Digital Commons Network^™

Deoxybuzonamine Isomers From The Millipede Brachycybe Lecontii (Platydesmida: Andrognathidae), Tappy H. Jones, Daniel P. Harrison, Carla Menegatti, Emily Levers, Kenneth Knott, Paul Marek, Derek A. Hennen, Matt T. Kasson, Angie M. Macias, Brian Lovett, Ralph Saporito

2022 Faculty Bibliography

Millipedes (Diplopoda) are well known for their toxic or repellent defensive secretions. Here, we describe (6aR,10aS,10bR)-8,8-dimethyldodecahydropyrrolo[2,1-a]isoquinoline [trans-anti-trans-deoxybuzonamine (1a)] and (rel-6aR,10aR,10bR)-8,8-dimethyldodecahydropyrrolo[2,1-a]isoquinoline [trans-syn-cis-deoxybuzonamine (1b)], two isomers of deoxybuzonamine found in the chemical defense secretions of the millipede Brachycybe lecontii Wood (Colobognatha, Platydesmida, Andrognathidae). The carbon–nitrogen skeleton of these compounds was determined from their MS and GC-FTIR spectra obtained from the MeOH extract of whole millipedes, along with a subsequent selective synthesis. Their structures were established from their 1D (¹H, ¹³C) and 2D NMR (COSY, NOESY, multiplicity-edited HSQC, HSQC-TOCSY, HMBC) spectra. Additionally, computational chemistry (DFT and DP4) was used to identify the relative …

Alkaloid-Based Chemical Defenses In 11 Species Of Bufonid Poison Frogs From Brazil (Bufonidae: Melanophryniscus), Emily Staufer

Masters Theses

The term ‘poison frog’ describes members of five anuran families that are capable of sequestering lipophilic, alkaloid-based chemical defenses from their prey. Alkaloid defenses can vary widely within and among poison frog species, mostly linked to differences in availability of arthropod prey at different scales. While research has mainly focused on alkaloid variation within the dendrobatid and mantellid poison frogs, bufonid poison frogs in the genus Melanophryniscus demonstrate high inter- and intra-species alkaloid variation, and some members are capable of both sequestering dietary alkaloids and synthesizing alkaloids metabolically. However, of the 31 species of Melanophryniscus, only eight have ever had …

Comparison Of Effects Of Sleep Fragmentation On Pro-Inflammatory Cytokine Il-1Β Of Male And Female Adult Mice, Manzar Rzayeva

Mahurin Honors College Capstone Experience/Thesis Projects

Sleep plays an essential role throughout the body by affecting the physiological function and regulation of many systems. One of these systems that receives effects as a result of the adequacy of sleep is the immune system. Previous studies have demonstrated effects of sleep fragmentation upon the immune system; however, sexual differences of these effects have not been studied in depth. To analyze these variances amongst the genders, male and female adult mice were subjected to acute sleep fragmentation (SF) for 24 hours in an automated SF cage that includes a bar sweeping across the cage every two minutes. Meanwhile, …

Crossmodal Perception Of Object Shape: A Study On The Effect Of Modal Order On Successful Shape Recognition, Ashlyn Vale

Mahurin Honors College Capstone Experience/Thesis Projects

Visual and haptic (tactile) modes of perception, while occasionally exercised independently, most often occur concurrently. The degree to which the ordering of the two modes of perception affects successful recognition of three-dimensional shapes varies. Some have found that the cross-modal orders (vision followed by haptics or vice versa) produce equal performance (Caviness, 1964; Lacey, Peters, & Sathian, 2007; Norman et al., 2006), while other researchers found visual-haptic (VH) performance to be superior to haptic-visual (HV) performance (Davidson, Abbott, & Gershenfeld, 1974; Norman, Clayton, Norman, & Crabtree, 2008). The current experiment used an old-new recognition task (with cookie cutter stimuli). In …

Ms Environmental Biology Capstone Project, Samantha Ortega

Regis University Student Publications (comprehensive collection)

This project is presented in partial fulfillment of the requirements for the degree Masters of Science in Environmental Biology at Regis University. Chapter 1 is a literature review that discusses the management implications of designing bird habitat on anthropogenic structures. Chapter 2 is a grant proposal to explore niche dominance by anthropophilic bird species in Denver, CO. Chapter 3 is a journal manuscript discussing how musth impacts captive bull Asian elephant (Elephas maximus) behavior at Denver Zoo. Finally, Chapter 4 is a stakeholder analysis for Northern Bobwhite recovery at Bridgestone-Firestone Centennial Wilderness Area, White County, Tennessee.

