Digital Commons Network^™

Enabling Better Interoperability And Reusability For Down Syndrome Transcriptomic Datasets, Harrison Oaks, Garrett L. Wride, Stephen Piccolo

Library/Life Sciences Undergraduate Poster Competition 2025

Introduction: Gene expression profiling has become a cornerstone of biomedical research, offering relevant insights into the molecular mechanisms that underlie complex conditions such as Down syndrome (DS), a condition caused by trisomy 21. RNA expression data from individuals with DS have highlighted critical pathways involved in development, immune function, and disease susceptibility. Despite the availability of over 100 publicly shared RNA expression datasets, their potential remains underutilized due to inconsistent metadata, diverse normalization protocols, and varying data quality. Addressing these barriers is vital to enabling researchers to standardize, integrate, and analyze datasets effectively. We do this by creating a data …

Effects Of Predation On Cognition In Brachyrhaphis Rhabdophora, Elizabeth F. Jenkins, Jerald B. Johnson

Library/Life Sciences Undergraduate Poster Competition 2025

Does predation environment affect the cognitive abilities of the tropical fish Brachyrhaphis rhabdophora?

Barks Vs Bears: A Ruff Reality, Fern Luttrell, Tom Smith, Stephen Herrero, Lana Ciamiello, Hank Hristienko, Erin Jacoway, Linda Wiggins

Library/Life Sciences Undergraduate Poster Competition 2025

• Will your dog protect you from a bear?
• Do dogs provoke bear attacks?
• Is it safe for your dog to be in bear country?

Assessing Sandy Beach Health Throughout The South Atlantic Bight With Ghost Crabs, Raquel Wilson, Blaine D. Griffen

Library/Life Sciences Undergraduate Poster Competition 2025

To better inform the management and protection of marine ecosystems, we examined human impacts on sandy beach health across the South Atlantic Bight by assessing the abundance and size of ghost crab burrows, and habitat quality. We used an urbanization index to measure the level of human impact. Sandy beach ecosystems are ecologically and economically important, yet they face immense pressure from increasing human activity^1,2. Protecting these ecosystems is critical yet challenging due to their large size, but we can evaluate their health and the extent of human impact using a bioindicator species. We used the Atlantic ghost …

Those Dam Fish: Morphological Response In Two Riverine Fish Species Following Dam Construction, Leah Stimpson, Austin Rejcek, Mark C. Belk

Library/Life Sciences Undergraduate Poster Competition 2025

Flow regime is a known predictor of fish shape¹. Conspecific populations living in distinct flow environments often exhibit different morphologies.

Dams present a unique opportunity to observe a dramatically transformed flow regime. Fish populations that once occupied a fast-flowing habitat suddenly live in an artificial reservoir.

The Parana Basin in southern Brazil boasts some of the greatest fish diversity in the world. The biological implications of hydroelectric dam construction along its major rivers must be studied.

Previous literature has been limited to a single species or ecological niche².

➢ Our objective was to describe what morphological …

I’M Just Like You: Do Sympatric Lizard Species Exhibit Convergent Morphology? (Genus Sceloporus), Abigail Lamartine, Alison Whiting

Library/Life Sciences Undergraduate Poster Competition 2025

The spiny lizards, genus Sceloporus, are the most speciose genus of reptiles endemic to North America. These lizards are commonly seen doing “push-ups” and head bobs, showing off their bright blue belly and throat patches (figure 5).Sceloporus use these displays to attract mates and defend their territory, and the size and color of patches is the most commonly used character to identify species(see figure 3). Within southwestern Utah, there is a small region where two species, S.occidentalis and S. tristichus, overlap in range (figure 1), and lizards can be found that appear to have patches intermediate between the two …

Frog Barcodes: Scanning The Secret Species Of Pelodryadinae, Kennedy Hunter, Taylor Probst, Alison Whiting

Library/Life Sciences Undergraduate Poster Competition 2025

Tree frogs (Hylidae) have unique adaptations such as toe pads for life in the trees (figure 1).

The subfamily Pelodryadinae is poorly known and primarily found in the Australopapuan region.

Papua New Guinea is the island with the greatest frog diversity but many species remain undescribed.

