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Preliminary Notes On Tachinidae Reared From Lepidoptera In The Ecuadorian Andes, John O. Stireman Iii

Biological Sciences Faculty Publications

Current estimates of the species richness of Tachinidae among geographical provinces suggest that the Neotropical Region harbors the largest number of species and represents a geographic epicenter of tachinid diversification (O’Hara 2006). The Neotropics boasts an impressive fauna consisting of 2864 described species belonging to 822 genera at the time of the Neotropical catalog (Guimarães 1971), making it almost twice as species rich as any other geographic realm (O’Hara 2006; Stireman et al. 2006). This diversity is most apparent at middle elevations (1000– 2000m) along the mountain chains of tropical Central and South America, where tachinids are an abundant and …

Review: A Creationist Review And Preliminary Analysis Of The History, Geology, Climate, And Biology Of The Galápagos Islands, Timothy R. Brophy

Faculty Publications and Presentations

No abstract provided.

Free-Living Male Mountain White-Crowned Sparrows Exhibit Territorial Aggression Without Modulating Total Or Free Plasma Testosterone, Sharon E. Lynn, Thomas P. Hahn, Creagh W. Breuner

Biological Sciences Faculty Publications

In some species, expression of territorial aggression is accompanied by a rise in testosterone secretion, but in others aggressive behavior is expressed while testosterone levels remain unchanged. Corticosteroid binding globulin (CBG) binds both corticosterone and testosterone in avian plasma. Thus, increasing corticosterone may result in fluctuations in unbound ("free") testosterone; this could result in greater biological activity of testosterone without an increase in testosterone secretion. We investigated whether such plasma interactions of testosterone, corticosterone, and CBG might result in alterations of free testosterone in male Mountain White-crowned Sparrows (Zonotrichia leucophrys oriantha). We conducted simulated territorial intrusions during incubation and compared …

Is Selectively Harvested Forest An Ecological Trap For Olive-Sided Flycatchers?, Bruce A. Robertson, Richard L. Hutto

Biological Sciences Faculty Publications

Disturbance-dependent species are assumed to benefit from forestry practices that mimic the appearance of postdisturbance landscapes. However, human activities that closely mimic the appearance but not the fundamental quality of natural habitats could attract animals to settle whether or not these habitats are suitable for their survival or reproduction. We examined habitat selection behavior and nest success of Olive-sided Flycatchers (Contopus cooperi) in a naturally occurring burned forest and an anthropogenically created habitat type-selectively harvested forest. Olive-sided Flycatcher density and nestling provisioning rates were greater in the selectively harvested landscape, whereas estimated nest success in selectively harvested forest was roughly …

Conservation Of B Class Gene Expression In The Second Whorl Of A Basal Grass And Outgroups Links The Origin Of Lodicules And Petals, Clinton Whipple, Michael Zanis, Elizabeth Kellogg, Robert Schmidt

Biology Department Faculty Works

Studies of flower development in core eudicot species have established a central role for B class MADS-box genes in specifying petal and stamen identities. Similarly in maize and rice, B class genes are essential for lodicule and stamen specification, suggesting homology of petals and lodicules and conservation of B class gene activity across angiosperms. However, lodicules are grass-specific organs with a morphology distinct from petals, thus their true homology to eudicot and nongrass monocot floral organs has been a topic of debate. To understand the relationship of lodicules to the sterile floral organs of nongrass monocots we have isolated and …

Discriminant Function Analysis In Marine Ecology: Some Oversights And Their Solutions, J. Wilson White, Benjamin I. Ruttenberg

Biological Sciences

Marine ecologists commonly use discriminant function analysis (DFA) to evaluate the similarity of distinct populations and to classify individuals of unknown origin to known populations. However, investigators using DFA must account for (1) the possibility of correct classification due to chance alone, and (2) the influence of prior probabilities of group membership on classification results. A search of the recent otolith chemistry literature showed that these two concerns are sometimes ignored, so we used simulated data sets to explore the potential pitfalls of such oversights. We found that when estimating reclassification success for a training data set, small sample sizes …