What Is The Best Method To Cure Hiv?, Israel Hacker

The Science Journal of the Lander College of Arts and Sciences

Human Immunodeficiency Virus (HIV) is a sexually transmitted retrovirus impacting millions of people worldwide. Strides have been made to cure and/or prevent various genetic mutations via gene editing. Scientists hope to rid of HIV on a genomic level which will also prevent Acquired Immunodeficiency Syndrome (AIDS) - a biproduct of HIV. HIV utilizes a mechanism that counteracts the innate, adaptive, and intrinsic immune systems. Because the virus’s replication takes place in the lymphatic organs, the infection traverses the entire bloodstream. Due to the complexity of HIV and AIDS, their constant evolution, and their effect on a genomic level, no cures …

Can Stem Cell Transplantation Restore Insulin Levels In Diabetics?, Nechama Mendelson

The Science Journal of the Lander College of Arts and Sciences

A recent public interest in stem cell research has led to new approaches to treat various pathologies, including diabetes. Diabetes mellitus is characterized by elevated blood glucose levels due to the inability of the β cells in the pancreas to produce insulin. Therefore, the standard diabetes treatment is introducing insulin to the bloodstream. With the advancement of stem cell therapy, a new approach to treating diabetes mellitus is being researched. Scientists are working to differentiate pluripotent stem cells into mature insulin-producing pancreatic beta cells. These cells are then transplanted into in vivo models and observed after a glucose challenge for …

Ecological Risk Assessment For The Western Rock Lobster Resource : Workshop Procedure And Outcomes, R Stoklosa

Fisheries Occasional Publications

No abstract provided.

Isolating Bacteriophage For Potential Treatment Of Chronic Multi-Drug Resistant Escherichia Coli Infections, Leila Oswalt

Honors Projects

The misuse and overuse of antibiotics has led to the intense rise in antibiotic resistance. As society transitions into the post antibiotic era, there will be a great need for new therapeutic strategies to address multiple drug resistant bacterial infections. One such method, called bacteriophage therapy, allows for specific targeting of certain pathogenic bacteria through the use of viruses that attack bacteria; termed “bacteriophage” or simply “phage”. Urinary tract infections are among the most common pathological human infections that rely heavily on the use of antibiotics, the major cause of which is the bacterium Escherichia coli. During the Spring 2021-Spring …

Study Of Zyomogen Granule Movement Along Actin Filaments Using A Single Beam Optical Trap, Justin James Raupp

Wayne State University Dissertations

Zymogen granules are enzymatic vesicles in the pancreas. The surface of these zymogen granules (ZGs) has several different kinds of myosin molecules, such as myosin 1c, 6, 5c, and 7b. These molecular motors may contribute to ZG transportation in cells. To understand the molecular motors involved in the vesicle trafficking, we observed the in vitro motility of purified ZGs from rat pancreas and examined the stepping behavior and force that is generated using a single beam optical trap. To be involved in trafficking, molecular motors have certain characteristics, a high duty ratio and the ability to move continuously along actin …

Spatial And Temporal Variability Of Mercury In Upper And Lower Red Lake Walleye, Tyler Orgon

Biology Graduate Theses

Mercury is a global pollutant that is released into our environment by natural and anthropogenic processes resulting in extensive studies conducted on mercury cycling in aquatic ecosystems which has led to the issuance of human-health-based fish-consumption advisories. We examined total mercury concentrations in Walleye Sander vitreus from Upper and Lower Red Lakes, located in north central Minnesota, between 2019 and 2020. Upper and Lower Red Lake form a contiguous water body consisting of two large (483.1 and 665.6 km2, respectively) basins that are naturally connected by a 1.4 km-wide strait. Both basins are important fisheries for recreation, subsistence, and commercial …

The Nutritional Condition Of Moose Co-Varies With Climate, But Not With Density, Predation Risk, Or Diet Composition, Jennifer S. Forbey, Daniel P. Melody

Biology Faculty Publications and Presentations

A fundamental question about the ecology of herbivore populations pertains to the relative influence of biotic and abiotic processes on nutritional condition. Nutritional condition is influenced in important, yet poorly understood, ways by plant secondary metabolites (PSMs) which can adversely affect a herbivore’s physiology and energetics. Here we assess the relative influence of various abiotic (weather) and biotic (intraspecific competition, predation risk and diet composition) factors on indicators of nutritional condition and the energetic costs of detoxifying PSMs for the moose population in Isle Royale National Park (U.S.A.). Specifically, we observed interannual variation in the ratio of urea nitrogen to …

Ecology Of Black-Legged Ticks (Ixodes Scapularis) At Fort Drum Military Installation, New York, Lucas Eli Price

