Digital Commons Network^™

Salinity Effects On Cu(Ii) Bioremediation By Chlamydomonas Reinhardtii Microalgae, Jennifer Hughes

Honors Undergraduate Theses

Microalgae offer a sustainable approach to wastewater treatment by removing pollutants such as excess nutrients, CO₂, and heavy metals. Among various species, Chlamydomonas reinhardtii shows strong potential in removing Cu(II) ions from wastewater. In addition, salinity stress in C. reinhardtii has been linked to morphological and metabolic changes that enhance lipid and phytochemical production. While salinity stress has been studied for enhancing biofuel production, its impact on microalgal removal of heavy metals, particularly Cu(II), remains largely unexplored. Understanding how varying salinity conditions affect both algal growth and the efficiency of Cu(II) remediation could provide critical insights for improving integrated wastewater …

Parasite Communities Of Bluntnose Minnow (Pimephales Notatus) From Several Creeks In East Central Illinois, Md Rowshan Alam

Masters Theses

Parasites of freshwater fish play a vital functional role in aquatic ecosystems, but the spatial and ecological determinants of their prevalence and characteristics are still poorly understood. The current study quantified the diversity, prevalence, and environmental determinants of whole parasite burdens in bluntnose minnows across multiple creeks in central Illinois. Fish sampling and the collection of stream habitat data were conducted during the summers of 2023 and 2024. Fish were examined for parasites using necropsy and histology. A total of 20,817 parasites, predominantly trematodes (Posthodiplostomum spp.), cestodes (Proteocephalus spp), and protozoans, were identified. The most prevalent and abundant …

Assessing The Role Of Daily Thermal Fluctuations In The Performance Of Mitochondrial Energy Transduction Of The Centrarchid, Lepomis Cyanellus, Grayson Hofmann

Masters Theses

Freshwater systems are affected by current climate extremes, forcing species found in these systems to withstand drastic changes in temperature. Temperature changes affect these organisms by altering their metabolic rate and energy balance. Increases in temperature can lead to an increase in oxygen consumption, which ultimately increases the need for more energy production. Mitochondrial energy transduction of green sunfish (Lepomis cyanellus) and many other fish species after a thermal insult, has hardly been explored. This study complements the limited research available on mitochondrial function in similar species, as they ignore the possibility of consecutive thermal events, which are …

Chromatin Remodeling Protein Bptf Mediates Chromatin Accessibility At Gene Promoters In Planarian Stem Cells, Prince Verma, John M. Allen, Alejandro Sánchez Alvarado, Elizabeth M. Duncan

Biology Faculty Publications

Background The regulation of chromatin accessibility is essential in eukaryotic cells as one of several mechanisms that ensure gene activation occurs at appropriate times and in appropriate cell types. Accordingly, mutations in chromatin remodeling proteins are linked to many different developmental disorders and cancers. One example of a chromatin protein that has been linked to both developmental abnormalities and cancer is BPTF/NURF301, the largest subunit of the Nucleosome Remodeling Factor (NuRF) complex. The BPTF subunit is not only important for the formation of NuRF but also helps direct its activity to particular regions of chromatin by preferentially binding histone H3 …

Spatial Analysis Of American Chestnut (Castanea Dentata) Distribution And Ecology In Kentucky’S Federal Lands, Jacob Raymond Pease

Murray State Theses and Dissertations

Anthropogenic factors have been the cause of the decline of tree species across the globe. From unsustainable logging and development to disease and improper management, threats to forests have taken their toll. The American chestnut (Castanea dentata) was once an integral basal tree species in Appalachian forests, but chestnut blight drove the species to functional extinction by the mid-1900s. The recent creation of genetically modified chestnuts gives hope for future restoration of the species throughout its historical range. Despite blight resistance, effective restoration will require optimal site selection for finding and planting chestnuts, which will maximize limited resources. …

Florida Statewide Coastal Restoration Guide, Melinda J. Donnelly, Matthew Martin, Christen Fleming, Kelly Kibler, Linda Walters, Patrick Shearer, Lauren Jennison, Erika Lin, George Zaragoza

Faculty Scholarship and Creative Works

The Statewide Coastal Restoration Guide (SCRG) is a comprehensive planning resource developed through a collaboration between Florida Department of Environmental Protection (FDEP), University of Central Florida (UCF), and Resource Environmental Solutions (RES) to strengthen and unify shoreline restoration efforts across Florida. With over 4,500 miles of estuarine shoreline, Florida’s coasts are vital to the state’s ecological health, economic prosperity, and community resilience. However, they face increasing threats from sea level rise, stronger storms, erosion, habitat degradation, and development. The SCRG responds to these challenges by providing science-based, non-regulatory guidance for implementing resilient, nature-based restoration strategies that protect both ecosystems and …

