Digital Commons Network^™

A Novel Lux Operon In The Cryptically Bioluminescent Fish Pathogen Vibrio Salmonicida Is Associated With Virulence, Eric J. Nelson, Hege S. Tunsjø, Pat M. Fidopiastis, Henning Sørum, Edward G. Ruby

Biological Sciences

The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated with virulence. Cloning and sequencing of the V. salmonicida lux operon revealed that homologs of all of the genes required for luminescence are present: luxAB (luciferase) and luxCDE (aliphatic-aldehyde synthesis). The arrangement and sequence of these structural lux genes are conserved compared to those in related species of …

Genome Size Evolution In Relation To Leaf Strategy And Metabolic Rates Revisited, Jeremy M. Beaulieu, Ilia J. Leitch, Charles A. Knight

Biological Sciences

• Background and AimsIt has been proposed that having too much DNA may carry physiological consequences for plants. The strong correlation between DNA content, cell size and cell division rate could lead to predictable morphological variation in plants, including a negative relationship with leaf mass per unit area (LMA). In addition, the possible increased demand for resources in species with high DNA content may have downstream effects on maximal metabolic efficiency, including decreased metabolic rates.

• MethodsTests were made for genome size-dependent variation in LMA and metabolic rates (mass-based photosynthetic rate and dark respiration rate) using our own …

Kin Selection Does Not Explain Male Aggregation At Leks Of 4 Manakin Species, Bette Loiselle, Thomas Ryder, Renata Durães, Wendy Tori, John Blake, Patricia Parker

Biology Department Faculty Works

In lek-mating systems, males aggregate at display arenas and females visit solely for the purpose of mating. This breeding system is characterized by high variance in male mating success with one male often receiving most copulations. High reproductive skew among males has led to question why males join leks when their chances of reproductive success are so low. Kin selection has been invoked as a mechanism to explain the evolution of lekking behavior, whereby nonreproducing but genetically related males gain indirect inclusive-fitness benefits. Evidence for kin selection among lek-mating birds is, however, mixed. Here, we show that kin selection is …

Invasion By Aegilops Triuncialis (Barb Goatgrass) Slows Carbon And Nutrient Cycling In A Serpentine Grassland, Rebecca E. Drenovsky, Katharine M. Batton

Biology

Invasive plant species alter plant community composition and ecosystem function. In the United States, California native grasslands have been displaced almost completely by invasive annual grasses, with serpentine grasslands being one of the few remaining refugia for California grasslands. This study examined how the invasive annual grass, Aegilops triuncialis, has altered decomposition processes in a serpentine annual grassland. Our objectives were to (1) assess howA. triuncialis alters primary productivity and litter tissue chemistry, (2) determine whether A. triuncialis litter is more recalcitrant to decomposition than native litter, and (3) evaluate whether differences in the soil microbial community in A. triuncialis-invaded …

Esa And Environmental Justice, George Middendorf, Charles Nilon

Department of Biology Faculty Publications

No abstract provided.

Correction: African American Mitochondrial Dnas Often Match Mtdnas Found In Multiple African Ethnic Groups, Bert Ely, Jamie Wilson, Fatimah Linda Collier Jackson, Bruce Jackson

Department of Biology Faculty Publications

After the publication of this work [1], we became aware that AFDIL data set used to construct our database of sub-Saharan mtDNA sequences had been mislabelled, and in fact, did not contain Sierra Leone mtDNA sequences. We have obtained the correct Sierra Leone data set from AFDIL, reconstructed the database using the new file, and reanalyzed all of the data. The size of our database was reduced from 3725 to 3717 since the new Sierra Leone data set contained 109 sequences instead of 117 in themislabelled data set. The swapping of data sets resulted in a cascade of minor corrections …

Spatial Variation In Sea Urchins, Fish Predators, And Bioerosion Rates On Coral Reefs Of Belize, Jocelyn Brown-Saracino, Paulette Peckol, H. Allen Curran, Martha L. Robbart

