelial Cell Protein Trafficking And Organelle Architecture, Brook Busselman

Dissertations and Theses

The traditional functional roles of the actin associated motor protein nonmuscle myosin II (NM2) have been thoroughly studied. However, the nontraditional roles for NM2 in the context of membrane remodeling and protein trafficking events in cells have just recently been unraveled. Our work has found that genetic inactivation of NM2 protein isoforms MYH9 and MYH10 in the renal epithelium of adult mice leads to progressive kidney disease. Aberrant protein transport and localization in cells of the thick ascending limb (TAL) of the kidney nephron is accompanied by upregulation of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) pathway. …

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …

Do The Sexually Dimorphic Biofluorescent Glands Of Plethodon Cinereus Function In Mate Choice And Are They Seasonally Regulated By Photoperiod?, Sam C. Moody-Wynn

Masters Theses

The novel biofluorescent glands of the Eastern Red-backed Salamander (Plethodon cinereus), have been shown to be sexually dimorphic where males have larger and greater numbers of glands than females. Interestingly, a seasonal pattern of increased numbers of fluorescent glands, which mirrors the peak in testosterone levels, has been described. Currently, the function and regulation of the fluorescent glands is unknown. I predicted that the glands function as a form of male ornamentation used in intersexual communication, and perhaps act as an honest signal of male quality. Even though the fluorescent glands are located on the underside of the tail, there …

Effect Of Walleye Fry Stocking On Future Gillnet Cpue, Alexander Barker

Journal of Earth and Life Science

Walleye Sanders vitreus for many states, is a key attractant of anglers from around North America. Due to immense popularity, management efforts have been conducted to maintain Walleye populations, especially in the form of stocking. The purpose of this study is to analyze the effect of Walleye fry stocking on gillnet catch per unit effort (CPUE). A total of 90 lakes were analyzed for this study within three defined regions of Minnesota, 30 from each region. The regions chosen for this study are the Minnesota Department of Natural Resources northern pike Esox lucius regulation regions which consist of the Southern, …

Effects Of Climate Change On Seed Germination May Contribute To Habitat Homogenization In Freshwater Forested Wetlands, Kori Carr, Xavier Ozowara, Taylor M. Sloey

Biological Sciences Faculty Publications

Climate changes in temperate regions are expected to result in warmer, shorter winters in temperate latitudes. These changes may have consequences for germination of plant species that require a period of physiological dormancy. The effect of cold duration on seed germination has been investigated in a number of plant taxa, but has not been well studied in wetland and bottomland forest tree species, an ecosystem that is threatened by habitat homogenization. Our work sought to test the role of changing winter temperatures on seed germination in specialist (Nyssa aquatica and Taxodium distichum) and generalist (Acer rubrum and Liquidambar styraciflua) tree …

Natural Enemies Of Varroa Destructor Identified From Eastern North America Honey Bee Colonies: A Biological Survey Of Candidates For Mite Control From Maryland, Usa, Francisco Posada-Flórez, Daniel Sonenshine, Jay Evans, Dawn Boncristiani, Monica Pava-Ripoll, Steven Cook

Biological Sciences Faculty Publications

A survey was conducted of microbes and arthropods associated with Varroa destructor (Acari: Varroidae) mites (hereafter, Varroa) collected from honey bee colonies in Maryland, USA. Live Varroa were challenged with samples of both microbes and arthropods and assessed for their potential as biocontrol agents against the mites. To our knowledge, this is the first survey of natural enemies of Varroa conducted in North American honey bee colonies. The survey revealed 21 fungi, 25 bacteria, one nematode, and 8 arthropod species associated with Varroa in honey bee colonies, and from challenge assays using 11 of these, including 4 species of …

Lessons From An Iconic Grouper: Sex, Science, And Serendipity, Yvonne J. Sadovy De Mitcheson

Gulf and Caribbean Research

From a common little damselfish and spectacular grouper aggregations, to disappearing reef fishes and the dark side of the international seafood trade, my journey has passed through a fascinating and worrying period of change in the way we view and treat our oceans. Although much of my career has been in the Indo—Pacific, I focus here mainly on my professional ‘roots’ in the Caribbean and on the Nassau Grouper Epinephelus striatus, a species that kept me curious, taught me patience, never to assume and always to stay hopeful. This fish is almost an obsession; why can we not better safeguard …

Feeding Systems And Fresh Lamb Color Stability, Koushik Mondal

Theses and Dissertations--Animal and Food Sciences

Consumers consider the surface color of fresh lamb as the indicator of freshness and wholesomeness, which affects their purchase decisions. The redox chemistry of myoglobin (Mb) determines the color of fresh lamb. Artificial raising on milk replacer (a pre-weaning strategy) as well as red clover supplementation are management strategies used in lamb production. This thesis focuses on the effects of artificial raising on milk replacer and red clover supplementation on the color and oxidative stability of fresh lamb.