DNA barcoding has been shown to be an effective tool in species delimitation and for examining species diversity in poorly understood groups.

Epigenetic Reprogramming In Pediatric Tumors Predicts Active Pathways In The Tumor Microenvironments, Noah Moffat, Clark Tolbert, Colin Wessman, Matthew H. Bailey

Library/Life Sciences Undergraduate Poster Competition 2025

Figure 1: We collected samples from five different studies that analyzed and generated spatial transcriptomic data from adult and pediatric brain tumors1-4.

Understanding The Individual Behavioral Variation Of Asian Elephants (Elephas Maximus) As An Indicator Of Human Elephant Conflict, Julia Brzezicki

Masters Theses & Specialist Projects

Human-Elephant Conflict (HEC) is an increasingly common event that arises from resource competition due to overlaps in human and elephant environments. Using a conservation behavior approach, the behaviors of elephants can be studied to enhance conservation efforts to mitigate HEC by better predicting which elephants engage in HEC. To understand the factors that affect individual behavioral types of adult Asian elephants (Elephas maximus), my project examined the behavioral patterns displayed by an elephant population in Sri Lanka. I collected demographic and behavioral data of a subset of 15 adult elephants to determine individual differences in five behavioral categories: Affiliation, aggression, …

A Systematic Study Of The Highly Endemic Spider Beetle Fauna (Coleoptera: Ptinidae: Ptininae) Found In The Coastal Deserts Of Perú, Marlena Askren

Masters Theses & Specialist Projects

Spider beetles are small, often flightless insects that occur in hyperarid environments around the world. They exhibit interesting morphology and behavior that allows them to conserve water they obtain from plant material, dung, and other detritus. One hyperarid region in which they are found is the Pacific coast of Perú and northern Chile. The Sechura Desert, Peruvian Coastal Desert, and Atacama Desert make up the arid coast where spider beetles occur from north to south. Through multiple years of field work and museum collections, populations of spider beetles were found along the coast in isolated pockets of vegetation, separated by …

Biocultural Mobility: The Impact Of Osteoarthritis On Rural Kentuckians, Alexandria Anderson

Mahurin Honors College Capstone Experience/Thesis Projects

Osteoarthritis (OA) is a common chronic degenerative joint disease that results in joint pain and loss of joint function, and it is rising in prevalence alongside an aging population. OA has a complex etiology, with various biological, mechanical, and sociocultural factors affecting its acquisition and progression. Like most diseases, OA has a differential prevalence among populations; it is disproportionally more common in underserved communities, including rural areas. This thesis presents (1) a study comparing selected OA treatment type and patient travel time, (2) a study comparing OA comorbidity amount and rurality, and (3) a discussion of exploratory interdisciplinary methods in …

Characterization Of Legionella Pneumophila Effector Protein Ceg10, Emmanuella Ofoka

Masters Theses & Specialist Projects

Legionella pneumophila, the causative agent of Legionnaires' disease, utilizes a Type IV secretion system (T4SS) to transport effector proteins into host cells. This bacterium is said to have over 300 effector proteins. Among these, the Ceg10 effector protein had remained uncharacterized, despite evidence suggesting the importance of bacterial effector proteins in the host-pathogen interaction. This study aimed to perform a structural and functional characterization of Ceg10 through transcriptomic, bioinformatics, and molecular analyses. Transcriptomic profiling of HEK 293T cells expressing Ceg10 revealed significant differential gene expression, with approximately 20 upregulated genes, including leucine-rich repeat, as well as other downregulated genes. …

Learning In Nonleafcutting Fungus-Gardening Ants, Elizabeth D. Boshers

Biology Theses

Social complexity influences learning and other cognitive behaviors, but for insects, this influence is not well understood. Delayed rejection is an aversive learning behavior previously studied exclusively in leafcutting ant species, the most socially complex of the fungus-gardening ants. Fungus-gardening ants are unique in that they do not directly consume the material they forage, using it instead as substrate to cultivate a symbiotic fungus on which the ants are obligately dependent. Here, I attempt to induce delayed rejection in several fungus-gardening ant species that exhibit a range of social complexity, then compare the characteristics of delayed rejection behavior among species. …

