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Cyp704b1 Is A Long-Chain Fatty Acid V-Hydroxylase Essential For Sporopollenin Synthesis In Pollen Of Arabidopsis, Anna A. Dobritsa, Jay Shrestha, Marc Morant, Franck Pinot, Michiyo Matsuno, Rob Swanson, Birger Lindberg Møller, Daphne Preuss Jan 2009

Cyp704b1 Is A Long-Chain Fatty Acid V-Hydroxylase Essential For Sporopollenin Synthesis In Pollen Of Arabidopsis, Anna A. Dobritsa, Jay Shrestha, Marc Morant, Franck Pinot, Michiyo Matsuno, Rob Swanson, Birger Lindberg Møller, Daphne Preuss

Biology Faculty Publications

Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be itsmonomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood.Based on a phenotypic mutant screen in Arabidopsis (Arabidopsis thaliana), we identified a cytochrome P450, designatedCYP704B1, as being essential for exine development. CYP704B1 is expressed in the developing anthers. Mutations in CYP704B1result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebraphenotype. Heterologous expression of CYP704B1 in yeast cells demonstrated that it catalyzes v-hydroxylation of long-chainfatty acids, …

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Optimal (Plastic) Life Histories In Growing Versus Stable Populations, Eric Charnov Jan 2009

Optimal (Plastic) Life Histories In Growing Versus Stable Populations, Eric Charnov

Biology Faculty & Staff Publications

No abstract provided.

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Compensatory Evolution Of Gene Regulation In Response To Stress By Escherichia Coli Lacking Rpos, Daniel M. Stoebel, Karsten Hokamp, Michael S. Last, Charles J. Dorman Jan 2009

Compensatory Evolution Of Gene Regulation In Response To Stress By Escherichia Coli Lacking Rpos, Daniel M. Stoebel, Karsten Hokamp, Michael S. Last, Charles J. Dorman

All HMC Faculty Publications and Research

The RpoS sigma factor protein of Escherichia coli RNA polymerase is the master transcriptional regulator of physiological responses to a variety of stresses. This stress response comes at the expense of scavenging for scarce resources, causing a trade-off between stress tolerance and nutrient acquisition. This trade-off favors non-functional rpoS alleles in nutrient-poor environments. We used experimental evolution to explore how natural selection modifies the regulatory network of strains lacking RpoS when they evolve in an osmotically stressful environment. We found that strains lacking RpoS adapt less variably, in terms of both fitness increase and changes in patterns of transcription, than …

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Global Chloroplast Phylogeny And Biogeography Of Bracken (Pteridium: Dennstaedtiaceae), Joshua P. Der, J. A. Thomson, J. K. Stratford, Paul G. Wolf Jan 2009

Global Chloroplast Phylogeny And Biogeography Of Bracken (Pteridium: Dennstaedtiaceae), Joshua P. Der, J. A. Thomson, J. K. Stratford, Paul G. Wolf

Biology Faculty Publications

Bracken ferns (genus Pteridium ) represent an ancient species complex with a natural worldwide distribution. Pteridium has historically been treated as comprising a single species, but recent treatments have recognized several related species. Phenotypic plasticity, geographically structured morphological variation, and geographically biased sampling have all contributed to taxonomic confusion in the genus. We sampled bracken specimens worldwide and used variable regions of the chloroplast genome to investigate phylogeography and reticulate evolution within the genus. Our results distinguish two major clades within Pteridium , a primarily northern hemisphere Laurasian/African clade, which includes all taxa currently assigned to P. aquilinum , and …

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Ecomorphology Of The Mexican Fence Lizards Of The Sceloporus Formosus Group (Squamata: Phrynosomatidae), Gloria Andrea Acevedo Jan 2009

Ecomorphology Of The Mexican Fence Lizards Of The Sceloporus Formosus Group (Squamata: Phrynosomatidae), Gloria Andrea Acevedo

