Digital Commons Network^™

Cell Cycle Inhibition: Illudin S And Its Effects On Cdk2 In Ewing Sarcoma, Stephanie Diez, Natalie Flores, Alexandria Hernandez, Viviana Palacios, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcomas are aggressive round cell mesenchymal neoplasmas that have a high occurrence in children and young adults. CDK2 is a protein that is involved in the G1 phase of the cell cycle, which promotes the transition to the S phase. CDK2 kinase activation is mainly observed in the G1/S-phase transition.3 CDK2 binds to Cyclin proteins is responsible for entry and progression, thereby leading to maximal apoptosis activity in the S-phase. Illudin S is a drug that has been known to target cancer-specific cells, such as leukemia. Illudin S, in general are a cytotoxic metabolite that comes from plants, specifically …

Yap1 Knockdown And Panobinostat Treatment Increase Apoptotic Response And Decrease Gene Expression In Ewing Sarcoma Cells, Luna Collazo-Garcia, Alejandra Favela Santos, Madeline Torres-Salazar, Isabella Toscano, Terry Jo Shackleford

Cell and Molecular Methods

Ewing sarcoma (EWS) is considered to be one of the most aggressive pediatric malignancies, often characterized by its dysregulated gene expression driven by oncogenic fusion proteins. The Hippo signaling effector Yes-associated protein 1 (YAP1) has been known in promoting cell proliferation, survival, and therapeutic resistance in multiple cancers. However, its role in Ewing sarcoma response to treatment remains unclear. This study investigated whether YAP1 knockdown enhances the sensitivity of Ewing sarcoma cells to the histone deacetylase inhibitor Panobinostat. Ewing sarcoma, ES8, cells were transfected with a YAP1-targeting siRNA (siYAP1) or a non-targeting control (siControl) and treated with DMSO, 0.1 μM, …

Everolimus Treatment Combined With Akt1 Knockdown Increased Apoptosis And Decreased Cell Proliferation In Ewing Sarcoma Cells, Arisha Arif, Alfie Barcenez, Sophia Ruter, Nicole Vanegas-Riddick, Terry Jo Shackleford

Cell and Molecular Methods

The purpose of this study is to use the gene AKT1, due to the interest in AKT1’s role in cancer cell proliferation, and the drug Everolimus, to determine if combined targeted therapy works as a more efficient therapeutic approach. Ewing Sarcoma has been connected to chromosomal translocations and is most common in pediatric patients. It is most often treated with chemotherapy and local treatments. It may be connected to the gene AKT1 due to how it regulates cell metabolism, growth, and proliferation. The gene mTOR is similarly connected to cell metabolism and proliferation, and is inhibited by the drug Everolimus. …

Everolimus And Sirna-Mediated Mtor Inhibition Reduces Proliferation And Promotes Apoptosis In Ewing Sarcoma Cells, Dylan Calvert, Malorie Martinez, Alexa Miner, Ellis Quiroga, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcoma (ES) is an aggressive pediatric bone cancer characterized by rapid cell proliferation and poor prognosis, making the identification of therapeutic targets critical. One of  the mechanistic targets is rapamycin (mTOR) signaling pathway, which is critical for regulating cell growth, proliferation, and survival. Abnormal activation of the mTOR signaling pathway has been linked to the progression of ES, as it drives uncontrolled cell growth and apoptosis resistance. Because mTOR represents a promising therapeutic target for ES treatment, we hypothesized that inhibiting mTOR activity through an siRNA-mediated knockdown or through Everolimus drug treatment, would reduce cell proliferation and promote ES …

Sirna Knockdown Of Rptor Reduces Rptor Expression, Increases Brd4 Expression, And Alters Proliferation And Apoptosis In Ewing's Sarcoma Cells, Sergio Cipriano, David Leavitt, Michael Olivia, Liam Valdez, Terry Jo Shackleford

