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Effects Of Fertilization On Water Quality In Grayson Lake, Kentucky., Albert W. Remley Ii

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Albert W. Remley II on January 15, 1998.

Construction And Analysis Of Mutant Hsv-1 Viruses Having Mutations In The Yy1 And/Or Sp1 Binding Sites Of The Glycoprotein D (Gd) Gene Promoter, Sabine Ludwig

Dissertations and Theses

The purpose of this study was to construct and analyze four recombinant herpes simplex virus type 1 (HSV-1) viruses having mutations in the YY1 and/or Sp1 binding sites of the glycoprotein D (gD) gene promoter and a control virus containing a wild type (wt) gD promoter. The YY1 and Sp1 binding sites in the HSV-1-gD gene promoter have been shown in earlier in vitro experiments to be essential for virus-induced gD gene expression with Sp1 playing the major role. The first part of my project required the construction of four different plasmids containing a HSV-1 sequence that included the gD …

Differences In Lean And Obese Skeletal Muscle Myoblasts Of Adult And Post-Natal La/N (Faf) Rats: A Confocal Description Of The Skeletal Muscle Myoblast, J. Adam Noah

Theses, Dissertations and Capstones

Mutant strains of obese rats are currently being studied to reveal the mechanisms of increased lipid synthesis and decreased lipid oxidation in these rats. It was originally thought that much of the fat production that leads to obesity is the result of metabolic processes in the liver and adipose tissue. Studies by Kahle et al.³ showed that the liver and adipose tissue account for only 22% and 7% respectively, of total fatty acid synthesis. It was also shown that 21 % of total de novo fatty acid was localized in the postural skeletal muscle.

The dry weight of the …

Sidescan Sonar Image, Surficial Geological Interpretation, And Bathymetry Of The Long Island Sound Sea Floor Off Milford, Connecticut, Roman Zajac, David C. Twichell, Larry J. Poppe, Ralph S. Lewis, Veeann Cross

Biology and Environmental Science Faculty Publications

The surficial geology of a 6-km X 8-km section of the western part of Long Island Sound has been mapped. The map area is 4 to 12 km south of the mouth of the Housatonic River in 14 to 40 m water depth (see location map). This study is part of an interdisciplinary program to define the surficial geology and benthic habitats in Long Island Sound and to see how they are changing with time, and includes, in addition to this map area, the other survey areas shown on the location map. The U.S. Geological Survey, in cooperation with the …

Land Use Patterns In Relation To Lake Water Quality In The Great Pond Watershed, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: Great Pond (2012, 2010, 1998)

Lakes are natural resources, which have many effects on the land surrounding them, They support adjacent communities by providing water and regulating temperatures, helping to define the surrounding ecosystem, and serving as sources of drinking water as well as recreation. The prolonged presence of human activity in a watershed can disturb the physical and chemical cycles of the lake and its surrounding ecosystems (Henderson-Sellers and Markland 1987). Over time, lakes undergo a process called eutrophication, a natural aging process during which the nutrient levels increase and dissolved oxygen levels decrease (Smith and Smith 1998). As the lake ages or becomes …

Pal-Like Protein Present In Chromatium Vinosum, H. Benan Dincturk, David B. Knaff

Turkish Journal of Biology

PAL is a peptidoglycan-associated lipoprotein found in the cell membranes of many Gram-negative bacteria. The presence of PAL has been demonstrated in Escherichia coli and many bacteria. In this paper, we show the presence of a gene coding for a PAL-like protein in C. vinosum, a photosynthetic bacteria and the partial sequence of the PAL gene.

The Role Of Protein Synthesis On The Adhesionstrength Of Chl Cells, Ali Yildirim, William Jd Whish

Turkish Journal of Biology

Cells require protein synthesis to perform the adhesion function properly. In order to dis-cover the effect of endogenous protein synthesis on CHL cell adhesion; the adhesion strengths of cul-tured Chinese Hamster Lung (CHL) cells that were grown for 24 hours on the glass surface in the pres-ence of 1µgml -1 of cycloheximide or emetine or, both were measured quantitatively using a converg-ing laminar flow chamber. The inhibition of protein synthesis by above drug(s) reduced cell adhesion strength significantly. Hence critical shear stress of detachment of cell were 8.68±1.52 Nm -2 , 8.13±1.48Nm -2 , and 6.57±1.01Nm -2 , respectively. While …

Recent Developments In Molecular Genetics Of Plantdisease Resistance, Hüseyin Basim

Turkish Journal of Biology

The field of plant-microbe interactions has seen several recent break-throughs as cloning and characterization of resistance ( R) genes from plants and avirulence ( avr) genes from pathogens. Although genetics, histology, and biochemistry of plant disease resistance are well known at the cellu-lar and population levels, a full understanding of pathogen resistance in plants at molecular level is recently becoming feasible throught cloning and sequencing of several putatively interacting plant and pathogen genes. This knowledge may lead to the establishment of important strategies in plant dis-ease control. The objective of this review is to provide a short and selective overview …