Graduate Theses, Dissertations, and Problem Reports (ETD)

The number of confirmed cases of Lyme disease in the United States of America has risen from 12,801 cases reported in 1997, which is the earliest year these data are available, to 23,453 confirmed cases in 2019. In the eastern United States, black-legged ticks (Ixodes scapularis) are the primary vector of Lyme disease, which is caused by the spirochete bacterium, Borrelia burgdorferi. Tick abundance tends to be highest in dense forest habitats and where wildlife diversity is low. Because wildlife species have varying levels of competency as reservoir hosts, sources of tick bloodmeals influence Lyme disease ecology. …

A Combinatorial 5-Htr Expression Pattern Within The Ventral Projection Neurons Of The D. Melanogaster Olfactory Circuit., Mohd Freezely Ezzani Bin Mazri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neuromodulation allows neurons within a circuit to respond to stimuli from the environment according to the correct ecological value, context, and internal state of the animal. Serotonin (5-HT) is a neuromodulator that can generate different outcomes based on its target’s serotonin receptor (5-HTR) expression by affecting secondary messenger cascades. Within the Drosophila olfactory system, ventral projections neurons (vPN) express all five insect 5-HTR that project into two olfactory processing regions, the antennal lobe (AL) and the lateral horn (LH). The significance of this 5-HTR expression is unknown. In this study, I theorized the patterns of 5-HTR expression of vPNs. I …

New Records Of Digenetic Trematodes Infecting Melanoides Tuberculata (O.F. Müller, 1774) In Florida, Usa, Lori R. Tolley-Jordan, Michael A. Chadwick, Jimmy K. Triplett

Research, Publications & Creative Work

Melanoides tuberculata is a well-known invasive, freshwater snail and intermediate host for many species of parasitic trematodes. Despite being well established in Florida’s freshwater systems, little work has been conducted to document the associated trematodes of the snails. Live snails were collected from 11 sites from 25 locations sampled across the state of Florida, USA of which individuals from six sites were found to be infected with trematodes. Gross morphological characters and phylogenetic analysis using 18S rRNA and ITS2 gene regions, were used to identify these trematode taxa. Snails infected with the gill flukes, Centrocestus formosanus and Haplorchis pumilio (Heterophyidae) …

Assessing The Effects Of Age And Sex On Mtbi Severity, Jennifer Harrell

Honors Academy

“Mild Traumatic Brain Injury (mTBI) accounts for 70-90% of TBIs recorded in the last two decades. Subjection to continued mTBIs could result in severe comorbidities appearing years later. Current research on mTBI shows significant male sex and single age skew in murine animal models. Using the Missouri Blast Model, mice were inflicted with mTBI and evaluated using standard behavior tests and a novel method not previously used for mTBI with an open blast model. With both methods, a larger clinical difference was found between age groups than sex. We were also able to show that the novel tracking method was …

"Reeling In" Juvenile Sportfish Through Coastal Habitat Restoration: Population, Community, And Trophic Responses In The Indian River Lagoon, Florida, Jennifer Loch

Electronic Theses and Dissertations, 2020-2023

Coastal habitats provide crucial nursery habitat for predatory fishes, but they are in decline worldwide, impacting economically important fisheries. Habitat restoration can simultaneously mitigate the effects of habitat loss and benefit predators (e.g., sportfish), although this relationship is understudied. Here, the response of juvenile sportfish to oyster reef and living shoreline restoration is compared to controls in the Indian River Lagoon, Florida prior to and following restoration for up to three years through examination of community (diversity, assemblage), population (abundance), biometric (size, body condition), ontogenetic, and trophic (gut contents, stable isotopes) dynamics. Stable isotopes were used to quantify dietary history, …

Distribution And Breeding Of The Black-Bellied Whistling-Duck (Dendrocygna Autumnalis) In Arkansas, With A Brief Chronology Of Distribution And Breeding In Southeastern United States, C. Renn Tumlison

Journal of the Arkansas Academy of Science

The Black-Bellied Whistling-Duck (Dendrocygna autumnalis) was a rare bird first reported from Arkansas in 1982, when it was seen in Hempstead Co. in southwestern Arkansas. Observations surged in parts of the state about 2005, then again about 2016, and the bird is now fairly common seasonally in many parts of the state. By use of online sources for citizen science, we elucidate the history of occurrence and present analysis of seasonal distribution of this bird in Arkansas. Individuals occur in Arkansas throughout the year, but observations are most common and widely distributed from March-September, after which most individuals …

