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A Comparative Study On Pleurotus Ostreatus (Jacq.) P.Kumm. Cultivated On Different Agricultural Lignocellulosic Wastes, Abdurrahman Dündar, Abdunnasir Yildiz Jan 2009

A Comparative Study On Pleurotus Ostreatus (Jacq.) P.Kumm. Cultivated On Different Agricultural Lignocellulosic Wastes, Abdurrahman Dündar, Abdunnasir Yildiz

Turkish Journal of Biology

For the cultivation of Pleurotus ostreatus (Jacq.) P.Kumm. wheat stalk (WS), cotton stalk (CS), millet stalk (MS), and soybean stalk (SS) were used as the main material and oven-dried lentil straw (LS) was used as an additive material 100:10, 100:15, 100:20 w:w in weight for 100 g of main material (70% moisture). The shortest mycelium growing time (MGT) was 10.2 days, on the SS, while the longest was 18.8 days, on the CS. The shortest first harvest time (FHT) was 30 days, on SS + LS (90:10; w:w), and the longest was 46.4 days, on CS + LS (100:20; w:w). …

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Genetic Repairing Through Storage Of Gamma Irradiated Seeds In Inbred Maize (Zea Mays L.), Girjesh Kumar, Prashant Kumar Rai Jan 2009

Genetic Repairing Through Storage Of Gamma Irradiated Seeds In Inbred Maize (Zea Mays L.), Girjesh Kumar, Prashant Kumar Rai

Turkish Journal of Biology

Gamma irradiation can induce beneficial as well as deleterious impacts on chromosome behavior in crop plants. The cytogenetic changes occurring due to the storage of inbred seeds after gamma irradiation in the somatic and gametic cells of Zea mays L. were investigated in this study. A wide spectrum of chromosomal anomalies was encountered in somatic and gametic cells of maize that are gamma irradiated, stored (aged), and treated with a combination of both of these treatments. Gamma rays and ageing treatments induced a number of chromosomal anomalies independently, but a combination of the 2 treatments suppressed the frequency of chromosomal …

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Cytogenetic Effects Of Fungicide Applications On Meiosis Of Tomato (Lycopersicon Esculentum Mill.), İlkay Öztürk Çali Jan 2009

Cytogenetic Effects Of Fungicide Applications On Meiosis Of Tomato (Lycopersicon Esculentum Mill.), İlkay Öztürk Çali

Turkish Journal of Biology

In the present study, the cytogenetic effects of the fungicide Agri Fos 400 (400 g/l mono- and di-potassium phosphonate) on meiosis of Lycopersicon esculentum Mill. (Family: Solanaceae) were studied. The fungicide was applied to tomatoes grown in greenhouse conditions at the dosage (400 ml/100 l water) recommended by the manufacturing company and double the recommended dosage (800 ml/100 l water). All dosages of the fungicide Agri Fos 400 caused various abnormalities in meiosis when compared with the control. This situation could lead to a decrease in the productivity of fruits.

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Inhibitory Effects Of Ajowan (Trachyspermum Ammi) Ethanolic Extract On A. Ochraceus Growth And Ochratoxin Production, Pushpa S. Murthy, B. B. Borse, Hafeeza Khanum, P. Srinivas Jan 2009

Inhibitory Effects Of Ajowan (Trachyspermum Ammi) Ethanolic Extract On A. Ochraceus Growth And Ochratoxin Production, Pushpa S. Murthy, B. B. Borse, Hafeeza Khanum, P. Srinivas

Turkish Journal of Biology

Ajowan is an aromatic seed spice that has a medicinal value. In this paper Ajowan Ethanolic Extract (AEE), which was prepared from Ajowan seeds, was assessed for antibacterial and antifungal activity against selected pathogenic bacteria and fungi by agar well diffusion assay. AEE exhibited considerable inhibitory effects against all the organisms tested. Emphasis of the study was on the affect of AEE on the mycelial growth and spore germination of toxigenic fungi A. ochraceus. Cultures were incubated on yeast extract-sucrose (YES) broth, at concentrations of 50, 150, 250 ppm at 25°C. At 250 ppm, AEE completely inhibited germination of spores, …