Biological Sciences: Faculty Publications

Although sea urchins are critical for controlling macroalgae on heavily fished coral reefs, high densities threaten reefs, as urchins are also prodigous bioeroders. This study examined urchin population characteristics, bioerosion rates, their fish predators (Labridae), and potential competitors (Scaridae) on unprotected reefs and a reef within a marine protected area (MPA) in the lagoonal regions off Belize. Urchin density (<1 >m-2) and bioerosion rates (∼0.2 kg CaCO3m-2year-1) were lowest and members of the Labridae were the highest (∼20 fish 200 m-3) within the MPA, while several unprotected reefs had higher (∼18-40 m-2) urchin densities, lower Labridae abundances (1-3 fish 200 …

The Evolution Of Zinjanthropus Boisei, Paul J. Constantino, Bernard A. Wood

Biological Sciences Faculty Research

Many people assume that OH 5, the type specimen of Paranthropus boisei, collected in 1959, was the first evidence of that taxon to be found, but OH 3, recovered in 1955, predated the discovery of OH 5 by four years. Thus, Paranthropus boisei recently celebrated the equivalent of its fiftieth birthday. This review marks that milestone by examining the way our understanding of this taxon has changed during its fifty, or so, year history.

The Effects Of Seeding Time On Native Plant Establishment In A Prairie Reconstruction, Dave W. Williams, Daryl D. Smith

Faculty Work

The Iowa DOT is seeking ways to improve practices associated with revegetation projects to increase establishment of native grasses and forbs. One of these practices is to seed native grasses and forbs between Apr 01 - June 30 and between Aug. 01 - Aug. 31 (Iowa DOT 2001). We investigated seeding at different times during the growing season to determine the most appropriate seeding time for optimal establishment of prairie grasses and wildflowers.

Notch Signaling Controls The Differentiation Of Transporting Epithelia And Multiciliated Cells In The Zebrafish Pronephros, Yan Liu, Narendra Pathak, Albrecht Kramer-Zucker, Iain A. Drummond

Neuroscience: Faculty Publications

Epithelial tubules consist of multiple cell types that are specialized for specific aspects of organ function. In the zebrafish pronephros, multiciliated cells (MCCs) are specialized for fluid propulsion, whereas transporting epithelial cells recover filtered-blood solutes. These cell types are distributed in a 'salt-and-pepper' fashion in the pronephros, suggesting that a lateral inhibition mechanism may play a role in their differentiation. We find that the Notch ligand Jagged 2 is expressed in MCCs and that notch3 is expressed in pronephric epithelial cells. Morpholino knockdown of either jagged 2 or notch3, or mutation in mind bomb (in which Notch signaling is impaired), …

Mid-Spring Burning Reduces Spotted Knapweed And Increases Native Grasses During A Michigan Experimental Grassland Establishment, Neil W. Macdonald, Brian T. Scull, Scott R. Abella

Peer Reviewed Publications

The definitive version is available at www.blackwell-synergy.com Infestations of the exotic perennial Spotted knapweed (Centaurea maculosa Lam.) hinder the restoration and management of native ecosystems on droughty, infertile sites throughout the Midwestern United States. We studied the effects of annual burning on knapweed persistence on degraded, knapweed-infested gravel-mine spoils in western Michigan. Our experiment included 48, 4-m² plots seeded to native warm-season grasses in 1999 using a factorial arrangement of initial herbicide and fertility treatments. Beginning in 2003, we incorporated fire as an additional factor and burned half of the plots in late April or May for three …

Activation Of Extracellular Signal-Regulated Kinase 5 Reduces Cardiac Apoptosis And Dysfunction Via Inhibition Of A Phosphodiesterase 3a/Inducible Camp Early Repressor Feedback Loop, Chen Yan, Bo Ding, Tetsuro Shishido, Chang Hoon Woo, Seigo Itoh, Kye Im Jeon, Weimin Liu, Haodong Xu, Carolyn Mcclain, Carlos Molina, Burns C. Blaxall, Jun Ichi Abe

