Digital Commons Network^™

Testosterone Amplifies The Negative Valence Of An Agonistic Gestural Display By Exploiting Receiver Perceptual Bias, Nigel K. Anderson, Martina Grabner, Lisa A. Mangiamele, Doris Preininger, Matthew J. Fuxjager

Biological Sciences: Faculty Publications

Many animals communicate by performing elaborate displays that are incredibly extravagant and wildly bizarre. So, how do these displays evolve? One idea is that innate sensory biases arbitrarily favour the emergence of certain display traits over others, leading to the design of an unusual display. Here, we study how physiological factors associated with signal production influence this process, a topic that has received almost no attention. We focus on a tropical frog, whose males compete for access to females by performing an elaborate waving display. Our results show that sex hormones like testosterone regulate specific display gestures that exploit a …

Geometric Morphometric Analyses Define Riverine And Lacustrine Species Flocks Of Himalayan Snowtrout (Cyprinidae: Schizothorax) In Nepal, Binod Regmi, Marlis R. Douglas, David R. Edds, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Freshwater fishes in the river and lake systems in the Himalayas and Tibetan Plateau are morphologically diverged but the evolutionary relationship of putative subspecies separated in these freshwater systems has not been explored. Snowtrout (Schizothorax spp.) are minnows (Cyprinidae) broadly distributed in Asia. Body shapes of 3 Lake Rara (northwest Nepal) endemics (S. macrophthalmus, S. nepalensis, S. raraensis) and 2 widely distributed riverine species (S. progastus, S. richardsonii) across 3 drainages in Nepal (i.e. Karnali, Gandaki, and Koshi Rivers) were studied using geometric morphometry. Data were derived from museum voucher specimens/tissues collected …

Elucidation Of Multi-Origin Inula Helenium L. For Antimicrobial Activity And Heavy Metal Profiling As An Exemplar For The Use And Regulation Of Plants As Antibiotics., Ciara Ruth Kenny

Theses

An impending post-antibiotic era looms, thus new sources of therapeutic chemical entities are crucial to sustain effective antimicrobial chemotherapy worldwide. Plant natural products are a source of accessible, structurally diverse compounds that provide therapeutic potential. The pharmacological applications of plants in medicine can be guided by the attestation of traditional use or the application of this knowledge to uncover new leads in the drug discovery process. The range of modified in vitro methods commonly used to investigate preclinical antimicrobial efficacy of plant-derived natural products, and the associated limitations and challenges in the provision of new antimicrobial drugs from plants is …

Molecular Responses To Catastrophic Molting In A Wild Marine Mammal, Anna Keith

University of the Pacific Theses and Dissertations

While most mammals shed their hair and skin either continuously or seasonally, northern elephant seals (Mirounga angustirostris) undergo an annual catastrophic molt, in which they shed their entire fur and underlying skin layer in the span of just three weeks. Due to the energetic and thermoregulatory constraints of molting and the large distances between their coastal rookeries and foraging grounds, elephant seals must remain on land and fast for the duration of their molt. Previous studies of molting northern elephant seals have examined endocrine and metabolic adjustments to fasting, but not the molecular processes underlying molting. We examined changes in …

Identification And Sequence Analysis Of Set Superfamily Genes In Hymenopteran Insects, Tanima Joshi

Scripps Senior Theses

Post-translational chemical modifications of histones, the proteins that package DNA, are a key component of the “language” of epigenetics. SET (Suppressor of Variegation 39, Enhancer of Zeste, Trithorax) domain-containing proteins are crucial enzymes that establish histone methylations as a major part of the histone code. Studies using the fruit fly, Drosophila melanogaster, as a model organism have provided most of the known information on SET genes/protein diversity and function in insects. To expand our knowledge of this important protein family, I identified all SET domain-containing genes in a select group of insects belonging to the Hymenoptera order: wasps, bees, ants, …

Landscape Genetics Of Iowa's Threatened Black Redhorse, Morganne C. Borsh

Honors Program Theses

The objectives of this study are to:

a. Characterize the genetic diversity of populations of Black Redhorse in Iowa

b. Determine the level of connectivity between populations in Iowa

Overall, this project aims to determine the degree of fragmentation of Black Redhorse populations to better direct conservation strategies. This study utilizes genome-wide Single Nucleotide Polymorphisms (SNPs) to assess the population genetic structure of the Black Redhorse across its range in northeast Iowa.