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Mutagenesis Of Bacillus Thuringiensis Iam 12077 For Increasing Poly (-\Beta-)Hydroxybutyrate (Phb) Production, Pal Adwitiya, Prabhu Ashwini, Arun Kumar Avinash, Rajagopal Badri, Dadhe Kajal, Ponnamma Vomsi, Shivakumar Srividya Jan 2009

Mutagenesis Of Bacillus Thuringiensis Iam 12077 For Increasing Poly (-\Beta-)Hydroxybutyrate (Phb) Production, Pal Adwitiya, Prabhu Ashwini, Arun Kumar Avinash, Rajagopal Badri, Dadhe Kajal, Ponnamma Vomsi, Shivakumar Srividya

Turkish Journal of Biology

A gram-positive bacterium that accumulated PHB was isolated from local garden soil in Bangalore. Based on its morphological and physiological properties, and nucleotide sequence (about 1.5 kb) of its 16S rDNA it was identified as Bacillus thuringiensis IAM 12077. In this study the PHB-producing capacity of putative mutant strains of Bacillus thuringiensis IAM 12077 was evaluated. A Bacillus sp. identified as a Bacillus thuringiensis IAM 12077 strain capable of producing 10%-15% dry cell weight (DCW) PHB when grown in nutrient broth for 48 h was subjected to random mutagenesis [physical (UV) and chemical (acridine orange)]. Among the UV-mutants screened, 19 …

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Productive Replication Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) In Md203 Cell Line, İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, İkbal A. İnce, Zi̇hni̇ Demi̇rbağ Jan 2009

Productive Replication Of Malacosoma Neustria Nucleopolyhedrovirus (Manenpv) In Md203 Cell Line, İsmai̇l Demi̇r, Nurten Gürel, Remzi̇ye Nalçacioğlu, İkbal A. İnce, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

A plaque-purified genotypic variant, derived from a field isolate of the Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey was characterized based on in vitro replication properties in a cell line, Md203 derived from Malacosoma disstria. The life cycle of ManeNPV was studied based on the cytopathic effects (CPEs), polyhedral inclusion body (PIB) formation, budded virus (BV) production, viral DNA replication and polyhedrin protein expression in ManeNPV-infected Md203 cells. Infection of Md203 cells with ManeNPV resulted in cytopathic effects within 24 and 36 h post infection and most cells detachted from the bottom of the culture plate on the fourth day. Typical …

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Pathogenic Bacillus Anthracis In The Progressive Gene Losses And Gains In Adaptive Evolution, Gongxin Yu Jan 2009

Pathogenic Bacillus Anthracis In The Progressive Gene Losses And Gains In Adaptive Evolution, Gongxin Yu

Biology Faculty Publications and Presentations

Background: Sequence mutations represent a driving force of adaptive evolution in bacterial pathogens. It is especially evident in reductive genome evolution where bacteria underwent lifestyles shifting from a free-living to a strictly intracellular or host-depending life. It resulted in loss of function mutations and/or the acquisition of virulence gene clusters. Bacillus anthracis shares a common soil bacterial ancestor with its closely related bacillus species but is the only obligate, causative agent of inhalation anthrax within the genus Bacillus. The anthrax-causing Bacillus anthracis experienced the similar lifestyle changes. We thus hypothesized that the bacterial pathogen would follow a compatible evolution path. …

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Caspase-Cleaved Tar Dna-Binding Protein-43 In Pick’S Disease, Troy T. Rohn, Polina Kokoulina Jan 2009

Caspase-Cleaved Tar Dna-Binding Protein-43 In Pick’S Disease, Troy T. Rohn, Polina Kokoulina

Biology Faculty Publications and Presentations

The hyperphosphorylation and proteolytic modification of the TAR DNA binding protein-43 (TDP-43) is a key finding in a number of neurodegenerative diseases including frontotemporal dementia with ubiquitin-positive inclusions (FTLD-U), amyotrophic lateral sclerosis (ALS), and most recently Alzheimer’s disease (AD). To examine whether proteolytic modifications of TDP-43 is a relevant finding in Pick’s disease, we utilized a novel site-directed caspase-cleavage antibody based upon a known caspase-3 cleavage consensus site within TDP-43 at position 219. Application of this antibody, termed TDP caspase-cleavage product (TDPccp) to postmortem Pick’s disease brain sections revealed the presence of caspase-cleaved TDP-43 in Pick and Hirano bodies predominantly …