In the first experiment, the effect of pre-weaning with milk replacer on the carcass attributes, color stability and lipid oxidation of lamb …

The Morphological Evolution Of The Inner Ear In Lacertoidea (Squamata), Cristian Hernandez

Biology Dissertations - Archive

The popularization of high-resolution computed tomography(HRCT) has produced a revolution in the field of comparative anatomy. This technology allows us to reach a level of morphological detail in our observations that has never been possible previously. Now we can perform digital dissections isolating structures of interest such as individual bones, soft tissue organs, and even generate 3D models of body cavities (endocasts) such as the braincase and inner ear. Encompassed with the progress of the HRCT, 3D geometric morphometrics have emerged as a common tool to address morphological questions in a statistical context that outperforms the traditional 2D geometric morphometrics. …

The Effect Of Body Size On Mate Choice By Ambystoma Laterale Breeding With Unisexual Salamanders, Cole Christian Duvall

Senior Honors Theses and Projects

Within the genus Ambystoma exists a complex of unisexual (all female) polyploid salamanders, which can breed with males of five sexual species of salamander. Unisexuals face intense sexual selection from diploid males, who prefer to mate with females of their own species. At the Edwin S. George Reserve (ESGR), it has been observed that LLJ unisexuals with large body sizes migrate to breeding ponds earlier than small unisexuals. A possible explanation for this behavior is that large LLJ unisexuals are sexually selected against, when compared to small unisexuals. We hypothesized that A. laterale males would prefer smaller LLJ unisexuals, when …

Documenting Plant Biodiversity At The Emu-Owned Parsons Center For Arts And Sciences, Emily Edwards

Master's Theses and Doctoral Dissertations

We present the first vascular plant species checklist for the Eastern Michigan-University-owned property called the Parsons Center for Arts and Sciences. A voucher for each plant species has been deposited in the Eastern Michigan College herbarium and collection data is digitized in the Consortium of Midwest Herbaria. A minimum of three images per species are also available on iNaturalist . The Consortium and iNaturalist databases are publicly available and provide georeferenced locations for each plant. One hundred seventy-one plant species (in 137 genera and 61 families) were documented and identified, representing about 15% of plant species and 41% of families …

Lhx3/4 Initiates A Cardiopharyngeal-Specific Transcriptional Program In Response To Widespread Fgf Signaling, C. J. Pickett, Hannah N. Gruner, Bradley Justin Davidson , '91

Biology Faculty Works

Individual signaling pathways, such as fibroblast growth factors (FGFs), can regulate a plethora of inductive events. According to current paradigms, signal-dependent transcription factors (TFs), such as FGF/MapK-activated Ets family factors, partner with lineage-determining factors to achieve regulatory specificity. However, many aspects of this model have not been rigorously investigated. One key question relates to whether lineage-determining factors dictate lineage-specific responses to inductive signals or facilitate these responses in collaboration with other inputs. We utilize the chordate model Ciona robusta to investigate mechanisms generating lineage-specific induction. Previous studies in C. robusta have shown that cardiopharyngeal progenitor cells are specified through the …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Upa-Mediated Polyamidoamine Dendrimer-Based Targeted Gene Delivery System On Triple-Negative Breast Cancer, Hsin-Yin Chuang

Masters Theses

"Around 15% of breast cancers are triple-negative breast cancer (TNBC), which is characterized by the absence of three common receptors—ER, PR, and HER2, and therefore do not respond to hormonal or anti-HER2 therapies. It is urgent to explore targeted therapeutic strategies for TNBC due to its poor prognosis and rare effective targeted therapy. In this study, we developed a polyamidoamine (PAMAM) dendrimer-based targeted gene delivery system—GDP-uPA, to utilize urokinase-type plasminogen activator (uPA) to target uPA receptor (uPAR), which is highly expressed in both TNBC cells and cancer-associated stromal cells. Our results of 1H NMR spectrum, TEM imaging, and MTT assay …

Protein Turnover Rate And Life History As An Explanation Of The Extreme Difference In Cost Of Biosynthesis In Cockroach Nymphs And Painted Lady Butterfly Larva, Kyara Nicole Holloway

