Digital Commons Network^™

Alfred Russel Wallace Notes 33. The Story In Evolution., Charles H. Smith

Faculty/Staff Personal Papers

Alfred Russel Wallace (1823-1913) is best known for his independent formulation of the principle of natural selection. In this work I first review my understanding of Wallace’s intellectual development on this subject, including the notion that he had no model for the evolution of higher consciousness as of his first treatment of natural selection in 1858. This leads to some conclusions about his approach to the subject, and links to parallel ideas inherent in the philosophy of Baruch de Spinoza (1632-1677).

Environmental Change As A Driver Of The Evolution Of Migratory Behavior In Suboscine Birds, Emily Johns

Open Access Theses & Dissertations

Avian migration, particularly long-distance seasonal migratory movement, has long captured the attention of researchers and the public alike. However, the evolutionary origins of migratory behavior are poorly understood, in part due to limited understanding of the full range of migratory strategies including short-distance and intra-tropical movements. We examine the climatic drivers of migration across suboscine birds, a group of roughly 1,300 primarily Neotropical species with a complex array of migratory strategies. Our reconstructions of the evolutionary history of suboscine migration reveal that migratory behavior is labile and is lost approximately twice as often as it is gained. We find that …

Implementing The Methodology To Generate Diagnostic And Therapeutic Antibodies, Andrea Mariana Carlin

Open Access Theses & Dissertations

This research aimed to establish the methodology for developing monoclonal antibodies at The University of Texas at El Paso. Monoclonal antibodies are indispensable across multiple fields, including diagnostics, therapeutics, and biotechnology, yet there is a shortage of commercially available antibodies targeting specific proteins or possessing specialized characteristics. To address this gap, three models were selected: a peptide derived from the intracellular protein Schlafen 8 and two cell surface protein receptors: mouse vascular endothelial growth factor receptor 2 (VEGFR2) and human melanocortin receptor 4 (MC4R). These proteins present unique challenges in antibody generation, including issues of accessibility, expression, and specificity.

Throughout …

Evolutionary History Of A Specialized And Diverse Highland Snake Complex (Crotalus Willardi), Michael Joseph Buontempo

Open Access Theses & Dissertations

The Ridgenosed rattlesnake (Crotalus willardi) is a highly unique group of small rattlesnakes consisting of 5 subspecies that inhabit the high elevation pine oak woodlands of the Sierra Madre Occidental and the Madrean Archipelago. They remain one of the most understudied groups of rattlesnakes with little understanding of the relationships between the subspecies. I utilize genomic level genetic data collected using ddRAD-seq methodology on 60 individuals encompassing all 5 subspecies and the majority of the range. Utilizing co-ancestry analysis, Admixture, and Phylogenetic analyses (both maximum likelihood and Bayesian approaches) we uncovered seven separate genetic groups. Of these, three consisted of …

Preparing Educators To Effectively Engage With Postsecondary Stem Students With Autism, Luzconsuelo Gavaldon

Open Access Theses & Dissertations

Autism spectrum disorder (ASD) is a lifetime mental disability (Boucher, 2009). Because the brains of people with autism work differently than their non-neurodiverse peers (Kuzminskaite et al., 2020), they tend to respond to external stimuli in a hypo- or hyper-sensory fashion. One of the primary considerations that must be accounted for when working with individuals with autism is that the brains of these persons are each neurologically distinct. In other words, there is no "one-size-fits-all" means by which individuals with ASD learn and develop and, consequently, no single way to therefore engage with said individuals (Anderson & Butt, 2017; Kuzminski …

Heat Acclimated Algae Improves Heat Tolerance And Longevity In Daphnia, Ashit Dutta