Elements Of Social Capital And Counterspace Processes Contribute To Undergraduate Stem Student Development Of A Sense Of Belonging, Mackenzie J. Gray, Jasmine N. Yacobian, Erin Shortlidge

Biology Faculty Publications and Presentations

Having a sense of belonging can promote persistence in the STEM fields, but less is known about what it means to develop that sense of belonging. To investigate this phenomenon, we conducted semi‐structured interviews with a cohort of STEM students (n = 10) nearing graduation at an urban university regarding their sense of belonging and qualitatively coded the interviews using thematic analysis. Results revealed that all interviewed students clearly articulated feelings of belonging, making them an ideal population from which to learn more. We applied two frameworks to guide our understanding of what factors promoted the development of a sense …

Adolescent Seizure Impacts Oligodendrocyte Maturation, Neuronal-Glial Circuit Formation, And Myelination In The Mammalian Forebrain, Kylie Foutch, Iris Tilton, Aundrea Cooney, Cole Bender, Collin Licharz, Megan Baldemor, Caitlyn Rock, Atehsa Asal Sahagun, Robert Brock, Chloe Franzia, Mary Francis Garcia, Raghav Gupta, Christopher Arellano Reyes, Mariyam Lokhandwala, Daniela Moura, Hirofumi Noguchi, Laura Cocas

Biology

Oligodendrocyte progenitor cells differentiate into oligodendrocytes, which myelinate axons during development and following demyelinating injury. However, the mechanisms that drive the timing and specificity of developmental myelination are not well understood. We hypothesized that oligodendrocyte progenitor cell proliferation and differentiation would be affected by pathological neuronal activity during adolescent development when developmental myelination is occurring and that this would also impact neuron-to-oligodendrocyte progenitor cell connectivity and myelination. We used kainic acid to induce a seizure in mice, treating equal numbers of males and females, in sample sizes of at least five animals. We found that the seizures led to increased …

Dataset For "The Role Of Metacognition And Confidence In Quantitative Reasoning In College Biology Courses", Joseph Dauer, Anum Khushal, Brian A. Couch, Robert L. Mayes, Kent Rittschof

School of Natural Resources: Faculty Publications

Quantitative modelling is a fundamental skill for biology students with performance determined by a host of cognitive and metacognitive resources and requiring instructors to integrate key quantitative dimensions into teaching biology. Using an assessment of quantitative modelling in biology, we explored biology students’ (n = 930 higher education students) modelling practices (i.e., abilities to create, test, revise), metacognition about the nature of models and confidence in their capabilities to model. Students performed best on items related to understanding variables used in models and performed more poorly on items related to interpreting, creating, and using models to understand biological phenomena. …

Racial Health Disparities Among Pregnant Women Of Color In New York City: Biological And Systemic Impacts, Kaylin Peña

Pitzer Senior Theses

Racial Health Disparities Among Pregnant Women of Color in New York City: Biological and Systemic Impacts

Abstract

This thesis aims to explore how racial health disparities, underrepresentation in gynecology, and socioeconomic barriers contribute to adverse pregnancy outcomes among women of color in New York City. Through an interdisciplinary approach that combines resources from medical anthropology and human biology, this research will examine the biological implications of chronic stress, specifically HPA axis dysregulation, and its role in maternal health outcomes. With the idea of intersectionality, as conceptualized by Kimberlé Crenshaw, the study analyzes the various effects of systemic inequities and social …

Identification Of Host-Pathogen Protein Interactions: Yeast Two-Hybrid Analysis Of Sars-Cov-2 Nsp10 And Human Cellular Proteins, Antonios Argyriou

Selected Full-Text Master Theses 2021-

The ongoing COVID-19 pandemic, caused by SARS-CoV-2, has resulted in over 774 million cases and 7 million deaths worldwide, establishing it as one of the most devastating pandemics in recorded history. While current therapeutic approaches primarily target viral entry mechanisms and specific enzymes, the limited understanding of non-structural protein functions hinders the development of comprehensive antiviral strategies. This research focuses on Nsp10 (non-structural protein 10), a highly conserved 139-amino acid protein essential for viral mRNA capping and replication. Nsp10 serves as a critical cofactor in the formation of methyltransferase complexes with Nsp14 and Nsp16, playing a vital role in viral …

Coordinating The Energetic Strategy Of Glia And Neurons For Memory, Hannah Shoenhard, Amita Sehgal

Biology Faculty Research and Scholarship

Memory consolidation requires rapid energy supply to neurons. In a recent study, Francés et al revealed the signal by which a neuron commands glia to limit fatty acid synthesis in favor of metabolite export during memory formation in Drosophila melanogaster. This mechanism coordinates just-in-time glial energy delivery in response to dynamic neuronal needs.