Cell and Molecular Methods

Ewing sarcoma is a highly aggressive cancer that primarily affects children and young adults. Although treatment options exist, many patients do not respond effectively, showing the need for improved targeted therapies. RPTOR is a key in mTORC1 complex which regulates cell growth, proliferation, and survival. LY2874455 is a selective pan-FGFR inhibitor that targets growth factor signaling pathways involved in tumor progression, and FGFR signaling interacts with pathways such as mTOR, making it a potential target for combination therapies. We hypothesized that silencing RPTOR in Ewing sarcoma cells would disrupt mTOR signaling and alter expression of genes linked to cell survival …

The Influence Of Prosthetic Obturators On Quality Of Life In Individuals With Maxillectomy Defects, Minseok Kim

Oral Health

Abstract

Introduction: In recent years, there has been a growing focus on understanding the impact of oral cancer on patients' quality of life. Prosthetic rehabilitation techniques, such as maxillary obturators, often in conjunction with reconstructive surgery, have emerged as interventions to address the challenges posed by post-maxillectomy abnormalities.

Objective: This study aims to systematically review and evaluate the effectiveness of maxillofacial prosthetic rehabilitation using obturators in enhancing the quality of life of patients following maxillectomy.

Methodology: Systematic review of identified literature spanning the timeframe from 2000 to 2020 was used. The comprehensive search encompassed widely recognized databases including PubMed, …

Identifying And Coping With Failure In Human Anatomy & Physiology I And Ii Students, Annie R. Mueller

Honors Theses

Human Anatomy and Physiology (A&P) is a two-semester course that focuses on the structure and function of the human body systems. For many students, A&P could be their first challenging undergraduate course and their first experience with academic difficulty. Students must learn to adapt their study habits and respond productively to academic failure. The purpose of this study was to examine how students in the A&P courses at the University of Mississippi perceive and cope with academic failure, as well as to evaluate the effectiveness of an intervention method. Students enrolled in BISC 206 and BISC 207 participated in three …

Bio 340 Final Project: Effects Of Zoning On Fish Density Along The Great Barrier Reef, Mary Lamoriello

Biology Student Scholarship

Mary Lamoriello - Environmental Biology '27

Over the years, there have been many marine parks and restricted fishing zones established along the Great Barrier Reef to maintain biodiversity and coral health. The goal of these zones is to create an ”undisturbed” area, where species can flourish. Many different species reside in this reef and play important ecological roles. Parrotfish help maintain coral health by grazing on algae, and the presence of carnivores often indicate a diverse and healthy ecosystem. This project aims to examine the differences in species distribution between zones along the Magnetic Island, by looking at carnivorous and …

Bio 340 Final Project: Rural Population Structure And Food Production Interact To Shape Global Undernourishment, Ava Ripanti

Biology Student Scholarship

Ava Ripanti - Environmental Biology '27

Undernourishment remains a major global public health challenge, reflecting the proportion of a population lacking sufficient dietary energy intake. While food availability is central to food security, undernourishment is also shaped by structural and socioeconomic factors such as population distribution and access to resources. Although rural populations are closely tied to agriculture, they often experience higher food insecurity due to limited infrastructure, market access, and healthcare. Prior research emphasizes that broader determinants, particularly inequality and development, are stronger predictors of undernourishment than agricultural output alone, that food insecurity exists in both rural and urban …

Bio 340 Final Project: Effects Of Light Pollution On Bat Activity, Cael Schneider

Biology Student Scholarship

Cael Schneider - Environmental Biology ‘28

Artificial light at night (light pollution) is an increasing consequence of urbanization and can disrupt nocturnal wildlife. Bats are especially susceptible, as they rely on dark skies for navigation, predator avoidance, and foraging. The effects of artificial light may vary among species due to differences in foraging strategies, habitat use, and light tolerance.  Previous research has documented negative effects of artificial light on bat foraging, roosting, breeding, and hibernation. However, some species, such as red bats, may forage near artificial lights where insects are abundant. Species including the big brown bat, hoary bat, and …

Bio 340 Final Project: A Comparative Analysis Of German And French Energy Policy, Adam Garies