Electronic Theses and Dissertations

Xenohormesis suggests that consuming stressed food provides physiological adaptation to the consumer for similar stresses. To evaluate this, Daphnia magna was provided with heat-stressed Nannochloropsis limnetica grown at 25℃ whereas the control group was provided Nannochloropsis limnetica grown at 12℃. In the heat tolerance experiment, Daphnia kept at 28℃ and 20℃ while consuming 25℃ algae had significantly higher heat tolerance compared to the control. Additionally, in stressed conditions, Daphnia consuming 25℃-grown food had significantly higher lifespan compared to the control. Next, Daphnia consuming 25℃-grown algae had significantly higher levels of antioxidants which could explain the lifespan extension. Finally, in fatty …

Patterns In Gray Wolf Pup Survival And Recruitment In Northern Minnesota, Andrea Hynes

All NMU Master's Theses

Knowledge of how and why the vital rates of wildlife populations change through and over time is crucial for understanding wildlife population dynamics. Because estimating gray wolf pup survival is challenging, our understanding of pup survival and the factors influencing survival and recruitment is limited. I used a combination of litter counts and video observations from a large remote camera array to estimate annual wolf pup survival and recruitment and to evaluate intra-annual patterns in pup survival and recruitment within the Greater Voyageurs Ecosystem, MN, USA. Mean annual pup survival during 2019–2023 was 0.24, and the mean annual recruitment rate …

Effects Of Metoprolol And Propranolol Beta-Blockers Mixture On Morphological And Physiological Responses In The American Oyster, Andrew Salinas

Theses and Dissertations

Beta-blockers are extensively used to treat human cardiovascular conditions yet pose significant risks to non-target aquatic organisms. This study aimed to investigate the effects of short-term exposure (one week) to a beta-blocker’s mixture (low-dose: 50 ng/L propranolol and 250 ng/L metoprolol, and high-dose: 250 ng/L propranolol and 650 ng/L metoprolol) in the American oysters. Histopathological assessments revealed structural damage to gills, connective tissues, and digestive glands in both low- and high-dose treatment groups. Additionally, glucose concentration and pH of the extrapallial fluid significantly declined in the high-dose treatment group. Hemocyte density within connective tissues increased proportionally with dosage. The beta-blocker …

Development Of An In Vitro Squamous Metaplasia Model Using Airway Basal Stem Cells, Arash Tahmasbi

Electronic Theses, Projects, and Dissertations

Squamous metaplasia (SM) of the airway epithelium is an abnormal condition that is caused by exposure to airborne toxicants, cigarette smoke, pollutants, and pathogens. Since SM of the airway was first documented in the 1970s, data on this epithelial disease remain limited. More research is needed to fully understand this epithelial disorder and to prevent its progression into severe illnesses such as chronic bronchitis (CB), COPD, and squamous cell carcinoma. We developed 3D models of SM using airway basal stem cells (ABSCs) isolated from lung obtained from postmortem human donors. The ABSCs were cultured at the air-liquid interface (ALI) for …

Gc-Ms Analysis And Evaluation Of Antioxidant, Anticancer, Cytotoxicity, And Hypoglycemic Properties Of The Libyan Moringa Oleifera Leaf Extract On Alloxan-Induced Diabetes In Mice, Osama Abdel-Hafeez Mohammed Al-Bedak, Ahlam Rashed, Seham T.M. Oskandali, Nouri Kushlaf, Amal Eishebani, Samira A. Alacrouk, Fadia T.M. Oshkandale, Manal Eisheli, Zahur R. Almesai, Ameerah Shaeroun, Steven L. Stephenson, Fuad Ameen

Biological Sciences Faculty Publications and Presentations

Libyan Moringa oleifera leaves were extracted using methanol, and the bioactive components' antibacterial, antioxidant, anti-inflammatory, and anticancer characteristics were assessed using gas chromatography mass spectrometry analysis (GC-MS). Moringa oleifera exhibited antioxidant activity, with an IC50 of 11.53 µg/mL. Wi38 cell line's viability decreased with increasing M. oleifera extract concentration, while its cytotoxicity increased with an IC50 of 393.41±7.77 μg/mL. Alloxan-induced diabetes model in Swiss albino mice was implemented. Three different doses (100, 200, or 300 mg/kg of mice body weight) of M. oleifera’s extract were evaluated for their effects on blood sugar concentration and the histology of the pancreatic islets. …