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An Exon-Based Comparative Variant Analysis Pipeline To Study The Scale And Role Of Frameshift And Nonsense Mutation In The Human-Chimpanzee Divergence, Gongxin Yu Jan 2009

An Exon-Based Comparative Variant Analysis Pipeline To Study The Scale And Role Of Frameshift And Nonsense Mutation In The Human-Chimpanzee Divergence, Gongxin Yu

Biology Faculty Publications and Presentations

Chimpanzees and humans are closely related but differ in many deadly human diseases and other characteristics in physiology, anatomy, and pathology. In spite of decades of extensive research, crucial questions about the molecular mechanisms behind the differences are yet to be understood. Here I report ExonVar, a novel computational pipeline for Exon-based human-chimpanzee comparative Variant analysis. The objective is to comparatively analyze mutations specifically those that caused the frameshift and nonsense mutations and to assess their scale and potential impacts on human-chimpanzee divergence. Genomewide analysis of human and chimpanzee exons with ExonVar identified a number of species-specific, …

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The Malina Oceanographic Expedition: How Do Changes In Ice Cover, Permafrost And Uv Radiation Impact On Biodiversity And Biogeochemical Fluxes In The Arctic Ocean?, Phillippe Massicotte, Rainer Amon, David Antoine, Philippe Archambault, Sergio Balzano, Simon Bélanger, Ronald Benner, Dominique Boeuf, Annick Bricaud, Flavienne Bruyant, Gwenaëlle Chaillou, Malik Malik, Bruno Charrière, Jing Chen, Hervé Claustre, Pierre Coupel, Nicole Delsaut, David Doxaran, Et. Al. Jan 2009

The Malina Oceanographic Expedition: How Do Changes In Ice Cover, Permafrost And Uv Radiation Impact On Biodiversity And Biogeochemical Fluxes In The Arctic Ocean?, Phillippe Massicotte, Rainer Amon, David Antoine, Philippe Archambault, Sergio Balzano, Simon Bélanger, Ronald Benner, Dominique Boeuf, Annick Bricaud, Flavienne Bruyant, Gwenaëlle Chaillou, Malik Malik, Bruno Charrière, Jing Chen, Hervé Claustre, Pierre Coupel, Nicole Delsaut, David Doxaran, Et. Al.

Faculty Publications

The MALINA oceanographic campaign was conducted during summer 2009 to investigate the carbon stocks and the processes controlling the carbon fluxes in the Mackenzie River estuary and the Beaufort Sea. During the campaign, an extensive suite of physical, chemical and biological variables was measured across seven shelf–basin transects (south-north) to capture the meridional gradient between the estuary and the open ocean. Key variables such as temperature, absolute salinity, radiance, irradiance, nutrient concentrations, chlorophyll-a concentration, bacteria, phytoplankton and zooplankton abundance and taxonomy, and carbon stocks and fluxes were routinely measured onboard the Canadian research icebreaker CCGS Amundsen and from a barge …

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Effective Practices In Undergraduate Stem Education Part 1: Examining The Evidence, Labov B. Jay, Susan R. Singer, Melvin D. George, Heidi A. Schweingruber, Margaret L. Hilton Jan 2009

Effective Practices In Undergraduate Stem Education Part 1: Examining The Evidence, Labov B. Jay, Susan R. Singer, Melvin D. George, Heidi A. Schweingruber, Margaret L. Hilton

Biology Faculty Work

No abstract provided.

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Effect Of Increased Carbon Dioxide Levels On Urushiol Production In Toxicodendron Radicans And Toxicodendron Toxicarium, Meredith Gilbert Jan 2009

Effect Of Increased Carbon Dioxide Levels On Urushiol Production In Toxicodendron Radicans And Toxicodendron Toxicarium, Meredith Gilbert

Theses and Dissertations

It is hypothesized that global warming is at least partially due to increased greenhouse gases, one of which is carbon dioxide. An increase in atmospheric carbon dioxide has been shown to stimulate higher rates of plant productivity, including the production of plant toxins like urushiol. Urushiol, a secondary plant compound produced in members of the Anacardiaceae family, causes dermatitis in sensitive individuals. An increase in urushiol production could increase the reactivity of certain individuals to plants like poison oak and poison ivy. To determine how elevated levels of atmospheric carbon dioxide would effect urushiol production, 16 samples of poison ivy …

