Digital Commons Network^™

Fta™ Card Use For Detection Of Mycoplasma Gallisepticum In Wild Birds, Linda Purvis, Dawn E.W. Drumtra

Georgia Journal of Science

Flinders Technology Associates (FTA) cards are increasingly accepted as an accurate method for nucleic acid detection, including testing for the presence of various avian diseases. However, the accuracy of FTA cards for detection of Mycoplasma gallisepticum (MG) in wild birds has not been published. Traditional techniques for testing MG in birds include serum plate agglutanation (SPA), hemagglutination inhibition (HI), polymerase chain reaction (PCR), and enzyme linked immunosorbent assay (ELISA) all of which are time consuming and require significant financial investment into specialized equipment. We tested the efficacy of using FTA cards to detect MG in wild bird carcasses collected in …

Development And Characterization Of Sodium Alginate-Based Active Edible Films Functionalized With Olive Mill Wastewater Extract, Nassima Hadri, Mohamed Didi Ould El-Hadj, Zineb Mahcene, Fatih Bozkurt, Rusen Metin Yildirim, Youcef Rahmani, Aicha Tedjani, Muhammet Arici

Karbala International Journal of Modern Science

Phenolic compounds from olive mill wastewater (PCO) of Algerian origin were used to produce sodium alginate-based active films using the casting method. The effects of adding various concentrations of PCO (0%, 0.1%, and 0.2% w/v) were evaluated regarding the molecular, morphological, thermal, physicochemical, optical, barrier, biodegradability, antimicrobial, and antioxidant properties of the alginate films. The FTIR and SEM results elucidated the development of a coherent cross-linked structure attributed to hydrogen bonding interactions between PCO and alginate chains. Consequently, the films exhibited enhanced crystallinity and thermal stability, as revealed by DSC analysis. Moreover, PCO addition positively influenced several film properties, including …

Acute Toxicity Of Chlorimuron-Ethyl To Daphnia Magna, Jack H. Bagwell

SPARK Symposium Presentations

The acute toxicity of a sulfonylurea herbicide, chlorimuron ethyl, to Daphnia magna was determined experimentally using acute toxicity tests.

On Solving Differential Equations Describing The Ecology Of Shallow Lakes: A Comparison Of Classical And Modern Computational Methods Through Python, Vruddhi Kapre, Jeffrey R. Anderson, Alessandro Maria Selvitella

Spora: A Journal of Biomathematics

In this exposition paper, we describe some computational methodologies to solve numerically differential equations modeling the shallow lake ecosystem, with particular focus on the dynamics of the phosphorus. We describe in detail Python implementations of classical algorithms, like Runge-Kutta, and more modern approaches, including physics-informed neural networks.

Circadian And Age-Related Variations Of Amino Acids Levels In Drosophila Brains: Correlations And Descriptive Dimensions, Sandrine Parrot, Jacob Crehan, Chloé Aman, Philippe De Deurwaerdère, Matthew S. Thimgan, Laurent Seugnet

Biological Sciences Faculty Research & Creative Works

A comprehensive view of whole-brain amino acid levels holds the potential to provide valuable insights into the brain's state, given the mutual interconnections through metabolism, food intake, and neurotransmission. We tested this concept by evaluating free amino acid levels in single Drosophila brains across 24 h and at two different ages. A large proportion of these amino acids displayed time-of-day variations, and a subset exhibited age-dependent variations. Cross-correlation analysis of the data sets confirmed broad time-of-day and age dependent interconnections between amino acids. Factor Analysis of Mixed Data revealed further data structuration along key amino acids. For example, 50% of …

Dataset Supporting "Functional Trait Responses Of Alternanthera Philoxeroides To Increasing Salinity: Stress Tolerance, Avoidance And Escape Strategies Support Invasiveness With Sea Level Rise, Brenda J. Grewell*, Blanca Gallego-Tévar, Jesús M. Castillo, Caryn J. Futrell, Rebecca E. Drenovsky, Nathan E. Harms, Paul D. Pratt

Alligator Weed Salinity Exp for NeoBiota

No abstract provided.