Aphid Thermal Tolerance Is Governed By A Point Mutation In Bacterial Symbionts, Helen E. Dunbar, Alex C. C. Wilson, Nicole R. Ferguson, Nancy A. Moran

Biology Articles and Papers

Symbiosis is a ubiquitous phenomenon generating biological complexity, affecting adaptation, and expanding ecological capabilities. However, symbionts, which can be subject to genetic limitations such as clonality and genomic degradation, also impose constraints on hosts. A model of obligate symbiosis is that between aphids and the bacterium Buchnera aphidicola, which supplies essential nutrients. We report a mutation in Buchnera of the aphid Acyrthosiphon pisum that recurs in laboratory lines and occurs in field populations. This single nucleotide deletion affects a homopolymeric run within the heat-shock transcriptional promoter for ibpA, encoding a small heat-shock protein. This Buchnera mutation virtually eliminates the transcriptional …

The Movement Of The Gastrop Littorina Littorea In The Intertidal Zone During The Onset Of Winter, Jon Lefcheck

Undergraduate Research Symposium (UGRS)

The movement of the snail Littorina littoreaon the North Atlantic coast is poorly understood. Most research has concentrated on the vertical distribution of the snail, and suggests that it prefers the low intertidal zone where its food source is most plentiful. In the winter, this distribution is reinforced by a documented seaward migration of snails from the high intertidal zone in response to falling temperatures. From October 14, 2006 to January 22, 2007, I examined the individual movements and recovery of snails in response to the onset of winter. I proposed that falling water and air temperatures drive the majority …

A Pollen Chase Experiment; Examining Varying Levels Of Embryonic Inbreeding Depression, Emily Wilson, Judy Stone

Undergraduate Research Symposium (UGRS)

A pollen chase experiment was performed upon three Costa Rican populations of Witheringia solanacea to examine the breakdown of genetically enforced self incompatibility (SI) and the extent of embryonic inbreeding depression. Self-pollen was applied in the bud, with outcross pollen applied one day later, and outcross pollinations at both intervals as a control. A variety of responses were found among the populations. BOHS readily accepted self pollen and suffered from very low inbreeding depression. Monteverde and Las Cruces both have lower fruit set with self-pollination precedence indicating that bud pollinations can overcome the self-incompatibility response and that embryonic death due …

Limit Cycle Displacement Model Of Circadian Rhythms, Van D. Gooch

Faculty Working Papers

A mathematical model has been examined that attempts to mimic the effects of changes in environmental conditions on circadian rhythms. The basis of the model claims that for a given set of environmental conditions (e.g., light, temperature and chemical concentrations) there exists a limit cycle that has a given position. When an environmental treatment is applied that is different from the control conditions, the position of the new limit cycle changes and the oscillating parameters of the circadian system are now attracted toward this newly positioned limit cycle. If conditions are subsequently returned back to control levels, the control limit …

Selective Cholesterol Dynamics Between Lipoproteins And Caveolae/Lipid Rafts, Stephen M. Storey, Adalberto M. Gallegos, Barbara P. Atshaves, Avery L. Mcintosh, Gregory G. Martin, Rebecca D. Parr, Kerstin K. Landrock, Ann B. Kier, Judith M. Ball, Friedhelm Schroeder

Faculty Publications

Although low-density lipoprotein (LDL) receptor-mediated cholesterol uptake through clathrin-coated pits is now well understood, the molecular details and organizing principles for selective cholesterol uptake/efflux (reverse cholesterol transport, RCT) from peripheral cells remain to be resolved. It is not yet completely clear whether RCT between serum lipoproteins and the plasma membrane occurs primarily through lipid rafts/caveolae or from non-raft domains. To begin to address these issues, lipid raft/caveolae-, caveolae-, and non-raft-enriched fractions were resolved from purified plasma membranes isolated from L-cell fibroblasts and MDCK cells by detergent-free affinity chromatography and compared with detergent-resistant membranes isolated from the same cells. Fluorescent sterol …

