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Nucleic Acid Binding Characterization Of The Gar Domain Of Nucleolin, Adlah Sukkar

Honors Capstones

No abstract provided.

Short-Eared Owl Post-Fledging Survival And Breeding Season Diet, Thomas A. Rivest

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

My research primarily focused on the survival of short-eared owls (Asio flammeus) from leaving the nest until their emigration from their natal territories during 1993 and 1994. I attached tarsal-mounted radio transmitters to nestlings prior to fledging. Of 25 fledgling short-eared owls monitored, 16 (64%) died prior to dispersal and one (4%) died after dispersing. Mammalian predation (5, 29.4%) was the primary cause of mortality followed by starvation (4, 23.5%), exposure (2, 11.8%), auto collision (1, 5.9%), burial (1, 5.9%), and unknown causes (4, 23.5%). Hatch date was found to be negatively correlated with fledgling survival.

A secondary …

Bahamian Reef Communities: Composition, Recruitment And Change (10 Meters To 250 Meters), William E. Avery

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The composition, recruitment rates and fine-scale spatial dynamics of the sessile benthic community occupying hard substrata off Lee Stocking Island, The Bahamas, was determined through photography and the deployment of artificial substrata from September 1993 to September 1996. The study spanned 10 to 250 meters in depth. The benthic community exhibited a pronounced bathymetric zonation. Filamentous and macroalgae dominated the shallow communities, but were replaced by corals, sponges and endolithic algae with increasing depth. Living cover was high above 100 m, but declined significantly below this depth, being replaced by increasing amounts of bare and sediment-covered rock surfaces. Benthic zonation …

Changes In The Breeding Bird Community Of Subtropical Evergreen Forest In The Lower Rio Grande Valley Of Texas, 1970s-1990s, Timothy Brush, Alberto Cantu

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Breeding-bird censuses conducted in 1994-1996 at the Santa Ana National Wildlife, southern Texas were compared to those done on the same site in 1973-1978. The study site was described as subtropical evergreen forest in the 1970s, but is now thorn-forest with remnant evergreen forest. Canopy cover was incomplete and lower and trees were smaller and more densely packed in 1994-1996 than in 1973-1978. Seven bird species typical of thorn-forest and thorn-scrub, which were not present in 1973-1978, made up 20% of the breeding bird community in 1994-1996. Species tolerant of thorn-forest and thorn-scrub made up 76.0% of the breeding bird …

Temperature Sensitivity Of El Tor 01, Classical 01, And 0139 Vibrio Cholerae, Michelle Angela O'Neill

Dissertations and Theses

Vibrio cholerae has long been recognized as the causative agent of the disease cholera, but what has only become evident in recent years is the role of the environment in cholera. The cholera bacterium is found autochthonously in the marine and estuarian environment, and if there is any hope of controlling the disease the environmental aspects must be understood.

The central questions addressed in this study were how bacterial survival was influenced by temperature and the role that available nutrients play in V. cholerae survival. Survival of three different V. cholerae strains [classical (01); El Tor (01), and El Tor …

Organic Compounds In Cyanea Capillata And Chrysaora Quinquecirrha, Kris Mitchell '98

Honors Projects

Cyanea capillata and Chrysaora quinquecirrha are two species of jellyfish which emit an "iodoform-like" odor. Attempts to identify the compound(s) which produce this odor have been made using chromatography techniques. Thus far, results obtained from column chromatography and GC-MS analysis have been inconclusive. However, these data suggest an hypothesis that may explain the origin of the odor. A low molecular-weight, highly volatile compound, such as ethylene, is a likely candidate for this elusive, odor-causing chemical.