Evaluating The Stress System Of The Grieving Brain: Corticotropin-Releasing Factors And Partner Loss In Female Coyotes, Rachel Tong Xin Ning

Undergraduate Honors Capstone Projects

Forming social bonds is fundamental in helping us foster connections with others. The loss of a loved one often results in grief, stress, and loneliness, and the stress response system of the body has been implicated in the physiological symptoms associated with grieving. Corticotropin releasing factor (CRF) is the hormone that initiates the stress response in the body and acts at two different receptor subtypes CRF receptor (CRFR)1 and CRFF2. Many studies on CRF and social loss have been conducted in monogamous prairie voles, but studies in longer-lived monogamous mammals could improve understanding of the effects of losing pair bonds. …

Time Series Decomposition Of Land Surface Temperature For Long-Term Trend Forecasting And Impact On Nesting Sea Turtle Habitats In The Arabian Gulf, Sachi Perera, Rommel H. Maneja, Mohamed Allali, Cyril Rakovski, Erik Linstead, Daniele Struppa, Ali Qasem, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Improving land surface temperature (LST) modeling is vital for mitigating climate change effects on various ecosystems and marine habitats such as important sea turtle habitats. Over the past decade, extreme temperatures have likely significantly affected nesting sea turtle habitats in the Arabian Gulf, with predominantly female hatchlings creating an imbalance in the sex ratio. Such shifts have profound implications for these habitats’ long-term survival and conservation management. This study leverages statistical machine learning models to measure ongoing temporal variations in LST. We break down the LST time series into trend, seasonal, and noise components using classical decomposition methods like X11, …

Reducing Dna Extraction Costs Through Factorial Design For The Dnadvance Kit, Carson J. Neal, Zachery D. Zbinden, Michael E. Douglas, Marlis R. Douglas

Biological Sciences Faculty Publications and Presentations

Objective

Extracting DNA is essential in wildlife genetic studies, and numerous methods are available. However, the process is costly and time-consuming for non-model organisms, including most wildlife species. Therefore, we optimized a cost-efficient protocol to extract DNA from the muscle tissue of White-tailed Deer using the DNAdvance kit (Beckman Coulter), a magnetic-bead-based approach. We devised a 3 × 3 factorial design using combinations of tissue mass (10 mg, 50 mg, or 100 mg) and reaction volume (25%, 33%, and 50% of the manufacturer's recommended volumes). DNA was extracted for N = 81 tissue sub-samples (9 replicates/treatment).

Results

Our target yield …

The Effects Of Wildfires On Predation Risk, Perch Selection, And Background-Matching In Western Fence Lizards, Elmer Ernesto Gutierrez

Electronic Theses, Projects, and Dissertations

Fires are a naturally occurring phenomenon, driving a range of behavioral, reproductive, and morphological adaptations in wildlife. However, we are now living in a new geological epoch—the Pyrocene—characterized by human-induced extreme fire behavior. This shift results in a need to understand how animals survive and adapt in post-fire environments. In this study, we investigated the following questions: (1) Does predation risk increase in burned habitats after a fire? (2) How does dorsal darkness morphology vary between burned and unburned habitats? and (3) Do animals select perches that enhance their background-matching in post-fire environments? We used the Western Fence Lizard ( …

Assessing The Influence Of Native And Invasive Grasses On The Behavior Of Aedes Aegypti In South Texas, Emily Medelez

Theses and Dissertations

Invasive grasses in South Texas can have widespread negative effects, displacing native species, reducing biodiversity, and posing risks to public health. These grasses may flourish in moist, shaded environments, which not only support their growth but also creates habitats favorable for mosquito activity. This can increase the population and alter the behavior of mosquitoes, potentially raising the risk of disease transmission. In this thesis, I look at the behavior of mosquito species Aedes aegypti, primary vector of the yellow fever virus. I analyzed the influence of oviposition, flight and resting behaviors. I tested the relative attraction of Aedes mosquitoes …