Biology Student Scholarship

Adam Garies - Environmental Biology ‘28

European energy systems have undergone rapid transformation over the past two decades, with countries adopting different strategies to reduce carbon emissions. This divergence is particularly evident between Germany and France. Following the Fukushima disaster in 2011, Germany accelerated its nuclear phase-out, while France has declared its commitment to nuclear energy. Nuclear energy however, is an important step towards reducing carbon emissions. Excluding nuclear energy from energy plans will lead to a rise in reliance on potent fossil fuels like coal and natural gas, resulting in greater carbon emissions, even with an emphasis on expanding …

A Survey Of Lichen Native To Three Of Mississippi’S National Forests, William S. Granberry Iii

Honors Theses

Lichens are a major part of the environment for telling the air quality and the impact humans have on the environment. These signs go into how many species of lichen are present in a general area, and there has been a lack of a list of lichens that call Mississippi their home. The goal of this research is to help create this list through a survey of lichen in three of Mississippi’s National Forests: DeSoto, Homochitto, and Holly Springs. These three forests are ideal because they cover differing ecosystems and are in different parts of the state, heightening the chance …

Mechanisms Underlying Diversity In Bacterial Biofilms, Jacob David Holt

Dartmouth College Ph.D Dissertations

Bacteria commonly exist in natural environments as communities of multiple strains and species attached to surfaces and encased in an extracellular matrix of DNA, protein, and polysaccharides–termed biofilms. These biofilms contribute to diverse functions, from ocean carbon cycling via chitin degradation to pathogenic processes such as wound or implant colonization. The molecular mechanisms governing biofilm formation and composition have been well characterized in model pathogenic organisms, yet the extent to which aspects of biofilm formation contribute to the assembly and maintenance of diverse communities remains less well understood. Using microfluidics, confocal microscopy, and model biofilm-forming bacteria, this thesis details how …

Life Histories Of Two Sgcn Procambarus Species In East Texas, Mckenzie A. Adkins

Biology Theses

Within freshwater systems, crayfish play notable roles by shaping habitats and linking food web levels. Yet even with such influence, knowledge about numerous species is incomplete, which hinders protection strategies. Found only in the Neches River basin in eastern Texas, Procambarus nechesae and Procambarus nigrocinctus have different conservation statuses, mainly because data on their life history remains sparse. These two species are not closely related and are analyzed independently in this study. Research into traits tied to survival and environmental preferences was carried out so that informed decisions on conservation efforts can be made for these species. The goal of …

Bio 340 Final Project: Artificial Reef Effects On Fishing Productivity, Griffin Gouck

Biology Student Scholarship

Griffin Gouck - Environmental Biology '26

A slew of artificial reefs can be seen dotting the Gulf of America. Some result from catastrophic shipwrecks and others from construction dumping; however, all provide an opportunity for the development of marine ecosystems. The question of how artificial reefs effect local fish populations is an expansive concept extremely hard to simplify down to a basic relationship. The development of reefs is a delicate process taking place over many years of growth. Previous studies utilized a combination of resources like theoretical models, field data and comparisons to natural reefs to study how these artificial …

Impacts Of Salinity On Microbial Enzyme Activity In Coastal Waters, Natali Ariyoshi

Honors Theses

Microbial extracellular enzymes are critical for the breakdown of organic matter and nutrient cycling in aquatic ecosystems. Extracellular enzyme activity serves as an indicator of nutrient turnover rates, metabolic states, and nutrient limitations within an ecosystem. Coastal ecosystems are especially dynamic as the influx of freshwater from inland runoff or seawater intrusion from storm surges can lead to fluctuations in salinity which can restructure microbial communities and alter enzymatic activity. Despite the ecological significance of these changes, few studies have explored the effects of short-term salinity shifts on microbial extracellular enzyme activity in coastal waters. This study investigated the effects …

Studying More, Not Less: Rethinking Test Anxiety In Undergraduate Biology Courses, Kailey E. Reeves