Acoustic Monitoring Of Oak Toads (Anaxyrus Quercicus), A Longleaf Pine (Pinus Palustris) Ecosystem Endemic Amphibian, Alex Ward Foote

Theses, Dissertations and Capstones

The Longleaf pine ecosystem is one of the most endangered ecosystems in the world with only 3% remaining. Many endemic species to the longleaf pine ecosystem are also in decline and suffer from habitat loss resulting from fire exclusion, urbanization, agriculture, and industrial silviculture. Oak Toads (Anaxyrus quercicus) are a longleaf pine ecosystem obligate; the species is experiencing declines across its range and only has protections in Virginia and North Carolina. We used data from an array of Automated Recoding Units across four years (2013, 2014, 2015, 2019) at three state-owned wildlife management areas to capture audio files …

A Metadata Analysis Of Unionid Mussels Of The Greenup Pool Of The Ohio River Using Occupancy Modeling And Species Area Curves, Tyler Mitchell Annarino

Theses, Dissertations and Capstones

Although North America contains the largest diversity of freshwater mussels in the world, only ~24% of species in the United States and Canada have stable populations. This group provides unique hurdles for surveying, and current unionid survey protocols include neither longitudinal studies nor randomization of sites. This project conducts a metadata analysis of freshwater mussels in the Greenup Pool of the Ohio River (GPOR) to answer three questions: 1. Does a combination of longitudinal scope, randomization of sites, and historic methodology of surveying result in useful occupancy/detection models (ODMs) of unionids in large streams at the family level? 2. Does …

Propelling Whole-Cell Biosensor Creation By Leveraging Gene Fusion To Assemble Synthetic Functional Transcription Factors For The Detection Of Biomarkers, Samuel J. Verzino

Theses and Dissertations

Biosensors are analytical devices that leverage biological modules for the detection of molecules of interest. As customizable tools designed to aid in a wide array of diagnostics, these instruments are rapidly advancing the field of synthetic biology. While varied in their construction and purpose, biosensors are ultimately concerned with converting a physicochemical stimulus into a detectable signal. Among the many existing variants, whole-cell biosensors (WCBs) stand out due to their versatility and engineering potential. WCBs are a type of biosensor in which a physiologically active cell sustains an array of biomolecular elements - receptors, transporters, regulators, etc. - capable of …

Analysis Of The Influence Of Telomere Length Variation On Flowering Time And Plant Fitness In Arabidopsis, Brianna Spadavecchia

Theses, Dissertations and Capstones

Telomeres are conserved structures at the ends of eukaryotic chromosomes that promote genome stability. In most eukaryotes, the DNA found within these protective end caps consists of short, G-rich nucleotide repeats. Cells with critically short telomeres face a variety of potential adverse outcomes such as end-to-end chromosome fusion, apoptosis, etc. The length of telomeric DNA varies substantially between different plant species and even between genotypes of the same species. A previous study using T-DNA mutants of the model plant Arabidopsis thaliana with long or short telomeres suggested that telomere length may be an adaptive trait. Specifically, for reproductive fitness, the …

Cold Atmospheric Plasma Induced Degradation Of Organophosphate Pesticides On Kevlar Swatches, Ta Chun Lin, Victor Somtochukwu Mbanugo, Boluwatife Stephen Ojo, Yue-Wern Huang, Marek Locmelis, Frank Daoru Han

Biological Sciences Faculty Research & Creative Works

Cold atmospheric plasma (CAP) was evaluated for degrading organophosphate pesticide (OP) residues (acephate, malathion, and dimethoate) from Kevlar fabrics. Fourier transform-Infrared (FTIR) spectroscopy, scanning electron microscope (SEM) imaging and tensile strength testing confirmed that CAP treatment preserved Kevlar's structural integrity and mechanical properties. Results show degradation efficiency increased with higher power, shorter discharge gaps, and longer exposure duration. Hyperspectral imaging supported the detection of plasma-induced spectral changes, reinforcing the presence of reactive species. The strong oxidative potential of CAP facilitated the rapid breakdown of OPs into nontoxic byproducts. These findings demonstrate the potential of CAP as a portable, energy-efficient solution …

Climate And Gypsum Parent Material Shape Biocrust Communities And Moss Ecology In The Chihuahuan And Mojave Deserts, Katelyn Gobbie, Nicole Pietrasiak, Brian M. Jusko, Rebecca E. Drenovsky