Effects Of Clipping Of Flight Feathers On Resource Use In Gallus Gallus Domesticus, Renee Garant, Bret W. Tobalske, Neila Bensassi, Nienke Van Staaveren, Dan Tulpan, Tina Widowski, Donald R. Powers, Alexandra Harlander-Matauschek

Faculty Publications - Department of Biological & Molecular Science

Ground-dwelling species of birds, such as domestic chickens (Gallus gallus domesticus), experience difficulties sustaining flight due to high wing loading. This limited flight ability may be exacerbated by loss of flight feathers that is prevalent among egg-laying chickens. Despite this, chickens housed in aviary style systems need to use flight to access essential resources stacked in vertical tiers. To understand the impact of flight feather loss on chickens’ ability to access elevated resources, we clipped primary and secondary flight feathers for two hen strains (brown-feathered and white-feathered, n = 120), and recorded the time hens spent at elevated resources (feeders, …

Sentience As Part Of Emotional Lives, Frans B. M. De Waal

Animal Sentience

It is high time to explore the sentience of invertebrate animals, but this topic cannot be discussed without also exploring their emotional lives, including positive emotions. Sentience probably evolved to allow the regulation of emotions by endowing them with feelings.

Selected Dietary Plant-Based Proteins For Growth And Health Response Of Nile Tilapia Oreochromis Niloticus, Tzodoq O. Magbanua, Janice A. Ragaza

Biology Faculty Publications

Tilapia culture is one of the largest sectors of global aquaculture. Among the different species of tilapia, Nile tilapia (Oreochromis niloticus) is perhaps the top cultured species. The production of Nile tilapia has been continually increasing throughout the years resulting in genetic deterioration. Several tilapia strains with better growth performance and adaptive capability to survive in different culture conditions have been developed to alleviate the crisis. Increased demand for Nile tilapia implies higher farming cost. Plant-based proteins are utilized as partial or complete fishmeal replacements to reduce feed cost. However, these proteins can adversely affect and alter growth …

Role Of Biological Clocks In Ant Behavioral Plasticity And Parasitic Manipulation Of Ant Behavior, Biplabendu Das

Electronic Theses and Dissertations, 2020-2023

Living organisms exhibit daily rhythms as a way to anticipate predictable fluctuations in their environment. Such daily rhythmicity is the phenotypic outcome of oscillating genes and proteins, driven by an endogenous biological clock. Clock-controlled behavioral rhythms are inherently "flexible" since their phase, amplitude, and period can change throughout an animal's life hallmarked by changes in so-called chronotype. How this inherent plasticity of clock-controlled rhythms is linked to plasticity of behavior is still an open question in biology. Characterizing the various mechanistic links between plasticity of the animal clock and behavioral state will not only shed light on the molecular underpinnings …

Evaluating Exploitation Of White Crappie And Black Crappie In Three Southeast Kansas Reservoirs, Danci Lee Johnston

Graduate Theses/Dissertations

Populations of white crappie Pomoxis annularis and black crappie P. nigromaculatus were studied in three southeast Kansas reservoirs to assess exploitation and determine if current regulations were conducive for sustainable populations. Trap nets were used to sample crappie in Elk City Reservoir, Big Hill Reservoir, and Parsons City Lake. Crappie with length 210 mm and greater were tagged with FloyFD-94 tags that served as entries into a drawing for a US$300-valued reward to encourage tag reporting. Harvest and angler demographic information were collected when tags were reported. Postcard surveys and motion-sensed cameras were used in conjunction to estimate angler effort …

Land Reversion And Zoonotic Spillover Risk, John E. Vinson, Nicole L. Gottdenker, Luis Fernando Chaves, Rajreni B. Kaul, Andrew M. Kramer, John M. Drake, Richard J. Hall

Integrative Biology Faculty and Staff Publications

Deforestation alters wildlife communities and modifies human–wildlife interactions, often increasing zoonotic spillover potential. When deforested land reverts to forest, species composition differences between primary and regenerating (secondary) forest could alter spillover risk trajectory. We develop a mathematical model of land-use change, where habitats differ in their relative spillover risk, to understand how land reversion influences spillover risk. We apply this framework to scenarios where spillover risk is higher in deforested land than mature forest, reflecting higher relative abundance of highly competent species and/or increased human–wildlife encounters, and where regenerating forest has either very low or high spillover risk. We find …

Analysing Economic Costs Of Invasive Alien Species With The Invacost R Package, Boris Leroy, Andrew M. Kramer, Anne-Charlotte Vaissière, Melina Kourantidou, Franck Courchamp, Christophe Diagne