Biology Theses - Archive

A central belief of ecomorphological studies is the link between morphology and performance, but, surprisingly few analyses demonstrate this relationship. Fence lizards of the genus Sceloporus offer a useful system for addressing correlations between morphology and performance because they possess keratinized and keeled scales, a distinctive morphological character that might influence water balance. A common observation across different taxa of reptiles is that many organisms in dry habitats have few scales on their bodies, compared to organisms inhabiting moist environments. This pattern is also present in fence lizards. I investigated if the number of dorsal and lateral scales varies across …

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Analysis Of Novel Regulatory Region And Function Of A Young Drosophila Retrogene: Dntf-2r, Mansi Motiwale Kunte Jan 2009

Analysis Of Novel Regulatory Region And Function Of A Young Drosophila Retrogene: Dntf-2r, Mansi Motiwale Kunte

Biology Dissertations - Archive

Dntf-2r is a young retroposed gene that originated from the nuclear transport gene Dntf-2. Here the function, quality and evolutionary origin of the regulation of this retrogene are explored. In Chapter 1, I introduce the background of what is known of Dntf-2r as well as the function of its parental gene. This includes their evolutionary histories, and known functional and regulatory aspects. Chapter 2 describes my work on the novel regulatory region recruited by the retrogene and I explore the tissue specific expression pattern (i.e. testis expression) driven by this region. In Chapter 3 I set myself up to review …

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Thermal Tolerance, Physiologic Condition, And Population Genetics Of Dreissenid Mussels (Dreissena Polymorpha And D. Rostriformis Bugensis) Relative To Their Invasion Of Waters In The Western United States, John T. Morse Jan 2009

Thermal Tolerance, Physiologic Condition, And Population Genetics Of Dreissenid Mussels (Dreissena Polymorpha And D. Rostriformis Bugensis) Relative To Their Invasion Of Waters In The Western United States, John T. Morse

Biology Dissertations - Archive

Zebra mussels, Dreissena polymorpha, and quagga mussels, D. rostriformis bugensis, Eurasian freshwater bivalves, invaded the North American (NA) Great Lakes in the mid 1980's. Subsequently, they invaded water bodies east of the Rocky Mountains. Their more recent expansion into warm, isolated, southwestern U.S. water bodies suggests that both species are developing thermally-tolerant physiological races. Chronic upper thermal tolerance limits were determined for D. polymorpha populations from Winfield City Lake (KS) (WCL), Lake Oologah (OK) (OL) and Hedges Lake (NY) (HL), and the D. rostriformis bugensis population from Lake Mead (NV/AZ) (LM). Individuals from WCL had 12-d and 28-d incipient upper …

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Sex, War And Disease: The Effects Of Infection On Horn Size And Intra-Sexual Competition In The Broad-Horned Flour Beetle, Gnathocerus Cornutus, Amrita Naidu Jan 2009

Sex, War And Disease: The Effects Of Infection On Horn Size And Intra-Sexual Competition In The Broad-Horned Flour Beetle, Gnathocerus Cornutus, Amrita Naidu

Biology Theses - Archive

Sexual selection is widely used to explain the evolution of mating systems where most often it is manifest as the competition among males for access to females. This competition takes the form of direct male-male interactions in broad-horned flour beetle Gnathocerus cornutus and often results in exaggerated male phenotypes. Sexually selected traits are interesting because in many cases their existence appears to contradict natural selection. For example, the "handicap hypothesis" suggests that there may be a trade-off between immune response and horn size because mounting an immune response necessary for survival (natural selection) may divert resources away from growing longer …

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Determining The Phylogeographic Dynamics Of The Edge Relationships Between Aphonopelma Hentzi (Girard) And Its Neighbors Along The Colorado River Basin (Araneae, Mygalomorphae, Theraphosidae), Chris A. Hamilton Jan 2009