Neuroma Prevention By Nerve To Artery Coaptation: A Pilot Study, Tyler D. Ragsdale, Peter K. Firouzbakht, Benjamin A. Nwadike, Grant R. Kolar, John Tracy Watson, Vikas Dhawan

Biological Sciences Faculty Research & Creative Works

Purpose: Neuroma formation is a notable complication that can occur following amputation. Newer techniques are proving to be successful in preventing neuroma formation by providing the proximal nerve segment with an end organ to help with organized growth. Redirecting arterial blood flow to the nerve endings may be an effective mechanism of preventing neuroma formation. The aim of this study was to demonstrate that by coapting the proximal end of a transected nerve with a functional artery as a vascular end organ, neuroma formation can be prevented. Methods: Six Sprague-Dawley rats were used for the control and experimental groups. Subjects …

Bioinformatics Analysis Of The Mbl2 Missense Variants Associated With Cystic Fibrosis, Elaine Vanterpool, Kiar-Ra Cameron

Student Posters

Cystic fibrosis (CF) is an autosomal recessive disease commonly recognized by thick mucus and loud coughs. The manifestation of these symptoms is due to the inability of chloride ions to diffuse out of the cell. Thus, preventing osmosis resulting in a thick mucus on the lung’s surface. Those who suffer from this disease have difficulty breathing and require a modulator with a vest to increase gas exchange in the lungs. Moreover, the disease can result in much pain due to coughs, which destroys ciliated epithelial cells. Patients who have cystic fibrosis can suffer from pneumonia and other bronchial infections. They …

Pregnancy And Postpartum Effects Of Electronic Cigarettes On Maternal Health And Vascular Function In The Fourth Trimester, Amber Mills, Duaa Dakhlallah, Anand Ranpara, W. Travis Goldsmith, Paul D. Chantler, Yue-Wern Huang, Jonathan Boyd, I. Mark Olfert

Biological Sciences Faculty Research & Creative Works

Pregnancy is a vulnerable time with significant cardiovascular changes that can lead to adverse outcomes, which can extend into the postpartum window. Exposure to emissions from electronic cigarettes (Ecig), commonly known as "vaping," has an adverse impact on cardiovascular function during pregnancy and post-natal life of offspring, but the postpartum effects on maternal health are poorly understood. We used a Sprague Dawley rat model, where pregnant dams are exposed to Ecigs between gestational day (GD)2-GD21 to examine postpartum consequences. Litter and dam health were monitored during the weaning period, and maternal vascular and endocrine function were assessed after weaning. Exposure …

Investigating The Impact Of Roundup® Exposure On Hearing In Channel Catfish (Ictalurus Punctatus), Joshua W. Adams, Daniel E. Holt

CSU Tower Day

Tower Day 2025 Undergraduate Poster 3rd place Winner

Glyphosate-based herbicides (GBHs) such as Roundup® are used extensively worldwide, yet their potential to disrupt fish sensory physiology has remained relatively unexplored. Previous studies have established that glyphosate exposure can induce various neurotoxic effects in fish and other vertebrates—ranging from oxidative stress to altered neurotransmitter systems and anxiogenic-like responses. Despite these documented impacts, no published research has directly assessed whether glyphosate compromises auditory function in fish.

Here, we address this knowledge gap by examining the hearing sensitivity of farm- raised Channel catfish (Ictalurus punctatus) following a 96-hour exposure to Roundup® (3 mg/L …

Measuring Spatial And Temporal Variation In Species Composition Of Ephemeral Pond Amphibian Assemblages Using Metabarcoding, Elizabeth Strasko

Masters Theses & Specialist Projects

Amphibian populations worldwide face severe threats, heightening the need for reliable monitoring methods. In this thesis, I explored and tested environmental DNA (eDNA) metabarcoding protocols to survey amphibian assemblages in ephemeral ponds of the the Green River Preserve in central Kentucky, USA. First, I evaluated whether the commonly used mitochondrial cytochrome c oxidase subunit I (COI) gene could effectively capture species-level variation in local amphibians for eDNA metabarcoding. Extensive primer design and testing revealed significant challenges in amplifying fragmented eDNA from water samples. In a second study, I applied a metabarcoding approach using the mitochondrial 12S ribosomal RNA (12S) gene …

A Phylogeographic Study Of The Kentucky Endemic Cave Beetle Neaphaenops Tellkampfii Erichson (1844), Mahamad Miya