Seasonal And Diel Patterns Of Manatee Habitat Use, Monica Ann Ross

Electronic Theses and Dissertations

State and Federal agencies have created sanctuaries and speed zones to help reduce manatee mortality while incorporating the recreational and commercial resource needs of these same habitats for humans. Specific habitat resources are considered necessary to increase manatee survivorship. We have only recently begun to address how manatees use some of these resources based on physiological or reproductive strategies. In this study, I quantified patterns of habitat use during seasonal and diel periods for different sex and reproductive manatee classes using data from a radio-telemetry study conducted by the Florida Fish and Wildlife Conservation Commission during 1991-1996. I used five …

Role Of Cd36 In Platelet Function, Arunima Ghosh

ETD Archive

CD36 is a scavenger receptor expressed on a wide variety of cells including platelets. It recognizes multiple ligands, yet its function on platelets is incompletely characterized. Endothelial cell-derived microparticles (EMP) have been identified in diseases where platelet activation plays a pivotal role. Because EMP express phosphatidylserine (PS) on their surfaces, a CD36 ligand, we hypothesized that MP may bind to platelets via a PS-CD36 interaction. Human platelets were shown to bind EMP by flow cytometry and fluorescence microscopy. Binding was significantly inhibited by anti-CD36 antibody or using platelets from CD36 null donors. We observed a significant increase in the rate …

Lack Of Melanized Keratin And Barbs That Fall Off: How The Racketed Tail Of The Turquoise-Browed Motmot Eumomota Superciliosa Is Formed, Troy G. Murphy

Biology Faculty Research

The racket-tipped tail of the motmots is uniquely shaped and its formation has attracted much attention. Barbs that grow along the wire of the motmot’s two central tail feathers are weakly attached and shed soon after development. The cause of the weak attachment of these barbs is unclear. I induced feather growth by plucking the central tail feathers from seven turquoise-browed motmots Eumomota superciliosa and then collected the regrown feathers before the barbs along the wire had fully shed. I compared the barb-rachis junction (petiole of the ramus) along the distal flag (the racket-tip of the tail) where barbs are …

Analysis Of A Model Of Nutrient Driven Self-Cycling Fermentation Allowing Unimodal Response Functions, Guihong Fan

Faculty Bibliography

A system of impulsive ordinary differential equations is used to model the growth of microorganisms in a self-cycling fermentor. The microorganisms are being used to remove a non-reproducing contaminant that is limiting to growth at both high and low concentrations. Hence it is the concentration of the contaminant that triggers the emptying and refilling process. This model predicts that either the process fails or the process cycles indefinitely with one impulse per cycle. Success or failure can depend on the choice of microorganisms, the initial concentration of the microorganisms and contaminant, as well as the choice for the emptying/refilling fraction. …

Chronic Acetaminophen Treatment Influences Indices Of Reactive Oxygen Species Accumulation In The Aging Fisher 344 X Brown Norway Rat Aorta, Sarath Meduru

Theses, Dissertations and Capstones

We have previously reported that aging in the Fisher 344 X Brown Norway (FBN) rat aorta is characterized by increased levels of ROS and alterations in cell signaling. Acetaminophen was found to scavenge free radicals in recent ischemia-reperfusion studies. Here we examined if chronic treatment with a therapeutic dose of acetaminophen attenuates age-associated increase in aortic ROS accumulation and signaling. FBN rats (27 month old; n=8) were subjected to 6 months of treatment with a therapeutic dose of acetaminophen (30mg/kg/day), with age-matched untreated FBN rats as controls. Protein oxidation levels were altered in control and treated aortae compared to aortae …

An Ecologically-Significant Range Extension For Hahn's Short-Tailed Fruit Bat (Carolllia Subrufa) In Southwestern Costa Rica, Donald A. Mcfarlane, Keith Christenson

WM Keck Science Faculty Papers

A range extension for Hahn's short-tailed fruit bat, Carollia subrufa, is reported which extends the distribution of the species from the Pacific Dry Forest into the Pacific Moist Forest ecoregion. This raises the possibility that the species may be more widely distributed in Costa Rica than currently supposed, adding almost 30,000 km² of potential range.