Determining The Phylogeographic Dynamics Of The Edge Relationships Between Aphonopelma Hentzi (Girard) And Its Neighbors Along The Colorado River Basin (Araneae, Mygalomorphae, Theraphosidae), Chris A. Hamilton

Biology Theses - Archive

Theraphosids, commonly known as “tarantulas”, are a group of large, hirsute spiders that have drawn relatively little interest in ecological, behavioral, or evolutionary research from biologists. The lack of research on theraphosids is problematic because of conservation concerns for this group and the need to compile good life history data, and determine taxonomic and phylogenetic relationships. I examined the phylogenetic relationships among populations of tarantulas of the genus Aphonopelma along the Colorado River basin in Texas to construct a framework within which to test ecological, evolutionary, and biogeographical hypotheses in an area of species transition and possible Pleistocene refugia. In …

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Assessment Of Biology Majors’ Versus Nonmajors’ Views On Evolution, Creationism, And Intelligent Design, Guillermo Paz-Y-Mino C., Avelina Espinosa Jan 2009

Assessment Of Biology Majors’ Versus Nonmajors’ Views On Evolution, Creationism, And Intelligent Design, Guillermo Paz-Y-Mino C., Avelina Espinosa

Arts & Sciences Faculty Publications

The controversy around evolution, creationism, and intelligent design resides in a historical struggle between scientific knowledge and popular belief. Four hundred seventy-six students (biology majors n=237, nonmajors n=239) at a secular liberal arts private university in Northeastern United States responded to a five-question survey to assess their views about: (1) evolution, creationism, and intelligent design in the science class; (2) students’ attitudes toward evolution; (3) students’ position about the teaching of human evolution; (4) evolution in science exams; and (5) students’ willingness to discuss evolution openly. There were 60.6% of biology majors and 42% of nonmajors supported the exclusive teaching …

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The Mckillop Library Waste Stream, Kalyna Macko, Kelsy Patnaude, Other Students Jan 2009

The Mckillop Library Waste Stream, Kalyna Macko, Kelsy Patnaude, Other Students

BIO140 Carbon Footprint Project

The McKillop Library's resources come at an environmental cost because more resources are being used than is realized. The focus is on the amount of carbon emissions and waste that is being produced by the library. Carbon emissions are the leading cause of climate change, and through recycling the library can lower the amount of carbon the library is producing. If recycling and re-using materials becames more of a common procedure in library, there is little doubt that it would be more environmentally friendly.

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Effects Of Temperature On Reproduction And Survival Of The Calanoid Copepod Pseudodiaptomus Pelagicus, Andrew L. Rhyne, Cortney L. Ohs, Erik Stenn Jan 2009

Effects Of Temperature On Reproduction And Survival Of The Calanoid Copepod Pseudodiaptomus Pelagicus, Andrew L. Rhyne, Cortney L. Ohs, Erik Stenn

Arts & Sciences Faculty Publications

Four experiments were conducted on the calanoid copepod, Pseudodiaptomus pelagicus, to determine the effects of temperature (24, 26, 28, 30, 32, and 34 °C) on survival, development time, reproductive output, and population growth in order to define the optimal temperature for culture. The first experiment stocked early stage nauplii into 1 L beakers and cultured them using standard procedures until five days after the first mature adults were observed; from this survival, sex ratio, time to maturation, and fecundity were measured. The second and third experiments evaluated the effects of temperature on nauplii production by stocking individual pairs and …

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In Situ Manipulation Of Vertically Migrating Gelatinous Zooplankton Using Nighttime Blue-Water Scuba In The South-Central Adriatic Sea, William M. Graham, John H. Costello, Sean Colin, Alenka Malej, Davor Lučić, Vladimir Onofri, Adam Benović Jan 2009

In Situ Manipulation Of Vertically Migrating Gelatinous Zooplankton Using Nighttime Blue-Water Scuba In The South-Central Adriatic Sea, William M. Graham, John H. Costello, Sean Colin, Alenka Malej, Davor Lučić, Vladimir Onofri, Adam Benović