Correlations Between Changes In The Amount Ofendogenous Phytohormones And Flowering In Thesafflower ( Carthamus Tinctoriusl.), Hasan Baydar, Salih Ülger

Turkish Journal of Biology

Changes in the amounts of endogenous gibberellic acid (GA 3 ), indole-3-acedic acid (IAA) and abscisic acid (ABA) during different growth periods of the safflower were studied by high-perfor-mance liquid chromatography (HPLC). A correlation was confirmed between low levels of GA 3 , as well as high levels of ABA, and the initia-tion of flowering. IAA reached its maximum level during bud formation, indicating that IAA might play an active role in the differentiation of bud formation. Changes in the amount of endogenous phytohormones in flower buds located in different parts of the plant indicate that ABA in particular plays …

A Study On The Phytoplankton Of Hasan Uğurludam Lake (Samsun-Turkey), Arif Gönülol, Olcay Obali

Turkish Journal of Biology

The composition and seasonal variations of phytoplankton of Hasan UGURLU Dam Lake were studied using samples collected from two stations between July 1992 to December 1993. 57 taxa were identified belonging to the Bacillariophyta, Chlorophyta, Cyanophyta, Dinophyta and Euglenophyta divisions of phytoplankton. In the phytoplankton, Asterionella formosa, Cyclotella planc-tonica, Pediastrum simplex and Ceratium hirundinella caused water blooms in certain months. The light density and temperature affected the composition and seasonal variations in phytoplankton considerably. Nutrient levels in the lake did not limit the seasonal variations in phytoplankton. It was determined that Lake Hasan Ugurlu had mesotrophic characteristics because of the …

Contribution To The Geographic Distribution Ofrodent Species And Ecological Analyses Of Theirhabitats In Asiatic Turkey, Nuri Yi̇ği̇t, Ercüment Çolak

Turkish Journal of Biology

Rodent species in 17 selected localities were determined, and these localities were then compared with respect to rodent species composition. It was determined that a total of 27 rodent species are found in these localities, the number in each locality ranging from 6 to 12. Species previ-ously recorded in mixed and decidous forests in northern Asiatic Turkey, such as Glis glis, Muscardinus avellanarius, Microtus subterraneus and Clethrionomys glareolus, were found not to range across forested localities in west and south Asiatic Turkey in the dry summer season. It was also determined that rodents, which live in similar habitats in different …

Decolorization Of Orange Ii Dye With The Crudeculture Filtrate Of White Rot Fungus, Coriolusversi-Color, Özfer Yeşi̇lada, Birgül Özcan

Turkish Journal of Biology

Decolorization of Orange II dye was demonstrated with crude culture filtrate of white rot fungus, Coriolus versicolor. It was determined that the rate and extent of decolorization were depend-ent on the age (growth phase) of the crude culture filtrate (CCF). Dye decolorization abilities of the CCF of Funalia trogii, Pleurotus sajor-caju and Phanerochaete chrysosporium ME446, after incubation for 12 days, were also studied. Phanerochaete chrysosporium ME446 was observed to be unable to decolorize any of 4 dyes used. Cyanide and azide, which are known inhibitors of enzyme, inhibited dye decolorization ability of the CCF. Heat treatment terminated decolorization activity of …

Biodegradation Of Heptachlor By Phanerochaetechrysosporium Me 446: The Toxic Effects Ofheptachlor And Its Metabolites On Mice, Münevver Arisoy, Nazif Kolankaya

Turkish Journal of Biology

On the basis of degradation properties of Phanerochaete chroysosporiumME 446 found in white-rot fungi in respect of many organic compounds with toxic, mutagenic and carcinogenic charac-teristics, the physiological conditions effective for the degradation of heptachlor (1, 4, 5, 6, 7, 8, 8- heptachloro-3a, 4, 7, 7a-tetrahydro-4, 7-methanoindene), an organic chlorine used in insecticides Phanerochaete chrysosporium ME were determined. The potential toxic effects of heptachlor and metabolites on mice were also investigated. For the degradation of heptachlor, the optimum pH was found to be 5, incubation period 20 days, and incubation temperature, 30 o C-35 o C, while the optimum concentrations …

Natural And Genetic Engineering Of The Heat-Shock Protein Hsp70 In Drosophila Melanogaster: Consequences For Thermotolerance, Martin E. Feder, Robert A. Krebs