Masters Theses & Specialist Projects

Cave beetles are an excellent group to study evolutionary history and speciation by isolation within cave habitats. However, they are underrepresented in such studies, particularly in North America, one of the hotspots of cave biodiversity where beetles account for a significant proportion. The present research addresses this issue by focusing on the Kentucky endemic cave beetle, Neaphaenops tellkampfii, a species hypothesized to comprise four subspecies. Based on allozyme data, previous studies have noted high genetic variation within and high similarity among local populations of this beetle, suggesting complex evolutionary dynamics. In this regard, this study aims to elucidate the evolutionary …

Mutations In The Pms1 Gene Associating With Ovarian Cancer, Elaine Vanterpool, Erin Hough

Student Posters

The female reproductive system contains two ovaries on each side of the uterus, producing eggs and hormones such as estrogen and progesterone. Ovarian cancer occurs when abnormal cells in the ovaries grow and divide uncontrollably, destroying healthy body tissue. Common symptoms of ovarian cancer include weight loss, fatigue, discomfort in the pelvic area, or changes in bowel habits. The goal of this research is to understand and identify the pathogenicity of the PMS1 gene in ovarian cancer and factors associated specifically with the mutation.

The Influence Of Mef2c Variants On Autism Development, Elaine Vanterpool, Maya O’Reilly

Student Posters

Autism Spectrum Disorder (ASD) is a condition that affects how people communicate, interact, and behave, with about 1 in 54 children in the U.S. being diagnosed. This study looks at the MEF2C gene and its possible link to ASD.

Does Digital Screen Exposure Have An Impact On The Ocular Health Of Children And Adolescents?, Hannah Brodt

The Science Journal of the Lander College of Arts and Sciences

The increasing prevalence of digital screens among children and adolescents raises significant concerns regarding their ocular health. This paper reviews current evidence linking screen time to visual health issues, particularly myopia (nearsightedness), dry eye syndrome, and digital eye strain. Through a comprehensive literature review of observational studies, the analysis identifies a strong correlation between excessive screen time and the exacerbation of myopia and dry eye disease, particularly in younger populations. The findings highlight the importance of balanced screen time and suggest that early exposure to screens may significantly increase the risk of developing myopia. Further research is also recommended to …

Which Of The Environmental Aspects Affecting The Gut Microbiome Of The Neonate Has The Largest Lifelong Impact?, Roza Heershzon

The Science Journal of the Lander College of Arts and Sciences

The development of new DNA sequencing techniques has led to new discoveries on the gut microbiome’s critical influence on metabolic processes and immune development. Therefore, understanding the most influential factors in the development of the gut microbiome during early infancy, which impacts the individual’s long-term health, is vital. This paper explores how environmental factors, including delivery method and nutrition, shape the gut microbiome’s devel- opment in early life. In addition, it discusses their long-term health implications. The paper highlights how the mode of delivery significantly affects initial microbial colonization. Vaginal births promote a microbial community that is rich in beneficial …

Risk Factors Of Covid-19 Mortality, Ellie Wolff

The Science Journal of the Lander College of Arts and Sciences

This paper investigates the relationship between key health demographics and COVID-19 mortality while focusing on identifying both physiological and genetic factors that contribute to variations in mortality rates. Specifically, it examines how pre-existing conditions—such as sex, autoimmune disorders, cardiovascular diseases, and obesity—interact with COVID-19 pathophysiology to influence patient outcomes. While previous research has highlighted demographic disparities in hospital mortality rates, this study aims to establish a causal, mechanistic link between these health factors and COVID-19 severity. Methods to extract and analyze data included literature review using existing studies such as peer-reviewed articles and reports from PubMed, CDC, etc. By analyzing …

Investigating The Interactions Between Elm1 And Hsl7 In The Morphogenesis Checkpoint Of Saccharomyces Cerevisiae, Rachel Foster

Masters Theses & Specialist Projects

Cell shape is essential to understanding the normal regulatory processes that occur within the cell and can indicate there are abnormalities present when there is dysfunction in the cell's development, which can have broader implications regarding human disease. The morphogenesis pathway couples cell division to the timely formation of a daughter bud in Saccharomyces cerevisiae and consists of Elm1, Hsl1, Hsl7, and Swe1 proteins which interact with septins at the bud neck to provide stability for the bud and to facilitate cell division. Elm1 is needed for the recruitment of Hsl1 and subsequently Hsl7. Hsl7 will then tether Swe1 to …

Examining The Probiotic Potential Of Pseudoalteromonas Rubra Situated In The Scholarly And Public Spheres, Sam Ruemmler