Arts & Sciences Faculty Publications

Technological advance in undersea exploration (e.g. tethered cameras, remotely operated vehicles [ROVs], Autonomous Underwater Vehicles [AUVsG, and manned submersibles)have opened new windows into diversityand distribution of fragile gelatinous organisms in the vast mesopelagic realm(300 m-1000 m deep). While exstraordinary in expanding our view of its richness, mesopelagic exploration remains largely a "look but don't touch" environment and this limits of our ability to understand these animals through physical manipulation relevant to the finer scales of the individual organism. We have been conducting a series of in situ observations and manipulations using blue-water SCUBA during the night at a 1, 200 …

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In Vitro Inhibition By Aerosol-Ot Of Bacillus Anthracis Sterne Endospores, Andrea M. Rendziperis Jan 2009

In Vitro Inhibition By Aerosol-Ot Of Bacillus Anthracis Sterne Endospores, Andrea M. Rendziperis

Senior Honors Theses and Projects

Bacillus anthracis is an endospore-forming, Gram-positive bacterium that is the causative agent of the disease anthrax. The sodium ion surfactant Aerosol-OT (AOT) has been shown to kill some species of Gram-positive bacteria. This study examined the effect of AOT on B. anthracis endospores and vegetative cells, to show any changes in germination and growth that could potentially affect host development of disease. B. anthracis endospores and vegetative cell cultures were exposed to varying levels of AOT and samples were heated to show the proportion of endospores, or washed of the supernatant to show any temporary inhibitory effects on growth. B. …

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Species Richness And Host Associations Of Lepidoptera-Attacking Tachinidae In The Northeast Ecuadorian Andes, John O. Stireman Iii, Harold F. Greeney, Lee A. Dyer Jan 2009

Species Richness And Host Associations Of Lepidoptera-Attacking Tachinidae In The Northeast Ecuadorian Andes, John O. Stireman Iii, Harold F. Greeney, Lee A. Dyer

Biological Sciences Faculty Publications

Most of the unknown biological diversity of macro-organisms remaining to be discovered and described lies in the tropical regions of the world and consists primarily of insects. Those insects with parasitoid lifestyles constitute a significant portion of insect diversity, yet parasitoids are among the most poorly known of major insect guilds in the humid tropics. Here we describe and analyze the richness of one diverse taxon of parasitoids, flies in the family Tachinidae, reared from Lepidoptera as part of a biological survey of Lepidoptera and their parasitoids in one mid-elevation (2000 m) area in the northeast Ecuadorian Andes. One hundred …

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Habitat Variation Among Aquatic Gastropod Assemblages Of Indiana, U.S.A., Mark Pyron, Jayson Beugly, Matthew Spielman, Jennifer Pritchett, Stephen J. Jacquemin Jan 2009

Habitat Variation Among Aquatic Gastropod Assemblages Of Indiana, U.S.A., Mark Pyron, Jayson Beugly, Matthew Spielman, Jennifer Pritchett, Stephen J. Jacquemin

Biological Sciences Faculty Publications

We collected aquatic gastropods at 137 sites in lakes and streams of Indiana and tested for patterns of assemblages with environmental variables. The survey resulted in 32 species with a mean of 2.8 species at each site, and a mean abundance at each site of 144 individuals. Nonmetric multidimensional scaling (NMS) multivariate analyses resulted in watershed drainage area, water conductivity, substrate category frequency, and dissolved oxygen as significant correlates of gastropod assemblage structure. Gastropod assemblages of lakes were not significantly different than assemblages of streams in the ordination. Prosobranch taxa occurred in higher abundances than pulmonate taxa at sites with …

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Habitat Use By The Southeastern Beach Mouse (Peromyscus Polionotus Niveiventris) At Cape Canaveral Air Force Station, Florida, Kathryn Simmons Jan 2009