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Small Rna-Directed Heterochromatin Formation In The Context Of Development: What Flies Might Learn From Fission Yeast, Kathryn Huisinga, Sarah C.R. Elgin Jan 2009

Small Rna-Directed Heterochromatin Formation In The Context Of Development: What Flies Might Learn From Fission Yeast, Kathryn Huisinga, Sarah C.R. Elgin

Biology Faculty Research

A link between the RNAi system and heterochromatin formation has been established in several model organisms including Schizosaccharomyces pombe and Arabidopsis thaliana. However, the data to support a role for small RNAs and the associated machinery in transcriptional gene silencing in animal systems is more tenuous. Using the S. pombe system as a model, we analyze the role of small RNA pathway components and associated small RNAs in regulating transposable elements and potentially directing heterochromatin formation at these elements in Drosophila melanogaster.

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Vegetation Response To Restoration Treatments In A Former Pine Plantation In North Florida, Ryan C. Meyer Jan 2009

Vegetation Response To Restoration Treatments In A Former Pine Plantation In North Florida, Ryan C. Meyer

UNF Graduate Theses and Dissertations

As the human population in Florida continues to expand, development follows, and tree farms give way to homes and businesses. As parks are established, restoration of these semi-natural plantations may provide critical habitat for species conservation. This study evaluates vegetation response to restoration treatments at two study sites, formerly tree farms, now preserves in NE Florida. Treatments included thinning, clearing, or control (no treatment) within 10m^2 plots. Thinning reduced tree canopy to 20% (2-3 pines/plot) and removed all other vegetation; clearing treatments removed all biomass to bare soil; no herbicides were used. Within these plots richness and abundance was assessed …

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Genetic Variation At Hair Length Candidate Genes In Elephants And The Extinct Woolly Mammoth, Alfred L. Roca, Yasuko Ishida, Nikolaidis Sergios, Sergios-Orestis Kolokotronis, Stephen Fratpietro, Kristin Stewardson, Shannon Hensley, Michele Tisdale, Gennady Boeskorov, Alex D. Greenwood Jan 2009

Genetic Variation At Hair Length Candidate Genes In Elephants And The Extinct Woolly Mammoth, Alfred L. Roca, Yasuko Ishida, Nikolaidis Sergios, Sergios-Orestis Kolokotronis, Stephen Fratpietro, Kristin Stewardson, Shannon Hensley, Michele Tisdale, Gennady Boeskorov, Alex D. Greenwood

Biological Sciences Faculty Publications

Background: Like humans, the living elephants are unusual among mammals in being sparsely covered with hair. Relative to extant elephants, the extinct woolly mammoth, Mammuthus primigenius, had a dense hair cover and extremely long hair, which likely were adaptations to its subarctic habitat. The fibroblast growth factor 5 (FGF5) gene affects hair length in a diverse set of mammalian species. Mutations in FGF5 lead to recessive long hair phenotypes in mice, dogs, and cats; and the gene has been implicated in hair length variation in rabbits. Thus, FGF5 represents a leading candidate gene for the phenotypic differences in hair length …

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A Standardized Rna Isolation Protocol For Yam (Dioscorea Alata L) Cdna Library Construction, Satya S. Narina, Ali I. Mohamed, Robert Asiedu, H. D. Mignouna Jan 2009

A Standardized Rna Isolation Protocol For Yam (Dioscorea Alata L) Cdna Library Construction, Satya S. Narina, Ali I. Mohamed, Robert Asiedu, H. D. Mignouna

Virginia Journal of Science

For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia State University. The RNA isolated using this standardized protocol was high in quality and led to successful good quality cDNA library construction and identification of functional ESTs in yam.