Honors Senior Capstones and Theses

Climate change is affecting our oceans negatively in many ways, such as rising temperatures and increased disease outbreaks. While there are many diseases that are affecting marine organisms, bacteria of the genus Vibrio are present globally and are known to favor warmer waters, which means increasing temperatures will cause an uptick in infections. One species, V. coralliilyticus, has been identified as a pathogen responsible for coral disease and death as well as mortality in rainbow trout, brine shrimp, and Pacific oyster larvae. A possible solution to the rising prevalence of disease is the use of probiotics. One such probiotic candidate …

The Impact Of Large, Manipulated Rainfall Events On Soil Greenhouse Gas Fluxes Is Mediated By Warming, Spatial Heterogeneity And Hurricane Disturbance Recovery In A Puerto Rican Wet Tropical Forest, Nibia Becerra Santillan

Biology Honors Projects

Substantial uncertainty surrounds how terrestrial ecosystems may respond to the interaction of multiple global change drivers, especially the interaction of atmospheric warming and more frequent or severe extreme weather events. Climate models suggest that the Caribbean may experience more frequent and/or heavier rainfalls under future climate, which is likely to also interact with increased atmospheric temperatures. In this thesis, I investigate how long-term (“press”) soil moisture and temperature conditions interact with acute (“pulse”) extreme precipitation events to influence CO₂ fluxes, labile carbon, and microbial carbon in a wet tropical forest in Puerto Rico. I conducted a fully-factorial soil incubation experiment …

Mutations In The Mmp20 Gene In Association With Amelogenesis Imperfecta, Elaine Vanterpool, Alicia Broomfield-Myrie

Student Posters

Enamel, the hardest substance in the body, protects the teeth from various forces like temperature and physical factors that would cause damage. Depending on the kind of AI one has developed, the phenotypes can range from thin teeth to teeth discoloration or decay. MMP20, a gene commonly associated with AI, encodes for Enamelysin, a protein crucial for enamel formation. It is also known as matrix metallopeptidase 20. It becomes active when it is cleaved by cellular proteases. When this gene is mutated, the splicing pattern is altered. Consequently, this can cause disease. This study was developed to analyze the pathogenicity …

Drone Thermal Imaging And Benthic Time-Series Analysis Show Dynamic Spatial And Temporal Delivery Of Submarine Groundwater Discharge On Reef Ecosystems, Sophia G. Zummo, Florybeth F. La Valle, Lucian Himes

Faculty Data Sets

Submarine groundwater discharge (SGD) introduces nutrient-rich, low-salinity water into coastal ecosystems, significantly altering reef biogeochemistry. At Black Point, Oʻahu, we employed a novel, two-pronged approach that integrates a cost-effective small unmanned aerial system equipped with a thermal infrared (sUAS-TIR) sensor and high-resolution benthic salinity time series to resolve previously unobserved, fine-scale patterns of SGD delivery. sUAS-based sampling overcomes key limitations of prior SGD mapping methods, such as labor-intensive and spatially constrained in situ sampling, by enabling rapid deployment, real-time visualization of mixing dynamics, and high-resolution imagery. Unlike previous studies that relied on customized systems that required independent sensor integration, our …

Evolution Of Chitinases In Amphibians: A Multi-Faceted Group Of Enzymes With Dual Function In Digestion And At The Forefront Against Fungal Infections, Devashri Narayan

Theses and Dissertations

Chitinases are essential enzymes found in living organisms such as amphibians, and they ensure that amphibians survive in their varied ecosystems. They are crucial in the digestion and immunity of most organisms, including plants and animals. Chitinases’ diversity of functions is a testament to their evolutionary adaptability. The primary function of chitinases is the breakdown of chitin, a complex polymer of saccharides often found in the exoskeleton of insects and the walls of fungi. Amphibians that feed on insects make use of chitinases to hydrolyze chitin into simpler sugars that can be easily absorbed to extract the necessary nutrients from …

Under Stress: Maternal Effects On Offspring Development In Live-Bearing Fish, Ruby Jensen, Emily Mead, Jerald B. Johnson

Library/Life Sciences Undergraduate Poster Competition 2025

Research has shown that the stress response in adult fish can lower sex hormone levels, disrupt mating behavior, and reduce overall reproductive success (1). While stress is known to impact an individual's ability to reproduce, its effects on offspring after birth remain unclear. Specifically, the influence of maternal stress during gestation on offspring growth and maturation is still unknown.