Masters Theses

The cost of biosynthesis in painted lady caterpillars is far lower than that of a red runner cockroach. A promising explanation for this phenomenon is the protein turnover rate for the animal during biosynthesis and tissue maintenance. Previous research has shown that, per gram of body weight, the painted lady butterfly can synthesize a biomass using up to 15x less energy and resources than the cockroach, even under similar conditions. The resulting ratios, especially in respect to growth rate, show a wide disparity between species in such a manner that does support a protein synthesis and degradation rates as a …

A Review Of Dielectric Barrier Discharge Cold Atmospheric Plasma For Surface Sterilization And Decontamination, Kolawole Adesina, Ta Chun Lin, Yue-Wern Huang, Marek Locmelis, Daoru Frank Han

Biological Sciences Faculty Research & Creative Works

Numerous investigations have shown that non-equilibrium discharges at atmospheric pressure, also known as "cold atmospheric plasma" (CAP) are efficient to remove biological contaminants from surfaces of a variety of materials. Recently, CAP has quickly advanced as a technique for microbial cleaning, wound healing, and cancer therapy due to the chemical and biologically active radicals it produces, known collectively as reactive oxygen and nitrogen species (RONS). This article reviews studies pertaining to one of the atmospheric plasma sources known as Dielectric Barrier Discharge (DBD) which has been widely used to treat materials with microbes for sterilization, disinfection, and decontamination purposes. To …

In Vitro Assessment Of The Anti-Biofilm Effectiveness Of Copper And Zinc Enhanced Borate Bioactive Glass Using Processed Microscopic Images, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

The escalating burden of nosocomial infections presents a formidable challenge to healthcare systems worldwide, leading to increased morbidity, prolonged hospital stays, and elevated healthcare costs. These infections are often resistant to conventional antibiotic therapies due to their association with biofilms which contribute to the persistence and resistance of pathogens. In addressing the challenge of biofilm-associated nosocomial infections, borate bioactive glasses (BBGs) have emerged as promising biomaterials. Doping these glasses with antimicrobial metals could potentially harness their antibacterial properties to prevent biofilm formation. This study undertakes a rigorous evaluation of the anti-biofilm efficacy of copper and zinc-doped BBGs by employing processed …

Advanced Detection Of Sars-Cov-2 And Omicron Variants Via Mxene-Graphene Hybrid Biosensors Utilizing Nucleic Acid Probes, Jiaoli Li, Yuwei Zhang, Congjie Wei, Yanxiao Li, Zhekun Peng, Hsin Yin Chuang, Logan Pearce, Adrianus Boon, Yue-Wern Huang, Dong Hyun Kim, Risheng Wang, Chenglin Wu

Biological Sciences Faculty Research & Creative Works

Low-cost biosensors that can rapidly and widely detect viruses are critical for faster diagnosis and treatment decision-making, especially for infections. The commonly used field-effect transistor is sensitive to the biomarker's detection but struggles with precise detection, particularly of nontargets such as ions and proteins. To overcome this limitation, we developed a field-effect transistor biosensor design based on MXene-graphene materials to increase the accuracy and sensitivity of virus detection. Based on the hybridization process between two complementary DNA strands, single-stranded nucleic acids were immobilized on the sensing surface via 3-aminopropyltriethoxysilane and glutaraldehyde to capture the nucleic acids of the target virus. …

Dimeric Immunoglobulin A As A Novel Diagnostic Marker Of Measles Infection, Khayriyyah Mohd Hanafiah, Joanne Hiebert, Vanessa Zubach, Alberto Severini, David A. Anderson, Heidi E. Drummer

Biology Faculty/Staff Publications

Despite tremendous measles incidence reduction through universal vaccination, elimination efforts rely on improved surveillance. The detection of anti-measles immunoglobulin M (IgM) by enzyme-linked immunosorbent assay is the standard laboratory diagnostic method. However, true infection is rare, and seroconversion following measles, mumps, and rubella vaccination also generates IgM, which results in low positive predictive values of assays in elimination settings, thus necessitating confirmatory testing. Improved diagnostic tests for measles infection are a World Health Organization research priority. We investigated whether dimeric immunoglobulin A (dIgA), the predominant antibody produced in mucosal immunity, may be a marker of recent or acute measles infection. …

2024 Recipient's Application, Kasey Christopher

Open Education Teaching Award

No abstract provided.