Honors Theses

This study investigates the relationships among test anxiety, study behaviors, and metacognitive evaluation in undergraduate biology courses. While prior research has established that study strategies and metacognition influence academic performance, less is known about how these factors interact with test anxiety in undergraduate Biology courses. Guided by the student approaches to learning (SAL) and metacognition frameworks, this study examines whether test anxiety is associated with the type and number of study strategies students use, as well as their ability to evaluate their own learning and preparedness. Using a convergent parallel mixed-methods design, data was collected from 205 students enrolled in …

Ecological Drift And Host Filtering Jointly Structure Foliar Endophytes During Ecosystem Development., Caio César Pires De Paula, Petr Macek, Milan Varsadiya, Jakub Borovec, Mehmet A. Balkan, Brett S. Younginger, Daniel J. Ballhorn, Tomáš Picek, Tomáš Hájek, Jan Frouz, Jiří Bárta, Dagmara Sirová

Biology Faculty Publications and Presentations

Foliar endophytes contribute to plant nutrient acquisition, stress tolerance, and pathogen resistance, yet their responses to ecosystem-level processes remain poorly understood. Using a space-for-time substitution design, we investigated bacterial and fungal community dynamics in the foliar endosphere of four phylogenetically distinct plant hosts across a well-characterized successional chronosequence.

Proximity Data And Social Networks: A Validation Study Of Two Models Of Mobile Dataloggers In The Semi-Fossorial And Social Rodent, Octodon Degus, L. D. Hayes, A. A. Maldonado-Chaparro, Jessica Morales Valenzuela, L. Platt, L. A. Ebensperger

Biology Faculty Works

The use of mobile dataloggers to gather proximity data has generated novel insights into the social network structure of animals. The performance of mobile dataloggers may be impacted by different environmental and social conditions. Thus, validation studies that account for species-specific habitat use and behavior are essential. We conducted a series of validation studies of two brands of mobile dataloggers (Gipsy6, Technosmart, Italy; ProxLog, IoSA, Belgium) on the semi-fossorial rodent, Octodon degus. First, we determined the sensitivity of both types of dataloggers to track position and closeness in different habitats (Experiments 1 and 2). To determine if the structure …

The Ccu Disease Hunters: Putting Undergraduate Research To Work To Improve Community Health And Training The Next Generation Of Doctors, Paul E. Richardson

Journal of the South Carolina Academy of Science

No abstract provided.

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

Reptiles Of The Northeastern Piedmont Of South Carolina, Steven E. Fields

Journal of the South Carolina Academy of Science

No abstract provided.

Antibiotic Resistance Patterns In Bacterial Isolates From Mississippi Gulf Coastal Environments, Lawler F. Horn

Honors Theses

Coastal environments host diverse microbial communities that play essential ecological roles but may also serve as reservoirs for antibiotic-resistant bacteria. This study investigated patterns of antibiotic resistance in microbial isolates collected from public beaches along the Mississippi Gulf Coast. A total of 143 bacterial isolates exhibited growth and were included in antibiotic susceptibility screening. High levels of antibiotic resistance were observed, with 137 of 143 isolates showing growth on vancomycin, 124 on amoxicillin, and 102 on erythromycin. Resistance to kanamycin (19/143 isolates) and tetracycline (33/143) was less common. Multidrug resistance was widespread, with 101 isolates (70.6%) resistant to three or …

Skeletal Muscle Characteristics Long-Living Dwarf Mice In Response To High Fat Diet, Landon Schumacher, Matthew Johnston, Jaspreet Osan, Holly Brown-Borg

Arts & Sciences Undergraduate Showcase

Introduction: The effects of high fat diet (HFD; 60% kcal: fat) on skeletal muscle in mice with postnatal growth hormone deficiency is relatively unknown. We compared the tibialis anterior (TA) muscle from middle aged female Ames Dwarf and Wildtype mice fed HFD for 16 weeks and standard diet (STD; 10%: fat) to see the impacts of a HFD on inflammation and fibrotic formation in the musculature.   Methodology: Mice were euthanized after diet duration (age 10-12 months), and TAs were collected and frozen in OCT for cross-sectional cryosection. Ten um thick muscle was fixed to slides and stained with Oil Red-O …