Habitat Use By The Southeastern Beach Mouse (Peromyscus Polionotus Niveiventris) At Cape Canaveral Air Force Station, Florida, Kathryn Simmons

Electronic Theses and Dissertations

Successful recovery of the federally threatened southeastern beach mouse (Peromyscus polionotus niveiventris) depends in part on an understanding of their habitat requirements. I studied habitat use by beach mice at Cape Canaveral Air Force Station, Florida from March 2005 until March 2006. I livetrapped six grids, three on coastal dunes and three within scrub located inland from the coast. On each grid and trap station, I quantified the extent of bare ground, woody vegetation, non-woody vegetation, height of vegetation, and percentage of coarse sand in the surface soil. I assessed trap success relative to these habitat variables using linear and …

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Sequence Evolution Of Recurrently Recruited Retroposed Genes In Drosophila And Their Possible Role In Meiotic Drive, Charles David Tracy Jan 2009

Sequence Evolution Of Recurrently Recruited Retroposed Genes In Drosophila And Their Possible Role In Meiotic Drive, Charles David Tracy

Biology Theses - Archive

Gene duplications are a valuable source of genetic information that can evolve under positive selection creating a new gene function without affecting the original function.A gene duplication mechanism is retroposition. Retroposed copies of genes (retrogenes) are created by reverse transcription of a mRNA into the host organism's genome producing a new sequence that has the same protein coding capacity as the parental gene but lacks introns and regulatory regions. Ran and Dntf-2 are genes involved in nuclear transport that have given rise to retrogenes three times in the Drosophila genus.Recently, genes involved in nuclear transport such as Ran and Dntf-2 …

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Hispanic Medicinal Herbal Remedies And Cultural Competency: An Examination Of Pre-Hispanic And Hispanic Medicinal Plants And The Need For Physicians In Los Angeles To Know These Plants As One Basis For Cultural Competency, Garik N. Linnastruth Jan 2009

Hispanic Medicinal Herbal Remedies And Cultural Competency: An Examination Of Pre-Hispanic And Hispanic Medicinal Plants And The Need For Physicians In Los Angeles To Know These Plants As One Basis For Cultural Competency, Garik N. Linnastruth

Whittier Scholars Program

The demographic majority of the people who reside in Los Angeles are hispanic. This population often utilizes alternative medicine, particularly herbal remedies, to ameliorate their health problems. As such, what herbal medicinal plants do physicians in Los Angeles County need to have background knowledge about to be considered culturally competent? Does the knowledge of medicinal plants dictate one basis for cultural competency? The purpose of this study is to examine ten Latino alternative/folk medicinal remedies from three separate civilizations to identify key ways in which physicians can increase their cultural competency. The first two civilizations are Pre-Hispanic and involve the …

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Host And Seasonal Effects On The Infection Dynamics Of Skrjabinoptera Phrynosoma (Ortlepp) Schulz, 1927, A Parasitic Nematode Of Horned Lizards, Kathryn Claire Hilsinger Jan 2009

Host And Seasonal Effects On The Infection Dynamics Of Skrjabinoptera Phrynosoma (Ortlepp) Schulz, 1927, A Parasitic Nematode Of Horned Lizards, Kathryn Claire Hilsinger

College of Graduate Studies: Theses & Dissertations

Author's Abstract: Skrjabinoptera phrynosoma (Ortlepp) Schulz, 1927 is a common parasitic nematode of horned lizards. The life cycle of S. phrynosoma was described by Lee in 1957, but has received little attention since. The present study addressed effect of season as well as host characteristics on the infection dynamics in lizard hosts. In the Alvord Basin in southeastern Oregon, S. phrynosoma were collected from Phrynosoma platyrhinos Gerard 1852 horned lizards via stomach flushes, cloaca flushes and fecal pellet collections. Parasite load variables (number of nematodes per host, length of those nematodes, and total worm burden (ΣL)) were analyzed within three …