Periconceptual Nutrition And The Preimplantation Embryo: Approaches For Intervention And Generational Health, Abigail Klein

Dissertations and Theses

The typical American diet exceeds dietary fat recommendations, contributing to the rising rates of obesity. This negatively impacts fertility, pregnancy and fetal growth, and lifelong offspring health. During pregnancy, this also increases risk of co-morbidities like gestational diabetes. Despite this, lipids are not screened during pregnancy and limited research exists evaluating these co-exposures on offspring health. Our lab used a rat model of maternal high fat (HF) diet with late-gestation diabetes (DM) to demonstrate that mitochondrial dysfunction and oxidative stress incite lifelong cardiometabolic disease risk in offspring. To determine the timing of damage, we hypothesized that maternal overnutrition incites mitochondrial …

If You Give A Tadpole A Chemosensory Stimulus: Detection Of Invasive Predatory Cues And Signs Of Conspecific Communication In Pseudacris Sierra Tadpoles, Faben Tesfazion

Scripps Senior Theses

Amphibians are at disproportionately higher risks of extinction than all other vertebrate classes, and a major causing factor is invasive predation. The American bullfrog (Lithobates catesbeianus), native to Eastern North America, is a notorious invasive species which preys on native Sierran treefrogs (Pseudacris sierra) in Northern and Central California. These treefrogs have yet to be placed on any Special Animals List, suggesting the presence of adopted behaviors against predation. Past studies have documented the importance of chemical sensing in anuran tadpoles as predator detection tools. This study tests if Sierran treefrog tadpoles can recognize invasive chemical …

Promoting Acorn Survival Using Capsaicin Seed Coatings Is Strengthened By The Removal Of Invasive Shrubs, Mark Fuka, Brian M. Connolly, John L. Orrock

Biology Faculty Scholarship

Promoting regeneration of native trees, like Quercus spp., is a priority for land managers given the ecological and economic importance of oak woodlands. Although direct seeding may promote recruitment of Quercus spp., the effectiveness of direct seeding may be greatly reduced in environments where the activity of granivorous rodents is high. Importantly, the activity of granivorous rodents may be highest in environments where oak restoration is most desired, such as habitats invaded by non-native woody shrubs. Implementing chemical deterrents to granivory should promote direct seeding success; yet it is essential to understand if those deterrents are effective in challenging restoration …

Physiological Responses And Plant Trait Interactions With Coastal Dune And Swale Development, Emily C. Riffe

Theses and Dissertations

Vegetation is crucial to coastal ecosystems experiencing climate change and storm-induced disturbance. Plant species interact with the physical environment and are considered “ecosystem engineers.” Sand dunes and barrier islands are at the forefront of climate change impacts as they are barriers that protect landward habitat and communities. Nature based solutions are utilized to protect infrastructure on developed coastlines. Understanding how dominant plant species interact with and alter the physical environment enhances our understanding of the mechanisms of change and consequences to the system. The goal of this research was to quantify physiological and functional trait differences in dominant species found …

Quantifying The Vulnerability Of Smooth Dogfish And Winter Skate To Electromagnetic Fields From Offshore Wind Transmission Cables In The Mid-Atlantic Shelf, Rachel A. Sechrist

Theses, Dissertations and Culminating Projects

The development of offshore wind structures in the northeastern U.S. will contribute to renewable energy goals, but will overlap with many marine species as well as economically important fisheries. High voltage transmission cables from offshore wind farms emit electromagnetic fields (EMFs) that elasmobranchs may detect using sensory organs known as ampullae of Lorenzini. However, sensitivity to EMFs varies by species due to differences in the number and arrangement of pores, the length of subdermal canals within the ampullae of Lorenzini sensory network, and the habitats they reside and regions they forage. For species who reside or forage in benthic regions, …

Neuroimmune Toll-Like Receptor Signaling Modulates C. Elegans Feeding Behavior In A Hunger State-Dependent Manner, Retwika Ganguly