Sustainable Development In The Third World: An Assessment Of The Conservation Actions In South Africa, Brazil, Malaysia, And Madagascar, Carol Rizkalla

Honors Theses

As a library research project, this thesis has gone beyond research in the library, and it could not have been possible without the aid of several people. First I must thank the organizations which responded to my request for information: the Center for Environmental Information, the Rainforest Action Network, the World Wildlife Fund, and Conservation International Specifically, Jill McLaughlin in the Communications Department of CI was· most gracious in sending me management plans for parks in Brazil and Madagascar. Carla Langeveld of the WWF, was very helpful during my visit to their library, and subsequently in my request for the …

The Effects Of Tactile/Kinesthetic Instructional Strategies In The Biology Classroom, Janice Batten Sees

Theses and Dissertations

The purpose of this study was to determine whether the addition of tactile/kinesthetic instruction to the biology classroom could significantly increase learning. Various kinds of tactile/kinesthetic lessons were taught to three experimental groups who also received visual and auditory instruction. These tactile/kinesthetic lessons included task cards, task puzzles, manipulatives, total body movement, and large floor games. The control group only received visual and auditory instruction. The subjects were 84 College Biology students from Buena Regional High School, a school in rural South Jersey. The classes varied in gender and race. The same instructor taught all classes and the length of …

Experimental Manipulation Of The Cost Of Thermal Acclimation In Drosophila Melanogaster, Robert A. Krebs, Martin E. Feder

Biological, Geological, and Environmental Faculty Publications

Acclimation to environmental change can impose costs on organisms. One potential cost is the energy and nutrients consumed by a physiological response, e.g. the resources required for expression of heat-shock proteins (Hsps). We examined the significance of this cost by genetic manipulation. We isolated four isofemale lines from a Drosophila melanogaster population previously transformed with a hsp70-kcZ fusion. Lines were similar in Hsp70 expression but differed in P-galactosidase expression upon heat shock, and replicates of each line were reared on a high quantity and low quantity medium. Multiple heat shock reduced survival in all lines, but did not increase developmental …

The Age Of The Kirkdale Cave Palaeofauna, Donald A. Mcfarlane, Derek C. Ford

WM Keck Science Faculty Papers

The Kirkdale Cave palaeofauna represents the original and classic 'warm', interglacial mammalian cave deposit in Britain. Although long considered to be 'Ipswichian' in age, no previous attempts to obtain radiometric dates have been recorded. Here we report a uranium-series disequilibrium date of 121,000 ± 4000 yr BP on a flowstone capping that overlay the original bone bed. The precision of the date exceeds that obtained at any other British Interglacial cave site, and permits tentative correlation with the high precision ice core records now available.

Energy Absorption During Running By Leg Muscles In A Cockroach, Robert J. Full, Darrell R. Stokes, Anna N. Ahn, Robert K. Josephson

All HMC Faculty Publications and Research

Biologists have traditionally focused on a muscle's ability to generate power. By determining muscle length, strain and activation pattern in the cockroach Blaberus discoidalis, we discovered leg extensor muscles that operate as active dampers that only absorb energy during running. Data from running animals were compared with measurements of force and power production of isolated muscles studied over a range of stimulus conditions and muscle length changes. We studied the trochanter-femoral extensor muscles 137 and 179, homologous leg muscles of the mesothoracic and metathoracic legs, respectively. Because each of these muscles is innervated by a single excitatory motor axon, …

Effects Of Distance To Juniperus Virginiana On The Establishment Of Fraxinus And Acer Seedlings In Old Fields, Scott J. Meiners, David L. Gorchov

Faculty Research & Creative Activity

It has been hypothesized that Juniperus virginiana facilitates tree seedling establishment in secondary succession. To test this hypothesis, we sampled four old fields in southwestern Ohio and monitored experimentally planted seeds and seedlings of Acer saccharum and Fraxinus americana for two years. Seeds and seedlings were placed into herbivore exclosures placed 0.3 and 3.0 m from J virginiana trees in an old field in Ohio. We found a significant positive spatial association between juniperus virginiana and tree seedling densities in all four old fields. Soil temperature, soil moisture, evaporative demand and light level in the 0.3 m treatment were significantly …

Effects Of Distance To Juniperus Virginiana On The Establishment Of Fraxinus And Acer Seedlings In Old Fields, Scott Meiners, David Gorchov

Faculty Research & Creative Activity

It has been hypothesized that Juniperus virginiana facilitates tree seedling establishment in secondary succession. To test this hypothesis, we sampled four old fields in southwestern Ohio and monitored experimentally planted seeds and seedlings of Acer saccharum and Fraxinus americana for two years. Seeds and seedlings were placed into herbivore exclosures placed 0.3 and 3.0 m from J virginiana trees in an old field in Ohio. We found a significant positive spatial association between juniperus virginiana and tree seedling densities in all four old fields. Soil temperature, soil moisture, evaporative demand and light level in the 0.3 m treatment were significantly …