Size Matters: The Characterization Of Telomere Function In Germ Cell Development In Danio Rerio, Jessica Macneil

Graduate Doctoral Dissertations

Infertility is a growing problem worldwide. According to the WHO, 48 million couples across the globe suffer from infertility and many couples turn to Assisted Reproductive Technology (ART) to become pregnant. One readout of gamete quality and successful ART is telomere integrity because telomeres are necessary for chromosome movements during meiosis so that the segregation of chromosomes happens correctly. Telomeres are well known for their role in aging; however, they play an important and understudied role during meiosis. In meiosis, telomeres attach to the nuclear envelope (NE) via a protein chain spanning the NE that interacts with cytoplasmic motor proteins, …

Genomic Insights Into Key Mechanisms For Carbon, Nitrogen, And Phosphate Assimilation By The Acidophilic, Halotolerant Genus Acidihalobacter Members, Himel Nahreen Khaleque, Homayoun Fathollahzadeh, Anna H. Kaksonen, Jorge Valdés, Eva Vergara, David S. Holmes, Elizabeth L.J. Watkin

Research outputs 2022 to 2026

In-depth comparative genomic analysis was conducted to predict carbon, nitrogen, and phosphate assimilation pathways in the halotolerant, acidophilic genus Acidihalobacter. The study primarily aimed to understand how the metabolic capabilities of each species can determine their roles and effects on the microbial ecology of their unique saline and acidic environments, as well as in their potential application to saline water bioleaching systems. All four genomes encoded the genes for the complete tricarboxylic acid cycle, including 2-oxoglutarate dehydrogenase, a key enzyme absent in obligate chemolithotrophic acidophiles. Genes for a unique carboxysome shell protein, csoS1D, typically found in halotolerant bacteria but not …

Environmental Light Controls The Daily Organization Of Breathing By Activating Brn3b-Expressing Intrinsically Photosensitive Retinal Ganglion Cells In Mice, Aaron A. Jones, Allison R. Spears, Deanna M. Arble

Biological Sciences Faculty Research and Publications

Rhythmic, daily fluctuations in minute ventilation are controlled by the endogenous circadian clock located in the suprachiasmatic nucleus (SCN). While light serves as a potent synchronizer for the SCN, it also influences physiology and behavior by activating Brn3b-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs). It is currently unclear the extent to which the external light environment shapes daily ventilatory patterns independent of the SCN. To determine the relative influence of environmental light versus circadian timing on the organization of daily rhythms in minute ventilation, we used whole-body plethysmography to measure the breathing of mice housed on a non-entraining T28 cycle …

Geomorphic Assessment And Flow Modeling Of Pearl Darter (Percina Aurora) Habitat In The Pascagoula River Watershed, Malia Davidson

Master's Theses

Freshwater ecosystems are complex ecosystems driven by hydrologic, geomorphic, and biological processes. Considering both abiotic and biotic factors is important to understanding resident species habitat. Geomorphology is a known, but often neglected factor in habitat assessment for freshwater fishes. A crucial feature commonly seen in alluvial stream systems are sandbars, which are dynamic and variable sediment structures that are known to have many associated species. One such species is the Pearl Darter, Percina aurora, a federally threatened species that only resides in the Pascagoula River watershed. Despite its status, habitat preference remains unclear. To fill this knowledge gap, I …

The Impact Of Wild Pigs (Sus Scrofa) On Avian Occupancy And Vegetation Structure, Kenneth Wilson

Graduate Theses and Dissertations

Invasive species, particularly wild pigs (Sus scrofa), pose a significant threat to native ecosystems through both direct and indirect impacts. Wild pigs are large-bodied omnivorous species that can consume a wide variety of plants and animals and are known for destructive foraging behaviors, which likely threatens avian communities through habitat alteration and can impact the long term structure of ecosystems where they are present.