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The Effect Of Glutamate On Neurite Outgrowth In Fiddler Crab (Uca Pugilator) X-Organ Cells, Ruth B. Langton Jan 2009

The Effect Of Glutamate On Neurite Outgrowth In Fiddler Crab (Uca Pugilator) X-Organ Cells, Ruth B. Langton

Honors Theses

Glutamate is the primary excitatory neurotransmitter in the mammalian central nervous system. It is of particular interest because of its supposed role in the processes of learning and memory, and also because of its potential toxic effects that have been linked to neurodegenerative diseases, such as Alzheimer’s and Parkinson’s. Although glutamate is necessary for normal cell functioning, high levels of glutamate receptor activation can result in cell death, a phenomenon known as excitotoxicity. It has been suggested that glutamate also plays an important role in the insect and crustacean nervous systems, allowing for the examination of excitotoxicity in these organisms. …

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The Effect Of Coalmine Effluent On The Parasite Assemblages Of Lepomis Spp. In The Saline River Basin, Andrew Claxton Jan 2009

The Effect Of Coalmine Effluent On The Parasite Assemblages Of Lepomis Spp. In The Saline River Basin, Andrew Claxton

Masters Theses

To determine the efficacy of using parasite assemblages as bioindicators of coalmine effluent, a survey of gastro-intestinal helminths from the gut of sunfish, Lepomis spp, was performed. Five species of Lepomis, L. cyanellus, L. nwcrochirus, L. megalotis, L. microlophus, and L. gulosus, were collected from six locations near the Palzo mine, an abandoned coalmine that actively discharges into the lower reach of Sugar Creek. Sunfish were collected from three sites which did not receive effluent from the Palzo mine (Sugar Creek (37° 37'/ 88° 50'), South Fork(37° 41 '/ 88° 52'), and the Little Saline River (37° 38'/ 88° 38')) …

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Feathers As Bioindicators Of Pcb Exposure In Clapper Rails, Jay W. Summers Jan 2009

Feathers As Bioindicators Of Pcb Exposure In Clapper Rails, Jay W. Summers

Masters Theses

In this study we used feathers as a biomonitor for exposure to the polychlorinated biphenyl (PCB) Aroclor 1268. Clapper rails have been used as an indicator species of environmental damage for the LCP superfund site located in Brunswick, GA USA. LCP is contaminated with Aroclor 1268, which has been used in limited amounts elsewhere and therefore can be used as a contaminant marker. Aroclor 1268, including congener profiles, were quantified using gas chromatography (GC). Concurrently each sample was quantified for total Aroclor 1268 using an enzyme-linked immunosorbant assay (ELISA) and compared to the GC results to determine if ELISA was …

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Behavioral Response Of Japanese Beetles (Popillia Japonica) To Sex Pheromone: Exploring Factors Of Social Situation And Recent Mating Experience, Carissa A. Schoenick Jan 2009

Behavioral Response Of Japanese Beetles (Popillia Japonica) To Sex Pheromone: Exploring Factors Of Social Situation And Recent Mating Experience, Carissa A. Schoenick

Masters Theses

Insects in have several methods of advertising for mates; one important and common method is via the use of chemical pheromones. Insects release pheromones into the environment to communicate information about their sex, mating status and receptivity to others. For example, Japanese beetles (Popillia japonica) produce a very volatile sex pheromone conspecifics can readily detect. This pheromone is released by virgin females only, and triggers scramble competition among the males that detect and follow the signal for access to these females. Because Japanese beetles are an invasive species and an important pest of ornamental and agricultural plants, there is strong …

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Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii Jan 2009

Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii

Masters Theses

The Illinois Environmental Protection Agency (IEPA) and U.S. Environmental Protection Agency (USEP A) identify needs to monitor aquatic resources by quantitative means using biocriteria in conjunction with routine environmental assessment to identify sources of impairment, and determine the effectiveness of management protocols (IEP A 2007; USEPA 2007). Though bioassessment protocols have been implemented within lotic systems and lakes of natural origin, investigation of their application to functionally unique reservoir habitat, which dominates the Midwest, has been limited. This study .evaluated the utility of photopigment profiles, attained using high-performance liquid chromatography (HPLC), for development ofbioassessment protocols for Illinois lakes and reservoirs. …