Department of Biology Faculty Scholarship and Creative Works

Substantial evidence suggests that the progressive loss of cardiomyocytes caused by apoptosis significantly contributes to the development of heart failure. β-Adrenergic receptor activation and subsequent persistent phosphodiesterase 3A (PDE3A) downregulation and concomitant inducible cAMP early repressor (ICER) upregulation (PDE3A/ICER feedback loop) has been proposed to play a key role in the pathogenesis of cardiomyocyte apoptosis. In contrast, insulin-like growth factor-1 can activate cell survival pathways, providing protection against cell death and restoring muscle function. In this study, we found that insulin-like growth factor-1 activates extracellular signal-regulated kinase 5 (ERK5) and inhibits PDE3A/ICER feedback loop. Insulin-like growth factor-1 normalized isoproterenol-mediated PDE3A …

The Carnegie Protein Trap Library: A Versatile Tool For Drosophila Developmental Studies, Anna Allen

Department of Biology Faculty Publications

Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell resolution. By sequencing the genomic insertion sites, determining splicing patterns downstream of the enhanced green fluorescent protein (EGFP) exon, and analyzing expression patterns in the ovary and salivary gland, we found that 600-900 different genes are trapped in our collection. A core set of 244 lines trapped different identifiable protein isoforms, while insertions likely to act as GFP-enhancer traps were …

The Molecular Basis Of High-Altitude Adaptation In Deer Mice, Jay F. Storz, Stephen J. Sabatino, Federico G. Hoffmann, Eben Gering, Hideaki Moriyama, Nuno Ferrand, Bruno Monteiro, Michael W. Nachman

Biology Faculty Articles

Elucidating genetic mechanisms of adaptation is a goal of central importance in evolutionary biology, yet few empirical studies have succeeded in documenting causal links between molecular variation and organismal fitness in natural populations. Here we report a population genetic analysis of a two-locus α-globin polymorphism that underlies physiological adaptation to high-altitude hypoxia in natural populations of deer mice, Peromyscus maniculatus. This system provides a rare opportunity to examine the molecular underpinnings of fitness-related variation in protein function that can be related to a well-defined selection pressure. We surveyed DNA sequence variation in the duplicated α-globin genes of P. maniculatus …

Hox Targeting In Vertebrate Forelimb Induction: Expression And Comparative Genomics, Lee Fitzhugh Greer Iii

Loma Linda University Electronic Theses, Dissertations & Projects

Hox genes encode master regulators of body plan during embryonic development. In land and avian (bird) vertebrates there are four clusters of Hox genes (A, B, C, D). Patterns of Hox expression (paralogs 5-8), in our work specifically Hoxc5, c6, c8, and Hoxd8 in the forelimb forelimb area have been determined, using the chicken (Gallus gallus) as our model organism. These data help complete the known Hox gene expression patterns for early forelimb development for all four land and avian vertebrates. Potential Hox, Box-associated, and homeodomain core binding sites in genes inducing the forelimb (Tbx5, Salli, Sall4, …

Counterproductive Mix Of Science And Theology: Review Of Astronomy And The Bible - Questions And Answers By Donald B. Deyoung, William D. Stansfield

Biological Sciences

No abstract provided.

Availability Of Dissolved Organic Matter Offsets Metabolic Costs Of A Protracted Larval Period For Bugula Neritina (Bryozoa), Collin H. Johnson, Dean E. Wendt

Biological Sciences

For nearly a century researchers have investigated the uptake and utilization of dissolved organic matter (DOM) by marine invertebrates, but its contribution to their growth, reproduction, and survival remains unclear. Here, the benefit of DOM uptake was assessed for the marine bryozoan Bugula neritina (Linnaeus 1758) through performance comparisons of individuals in the presence and absence of DOM. The experiments were performed using B. neritina collected from floating docks in Beaufort, NC, USA from July to September 2004. Seawater was subjected to ultraviolet irradiation to reduce naturally occurring DOM, and then enriched with either 1 μM of palmitic acid or …