Staphylococcus Succi Nus Sp. Nov., Isolated From Dominican Amber, L. H. Lambert, T. Cox, K. Mitchell, R. A. Rosselló-Mora, C. Del Cueto, D. E. Dodge, P. Orkand, R. J. Cano

Biological Sciences

Two bacterial isolates, designated AMG-D1^Tand AMG-D2, were recovered from 25-35-million-year-old Dominican amber. AMG-D1^Tand AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically from other staphylococci. Fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. Moreover, AMG-D1^Tand AMG-D2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the G+C-rich coryneform bacteria, as opposed to L-lysine characteristic of staphylococci. AMG-D1^Tand AMG-D2 have a G+C ratio of 35 mol%. Phylogenetic analysis with the 16S rRNA gene indicated that AMG-D1^Tand AMG-D2 were most closely …

Summary Of Submissions To Fisheries Management Paper No 108 - Issues Affecting Western Australia's Inshore Crab Fishery, Cathy Campbell

Fisheries Management Papers

The summary of submissions is a record of comments offered in respect to the contet of Fisheries Management Paper No. 108 : Issues affecting Western Australia's inshore crab fishery (the Issues Paper) and submitted by groups or individuals who have an interest in inshore crab fishing activity in this State. Concerns of the respective parties included a percieved decline in recreational crab catches at specific locations; instances of conflict between commerical and recreational crabs fishers in Geographe Bay; concern for the types of gear used to take crabs; a substantial rise in the catch of crabs in the Cockburn Sound …

Breeding Birds At The Idaho National Engineering And Environmental Laboratory, 1985 - 1991, James R. Belthoff, Leon R. Powers, Timothy D. Reynolds

Biology Faculty Publications and Presentations

During the summers of 1985-1991, bird censuses were conducted along 13 permanent routes located at the 2315-km² Idaho National Engineering and Environmental laboratory (INEEL, formerly INEL) in southeastern Idaho. The objectives of the surveys were to (1) compare avifauna in and near facility complex sites with remote, relatively undisturbed habitats, (2) identify trends in populations of sagebrush-obligate species and other common shrug-steppe species, and (3) determine the presence, abundance, and population status of species of special concern. Five routes were official U.S. Geological Survey, Biological Resources Division 40.0-km Breeding Bird Survey (BBS) routes (formerly administered by the U.S. Fish …

The Carg Boxes In The Promoter Of The Arabidopsis Floral Organ Identity Gene Apetala3 Mediate Diverse Regulatory Effects, Joline J. Tilly, David W. Allen, Thomas Jack

Dartmouth Scholarship

APETALA3 is a MADS box gene required for normal development of the petals and stamens in the Arabidopsis flower. Studies in yeast, mammals and plants demonstrate that MADS domain transcription factors bind with high affinity to a consensus sequence called the CArG box. The APETALA3 promoter contains three close matches to the consensus CArG box sequence. To gain insights into the APETALA3 regulatory circuitry, we have analyzed the APETALA3 promoter using AP3::uidA(GUS) fusions. 496 base pairs of APETALA3 promoter sequence 5′ to the transcriptional start directs GUS activity in the same temporal and spatial expression pattern as the APETALA3 RNA …

Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis

Biological Sciences: Faculty Publications

A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent …

Characterization Of Swinepox Virus Secretory Polypeptides, Takeshi Shimamura

Masters Theses

Swinepox virus (SPV) is the only known member of the genus Suispoxvirus. SPV causes a mild disease in swine characterized by slight fever, and weight loss, associated with lesions restricted to the skin. There have been few studies on SPV. Other poxviruses, such as vaccinia virus, have been well characterized and the biological activities of their secretory polypeptides have been analyzed. This study describes a major SPV secretory polypeptide with an apparent molecular mass of 35kDa. Poxvirus encoded polypeptides secreted during infection are known to exhibit immunomodulatory activities. The 35kDa SPV secretory polypeptide was tested.for its ability to inhibit …

Fission Yeast Mitotic Regulator Dsk1 Is An Sr Protein-Specific Kinase, Zhaohua Irene Tang, Mitsuhiro Yanagida, Ren-Jang Lin