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Distribution And Host Specificity Of Erysiphe Cruciferarum (Powdery Mildew) Attacking Alliaria Petiolata (Garlic Mustard), In Southwestern Ohio, Victoria Lyn Ciola Jan 2009

Distribution And Host Specificity Of Erysiphe Cruciferarum (Powdery Mildew) Attacking Alliaria Petiolata (Garlic Mustard), In Southwestern Ohio, Victoria Lyn Ciola

Browse all Theses and Dissertations

Garlic mustard is an invasive Eurasian biennial that has rapidly spread throughout the United States. In Southwestern Ohio, many garlic mustard populations are infected with the powdery mildew Erysiphe cruciferarum that can reduce the growth and fitness of garlic mustard. E. cruciferarum was assessed using a Decision Tree in regards to its potential use as a biological control agent. I determined the distribution of E. cruciferarum on garlic mustard and I determined the potential risk of E. cruciferarum to native Brassicaceous species and selected crops. I surveyed 19 parks in Southwestern Ohio and recorded the number of diseased garlic mustard …

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Comparative Efficacy Of Bioud To Other Commercially Available Arthropod Repellants Against The Ticks Amblyomma Americanum And Dermacentor Variabilis On Cotton Cloth, Brooke W. Bissinger, Jiwei Zhu, Charles S. Apperson, Daniel E. Sonenshine, D. Wesley Watson, R. Michael Roe Jan 2009

Comparative Efficacy Of Bioud To Other Commercially Available Arthropod Repellants Against The Ticks Amblyomma Americanum And Dermacentor Variabilis On Cotton Cloth, Brooke W. Bissinger, Jiwei Zhu, Charles S. Apperson, Daniel E. Sonenshine, D. Wesley Watson, R. Michael Roe

Biological Sciences Faculty Publications

BioUD is an arthropod repellent that contains the active ingredient 2-undecanone originally derived from wild tomato plants. Repellency of BioUD was compared with five commercially available arthropod repellents against the ticks Amblyomma americanum (L.) and Dermacentor variabilis Say in two-choice bioassays on treated versus untreated cotton cheesecloth. Overall mean percentage repellency against both species was greatest for and did not differ significantly between BioUD (7.75% 2-undecanone) and products containing 98.1% DEET, 19.6% IR3535, and 30% oil of lemon eucalyptus. Products containing 5% and 15% Picaridin and 0.5% permethrin were also repellent compared with untreated controls but to a lesser degree …

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The Effects Of Urbanization On The Structure, Quality, And Diversity Of Cypress Plant Communities In Central Florida, Courtney Knickerbocker Jan 2009

The Effects Of Urbanization On The Structure, Quality, And Diversity Of Cypress Plant Communities In Central Florida, Courtney Knickerbocker

Electronic Theses and Dissertations

The integrity of wetland ecosystems is largely determined by hydrological functionality, degree of connectivity to like ecosystems, and permeability to external influence. Land use changes in upland areas adjacent to wetland ecosystems may influence hydrology and connectivity while introducing novel biotic and abiotic materials. There is an increasing trend toward the use of remote assessment techniques to determine the degree of impact of external influences on adjacent wetlands. Remote assessment and predictive capabilities are provided by indices such as the Landscape Development Intensity Index (LDI) (Brown and Vivas 2005) which may be beneficial in determining site condition, and which have …

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Native Fire Regime As A Reference For Establishing Management Practices, Brean Duncan Jan 2009

Native Fire Regime As A Reference For Establishing Management Practices, Brean Duncan

Electronic Theses and Dissertations

Our understanding of natural fire regimes in human-dominated landscapes is limited. Fire regimes operating in the pyrogenic ecosystems of Florida have been altered by fire suppression and fuel fragmentation. This is especially true of North Merritt Island, Florida, where human impacts have led to an incomplete knowledge of current fire regimes. We know that growing season fires frequently occurred within general return intervals and that many native terrestrial species require fire to remain viable. A 20-year plus period of fire suppression caused structural and compositional changes to vegetation/fuels that led to catastrophic fires and the decline of native species populations …

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Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation, Elizabeth R. Deschene, Michael J. Barresi Jan 2009

Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation, Elizabeth R. Deschene, Michael J. Barresi

Biological Sciences: Faculty Publications

Certain fundamental questions in the field of developmental biology can only be answered when cells are placed in novel environments or when small groups of cells in a larger context are altered. Watching how one cell interacts with and behaves in a unique environment is essential to characterizing cell functions. Determining how the localized misexpression of a specific protein influences surrounding cells provides insightful information on the roles that protein plays in a variety of developmental processes. Our lab uses the zebrafish model system to uniquely combine genetic approaches with classical transplantation techniques to generate genotypic or phenotypic chimeras. We …

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Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok Jan 2009

Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok

Biology Faculty Publications

Recent studies have detected estrogenic compounds in surface waters in North America and Europe. Furthermore, the presence of estrogenic compounds in surface waters has been attributed, in some cases, to the discharge of wastewater treatment plant (WWTP) effluent. The primary objective of the current study was to determine if WWTP effluent contributes estrogens to the surface waters of Nebraska. A second objective of this study was to determine if estrogens were found in concentrations sufficient enough to manifest feminizing effects on fish. These objectives were satisfied by deploying polar organic chemical integrative samplers (POCIS) and caged fathead minnows at eight …

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Timecourse Analysis Of The Effect Of Atrazine On Human Hepg2 Cells, Erin M. Schrunk Jan 2009

Timecourse Analysis Of The Effect Of Atrazine On Human Hepg2 Cells, Erin M. Schrunk

Honors Program Theses

Atrazine is one of the most abundantly used herbicides in the United States. It works by inhibiting photosynthesis in broad leaf and grassy weeds. Consequently, with its frequent use, atrazine can contaminate many drinking water sources due to run-off from treated fields. While many studies have shown detrimental effects occur in various model organisms using very high levels of atrazine, not much is known about what effects occur due to lower levels of atrazine exposure. The current research project analyzed the effects of atrazine on human liver cells (HepG2). Specifically, we focused on the proteins that regulate cell cycle progression …

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The Effects Of Oral Administration Of An Aqueous Extract Of Ficus Bengalensis Stem Bark On Some Hematological And Biochemical Parameters In Rats With Streptozotocin-Induced Diabetes, Mahalingam Gayathri, Krishnan Kannabiran Jan 2009

The Effects Of Oral Administration Of An Aqueous Extract Of Ficus Bengalensis Stem Bark On Some Hematological And Biochemical Parameters In Rats With Streptozotocin-Induced Diabetes, Mahalingam Gayathri, Krishnan Kannabiran

Turkish Journal of Biology

The effects of sub-chronic administration of an aqueous extract of Ficus bengalensis stem bark on hematological and biochemical parameters in rats with streptozotocin (STZ)-induced diabetes were evaluated. The possible protective effect of the F. bengalensis (500 mg/kg of body weight per day) extract on STZ-induced changes was evaluated over 12 weeks. The parameters evaluated were plasma proteins, including total protein albumin and globulin, non-protein nitrogenous substances, including urea, uric acid, and creatinine, and hematological indices, including total hemoglobin content, erythrocytes, leucocytes, and platelets counts. The aqueous extract had a significant (P < 0.05) protective effect against STZ-induced changes in terms of the markers of protein metabolism and hematological indices. Furthermore, increases in levels of non-protein nitrogenous substances were normalized in response to the administration of the extract. Thus, the aqueous extract of F. bengalensis had a significant protective effect on STZ-induced changes to biochemical and hematological parameters in the diabetic rats.

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Identification Of Lactococcus Lactis Subsp. Isolates Based On Their Cell Wall Protein Profiles And Plasmid Contents, Mehtap Üstün, Banu Özden, Ömer Şi̇mşek, Mustafa Akçeli̇k Jan 2009

Identification Of Lactococcus Lactis Subsp. Isolates Based On Their Cell Wall Protein Profiles And Plasmid Contents, Mehtap Üstün, Banu Özden, Ömer Şi̇mşek, Mustafa Akçeli̇k

Turkish Journal of Biology

In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization. According to the cell wall protein patterns, 8.6, 12.5, 14.7, 20.3, 23.4, 25.4, and 31.8 kDa proteins were found identical within 82% of isolates. Moreover 93% of L. lactis subsp. cremoris strains contained a unique 157.8 kDa protein; however, L. lactis subsp. lactis and L. lactis subsp. lactis biovar. diacetylactis had no unique protein in their groups. Also a key plasmid profile, which would be used …

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Hypoxia Induced Changes In The Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, And Antioxidative Response Systems In Zea Mays Leaves, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare Jan 2009

Hypoxia Induced Changes In The Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, And Antioxidative Response Systems In Zea Mays Leaves, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare

Turkish Journal of Biology

The effects of hypoxia on the activities of some enzymes of antioxidative, non- enzymatic scavenging system, membrane permeability, lipid peroxidation, and some reactive oxygen species (ROS) in leaves of Zea mays were investigated. Samples were taken 48, 96, 144, and 192 h after the start of hypoxia treatment. A 192 h hypoxia treatment resulted in a significant rise in membrane permeability, lipid peroxidation (malondialdehyde level), and the production of hydrogen peroxide (H_2O_2) and superoxide (O_2^-) in maize leaves. A short duration of hypoxia enhanced the activity of superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC …

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Antisalmonella Activity Of Selected Medicinal Plants, Chand Pasha, Shaik Sayeed, Sadath Ali, Ziaullah Khan Jan 2009

Antisalmonella Activity Of Selected Medicinal Plants, Chand Pasha, Shaik Sayeed, Sadath Ali, Ziaullah Khan

Turkish Journal of Biology

Antisalmonella activity of some important plants in the Ayurvedic system of traditional medicine used in India to treat enteric diseases was screened. The aqueous and methanol extracts of 47 medicinal plants were studied for their antibacterial activity against pathogenic Salmonella typhi, Salmonella paratyphi A, and Salmonella typhimurium. The in vitro antibacterial activity was performed by agar well diffusion method and the results are expressed as the average diameter of zone of inhibition of bacterial growth around the well. Of the herbal extracts tested, 33 plant extracts were found to have antibacterial activity against at least one of the salmonella strains …

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Antibacterial Activity Of Seed Extracts Of Commercial And Wild Lathyrus Species, Noor Afshan Khan, Sadaf Quereshi, Akhilesh Pandey, Ashutosh Srivastava Jan 2009

Antibacterial Activity Of Seed Extracts Of Commercial And Wild Lathyrus Species, Noor Afshan Khan, Sadaf Quereshi, Akhilesh Pandey, Ashutosh Srivastava

Turkish Journal of Biology

Butanolic extract of seeds from 2 different Lathyrus species (Lathyrus ratan [commercial variety] and L. aphaca [wild variety]), were investigated for their in vitro antibacterial activity. The antibacterial activity against plant and human pathogenic bacteria was evaluated on the basis of inhibition zones (IZs) measured by disc diffusion method. The maximum inhibition was shown by L. ratan against the gram-positive bacterium Staphylococcus aureus. L. aphaca seeds also showed inhibition but were inactive against Klebsiella pneumoniae. L. ratan extract was more active than L. aphaca. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for both the seeds (in …

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The Toxicity And Teratogenicity Of Gibberellic Acid (Ga3) Based On The Frog Embryo Teratogenesis Assay-Xenopus (Fetax), Ayper Boğa, Seçi̇l Bi̇nokay, Yaşar Sertdemi̇r Jan 2009

The Toxicity And Teratogenicity Of Gibberellic Acid (Ga3) Based On The Frog Embryo Teratogenesis Assay-Xenopus (Fetax), Ayper Boğa, Seçi̇l Bi̇nokay, Yaşar Sertdemi̇r

Turkish Journal of Biology

This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA_3), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC_{50}), malformation (EC_{50}), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC_{50} and EC_{50} values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA_3 was 1.69. Different anomalies occurred in the …

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The Effect Of Interaction Of Rauwolfia Vomitoria Root Bark Extract With Vitamin E On Rats’ Liver Enzymes, Monday I. Akpanabiatu, Friday E. Uboh, Theresa B. Ekanem, Imeh Ben Umoh, Eyong Ubana Eyong, Sylvanus O. Ukafia Jan 2009

The Effect Of Interaction Of Rauwolfia Vomitoria Root Bark Extract With Vitamin E On Rats’ Liver Enzymes, Monday I. Akpanabiatu, Friday E. Uboh, Theresa B. Ekanem, Imeh Ben Umoh, Eyong Ubana Eyong, Sylvanus O. Ukafia

Turkish Journal of Biology

In our previous paper we demonstrated the influence of Rauwolfia vomitoria root bark extract on cardiac enzymes of normal Wistar rats and the effectiveness of this root bark as a source of antihypertensive agent, as determined by the marker enzymes, would be a useful index for the toxicity of this plant in clinical application. In this paper we present the results of the effect of Rauwolfia vomitoria root bark extract and its interaction with vitamin E on the liver function enzymes, such as alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate amino transferase (AST), gamma glutamyl transferase (\gamma-GT), and the …

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