Interspecific And Intraspecific Differences In Foraging Preferences Of Container-Dwelling Mosquitoes, Kesavaraju Banugopan, Donald A. Yee, Steven A. Juliano

Faculty Publications – Biological Sciences

Feeding preferences of larval container-dwelling mosquitoes are not well understood. Primary production is often absent in container systems and external inputs of animal and plant detritus supply the energy base of container food webs by supporting microorganism prey for mosquitoes. We quantified the feeding preferences of Aedes albopictus (Skuse), a non-native invasive mosquito, and Ochlerotahts triseriatus (Say), a native mosquito, when given a choice of animal and plant detritus. We tested for interpopulational (Illinois versus Florida) differences in feeding preferences, and quantified each species' performance on these two detritus types. When given a choice, both species spent significantly more time …

Sedimentology Of Acid Saline Lakes In Southern Western Australia: Newly Described Processes And Products Of An Extreme Environment, Kathleen Counter Benison, Brenda Beitler Bowen, Francisca Oboh-Ikuenobe, Elliot A. Jagniecki, Deidre A. Laclair, Stacy L. Story, Melanie R. Mormile, Boyoung Hong

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Naturally acid saline systems with pH values between 1.7 and 4 are common on the Yilgarn Craton of southern Western Australia. a combination of physical and chemical processes here yield a previously undescribed type of modern sedimentary environment. Flooding, evapoconcentration, desiccation, and eolian transport at the surface, as well as influx of acid saline groundwaters, strongly influence these lakes. Halite, gypsum, kaolinite, and iron oxides precipitate from acid hypersaline lake waters. Shallow acid saline groundwaters affect the sediments of the lakes and associated mudflats, sandflats, channels, and dunes by precipitating early diagenetic halite, gypsum, iron oxides, clays, jarosite, and alunite. …

The Effects Of Seeding Rate Of Prairie Grasses And Forbs On Native Plant Establishment In A Prairie Reconstruction. Iowa Department Of Transportation Final Report., Dave W, Williams, Daryl D. Smith

Faculty Work

The Iowa DOT is seeking ways to improve practices associated with revegetation projects to increase establishment of native grasses and forbs. Iowa DOT has developed various seed mixes for re-vegetation projects (Iowa DOT 2005). Each seed mix has the potential to result in a different plant community. Results from this research project will provide information on the seeding rates for species needed for optimal establishment of grasses and wildflowers in future Iowa DOT prairie plantings as well as determine if current seeding practices are adequate. This information can be used to revise or develop seeding rate recommendations for revegetation projects.

Heritability Of Latent Period Estimated From Wild-Type And Selected Populations Of Puccinia Triticina, Jeffrey Lehman

Biology and Earth Science Faculty Scholarship

Durability of partially resistant wheat cultivars to wheat leaf rust depends on the amount of genetic variation in parasitic fitness within populations of the pathogen Puccinia triticina. To assess the durability of partial resistance, selection experiments were used to explore quantitative variation in parasitic fitness of P. triticina. Fungal populations 881-WT and 882-WT were selected for shortened latent period on partially resistant cvs. CI 13277 and Sw 72469-6 for multiple generations. Fitness components were measured for wild-type and selected fungal populations. Responses to selection and selection differentials were calculated, and broad-sense, realized heritabilities for latent period were estimated for wild-type …

Run-Specific Limits Of Quantitation And Detection (An Alternative To Minimum Peak Height Thresholds), Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Assessing The Implications For Close Relatives In The Event Of Similar But Non-Matching Dna Profiles, Dan E. Krane

Biological Sciences Faculty Publications

No abstract provided.