WM Keck Science Faculty Papers

Intricate interplay may exist between pre-mRNA splicing and the cell division cycle, and fission yeast Dsk1 appears to play a role in such a connection. Previous genetic analyses have implicated Dsk1 in the regulation of chromosome segregation at the metaphase/anaphase transition. Yet, its protein sequence suggests that Dsk1 may function as a kinase specific for SR proteins, a family of pre-mRNA splicing factors containing arginine-serine repeats. Using an in vitro system with purified components, we showed that Dsk1 phosphorylated human and yeast SR proteins with high specificity. The Dsk1-phosphorylated SF2/ASF protein was recognized strongly by a monoclonal antibody (mAb104) known …

Coreceptor Utilization By Human Immunodeficiency Virus Type 1 Is Not A Primary Determinant Of Neutralization Sensitivity, Rachel A. Lacasse, Kathryn E. Follis, Tarsem Moudgil, Meg Trahey, James M. Binley, Vicente Planelles, Susan Zolla-Pazner, Jack H. Nunberg

Biological Sciences Faculty Publications

We have examined the relationship between coreceptor utilization and sensitivity to neutralization in a primary isolate of human immunodeficiency virus type I and its T-cell line-adapted (TCLA) derivative. We determined that adaptation of the primary-isolate (PI) virus 168P results in the loss of the unique capacity of PI viruses to utilize the CCR5 coreceptor and in the acquisition by the TCLA 168C virus of sensitivity to neutralization by V3-directed monoclonal antibodies (MAbs). In experiments wherein infection by 168P is directed via either the CCR5 or the CXCR4 pathway, we demonstrate that the virus, as well as pseudotyped virions bearing a …

Differential Expression Of The Arabidopsis Nia1 And Nia2 Genes: Cytokinin-Induced Nitrate Reductase Activity Is Correlated With Increased Nia1 Transcription And Mrna Levels, Xiaodan Yu, Sujatha Sukumaran, László Márton

Faculty Publications

Nitrate reductase (NR) activity increased up to 14-fold in response to treatment of Arabidopsis thaliana seedlings with the cytokinin benzyladenine. NR induction was observed in seedlings germinated directly on cytokinin-containing medium, seedlings transferred to cytokinin medium, and seedlings grown in soil in which cytokinin was applied directly to the leaves. About the same level of induction was seen in both wild-type and Nia2-deletion mutants, indicating that increased NR activity is related to the expression of the minor NR gene, Nia1. The steady-state Nia1 mRNA level was increased severalfold in both wild-type and mutant seedlings after benzyladenine treatment. Transcript …

A Cladistic Analysis Of NdhF Sequences From Representative Species Of Saintpaulia And Streptocarpus Subgenera And Streptocarpella (Gesneriaceae), James F. Smith, M. E. Kresge, M. Möller, Q. C. B. Cronk

Biology Faculty Publications and Presentations

Two African genera of the Gesneriaceae, Saintpaulia and Streptocarpus, are similar in many respects. Both genera have blue to purple flowers, pollen of similar shape and exine sculpting, embryos with one-celled uninucleate chalazal haustoria, shared vegetative structures among some species, and are among the few genera in the Gesneriaceae which include species with chromosome count of n=15. Similarity of these features has indicated that the two genera are likely to be closely related. This study examines the sequences of the chloroplast gene ndhF among several representative Saintpaulia and representatives of Streptocarpus subgenera Streptocarpella and Streptocarpus. The …

Experimental Evolution Of Complexity: In Vitro Emergence Of Intermolecular Ribozyme Interactions, Martin M. Hanczyc, Robert L. Dorit

Biological Sciences: Faculty Publications

In the course of evolving variants of the Tetrahymena thermophila Group I ribozyme for improved DNA cleavage in vitro, we witnessed the unexpected emergence of a derived molecular species, capable of acting as a partner for the ribozyme, but no longer autocatalytic. This new RNA species exhibits a deletion in the catalytic core and participates in a productive intermolecular interaction with an active ribozyme, thus insuring its survival in the population. These novel RNA molecules have evolved a precise catalytic interaction with the Group I ribozyme and depend for their survival on the continued presence of active catalysts. This interaction …

Ventilation Behavior Of The Mantis Shrimp, Hemisquilla Ensigera Californiensis In Hypoxic Burrows, Tamara L. Richter