Spatial And Temporal Expression Of Vegetation And Atmospheric Variability From Stable Carbon And Nitrogen Isotope Analysis Of Bat Guano In The Southern United States, Christopher M. Wurster, Donald A. Mcfarlane, Michael I. Bird

WM Keck Science Faculty Papers

Stable isotopes of faeces contain information related to the animals feeding ecology. The use of stable isotope values from subfossil faeces as a palaeoenvironmental indicator depends on how faithfully the animal records their local environment. Here we present insectivorous bat guano δ¹³C and δ¹⁵N values from a precipitation gradient across the southern United States and northern Mexico to compare with local vegetation and climate. We find δ¹³C values to be an excellent predictor of expected C₄/CAM vegetation, indicating that the bats are non-selective in their diet. Moreover, we find bat guano δ …

Furman Lake Bathymetry Map, Wade Worthen

Furman Lake Restoration Book Gallery

Aerial view of the Furman Lake showing the depth of the water.

The Abundance Of Different Dragonfly Species At Furman Lake - 2007, Wade Worthen

Furman Lake Restoration Book Gallery

A line graph showing the abundance of fifteen different species of dragonflies spotted over a five month period at Furman Lake in 2007.

Quantifying Hydrologic Fluxes To A Small Impounded Piedmont Lake, Greenville, South Carolina, Cameron Moore

Furman Lake Restoration Book Gallery

A presentation with text, charts, and images showing research gathered over a three month period in 2007 measuring the water flowing into and out of the Furman Lake. The research is pertinent to the Furman Lake Restoration Project as it will help assess the condition of the lake and plan for the future.

Delimitation Of Major Lineages Within Cuscuta Subgenus Grammica (Convolvulaceae) Using Plastid And Nuclear Dna Sequences, Saša Stefanović, Maria Kuzmina, Mihai Costea

Biology Faculty Publications

Subgenus Grammica, the largest and most diverse group in the parasitic genus Cuscuta, includes ~130 species distributed primarily throughout the New World, with Mexico as its center of diversity. To circumscribe the subgenus ans assess the relationships among its major lineages, we conducted the first phylogenetic study of Grammica using plastid trnL F and nrITS sequences from a wide taxonomic sampling covering its morphological, physiological, and geographical diversiity. With the exception of of one species belonging elsewhere, the subgenus was found to be monophyletic. The results further indicate the presence of 15 well supported major clades within Grammica …

Physical Contests For Females In The Japanese Beetle, Popillia Japonica, Paul Switzer, Kipp Kruse

Faculty Research & Creative Activity

We conducted field observations of physical competition for mates, in which a single male attempts to usurp a female from another male, in male Japanese beetles, Popillia japonica Newman (Coleoptera; Scarabaeidae). Physical contests for mates were relatively rare, but when they occurred the challenger male was able to successfully takeover females by dislodging the previously paired resident male in only 18% of contests, suggesting that a substantial prior residency advantage exists in this species. Challenger males that were successful in takeover attempts were significantly larger than the resident male. In contrast, no size pattern was found between intruding males and …

The Venom Gland Transcriptome Of The Desert Massasauga Rattlesnake (Sistrurus Catenatus Edwardsii): Towards An Understanding Of Venom Composition Among Advanced Snakes (Superfamily Colubroidea), Susanta Pahari, Stephen P. Mackessy, R. Manjunatha Kini

Biological Sciences Faculty Publications

BACKGROUND: Snake venoms are complex mixtures of pharmacologically active proteins and peptides which belong to a small number of superfamilies. Global cataloguing of the venom transcriptome facilitates the identification of new families of toxins as well as helps in understanding the evolution of venom proteomes.