Changes In The Vertical Stratification Of Neotropical Nymphalidae At Forest Edges In Relation To Light And Temperature Conditions, Brian Koji Oye

University of the Pacific Theses and Dissertations

The composition of neotropical fruit-feeding nymphalid butterflies assemblages often varies by location and stratum within a forest. Recent work has shown that vertical stratification in particular may serve as barrier to gene flow, indicating the potential role vertical stratification may play in evolution. At forest edges, the distinction between assemblages has been described to decrease, with species considered to be canopy specialists descending to the forest understory. The similarity in light conditions between the canopy and understory strata at edges or disturbed habitat is hypothesized to be responsible for this phenomenon. We conducted a study using standardized sampling to document …

Creating A Georgia Southern Spider Collection: Can Dna Barcoding Help?, Guy B. Hobbs

College of Graduate Studies: Theses & Dissertations

With over 280 spider (Araneae) species recorded within the State of Georgia, USA, the need for a well-documented natural history collection with a usable voucher system is critical to continually assess spider diversity and their future ecological impact in this region. Spider identification can be daunting for the inexperienced taxonomist; it is time consuming and sometimes requires destructive procedures. Previous works have successfully used an alternative method, DNA barcoding, to correctly identify spider species while preserving their morphology. This study set forth to create the core of a well-documented spider collection within Georgia Southern University’s Institute for Coastal Plain Science …

Novel Quantification And Localization Of Water And Solute Transporters In The Tissues Of The Spiney Dogfish (Squalus Acanthias), Tolulope B. Ojo

College of Graduate Studies: Theses & Dissertations

The dogfish, Squalus acanthias is a marine cartilaginous elasmobranch found in the North Atlantic and Pacific oceans. Dogfish synthesize and excrete urea as a product of nitrogen metabolism. They also convert ammonia into urea and retain this urea, such that their plasma is isosmotic or slightly hyperosmotic to the surrounding seawater. To facilitate the regulation of body fluid and to maintain ionic concentrations and osmotic pressure, Dogfish use ion and solute transporters (e.g., NCC, UT-1) and aquaporin water channel proteins. Studies have identified some of the aquaporin genes in the elasmobranch genome, but their functions are mostly uncharacterized. Recent transcriptomic …

Free-Roaming Cat Abundance Across A Habitat Gradient, Rachel E. Bird

College of Graduate Studies: Theses & Dissertations

There are an estimated 172 million owned and feral cats in the United States, and wildlife enthusiasts and cat owners are often at odds over how best to manage free-roaming cats. Management is needed because of the documented impacts of free-ranging cats on wildlife. Targeting these management efforts, however, is hampered by an imperfect understanding of cat distribution in the landscape. My study used game cameras and capture-recapture sampling to estimate abundance of free-roaming cats across a habitat gradient in Bulloch County, Georgia, USA. In all, I detected cats at 51% (25/49) sites with a mean of 2.1 cats per …

Temporal Variation In The Genetic Composition Of An Endangered Marsupial Reflects Reintroduction History, Rujiporn Thavornkanlapachai, Harriet R. Mills, Kym Ottewell, J. Anthony Friend, W. Jason Kennington

Research outputs 2014 to 2021

The loss of genetic variation and genetic divergence from source populations are common problems for reintroductions that use captive animals or a small number of founders to establish a new population. This study evaluated the genetic changes occurring in a captive and a reintroduced population of the dibbler (Parantechinus apicalis) that were established from multiple source populations over a twelve-year period, using 21 microsatellite loci. While the levels of genetic variation within the captive and reintroduced populations were relatively stable, and did not differ significantly from the source populations, their effective population size reduced 10–16-fold over the duration of this …

Habitat Selection By Vulnerable Golden Bandicoots In The Arid Zone, Cheryl A. Lohr, Kristen Nilsson, Colleen Sims, Judy Dunlop, Michael T. Lohr