Community Ecology Of Bats In Southern Lower Michigan, With Emphasis On Roost Selection By Myotis, Lisa Winhold

Master's Theses and Doctoral Dissertations

I studied the roosting niche of three sympatric species of Myotis (little brown bat, M. lucifugus; northern bat, M. septentrionalis; and Indiana bat, M. sodalis) and examined changes in composition of the entire bat community in southern Lower Michigan over long periods. Little brown bats roosted in buildings, whereas northern and Indiana bats used trees. Northern and Indiana bats differed primarily in species of tree used, whether the tree was living or dead, and use of cavities or loose bark. There were no differences among species in composition of landscapes surrounding roosts. I also netted bats during 2004–2006 and compared …

Is Oak Establishment In Old‐Fields And Savanna Openings Context Dependent?, Ian A. Dickie, Stefan A. Schnitzer, P. B. Reich, S. E. Hobbie

Biological Sciences Faculty Research and Publications

1. Multiple factors are known to influence tree seedling establishment, yet the degree to which these factors depend on each other and on spatial context is largely unknown. We examined the influence of herbaceous competition and water and nitrogen limitations on tree seedling establishment as functions of distance from trees (within‐site spatial context) and site history (between‐site spatial context; as old‐fields vs. savanna openings).
2. We grew Quercus ellipsoidalis E.J. Hill (pin oak) and Q. macrocarpa Michx. (bur oak) seedlings for 3 years in abandoned agricultural fields and savannas in central Minnesota, USA, near and distant from adult oak trees, with and …

Ips Pini (Curculionidae: Scolytinae) Is A Vector Of The Fungal Pathogen, Sphaeropsis Sapinea (Coelomycetes), To Austrian Pines, Pinus Nigra (Pinaceae), Justin G.A. Whitehall, Jeffery S. Lehman, Pierluigi Bonello

Biology and Earth Science Faculty Scholarship

Sphaeropsis sapinea (Fr.:Fr.) Dyko and Sutton, is among the most common and widely distributed pathogens of conifers worldwide. S. sapinea is disseminated over short distances by rain splash and moist wind, but significant knowledge gaps regarding long-range dispersal remain. Our objective was to determine whether or not the pine engraver beetle, Ips pini Say, is a vector of the pathogen onto Austrian pines (Pinus nigra Arnold). In 2004 and 2005, individuals of I. pini were collected with pheromone traps at two locations in central Ohio (197 and 1,017 individuals for 2004 and 2005, respectively) and screened for the presence of …

Integrated Fisheries Management Allocation Report Western Rock Lobster Resource, Integrated Fisheries Allocation Advisory Committee

Fisheries Management Papers

This report, prepared by the Integrated Fisheries Allocation Advisory Committee (IFAAC), contains the committee’s advice and recommendations to the Minister for Fisheries on allocations for the western rock lobster resource. The setting of explicit allocations to sectors (Customary, recreational and commercial) is integral to Integrated Fisheries Management (IFM) in Western Australia.

Management Of The Houtman Abrolhos System. A Draft Review 2007 - 2017, Department Of Fisheries (Western Australia) On Advice From The Abrolhos Islands Management Advisory Committee

Fisheries Management Papers

This review contains a brief description of the Abrolhos System and its special values. It also states the goal and principal objectives for management during the next ten years (2006-2016), along with updated strategies to achieve these objectives. The document succinctly outlines the direction for multiple use management of the Houtman Abrolhos Islands as determined by the Abrolhos Islands Management Advisory Committee (AIMAC). The Committee’s role is to advise the Minister for Fisheries on management of the Abrolhos Islands and the Abrolhos Islands Fish Habitat Protection Area.

Urban Physiology: City Ants Possess High Heat Tolerance, Michael W. Sears, Michael J. Angilletta, Robbie S. Wilson, Amanda C. Niehaus, Pedro L. Ribeiro, Carlos A. Navas

Publications

Urbanization has caused regional increases in temperature that exceed those measured on a global scale, leading to urban heat islands as much as 12°C hotter than their surroundings. Optimality models predict ectotherms in urban areas should tolerate heat better and cold worse than ectotherms in rural areas. We tested these predications by measuring heat and cold tolerances of leaf-cutter ants from South America’s largest city (São Paulo, Brazil). Specifically, we compared thermal tolerances of ants from inside and outside of the city. Knock-down resistance and chill-coma recovery were used as indicators of heat and cold tolerances, respectively. Ants from within …