RESULTS: We have constructed a cDNA library of the venom gland of a threatened rattlesnake (a pitviper), Sistrurus catenatus edwardsii (Desert Massasauga), and sequenced 576 ESTs. Our results demonstrate a high abundance of serine proteinase and metalloproteinase transcripts, indicating that the disruption of hemostasis is a principle mechanism of action of the venom. In addition …

Production Of Methanethiol And Volatile Sulfur Compounds By The Archaeon ‘‘Ferroplasma Acidarmanus’’, David J. Baumler, Kai F. Hung, Kwang Cheol Jeong, Charles W. Kaspar

Faculty Research & Creative Activity

Acidophiles are typically isolated from sulfaterich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown ‘‘Ferroplasma acidarmanus’’ showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/ MS determined that the volatile sulfur compound emitted by active ‘‘F. acidarmanus’’ cultures is methanethiol. In ‘‘F. acidarmanus’’ cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and …

Growth Parameters For Syringomycin Production In Pseudomonas Syringae Strain M1, Nathaly M. Carranza

Biology Posters

The cyclic lipodepsinonapeptide syringomycin E (SRE) produced by Pseudomonas syringae is being developed as a natural product agrofungicide. The purpose of this research is to identify the most effective P. syringae growth parameters for SRE production. P. syringae strain M1 was grown in 2L capacity fermenters and in capped test tubes both with agitation. A variety of compounds, including amino acids that are components of SRE, were added to the growth medium to study their effects on SRE production. L-histidine was found to be the best amino acid for promoting SRE production. Strain M1 extracts had strong antifungal activities. SRE …

Motion-Sensitive 3-D Optical Coherence Microscope Operating At 1300 Nm For The Visualization Of Early Frog Development, Barbara M. Hoeling, Stephanie S. Feldman, Daniel T. Strenge, Aaron Bernard, Emily R. Hogan, Daniel C. Petersen, Scott E. Fraser, Yun Kee, J. Michael Tyszka, Richard C. Haskell

All HMC Faculty Publications and Research

We present 3-dimensional volume-rendered in vivo images of developing embryos of the African clawed frog Xenopus laevis taken with our new en-face-scanning, focus-tracking OCM system at 1300 nm wavelength. Compared to our older instrument which operates at 850 nm, we measure a decrease in the attenuation coefficient by 33%, leading to a substantial improvement in depth penetration. Both instruments have motion-sensitivity capability. By evaluating the fast Fourier transform of the fringe signal, we can produce simultaneously images displaying the fringe amplitude of the backscattered light and images showing the random Brownian motion of the scatterers. We present time-lapse movies of …

Review Of Analysis Of Phylogenetics And Evolution With R By Emmanuel Paradis, Daniel M. Stoebel

All HMC Faculty Publications and Research

This is a book review.

Nitric Oxide And Cgmp Signaling In Calcium-Dependent Development Of Cell Polarity In Ceratopteris Richardii, Mari L. Salmi, Kacey E. Morris, Stanley J. Roux, D. Marshall Porterfield

Biological Sciences Faculty Research & Creative Works

Single-celled spores of the fern Ceratopteris richardii undergo gravity-directed cell polarity development that is driven by polar calcium currents. Here we present results that establish a role for nitric oxide (NO)/cGMP signaling in transducing the stimulus of gravity to directed polarization of the spores. Application of specific NO donors and scavengers inhibited the calcium-dependent gravity response in a dose-dependent manner. the effects of NO donor exposure were antagonized by application of NO scavenger compounds. Similarly, the guanylate cyclase inhibitors 6-anilino-5,8- quinolinedione and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin, and the phosphodiesterase inhibitor Viagra, which modulate NO-dependent cGMP levels in the cells, disrupted gravity-directed cell polarity …

Genetic Modification Of Host Acceptance By A Seed Beetle: Quasi-Natural Vs. Artificial Selection, Meagan Bridges, Jake Jones, Amberleigh Muller, Frank J. Messina

Biology Posters

Many plant-feeding insects use a fairly well-defined set of hosts, but are occasionally observed on an atypical host. The seed beetle Callosobruchus maculatus infests several grain legumes, especially Vigna spp. It has rarely been reported to attack lentil, a distant relative of its typical hosts (Credland, 1987). In three previous mass-selection experiments, we showed that larval survival in lentil increased rapidly after a bottleneck, from 70% in only 5-7 generations. Host shifts by insects may also require modification of egg-laying behavior. Initial assays confirmed that most C. maculatus females fail to recognize lentil as a potential host. We used both …