Biological, Geological, and Environmental Faculty Publications

Larvae of the fruit fly, Drosophila melanogaster, live within necrotic fruit, a challenging environment in which larvae can experience severe thermal stress. One response to thermal stress, the expression of heat-shock proteins (Hsps), has evolved distinctively in this species; the gene encoding Hsp70 has undergone extensive duplication and accounts for the bulk of Hsps that are expressed upon heat shock. Genetic engineering of hsp70 copy number is sufficient to affect thermotolerance at some (but not all) life stages. Increases in Hsp70, moreover, can protect intact larvae against thermal inactivation of the enzyme alcohol dehydrogenase and thermal inhibition of feeding. …

Simulation Of Complex Movements Using Artificial Neural Networks, Holk Cruse, Jeffrey Dean, Thomas Kindermann, Josef Schmitz, Michael Schumm

Biological, Geological, and Environmental Faculty Publications

A simulated network for controlling a six-legged, insect-like walking system is proposed. The network contains internal recurrent connections, but important recurrent connections utilize the loop through the environment. This approach leads to a subnet for controlling the three joints of a leg during its swing which is arguably the simplest possible solution. The task for the stance subnet appears more difficult because the movements of a larger and varying number of joints (9-18: Three for each leg in stance) have to be controlled such that each leg contributes efficiently to support and propulsion and legs do not work at cross …

Effects Of Cutting Ashe Juniper Woodlands On Small Mammal Populations In The Texas Hill Country, K. A. Schnepf, J. A. Heselmeyer, David O. Ribble

Biology Faculty Research

We studied the effects of cutting Ashe juniper (Juniperus ashei Bucholz) woodlands on populations of small mammals at Friedrich Wilderness Park, north of San Antonio, Texas. Three patches of juniper ranging from 1.8 ha to 2.4 ha were cut to provide habitat for endangered black-capped vireos (Vireo atricapillus Woodhouse). We trapped small mammals along transects placed in the treated patches and in untreated areas of the park from October 1995 to May 1996 and again from October 1996 to March 1997. Three species of small mammals were trapped, but Peromyscus pectoralis Osgood (white-ankled mouse) was the …

Ecology And Natural History Of The Cow Knob Salamander, Plethodon Punctatus Highton, In West Virginia, Robert B. Tucker

Theses, Dissertations and Capstones

Very little has been written on the ecology and natural history of the Cow Knob salamander, Plethodon punctatus Highton, since it was described by Highton (1971). Plethodon punctatus is known only from the higher elevations of Great North (above 2,800 ft) and Shenandoah (above 3,000 ft.) Mountains in Virginia and West Virginia (Highton, 1972; Conant and Collins, 1991). The type locality is Cow Knob, Pendleton County, West Virginia. Plethodon punctatus is a large (up to 75 mm snout-to-vent length) dorso-ventrally flattened salamander with creamish iridiophore spots on the back and sides, webbed feet for a fossorial lifestyle, and large …

Life History, Ecology, And Low Ph Tolerance Of The Freshwater Prawn, Palaemonetes Kadiakensis Rathbun, In A Mitigated Wetland Of The Green Bottom Wildlife Management Area, West Virginia, Jennifer Ann Aderman

Theses, Dissertations and Capstones

An ecological life history study of the freshwater prawn Palaemonetes kadiakensis was conducted at the mitigated wetland of the Green Bottom Wildlife management Area (GBWMA). Monthly samples were collected from November 1996 through November 1997. In addition, weekly samples were collected from May through November for growth and reproductive analysis. Monthly samples yielded 520 prawns. Palaemonetes kadiakensis had a one-year life cycle, with all adults dying by September. Males averaged 23.82 mm (range = 14.15 to 36.75 mm) and females averaged 26.78mm (range = 15.06 to 38.60 mm) in length. Males averaged 0.087g (range = 0.014to 0.293 g) and females …

Molecular Mapping Of The Fatty (F) (Formerly Corpulent) Gene In The La/N Rat Obesity Model, Kenneth G. Butz

Theses, Dissertations and Capstones

The LA/N-corpulent (cp) rat obesity model was a congenic rat strain developed at the National Institutes of Health by C. T. Hansen . The LA/N-cp rat originated from breeding the Koletsky rat, possessing the corpulent gene, with the normotensive LA/N inbred rat. The autosomal recessive single gene mutation, cp, produces obesity with hyperphagia, hyperinsulinemia and elevated activities of liver and skeletal muscle lipogenic enzymes in the LA/N-cp rat model. The Zucker-fatty mutation, which was mapped to rat chromosome , resulted in a comparable, but not identical obesity syndrome. Breeding experiments by Yen et al …

Regenerative Biology: New Tissues For Old, David L. Stocum

Journal of the Minnesota Academy of Science

Throughout the human life cycle, tissues are regenerated either continuously to maintain tissue integrity in the face of normal cell turnover or in response to acute or chronic damage due to trauma or disease states. Blood, epithelia of skin and tubular organs, hair and nails, and bone marrow are examples of human tissues which regenerate continuously as well as in response to damage. Bone, muscle, adrenal cortex and kidney epithelium also regenerate in response to damage, and bone is continually remodeled in response to stress vectors.