In chapter 1, we used a combination of avian point count surveys, vegetation assessments, and camera trap surveillance to quantify how the occupancy of breeding birds in bottomland hardwood forests changed in relation to …

Unravelling Large-Scale Patterns And Drivers Of Biodiversity In Dry Rivers, Arnaud Foulquier, Thibault Datry, Roland Corti, Daniel Von Schiller, Klement Tockner, Rachel Stubbington, Mark O. Gessner, Frédéric Boyer, Marc Ohlmann, Wilfried Thuiller, Delphine Rioux, Christian Miquel, Ricardo Albariño, Daniel C. Allen, Florian Altermatt, Maria Isabel Arce, Shai Arnon, Damien Banas, Andy Banegas-Medina, Erin Beller, Melanie L. Blanchette, Joanna Blessing, Iola Gonçalves Boëchat, Kate Boersma, Michael Bogan, Núria Bonada

Research outputs 2022 to 2026

More than half of the world’s rivers dry up periodically, but our understanding of the biological communities in dry riverbeds remains limited. Specifically, the roles of dispersal, environmental filtering and biotic interactions in driving biodiversity in dry rivers are poorly understood. Here, we conduct a large-scale coordinated survey of patterns and drivers of biodiversity in dry riverbeds. We focus on eight major taxa, including microorganisms, invertebrates and plants: Algae, Archaea, Bacteria, Fungi, Protozoa, Arthropods, Nematodes and Streptophyta. We use environmental DNA metabarcoding to assess biodiversity in dry sediments collected over a 1-year period from 84 non-perennial rivers across 19 countries …

In Vitro Investigation Of The Potential Therapeutic Implications Of Safranal On Gastric Cancer, Aisha Juma Al Otaibi

Theses

Despite the fact that gastric cancer patients are indispensable to conventional radiation and chemotherapy, the detrimental side effects of those approaches prompt cancer research to seek new integrative treatments. In this respect, safranal (SAF) stands out as a complementary phytotherapeutic drug with exceptional anticancer properties. Concurring studies have shown that SAF is negatively associated with several cancer types, yet its potential therapeutic impact on gastric cancer remains vastly obscure. Thus, this dissertation sought to investigate SAF's prospective tumoricidal ability and its underlying mechanisms of action on gastric cancer in vitro. Herein, the study evaluated the cytotoxicity of SAF on …

Accumbal Plasticity And Associated Behavioral Adaptations In The Spared Nerve Injury Model Of Chronic Neuropathic Pain, Yu-Hsuan Chang

Arts & Sciences Graduate Student Theses and Dissertations

Chronic pain is a debilitating neurological disorder that affects hundreds of millions of individuals worldwide. It is associated with cognitive symptoms such as cognitive inflexibility, which is defined as the inability to adaptively update behavior in response to changing environmental conditions. To date, there are no effective treatments for these symptoms, even though they substantially contribute to the decreased quality of life of chronic pain patients and are associated with reduced efficacy of pain management strategies. Therefore, understanding the neural circuit adaptations that drive cognitive symptoms of chronic pain, like cognitive inflexibility, is essential for improving existing treatments for this …

Red Snapper (Lutjanus Campechanus) Neurocranium, Michelle Bassali, Anthony D'Auria, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Variation In Diet And Leafy Browse Offerings Amongcolobus Monkeys (Colobus Spp.) And Langurs(Trachypithecus Spp.) In Aza Facilities, Maura R. Plocek, Noah T. Dunham

Student Scholarship

Providing nutritionally balanced diets is a key aspect of successful husbandry and can help support sustainable ex situ populations. This study focuses on the diets of zoo‐housed colobus monkeys (Colobus angolensis and C. guereza) and langurs (Trachypithecus cristatus and T. francoisi) across AZA institutions. We utilized survey responses from 34 institutions to (1)document the extent of dietary variation with regard to food categories and macronutrients offered, (2) assess interspecific variation in diets offered, and (3) identify important aspects of browse provisioning among institutions. A total of 95 different food items and 229 common‐name browse types were …

An Integrative Approach Clarifies Species Delimitation And Biogeographic History Of Debregeasia (Urticaceae), Amos Kipkoech, Ke Li, Richard I. Milne, Oyetola Olesegun Oyebanji, Moses C. Wambulwa, Xiao-Gang Fu, Dennis A. Wakhungu, Zheng-Yuan Wu, Jie Liu