2025 Faculty Bibliography

Biological soil crust communities (biocrusts) establishing on gypsum soils have been well-documented for their prolific appearance and rich diversity of lichens and bryophytes. However, studies characterizing gypsum biocrusts have occurred primarily outside of the U.S., most of which lack comparisons to other soil types. We conducted intensive field surveys to evaluate the ground cover and frequency of biocrust functional groups and moss species on gypsum and non-gypsum soils in the U.S. regions with the most extensive gypsum outcrops, the northern Chihuahuan and eastern Mojave Deserts. Study sites were stratified by geomorphology and paired, so that every gypsum site was matched …

Friend And Foe: Tgf-Beta, Its Role In Tumor Development, And An Analysis Of Chung Et Al. (2018), Yosef Sonbolian

The Science Journal of the Lander College of Arts and Sciences

This paper will analyze an important study performed by Chung et al. (2018) regarding transforming growth factor-beta (TGFbeta), which plays an important role in various cellular processes. Paradoxically, TGF-beta plays a different role in the development of cancer at various points. In early-stage tumors, TGF-beta has been known to inhibit cancer growth, but in later stages, it acts as a cancer promoter. Chung et al. (2018) attempted to ascertain the efficaciousness of the drug Sorafenib in suppressing TGF-beta responses, as well as the ways that this drug achieves its goal and the implications that their findings may have for future …

Validation Of Non-Invasive Methods For The Measurement Of Gonadal And Inter-Renal Steroid Hormones In A Desert-Adapted Amphibian (Scaphiopus Couchii), Alexander T. Baugh, Callie Cho , '23, Alice Onyango-Opiyo , '23, Sophie A. Rodner , '25, Senna Mieth , '25, Daniel Oakes , '24, Liam Halstead , '25

Biology Faculty Works

For aquatic and semi-aquatic vertebrates like amphibians, it is possible to estimate excreted hormone levels using non-invasive methods such as waterborne and salivary sampling. These techniques allow monitoring of endocrine activity over varying, repeated and simultaneous integration periods while minimizing handling-related stress that can ‘contaminate’ hormone estimates, including estimates of baseline glucocorticoids. Here we have validated the extraction and quantification of three steroid hormones (corticosterone, CORT; 17-b estradiol, E₂; testosterone, TST) in Couch’s spadefoots (Scaphiopus couchii)—a desert-adapted anuran of special interest for physiology, evolution and conservation—using non-invasive waterborne and minimally invasive salivary hormone methods. We combined extraction and …

Noncoding Rnas In Nuclear Organization, Thembalami Dube , '24, Dawn M. Carone

Biology Faculty Works

No abstract provided.

Recruitment, Reprogramming, And Function Of Trpv1+ Sensory Nerves In The Development And Progression Of Head And Neck Cancer, Anthony Restaino

Dissertations and Theses

Head and neck squamous cell carcinomas (HNSCC) are an epithelial cancer prevalent across the world and are divided into two subcategories: human papillomavirus-mediated (HPV+) and carcinogen-mediated (HPV-) HNSCC. These subgroups have distinct patient populations, divergent responses to therapy, and prognoses, however the foundational mechanisms that facilitate these differences are not well understood or defined. Recently, the presence and function of sensory neurons within the tumor environment of various solid tumors has been identified and investigated. Herein, we identify differences in the presence of TRPV1+ sensory neurons and neuron structures in the tumor environment of HPV+ and HPV- HNSCC, correlating with …

Evaluation Of The Impact Of Secondhand E-Cigarette Aerosols On Ros-Mediated Alterations Of Epigenetic Events: Implications For Copd Pathogenesis And Assessment Of The Efficacy Of Cold Atmospheric Plasma In Degrading Organophosphate Pesticides On Kevlar Materials, Ta-Chun Lin

Masters Theses

"We explored 1) the toxicological impacts of e-cigarette aerosols and 2) the decontamination efficacy of Cold Atmospheric Plasma (CAP) technology over organophosphates. In examining the effects of firsthand and secondhand e-cigarette aerosol exposure on A549 and BEAS-2B lung cells, we observed significant cytotoxicity, increased ROS levels, and epigenetic modifications, including heightened DNA methylation and downregulation of histone deacetylases (HDACs). These molecular alterations may contribute to chronic respiratory conditions, such as COPD, underscoring the health risks of e-cigarette exposure.