The response of many other vital tissues to damage, however, is not regeneration but …

Perspectives On Liver Regeneration, Janeen H. Trembley, Clifford J. Steer

Journal of the Minnesota Academy of Science

No abstract provided.

Tissue Engineering: At The Interface Between Engineering, Biology And Medicine, Brenda Ogle, Priti Gairola, Jodi Balik, Daniel L. Mooradian

Journal of the Minnesota Academy of Science

No abstract provided.

Bacterial Analysis Of Refrigerated Human Milk Following Infant Feeding, Rachel Renee Brusseau

CUP Undergraduate Research

The number of infants who are breastfed is on the rise, as is the number of women in the workforce. Many women who choose breastfeeding after returning to work, express milk during the day and store this milk for a future feeding. When infants do not finish a bottle of expressed breastmilk, doctors recommend unfinished portions be thrown away. This study examined bacterial levels in expressed, partially consumed breastmilk that was stored for 48 hours at 4-6° C. A portion of unconsumed milk was examined as a control. Samples were taken every 12 hours for bacterial analysis. Tests were performed …

Analyzing Seed Production And Germination In Pityopsis Ruthii, Jennifer M. Park

Capstone Research Projects

No abstract provided.

A Qualitative Analysis Of Larval Drosophila Melanogaster Mitochondrial Dna Via The Polymerase Chain Reaction, Jason Gatling

Capstone Research Projects

No abstract provided.

The Adhesion Of Streptococcus Sa/Ivarius And Staphylococcus Aureus To Five Dental Composite Resins, Arturo James Lopez

Capstone Research Projects

Resin composites are used for anterior esthetic restorative procedures. Breakdown areas between cavity preparations and restorative materials can provide potential sites of reinfection. Reducing the marginal breakdowns by using effective composite resins is important to reduce the amount of recurrent caries.

Each composite type was analyzed for bacterial adherence after bacterial exposure by microscopically counting them after staining. The purpose of this experiment was to measure and observe the ability of Streptococcus sa/ivarius and Staphylococcus aureus to adhere to five different resin composites (APH, Charisma, Herculite, Silux, and Z-100) using an in vitro assay.

It was found that there is …

Reproductive Demographics And Early Life History Of The Shovelnose Sturgeon (Scaphirhynchus Platorynchus), Robert E. Colombo

Faculty Research & Creative Activity

Due to the collapse of the historic caviar fisheries in the Volga River and Caspian Sea, the demand on domestically produced caviar has increased. To supply the increased demand, the harvest of the shovelnose sturgeon Scaphirhynchus latorynchus has increased. Because caviar fisheries preferentially harvest females, information regardingthe sexual demographics of these populations is essential for effective fisheries management. To date, knowledge regarding the sex ratios and reproductive development of the shovelnose sturgeon population in the Middle Mississippi River is lacking. In Chapter 1, I describe this problem in detail and in subsequent chapters I explain how I addressed this problem …

Reproductive Demographics And Early Life History Of The Shovelnose Sturgeon (Scaphirhynchus Platorynchus), Robert Colombo

Faculty Research & Creative Activity

Due to the collapse of the historic caviar fisheries in the Volga River and Caspian Sea, the demand on domestically produced caviar has increased. To supply the increased demand, the harvest of the shovelnose sturgeon Scaphirhynchus latorynchus has increased. Because caviar fisheries preferentially harvest females, information regardingthe sexual demographics of these populations is essential for effective fisheries management. To date, knowledge regarding the sex ratios and reproductive development of the shovelnose sturgeon population in the Middle Mississippi River is lacking. In Chapter 1, I describe this problem in detail and in subsequent chapters I explain how I addressed this problem …

The Use Of Emergent Rocks As Refugia For The Cheat Mountain Salamander, Plethodon Nettingi Green, Beth Anne Pauley

Theses, Dissertations and Capstones

Plethodon nettinqi was listed as a threatened species in 1989 by the United States Fish and Wildlife Service (Pauley, 1991). Its total range is within 5 counties in eastern West Virginia. There are fewer then 60 disjunct populations known and most populations are above 3,000 ft. and are associated with emergent rocks or narrow ravines with Rhododendron. It is hypothesized the P. netting! survived lumbering practices at the turn of the century by taking refuge beneath large emergent rocks and narrow ravines with Rhododendron. This study examined environmental factors associated with emergent rocks that might regulate the distribution of P. …

Cyperus Fuscus (Cyperaceae), New To Missouri And Nevada, With Comments On Its Occurrence In North America, Paul M. Mckenzie, Brad Jacobs, Charles T. Bryson, Gordon C. Tucker, Richard Carter

Faculty Research & Creative Activity

No abstract provided.