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Reintroduction Of Bison (Bison Bison) On Reservations In South Dakota: Four Case Studies, Trudy M. Ecoffey Jan 2009

Reintroduction Of Bison (Bison Bison) On Reservations In South Dakota: Four Case Studies, Trudy M. Ecoffey

Electronic Theses and Dissertations

This research explored and documented issues of sustainability associated with the reintroduction of bison in four different bison herds on Reservations in South Dakota. The four herds were managed by: a tribal family cooperative, an individual tribal member, a tribal university and a tribal fish and wildlife agency. The objectives were to identify management practices associated with the sustainability of bison introduction by American Indians; to explore the role of American Indian culture in the management of bison for sustainability; and to investigate rangeland criteria that could be used to measure sustainability of bison reintroduced by American Indians. This project …

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Mojave Applied Ecology Notes Winter 2009, Scott R. Abella, Lindsay P. Chiquoine, Alice C. Newton Jan 2009

Mojave Applied Ecology Notes Winter 2009, Scott R. Abella, Lindsay P. Chiquoine, Alice C. Newton

Mojave Applied Ecology Notes

Chronology of forest structure and use in the Spring Mountains, Soil-Tech’s restoration work balancing construction with nature, species performance and treatment effectiveness for revegetation projects, and strategic research areas for Mojave conservation and management

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Comparison Of Zeocin And G418 Resistance Markers In Pichia Pastoris, Allison Moy Jan 2009

Comparison Of Zeocin And G418 Resistance Markers In Pichia Pastoris, Allison Moy

University of the Pacific Theses and Dissertations

The methylotrophic yeast, Pichia pastoris, has been used to successfully produce and express over 700 heterologous proteins. Currently, the only two dominant selectable markers available are the Zeocin and blasticidin resistance genes. This study focused on characterizing and developing the modified G418 selectable marker so it could be used for primary selection of single copy and multicopy strains. To demonstrate its use as a selectable marker the SLPI reporter gene was inserted into the Zeocin resistance vector, pPICZB, and the new G418 resistance vector, pKANa B. The resulting constructs were then transformed into P. pastoris yJC100 cells. Piggyback strains containing …

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Beheading First: On Nabokov's Translation Of Lewis Carroll, Victor Fet Jan 2009

Beheading First: On Nabokov's Translation Of Lewis Carroll, Victor Fet

Biological Sciences Faculty Research

Anya v Strane chudes, young Nabokov’s 1923 Russian translation of Alice’s Adventures in Wonderland, contains an intentionally shifted statement “beheading first, sentence later” compared to Lewis Carroll’s “sentence first, verdict later”. The shift is fitting for the 1920s children émigré audience.

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Charting The Late Cretaceous Seas: Mosasaur Richness And Morphological Diversification, Marcus R. Ross Jan 2009

Charting The Late Cretaceous Seas: Mosasaur Richness And Morphological Diversification, Marcus R. Ross

Faculty Publications and Presentations

Abundant, readily identifiable, and biostratigraphically resolved specimens make mosasaurs ideal candidates to test fluxes in generic richness and morphological trends among marine vertebrates during the late Cretaceous. More than 1800 globally distributed mosasaur specimens are allocated to fifteen substage-level stratigraphically correlated assemblages. These data are quantitatively analyzed to illuminate trends within the group. Following their first appearance in the Cenomanian, mosasaurs experienced a significant radiation in the Coniacian and Santonian. Richness levels continued increasing into the Maastrichtian while mosasaurs incrementally exploited new predatory niches recognized via accumulation of novel dental morphologies. Their extinction at the end of the Cretaceous occurred …

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