We investigated CAP’s effectiveness in degrading organophosphate pesticide (OP) residues on fabric surfaces, revealing that CAP treatment effectively decomposes OPs such as …

Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge

Journal of Earth and Life Science

Burbot are becoming more and more popular for angling and aquaculture. However, populations have been declining across the world. Very little information is available about their early life and their interactions in the ecosystem due to challenges in sampling. Condition estimates and diet metrics were used to better understand the status of burbot in Lake Bemidji. Using backpack electrofishing, 45 young of the year burbot were collected. The lengths ranged from 86 to 146 mm and the wet weights ranged from 4.29 to 24.36 g. The stomach contents were removed and stored in ethanol for later analysis. The burbot were …

Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez

Journal of Earth and Life Science

Wild rice (mannoomin; Zizania palustris) is an aquatic grain that grows in slow-moving rivers and shallow bays throughout northern Minnesota. In Headquarters Bay on Leech Lake, wild rice has served as a cultural staple for Indigenous communities for centuries. However, its population has declined in recent years due to multiple environmental issues. One potential contributor is Eurasian watermilfoil (Myriophyllum spicatum; EWM), an invasive species that forms dense mats capable of outcompeting native aquatic vegetation, including wild rice. EWM was first recorded on the southern shoreline of Leech Lake in 2005 and has since become widespread. This study …

René Dubos: An Annotated Bibliography, Carol L. Moberg

René Dubos: An Annotated Bibliography

René Dubos (1901–1982), a prominent 20th century scientist, humanist, and philosopher, was a prolific author. Affiliated for more than 50 years with The Rockefeller Institute for Medical Research/The Rockefeller University, his studies ranged from antibiotics and the role of bacteria in human diseases to formulating broad ecological and environmental questions affecting human health and disease. His seminal writings explore a lifelong belief that a living organism—whether microbe, human being, society, or the Earth itself—could be understood only through its relationships with everything else.

This comprehensive bibliography of more than a thousand works by René Dubos includes brief annotations for each …

The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei

Journal of Earth and Life Science

Zebra mussels Dreissena polymorpha are an aquatic invasive species that has been spreading quickly throughout Minnesota water bodies since 1989. Zebra mussels are known to change certain morphological conditions in lakes such as turbidity, phytoplankton abundance, and aquatic invertebrate populations. However, studies showing impacts of the infestation of zebra mussels on the health of a fish community have been hard to prove due to a lack of repeated surveys and a suite of other confounding variables such as natural and seasonal variability, plant community, and lake morphometry. Therefore, the objective of this study is to determine whether zebra mussels have …

Analysis Of Recreational Data On Nearby Rivers, Rachael Meyers, Tamara Johnstone-Yellin

ASPIRE 2025

In the 1980’s. DuPont of Waynesboro, VA, contaminated over 100 miles of river on the South Fork of the Shenandoah and the South River. As part of their settlement, creel surveys are completed routinely by the Department of Wildlife Resources to collect data on angler and recreational use of these rivers. This project uses the recreational data to determine; ‘where on the South Fork of the Shenandoah River is recreational access the heaviest?’ ‘what forms of recreational use are most popular, and is there a change from 2016 to 2023?’ and ‘are there differences in the demographics of recreational users …

Size Of Age-0 Sander Vitreus Pre And Post Dreissena Polymorpha Infestation On Brainerd And Bemidji Area Lakes, Quady Bernu

Journal of Earth and Life Science

Walleye Sander vitreus is the Minnesota state fish and a vital predator fish in lake ecology. Zebra mussels, Dreissena polymorpha were first found in Minnesota inland lakes in the 1980’s. These mussels have had huge impacts on water clarity as well as phytoplankton and zooplankton population levels which affect how age-0 fish feed, especially a low light feeding predator fish like walleye. A study took place on age-0 walleye size and it supported the conclusion that age-0 walleye were negatively impacted by zebra mussels in Minnesota’s large lakes. The goals of this study were to determine how the size of …