Research outputs 2014 to 2021

In 2010, vulnerable golden bandicoots (Isoodon auratus) were translocated from Barrow Island, Western Australia, to a mainland predator-free enclosure on the Matuwa Indigenous Protected Area. Golden bandicoots were once widespread throughout a variety of arid and semiarid habitats of central and northern Australia. Like many small-to-medium-sized marsupials, the species has severely declined since colonization and has been reduced to only four remnant natural populations. Between 2010 and 2020, the reintroduced population of golden bandicoots on Matuwa was monitored via capture–mark–recapture data collection, which was used in spatially explicit capture–recapture analysis to monitor their abundance over time. In 2014, we used …

Describing A Putative Corollary Discharge Circuit In Drosophila, Kaitlyn Nicole Boone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Corollary discharge (CD) circuits provide critical information about movement and behavior to provide context to sensory processing. However, to date, there has not been a comprehensive study of CD circuits at a single-cell level. In this thesis, I aimed to resolve the connectivity of ascending histaminergic neurons, consisting of two pairs, the mesothoracic pair (MsAHNs) and metathoracic pair (MtAHNs) at a single-cell level and characterize contexts of activation. Using transgenic techniques, connectomics and transcriptomics, we identify neural populations receiving input from the AHNs and neural populations with significant output to the AHNs. We explored where the AHNs predominantly receive synaptic …

Documentation Of Atlantic Tarpon (Megalops Atlanticus) Space Use And Move Persistence In The Northern Gulf Of Mexico Facilitated By Angler Advocates, J. Marcus Drymon, Matthew B. Jargowsky, Michael A. Dance, Mitchell Lovell, Crystal L. Hightower, Amanda E. Jefferson, Andrea M. Kroetz, Sean P. Powers

University Faculty and Staff Publications

Atlantic tarpon (Megalops atlanticus, hereafter tarpon) are facing a multitude of stressors and are considered Vulnerable by the IUCN; however, significant gaps remain in our understanding of tarpon space use and movement. From 2018 to 2019, citizen scientists facilitated tagging of 23 tarpon with SPOT tags to examine space use and movement across the northern Gulf of Mexico. Movement-based kernel densities were used to estimate simplified biased random bridge-based utilization distributions and a joint move persistence model was used to estimate a behavioral index for each fish. Tarpon showed consistent east–west movement from the Alabama/Florida border to Louisiana, and utilization …

Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg

Murray State Theses and Dissertations

The differences in energetic input between the sexes required to produce gametes translates to the differences in reproductive behavior and overall mating systems seen in a species. Females generally produce a few energetically and resource expensive eggs and typically choose a high-quality suitor to ensure she has high quality offspring. In contrast, males produce abundant energetically cheap sperm and attempt to fertilize as many eggs as possible in as many females as possible. Both sexes are trying to maximize their inclusive fitness, but the dichotomy of interests can lead to sexual conflict and perhaps extreme or unusual behaviors such as …

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …

An Evaluation Of College Preparedness On Natural Selection Principles Following The Completion Of An Entry-Level Biology Course, Amy Bell

Murray State Theses and Dissertations

Abstract

Evolution education in secondary education has long been a topic of research. The level of knowledge and acceptance of students upon entering college has been studied using various methods; however, no study had provided the perception of preparedness from the student perspective nor had analyzed the individual Natural Selection principles. This study analyzed college freshmen (n=162) in an entry-level BIO 101 course. Participants were given the Conceptual Inventory of Natural Selection Instrument (CINSI) and perception survey questions upon completion of the course. The CINSI data was then analyzed for each of the four Natural Selection principles as well as …

A Fresh Start In Ambersnail (Gastropoda: Succineidae) Taxonomy: Finding A Foothold Using A Widespread Species Of Oxyloma, Kathryn E. Perez, Marco A. Martinez Cruz, Brent W. Steury, Gary M. Barker

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The ambersnails (Succineidae), found nearly worldwide, are considered a very challenging group to classify and identify with even genus-level identifications requiring dissection. In this study, we use mitochondrial and nuclear DNA markers, shell morphometrics, and anatomical dissection to examine fresh material collected from the type localities of two nominal species in Oxyloma (Neoxyloma). We conclude from these evidence that Oxyloma salleanum (L. Pfeiffer, 1850) and Oxyloma effusum (L. Pfeiffer, 1853) are conspecific, and accordingly reduce O. effusum to the status of junior synonymy. We present a redescription of O. salleanum.