To better understand how maternal stress affects offspring development, we will study the live-bearing fish Alfaro cultratus from birth to reproductive maturity. We will observe the timeline of developmental milestones in offspring whose mothers experience stress during gestation to assess potential …

Effect Of Isostatic Rebound On Soil Biodiversity, Robert Candland, Blake Harrison, Solinus Farrer, Kyle Lake, Byron Adams

Library/Life Sciences Undergraduate Poster Competition 2025

Purpose: Understand the effect of shifting tide lines on organism diversity and abundance

Ms Environmental Biology Capstone Project, Emily A. Patton

Regis University Student Publications (comprehensive collection)

This is a masters Thesis for MS in Environmental Biology containing four chapters including a literature review, grant proposal, journal manuscript, and stakeholders report on a current environmental issue. (1) Noise Pollution and Avian Communication in Urban Ecosystems: When the Natural World Struggles to Hear Itself. Urban noise masks essential bird vocalizations, which harms avian fitness. Most species are displaced, and those few urban birds which counteract this noise with song adaptations likely experience trade-offs. America's noise policy remains undeveloped, threatening the health of both humans and avian wildlife (2) Application of Noise Barrier Technology for the Restoration of Urban …

Aligning Exons, Introns, And Flanking Regions Of Cyc And Cuc Genes In Lonicera (Caprifoliaceae), Mark Mordukhay

Theses and Dissertations

The genus Lonicera (Caprifoliaceae) exhibits great diversity in floral symmetry, petal fusion, and organ arrangement, making it an ideal system for exploring the genetic basis of morphological evolution. We focus here on the roles of CYC and CUC gene families, (CYC2A, CYC2B, CYC3A, CYC3B, CUC2A, and CUC2B) and their potential contributions in shaping floral symmetry and organ fusion. The data set included nucleotide sequences from over 90 Lonicera species, extracted from samples collected from natural habitats or herbaria and then sequenced. A custom bioinformatics pipeline was developed in R using Bioconductor packages to automate BLAST searches, filter results, scan for …

The Involvement Of Camp In The Secretion Of Il-10, Jyanna J. Mendola

SURF Posters 2025

Schwann cells have been found to play a key role in inflammation and repair following nerve injury. During nerve injury, the myelin sheath becomes damaged, stimulating Schwann cells to secrete cytokines and initiate an inflammatory response. They begin the process of repair by stimulating macrophages to make pro-inflammatory cytokines, such as Tumor Necrosis Factor-Alpha (TNF-a), and anti-inflammatory cytokines, such as Interleukin 10 (IL-10) which promotes healing and injury. Although it is clear Schwann cells play a role in nerve repair, there is still yet to be known regarding the exact mechanisms by which they do so. In vitro, Lipopolysaccharide (LPS), …

Ludwigia Hydrology Study, Rebecca E. Drenovsky, Rebecca Reicholf, Caryn J. Futrell*, Brenda Grewell*, Blanca Gallego-Tévar

Ludwigia Hydrology Study

Premise: Hydrological regime influences wetland plant species distribution and performance.Global warming and extreme weather events are magnifying flooding patterns, and understanding how invasive taxa respond across life stages (establishment vs. established phase) is important for predicting and managing their colonization and spread. Our objective was to measure flood trait responses at contrasting life stages in closely related congeners (Ludwigia peploides, diploid; L. hexapetala, decaploid; Onagraceae) differing in their invasiveness in the field.

Methods: In the field, we assessed phenological responses to seasonal hydrological changes, and in mesocosms, we assessed flood stress responses of establishing shoot fragments under deep-flooded, …

Ms Environmental Biology Capstone: Evaluating Environmental Risks And Conflicts In Golf Course Landscapes, Meredith Boos

Regis University Student Publications (comprehensive collection)

1) This review investigates the relationship between pesticide use on golf courses and in agriculture to determine whether the adverse health effects documented in agricultural settings also apply to golf course environments. While the negative health effects from agricultural pesticide use are well established, golf courses use pesticides with greater frequency and intensity, suggesting a potentially higher exposure risk for both workers and players.

2) This study proposes methods to quantify pesticide bioaccumulation on golf courses and adjacent lands. We hypothesize that areas surrounding golf courses will exhibit a spatial gradient of pesticide accumulation, increasing with proximity to treated sites …