Integrative Biology Faculty and Staff Publications

1. The reported costs of invasive alien species from the global database InvaCost are heterogeneous and cover different spatio-temporal scales. A standard procedure for aggregating invasive species cost estimates is necessary to ensure the repeatability and comparativeness of studies.
2. We introduce here the invacost r package, an open-source software designed to query and analyse the InvaCost database. We illustrate this package and its framework with cost data associated with invasive alien invertebrates.
3. First, the invacost package provides updates of this dynamic database directly in the analytical environment R. Second, it helps understand the heterogeneous nature of monetary cost data for invasive …

Maternal Drivers Of Reproductive Output In The Lizard Family Scincidae: A Phylogenetic Comparative Approach, William Zang

West Chester University Master’s Theses

Scincidae is one of the most diverse families of squamates, comprising over 1,300 species of lizards with a variety of life history and ecological traits. This diversity includes three reproductive modes, where some species reproduce via oviparity (egg-laying), viviparity (live birth) or use of a mixed strategy (females switch modes). In this study, it was sought whether reproductive output differs between scincid species using these reproductive modes. Reproductive output is defined as the average product of offspring abundance and size per reproductive event, representing the reproductive effort of an individual. Additionally, the importance of phylogeny (evolutionary relatedness) and a suite …

Does The Thermal Mismatch Hypothesis Predict Disease Outcomes In Different Morphs Of A Terrestrial Salamander?, Matthew D. Venesky, Joseph Demarchi, Cari-Ann M. Hickerson, Carl D. Anthony

2022 Faculty Bibliography

Many aspects of ectotherm physiology are temperature‐dependent. The immune system of temperate‐dwelling ectothermic host species is no exception and their immune function is often down regulated in cold temperatures. Likewise, species of ectothermic pathogens experience temperature‐mediated effects on rates of transmission and/or virulence. Although seemingly straightforward, predicting the outcomes of ectothermic host−pathogen interactions is quite challenging. A recent hypothesis termed the thermal mismatch hypothesis posits that cool‐adapted host species should be most susceptible to pathogen infection during warm temperature periods whereas warm‐adapted host species should be most susceptible to pathogens during periods of cool temperatures. We explore this hypothesis using …

Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian M. Gilbert, Jossie M. Santamaria, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Lipoteichoic acid (LTA) is a cell-wall polymer in Gram-positive bacteria that stimulates inflammation. Few studies have investigated in vivo immune response to LTA, and none of the in vivo studies have been performed in birds. For this project, the pulp (a skin derivative) of growing feathers (GF) of chickens was used to investigate the in vivo effects of intradermally injected LTA. In Study 1, GF of chickens were injected with 10 μL of differing concentrations of LTA (0.1, 1.0, 10, 100 or 250 μg LTA/mL; 3 chickens/dose). Growing feathers were plucked before injection (0 h) and at 6, 24, 48, …

What Is The Recorded Economic Cost Of Alien Invasive Fishes Worldwide?, Phillip J. Haubrock, Camille Bernery, Ross N. Cuthbert, Chunlong Liu, Melina Kourantidou, Boris Leroy, Anna J. Turbelin, Andrew M. Kramer, Laura Verbrugge, Christophe Diagne, Franck Courchamp, Rodolphe E. Gozlan

Integrative Biology Faculty and Staff Publications

Invasive alien fishes have caused pernicious ecological impacts on aquatic ecosystems. However, there has not been a global appraisal of associated economic impacts. Here, we compiled reported economic impacts of invasive alien fishes using the most comprehensive global database of invasion costs (InvaCost). We analyze how fish invasion costs are distributed geographically and temporally, as well as which socioeconomic sectors are most impacted. Fish invasions have caused the economic loss of at least US$32.8 billion globally (2017 value), from only 26 reported species (of 128 known invasive alien fish species). North America had the highest costs (> 99%), followed by …

Analysing Economic Costs Of Invasive Alien Species With The Invacost R Package, Boris Leroy, Andrew M. Kramer, Anne-Charlotte Vaissière, Melina Kourantidou, Franck Courchamp, Christophe Diagne

Integrative Biology Faculty and Staff Publications

1. The reported costs of invasive alien species from the global database InvaCost are heterogenous and cover different spatio-temporal scales. A standard procedure for aggregating invasive species cost estimates is necessary to ensure the repeatability and comparativeness of studies.

2. We introduce here the invacost R package, an open-source software designed to query and analyse the InvaCost database. We illustrate this package and its framework with cost data associated with invasive alien invertebrates.

3. First, the invacost package provides updates of this dynamic database directly in the analytical environment R. Second, it helps understand the heteregoneous nature of monetary cost data for invasive …