Factors Driving The Compositional Diversity Of Apis Mellifera Bee Venom From A Corymbia Calophylla (Marri) Ecosystem, Southwestern Australia, Daniela Scaccabarozzi, Kenneth Dods, Thao T. Le, Joel P. A. Gummer, Michele Lussu, Lynne Milne, Tristan Campbell, Ben Pan Wafujian, Colin Priddis

Research outputs 2014 to 2021

Bee venom (BV) is the most valuable product harvested from honeybees ($30 - $300 USD per gram) but marginally produced in apiculture. Though widely studied and used in alternative medicine, recent efforts in BV research have focused on its therapeutic and cosmetic applications, for the treatment of degenerative and infectious diseases. The protein and peptide composition of BV is integral to its bioactivity, yet little research has investigated the ecological factors influencing the qualitative and quantitative variations in the BV composition. Bee venom from Apis mellifera ligustica (Apidae), collected over one flowering season of Corymbia calophylla (Myrtaceae; marri) was characterized …

Demography, Morphometrics, And Stomach Contents Of Common Ravens Examined As A Result Of Controlled Take, Corinne M. Gibble, Kriss K. Neuman, Jessie Beck

Human–Wildlife Interactions

Common ravens (Corvus corax; ravens) are known nest predators that have the ability to negatively impact nesting birds, including imperiled species of seabirds and shorebirds. We conducted systematic necropsies of ravens that were lethally controlled in Monterey Bay, California, USA during 2013–2015, in or near western snowy plover (Charadrius nivosus nivosus) nesting areas, in an effort to better understand body condition, overall health, and diet of individual ravens. Raven predation of snowy plover nests has increased over the years in the Monterey Bay study area, and lethal removal of ravens has been employed to reduce predation. …

Effects Of Wolf And Grizzly Bear Recovery On Cougars In A Multi-Use System, Jennifer A. Feltner

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

A Reintroduction Retrospective: Comparative Fisher (Pekania Pennanti) Survival And Prey Habitat Use In The Cascade Mountains Of Washington State, Tanner S.T. Humphries

Graduate Student Theses, Dissertations, & Professional Papers

Increasing human impacts on biodiversity highlight the global need for ecological restoration. For many wildlife species, reintroduction is necessary to re-establish populations in parts of their historic range where they have been extirpated. Reintroduction efforts are commonly used to help restore ecosystem integrity, but are often expensive, time consuming, and unsuccessful at generating self-sustaining populations. Thus, a more complete understanding of the factors affecting restoration success is important for ensuring successful outcomes and responsible stewardship. Fishers (Pekania pennanti) are one of the most commonly reintroduced carnivores in North America, but the success of such efforts is highly variable, …

Comparison Of Standard And Environmental Dna Methods For Estimating Chinook Salmon Smolt Abundance In The Klamath River, California, Doyle Joseph Coyne

Cal Poly Humboldt theses and projects

Evaluating abundance of juvenile salmonids is critical to conservation and management. Current abundance estimation involves use of rotary screw traps and mark-recapture studies. Use of environmental DNA (eDNA) in water samples offers a noninvasive and less expensive approach that may potentially improve or eventually replace traditional monitoring. Here I evaluate the utility of eDNA to predict weekly abundance estimates of outmigrating Chinook salmon (Oncorhynchus tshawytscha) smolts in the Klamath River, California. A total of 15 water samples were collected per week over the 17-week smolt outmigration in both 2019 and 2020. Chinook salmon eDNA concentration in each water …

Predator Scent And Presence Alter Mammal Assemblages In The Missouri Ozarks, Usa, Cara Jean Yocom-Russell

Masters Theses

"Species that are vulnerable to predation exhibit a host of behavioral and physiological adaptations toward the avoidance of this outcome: Heightened awareness of surroundings through visual, olfactory, and auditory senses are common ways in which these species avoid detection by predators. While links between direct predator-prey relationships are well established, less is known about how predators can shape overall community structure or the populations of secondary or less frequently consumed prey items. As humans expand into rural areas, the frequency of wildlife conflicts rises. In response, humans look to prevent these events with a variety of methods. One such method …

Functional Genetic Approaches To Provide Evidence For The Role Of Toolkit Genes In The Evolution Of Complex Color Patterns In Drosophila Guttifera, Mujeeb Olushola Shittu