From Sunshine To Sight Lost: Amd In North Dakota, Sidney J. Leciejewski, Diane Darland

Arts & Sciences Undergraduate Showcase

Age-related macular degeneration (AMD) is a progressive eye disease that directly harms the macula, which could eventually cause vision loss. The macula is an area on the back of the eye that contains cone cells, which allow for our color vision. Its location also allows us to have our central vision. There are two kinds: the dry and wet form, where the wet form is more extreme. The disease, itself, is highly prevalent in North Dakota, which is why it is important to note its causes and prevention methods. Some causes of AMD include genetic risks,  health risks, and metabolic …

Community Composition Of Antibiotic-Resistant Bacteria On The Mississippi Gulf Coast, Marisa Martin, Colin Jackson

Honors Theses

The Mississippi Gulf Coast is an important resource for transportation, economic development, and recreation. Given its economic and cultural importance, it is crucial to maintain clean and safe conditions along the coast for public health, but other than routine beach monitoring, few studies have examined microbial contamination of coastal waters in this region. This study aimed to determine the quantity, composition, and distribution of bacteria resistant to six different antibiotics (Vancomycin, Kanamycin, Tetracycline, Ciprofloxacin, Erythromycin, or Amoxicillin) in seawater at seven public beaches along the Mississippi Gulf Coast. No bacteria resistant to Ciprofloxacin were detected, and bacteria resistant to Kanamycin …

Beyond The Bug: How The Covid-19 Pandemic Exposed Longstanding Systemic Failures In The United States Workforce And Economy, Abbey M. Markley

Honors Theses

The purpose of this thesis is to investigate and discuss how longstanding systemic failures in the United States exacerbated the effects the COVID-19 pandemic had on the American economy, essential healthcare workers, and overall public trust in institutions. A review of the existing literature surrounding this topic and the subsequent results of various studies were used to compose this thesis. The United States has a painful lack of worker protections and tends to view members of its workforce as expendable, even in times such as pandemic when they are deemed essential. Healthcare workers in particular faced increasing threats to their …

Life History, Stock Status, And Movement Ecology Of Narrow-Barred Spanish Mackerel, Scomberomorus Commerson (Lacépède, 1800) In The Southern Arabian Gulf, Mohamed Abdulla Almusallami

Thesis/ Dissertation Defenses

The narrow-barred Spanish mackerel, Scomberomorus commerson, is the most commercially valuable pelagic fish species in the Arabian Gulf. This thesis integrated three studies to comprehensively assess the life history, stock status, and movement ecology of this species in Abu Dhabi waters. Life history traits were characterized from data collected during a 15-month monitoring program (January 2019–May 2020). Fork length ranged from 30.5 to 132 cm. Growth parameters were k = 0.14–0.15 year⁻¹ and L∞ = 128.9–135.4 cm for males and females, respectively. Size-at-maturity (Lm₅₀) was 78.7 cm FL for females and 69.7 cm FL for males. The spawning season occurred …

Faith, Science, And Choice: Vaccine Attitudes Among Religious University Students, Jamie Jensen

ScholarsArchive Data

These data comprise a Q-methodology study conducted at Brigham Young University among undergraduate students to determine vaccine attitudes in relation to religious and political ideologies. It includes raw sort data, templates for running Q-methodology, and interview transcripts.

The Longevity Of Dna From Bloodstains And Its Forensic Implications, Nadia Walter

Senior Honors Theses

Human DNA is valuable forensic evidence, helping to solve a wide range of cases. It is often sourced from biological fluids found at a scene such as blood, saliva, semen, and urine. Many different factors can influence the longevity of DNA sourced from bloodstains, including the type of substrate, storage conditions, and weather. The quality of a DNA sample from a bloodstain found at a crime scene may influence its admissibility in court.

Original research was done on a surgical table from the last major battle of the American Civil War, extracting DNA from latent bloodstains. Some full profiles were …