Theses, Dissertations and Culminating Projects

Animals face the risk of encountering pathogenic microbes while searching for nutritive resources. Assessing the interplay between the benefits of nutrition vs. the risk of infection might influence the behavioral regulation of immune processes. In Caenorhabditis elegans (C. elegans), a nematode roundworm, behavioral immunity is partially regulated by the innate immune molecule TOL-1: a homolog of vertebrate Toll-like Receptor (TLR) proteins. TOL-1 influences C. elegans pathogen avoidance behaviors by promoting the development of CO2-detecting chemosensory neurons, which detect a signal linked to the presence of harmful pathogens. While TOL-1′s role in pathogen avoidance is well established, its impact in an …

Gene Expression And Dna Methylation Changes In Response To Hypoxia In Toxicant-Adapted Atlantic Killifish (Fundulus Heteroclitus), Neelakanteswar Aluru, Yaamini R. Venkataraman, Christopher S. Murray, Veronica Depascuale

Biology

Coastal fish populations are threatened by multiple anthropogenic impacts, including the accumulation of industrial contaminants and the increasing frequency of hypoxia. Some populations of the Atlantic killifish (Fundulus heteroclitus), like those in New Bedford Harbor (NBH), Massachusetts, USA, have evolved a resistance to dioxin-like polychlorinated biphenyls (PCBs) that may influence their ability to cope with secondary stressors. To address this question, we compared hepatic gene expression and DNA methylation patterns in response to mild or severe hypoxia in killifish from NBH and Scorton Creek (SC), a reference population from a relatively pristine environment. We hypothesized that NBH fish would show …

Are Blow Flies (Diptera: Calliphoridae) Active Nocturnally In South Georgia?, Breana R. Manning

Honors College Theses

Medicolegal forensic entomology uses insect colonization of remains to estimate minimum post-mortem intervals (mPMI). Blow flies (Diptera: Calliphoridae) are among the earliest colonizers, yet their nocturnal activity remains poorly understood. This study aimed to determine whether blow flies are active at night and whether artificial light influences their behavior. Using baited bottle traps in both artificially lit and unlit environments near Georgia Southern University, flies were collected over two trials in fall 2024. Of the 1,843 blow flies collected, only six were captured at night (0.003%), suggesting minimal nocturnal activity. Lucilia coeruleiviridis preferred unlit environments. In contrast, Cochliomyia macellaria was …

Rivers To The Deep Sea – Thoughts On My Career And Profession, Steve W. Ross

Gulf and Caribbean Research

Abstract: In this essay I review my 45+ year career and some of the significant changes I have observed. This journey involved research in rivers and streams, estuaries, coastal systems (including the continental shelf), and the deep sea to a depth of about 4,000 m. After completing undergraduate (BS, Duke University) and graduate (MA, University of North Carolina – Chapel Hill and PhD, NC State University) degrees in zoology, I found jobs as a Fishery Biologist (NC Division of Marine Fisheries), a program Research Coordinator (NC National Estuarine Research Reserve), and a Research Professor (University of NC – Wilmington). Between …

Brachial Plexus Lesions: Uncommon Causes And Clinical Sequelae, Lucy Greenhagen

Biology and Microbiology Graduate Students Plan B Research Projects

The brachial plexus is a complex network of nerves that transmit motor and sensory information between the spinal cord and upper extremities. Brachial plexus lesions may be caused by a broad range of congential and traumatic conditions. These lesions present a challenge to healthcare providers both for diagnosis and treatment. While variations in the brachial plexus have been widely reported, resources outlining the prevenance and involvement of anatomical variation along with their possible clinical presentation are more limited. The objective of this review is to describe uncommon causes of brachial plexus lesions and the clinical challenges they present for providers. …

Alternative Treatments And Potential Cures For Type 1 Diabetes, Dillon Peterson

Biology and Microbiology Graduate Students Plan B Research Projects

Type 1 diabetes (T1D) is an autoimmune disease that affects multitudes of people without a cost-effective cure. T1D is caused by the immune system reaching a threshold where it then begins to attack the beta cells in the pancreas causing inflammation, which eventually leads to the death of beta cells. Once a large majority of the beta cells die off, the pancreas begins to inadequately supply insulin to the body, which is when the symptoms of T1D begin to emerge. To treat these symptoms, the person must utilize synthetic insulin. The utilization of synthetic insulin, along with the plethora of …