Biological Sciences Faculty Publications and Presentations

Integrative data from plastid and nuclear loci are increasingly utilized to resolve species boundaries and phylogenetic relationships within major angiosperm clades. Debregeasia (Urticaceae), an economically important genus, presents challenges in species delimitation due to its overlapping morphological traits and unstable taxonomic assignments. Here, we analyzed 14 morphological traits and generated 12 data matrices from the plastomes and nrDNA using genome skimming from the nine recognized morphospecies to clarify species boundaries and assess barcode performance in Debregeasia. We also used a universal set of 353 nuclear genes to explore reticulate evolution and biogeographic history of Debregeasia. Plastomes of Debregeasia …

Species Distribution Modeling With Environmental Time Series Data And Deep Learning, Austin M. Smith

USF Tampa Graduate Theses and Dissertations

Species distribution models (SDM) are frequently used to gain ecological understanding and guide conservation decisions. These models are developed with a wide variety of procedures - from regression-based approaches to more modern machine learning algorithms - but a requirement almost all share is the use of predictor variables that strongly simplify the temporal variability of driving factors. These static features are chosen by the user, which can incorporate unnecessary bias into a study. Additionally, current data preparation techniques often remove variables from the process entirely, which inherently removes information that may still be of importance. Conversely, novel architectures of deep …

Assessing Fish Species Richness Using Edna And Seine Nets In A Restored Estuary In Tampa Bay, Florida, Us, Alex Seigel

USF Tampa Graduate Theses and Dissertations

Coastal estuarine systems associated with the Tampa Bay region along the Gulf of Mexico, Florida, US, are critical to the region’s fishing industry, which contributes significantly to Florida’s economy. Restoring estuaries along the Gulf Coast of Florida is an investment in Florida’s environmental health and economic stability. Estuaries serve as nurseries for many marine species, including commercially important organisms like finfish. Restoring these areas can enhance fish stocks and, by extension, boost the economic returns from commercial and recreational fishing. In this study, differences in fish species richness were compared among a seven-month period in 2022, seine nets (once in …

Ecological Epidemiology Of The Fungal Pathogen Ophidiomyces Ophiodiicola In Southeastern U.S. Snake Populations: Distribution, Drivers, And Anthropogenic Risk Factors, Shivam Shukla

USF Tampa Graduate Theses and Dissertations

Studying wildlife diseases is an important conservation measure, as many pathogens are a critical threat to biodiversity worldwide. Ophidiomycosis is an emerging infectious disease caused by the invasive fungal pathogen Ophidiomyces ophiodiicola that affects a wide variety of snake species, often causing mortality. There has been limited surveillance for ophidiomycosis in the southeastern United States’ (US) snake populations and research into the effects of anthropogenic disturbance on disease spread is also lacking. Thus, this project aimed to quantify the distribution and drivers (taxonomic, anthropogenic, and morphological) of ophidiomycosis in Florida, Louisiana, and Mississippi’s free ranging snakes as well as study …

Study On The Seasonal Endobacterial Profile Of Avicennia Officinalis, Focusing On Core Bacterial Consortia Within Mangrove Pneumatophores, Devi R R, Vinod Prabhakar

Karbala International Journal of Modern Science

Microhabitats are smaller pockets of ecosystems with unique conditions and they serve as major contributors of species richness. Mangrove plants offer a diverse array of such microhabitats (rhizosphere, phyllosphere, endosphereetc), and the inhabitant bacteria benefit the host by providing better salinity tolerance, faster and better nutrient mobilization, protection from phytopathogens and assistance in seed germination. Identification of such beneficial bacteria,their growth requirementsandmetabolism, willhave direct application in the field of agriculture and ecosystem restoration. Hence in this study, effort has been made to elucidate the seasonal numerical and compositional profile of pneumatophore bacterial consortia of Avicennia officinalis, from the mangrove …