Loma Linda University Electronic Theses, Dissertations & Projects

The burrow-dwelling mantis shrimp, Hemisquilla ensigera californiensis, is an environmentally challenged marine species due to the fact it inhabits burrows located beneath the surface of the sediment where oxygen pressure is low and easily depleted. This experiment revealed that burrow oxygen pressure (pO₂) is frequently hypoxic and can become anoxic. Average pO₂ within the burrow is between 40-50 mm Hg and may drop below 10 mm Hg even if the burrow isn't capped. When capped, p0₂ rapidly drops to 0 mm Hg within two hours. Low pO₂ does not result in decreased animal activity, …

Pollinator Selection, Quantitative Genetics, And Predicted Evolutionary Responses Of Floral Traits In Penstemon Centranthifolius (Scrophulariaceae), Randall J. Mitchell, Ruth G. Shaw, Nickolas M. Waser

Biology Faculty Research

Much of the remarkable diversity of angiosperm flowers is thought to have evolved in response to selection by animal pollinators. Selection during pollination can readily be documented, but the extent to which it leads to evolutionary change depends on the genetic basis of floral traits. Here we combine estimates of genetic variance and covariance for several traits of flowers and inflorescences in a wild plant species, with estimates of natural selection by hummingbird pollinators. Analysis of phenotypic selection through two components of pollination success indicates direct selection of plants toward larger size and toward bearing shorter flowers that produce more …

Myosin Heavy Chain Isoforms And Dynamic Contractile Properties: Skeletal Versus Smooth Muscle, Thomas J. Eddinger

Biological Sciences Faculty Research and Publications

Myosin, one of the primary contractile muscle proteins, displays molecular, enzymatic, structural, functional and regulatory variability. This variability has been shown to account for a significant amount of the functional uniqueness of skeletal and smooth muscle. However, the universal generation of force and/or shortening by these two muscle types belies the ever-increasing number of known distinct differences that bring this about. Thus, the notion that the functional roles of skeletal and smooth muscle, their development and regulation, all appear to be uniquely applicable for their physiological purpose no longer appears heretical. This manuscript presents a cursory overview of the numerous …

Grandmothering, Menopause, And The Evolution Of Human Life Histories, K. Hawkes, J.F. O'Connell, N.G. Blurton Jones, H. Alvarez, Eric Charnov

Biology Faculty & Staff Publications

Long postmenopausal lifespans distinguish humans from all other primates. This pattern may have evolved with mother—child food sharing, a practice that allowed aging females to enhance their daughters fertility, thereby increasing selection against senescence. Combined with Charnov's dimensionless assembly rules for mammalian life histories, this hypothesis also accounts for our late maturity, small size at weaning, and high fertility. It has implications for past human habitat choice and social organization and for ideas about the importance of extended learning and paternal provisioning in human evolution.

The Consequences Of Changing The Top Predator In A Food Web: A Comparative Experimental Approach, Mark A. Mcpeek

Dartmouth Scholarship

Changing the top predator in a food web often results in dramatic changes in species composition at lower trophic levels; many species are extirpated and replaced by new species in the presence of the new top predator. These shifts in species composition also often result in substantial alterations in the strengths of species interactions. However, some species appear to be little affected by these changes that cause species turnover at other positions in the food web. An example of such a difference in species responses is apparent in the distributions of coenagrionid damselflies (Odonata: Zygoptera) among permanent water bodies with …

The Influence Of Habitat Variation On Demography Of Blue Jays (Cyanociita Cristata) In South-Central Florida, Keith Allen Tarvin

USF Tampa Graduate Theses and Dissertations

Vertebrate populations rarely are homogeneous. Instead they exist as arrays of demographically variable subpopulations that are linked through dispersal. Variation in habitat quality is a major cause of demographic variability among subpopulations, but few studies have examined habitat-specific demography empirically. Here I present a detailed field study o f habitat-specific demography in Blue Jays (Cyanocitta cristata) that occupied a habitat mosaic landscape in south-central Florida. By measuring habitat-specific demographic parameters, including density, reproductive output, survival, and dispersal, I tested for the existence of population sources and sinks associated with different habitats, and the applicability ofa series ofhabitat selection models …