Epilepsy: Etiology And Treatment Methods, Avital Nightingale

The Science Journal of the Lander College of Arts and Sciences

Epilepsy is a common neurological disorder that is caused by abnormal electrical activity in the brain. Due to the evolution of our understanding of epilepsy and its causes, a variety of therapeutic options have been developed to modulate seizures and limit their occurrence rate. Medication, diet and surgery have been the traditional treatments offered to individuals suffering from epilepsy but the latest advancements in neurotechnology have given patients with drug resistant epilepsy hope by providing options other than surgery. While the disorder is a complex one and its pathogenesis not yet fully understood, recent research has provided insight into the …

Neonatal Immune System Development, Miriam Solomson

The Science Journal of the Lander College of Arts and Sciences

The immune system is essential for the body’s function and survival, consisting of two main components, the innate and adaptive immune systems. This paper will explore the various components of the neonatal immune system, such as the innate and adaptive or acquired systems, the initial colonization of microbes, and the crucial role of maternal antibodies. Additionally, this paper will delve into the significance of breastfeeding, the influence of different birth methods on gut microbiota, and the success of vaccines on neonates. This research paper’s goal is to provide insight into neonatal immune system development, the various factors that have an …

Mind Over Matter: Do Nootropics Hold The Key To Fighting Alzheimer’S?, Ben Berkowitz

The Science Journal of the Lander College of Arts and Sciences

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, memory loss, and impaired daily functioning. Nootropics, a class of cognitive-enhancing compounds, have recently emerged as potential therapeutic agents for mitigating the cognitive impairments associated with AD. This paper reviews the current evidence regarding the efficacy (or lack thereof) of different treatments used in the past for treating Alzheimer’s, including cholinesterase inhibitors and anti-Aβ antibodies. The findings are compared to those using various nootropics, including natural supplements and synthetic compounds, in modulating neurobiological pathways relevant to AD pathology. Mechanisms of action such as neuroprotection, antioxidant activity, acetylcholine modulation, …

Strategies To Address The Growing Impact Of The Antibiotic Resistance Crisis, Rina Balter

The Science Journal of the Lander College of Arts and Sciences

As time progresses, more bacterial strains are becoming resistant to the antibiotics developed to treat them. Impeding such intractable infections should be a major clinical priority, as previously treatable diseases have begun to incur a fatality rate that will escalate catastrophically unless it is halted. This research paper explores relevant information regarding the advances in developing alternative strategies to antibiotics to combat antimicrobial resistant (AMR) bacteria, within the context of clinical application. Recognizing the profound impact antimicrobial resistance (AMR) will have on patient health enables the implementation of proactive measures to curb the expanding prevalence of multidrug-resistant (MDR) pathogens. This …

Retinal Vision Restoration, Aharon S. Galandauer

The Science Journal of the Lander College of Arts and Sciences

Retinal degeneration is a leading cause of vision loss worldwide due to the retina’s inability to regenerate specialized cells after injury or stress. This study examines the potential for retinal regeneration by assessing current research on Müller glial cell differentiation and stem cell-based treatments. An analysis of experimental studies and therapeutic advancements highlights the mechanisms observed in regenerative models such as zebrafish and chicks, in contrast to the reactive gliosis and glial scarring observed in humans. Findings suggest that therapies such as optogenetics and stem cell transplantation have partially restored vision; however, their limitations stem from an inability to fully …

Is Placenta Stem Cell Banking Worthwhile?, Chaya Nathan

The Science Journal of the Lander College of Arts and Sciences

Regenerative medicine has gained significant traction due to its potential to repair or replace damaged tissues and organs, with stem cell therapies emerging as a promising avenue for treating various diseases. While bone marrow-derived MSCs (BM-MSCs) are commonly used in clinical settings, their limitations, such as the invasive extraction procedure and limited proliferation capacity, underscore the need for alternative stem cell sources. Placenta-derived mesenchymal stem cells (PDMSCs) represent a promising solution, offering advantages such as greater proliferative capacity, faster expansion rates, and reduced risk of immune rejection compared to adult stem cells. The placenta is a rich source of MSCs, …

Can Organs Be Generated From A Patient’S Own Stem Cells?, David Heimowitz

The Science Journal of the Lander College of Arts and Sciences

The global shortage of transplantable organs represents a critical medical challenge, with current organ donation systems meeting less than 10% of the worldwide need. This situation has spurred intensive research into regenerative medicine, particularly focusing on the potential of generating organs from a patient’s own stem cells. This review examines the current state of stem cell research and its applications in organ generation, analyzing both the progress made and the challenges that remain. Through a comprehensive analysis of recent literature and clinical trials, the study explores the fundamental principles of stem cell biology, current therapeutic applications, and the role of …