Dissertations, Master's Theses and Master's Reports

Toolkit genes are set of genes that orchestrate the development of basic body plan of animals, and they are highly conserved in all animals. The co-option of the toolkit genes into the pigmentation pathway has led to the evolution of novel species. This study focuses on understanding how the complex color patterns in animals develop by using the Drosophila species in the quinaria group as models. We developed an mRNA in situ hybridization (ISH) protocol, which allowed us to study gene expression patterns in the abdomen of developing pupae of non-model Drosophila species (Chapter 2). Through ISH, we found that …

Arthropod Fauna Of Plains Pocket Gopher (Geomys Bursarius) Burrow Systems, Brett Lyle Gourley

Dissertations and Theses @ UNI

Pocket gophers are among the most asocial mammals, living a solitary existence except for mating and the rearing of offspring. They create expansive burrow systems underground while foraging for food and rarely surfacing above ground. As gophers burrow, excess soil is pushed to the surface or placed in older tunnels. This behavior creates a cave-like ecosystem where pocket gophers and associated arthropods live.

Few studies have been done on the arthropod fauna cohabitating with pocket gophers in these burrows. Most of these studies have focused on a family of arthropods over several sites. Many have focused on the southern United …

Neuroprotective And Neurotherapeuticeffects Of Dihydrotestosterone,17Β-Estradiol, And Pueraria Mirifica Extract On Cognitive Impairment In Androgen Deficient Rats, Taratorn Fainanta

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aims to understand the mechanism of action of androgen deficiency, which usually occurs in elderly men, and the neuroprotective and neurotherapeutic effects of dihydrotestosterone (DHT), 17β-estradiol (E2) and Pueraria mirifica extract (PME) on cognitive impairment in male rats. Four series of experiments were conducted. Experiment I was to understand the mechanism of action of androgen deficiency on cognitive impairment. Rats at the age of 4 months old were orchidectomized (ODX) or sham-operated (SH), kept for 3 days, 2, 4, 6, and 8 months (M0, M2, M4, M6 and M8, respectively), determined spatial learning behavior and memory capacity, and …

Investigating The Antitumor Effects Of A Dsrna-Nanoparticle Complex In An In Vitro Ovarian Cancer Model, Aaron Lewis

Theses and Dissertations (Comprehensive)

An estimated 1 in 70 women will be diagnosed with ovarian cancer in their lifetime. Despite advanced detection and treatment methods, it remains a silent killer with an expected survival rate of 50%. A developing method in cancer treatment is the use of compounds that stimulate the immune system to aid in the body's fight against the disease. This project focused on the use of the potent immune stimulant double-stranded RNA (dsRNA), commercially available as polyinosinic:polycytidylic acid, poly(I:C), to induce cytotoxicity in two ovarian cancer cell lines; SKOV-3 and OVCAR-3. Some challenges exist with the delivery of dsRNA due to …

Dsrna Conjugated Phytoglycogen Nanoparticles As An Innate Immune Stimulant To Prevent Viral Infections In Rainbow Trout And Atlantic Salmon, Kayla Samms

Theses and Dissertations (Comprehensive)

Salmonids are one of the world’s most farmed fish species. These aquatic vertebrate species rely heavily on their innate immune responses to defend themselves against invading pathogens. Although commercial vaccines are available against some viral and bacterial pathogens affecting salmonids, their protective efficacy is variable. Using a prophylactic inducer of local and systemic innate immune responses could have significant implications in salmonid aquaculture. A potent inducer of the innate immune response in fish is double-stranded RNA (dsRNA), a molecule that all viruses make during their replicative cycle. Polyinosinic:polycytidylic acid (polyI:C) is a synthetic dsRNA commonly used to induce type I …

Composition And Homology In The Taxonomic Classification Of Escherichia Coli, Tanya Irani

Theses and Dissertations (Comprehensive)

As new techniques have been introduced, specifically the possibility of complete genome sequencing, better methods of defining bacterial species have also been proposed. One of the most recently proposed methods, using bioinformatic techniques, is to calculate the average nucleotide identity (ANI) between the homologous genome segments of different isolates. Another method for species discrimination that has been tested successfully is the similarity of DNA compositional signatures. However, in a recent update, DNA signatures split the available Escherichia coli complete genomes into three groups. To check if this result was consistent with such genomes belonging to different species, we tested methods …