Nsp3 Protein-Portion Interaction Of Sars-Cov-2 Using Two Yeast-Hybrid System, Ghadeer Jassem Zakareya

Selected Full-Text Master Theses 2021-

The NSP3 protein of SARS-CoV-2 plays a crucial role in the virus-host interaction during infection. This thesis explores the protein-protein interactions between NSP3 and host factors, shedding light on their significance in viral replication and pathogenesis. NSP3 is known to interact with various host proteins involved in key cellular processes, including innate immune response modulation, RNA processing and protein degradation pathways. Understanding these interactions is essential for developing targeted therapeutic strategies against COVID-19. Application of The Matchmaker GAL4Two-Hybrid Systems, to isolate the NSP3 protein and study the interactions, elucidating the NSP3 host protein interactions and attempting to identify potential key …

Exploring The Role Of Nonmuscle Myosin Ii In Renal Thick Ascending Limb Epithelial Cell Protein Trafficking And Organelle Architecture, Brook Busselman

Dissertations and Theses

The traditional functional roles of the actin associated motor protein nonmuscle myosin II (NM2) have been thoroughly studied. However, the nontraditional roles for NM2 in the context of membrane remodeling and protein trafficking events in cells have just recently been unraveled. Our work has found that genetic inactivation of NM2 protein isoforms MYH9 and MYH10 in the renal epithelium of adult mice leads to progressive kidney disease. Aberrant protein transport and localization in cells of the thick ascending limb (TAL) of the kidney nephron is accompanied by upregulation of endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) pathway. …

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …

Do The Sexually Dimorphic Biofluorescent Glands Of Plethodon Cinereus Function In Mate Choice And Are They Seasonally Regulated By Photoperiod?, Sam C. Moody-Wynn

Masters Theses

The novel biofluorescent glands of the Eastern Red-backed Salamander (Plethodon cinereus), have been shown to be sexually dimorphic where males have larger and greater numbers of glands than females. Interestingly, a seasonal pattern of increased numbers of fluorescent glands, which mirrors the peak in testosterone levels, has been described. Currently, the function and regulation of the fluorescent glands is unknown. I predicted that the glands function as a form of male ornamentation used in intersexual communication, and perhaps act as an honest signal of male quality. Even though the fluorescent glands are located on the underside of the tail, there …

Effect Of Walleye Fry Stocking On Future Gillnet Cpue, Alexander Barker

Journal of Earth and Life Science

Walleye Sanders vitreus for many states, is a key attractant of anglers from around North America. Due to immense popularity, management efforts have been conducted to maintain Walleye populations, especially in the form of stocking. The purpose of this study is to analyze the effect of Walleye fry stocking on gillnet catch per unit effort (CPUE). A total of 90 lakes were analyzed for this study within three defined regions of Minnesota, 30 from each region. The regions chosen for this study are the Minnesota Department of Natural Resources northern pike Esox lucius regulation regions which consist of the Southern, …

Lessons From An Iconic Grouper: Sex, Science, And Serendipity, Yvonne J. Sadovy De Mitcheson

Gulf and Caribbean Research

From a common little damselfish and spectacular grouper aggregations, to disappearing reef fishes and the dark side of the international seafood trade, my journey has passed through a fascinating and worrying period of change in the way we view and treat our oceans. Although much of my career has been in the Indo—Pacific, I focus here mainly on my professional ‘roots’ in the Caribbean and on the Nassau Grouper Epinephelus striatus, a species that kept me curious, taught me patience, never to assume and always to stay hopeful. This fish is almost an obsession; why can we not better safeguard …

Feeding Systems And Fresh Lamb Color Stability, Koushik Mondal

Theses and Dissertations--Animal and Food Sciences

Consumers consider the surface color of fresh lamb as the indicator of freshness and wholesomeness, which affects their purchase decisions. The redox chemistry of myoglobin (Mb) determines the color of fresh lamb. Artificial raising on milk replacer (a pre-weaning strategy) as well as red clover supplementation are management strategies used in lamb production. This thesis focuses on the effects of artificial raising on milk replacer and red clover supplementation on the color and oxidative stability of fresh lamb.

In the first experiment, the effect of pre-weaning with milk replacer on the carcass attributes, color stability and lipid oxidation of lamb …

The Morphological Evolution Of The Inner Ear In Lacertoidea (Squamata), Cristian Hernandez

Biology Dissertations - Archive

The popularization of high-resolution computed tomography(HRCT) has produced a revolution in the field of comparative anatomy. This technology allows us to reach a level of morphological detail in our observations that has never been possible previously. Now we can perform digital dissections isolating structures of interest such as individual bones, soft tissue organs, and even generate 3D models of body cavities (endocasts) such as the braincase and inner ear. Encompassed with the progress of the HRCT, 3D geometric morphometrics have emerged as a common tool to address morphological questions in a statistical context that outperforms the traditional 2D geometric morphometrics. …

The Effect Of Body Size On Mate Choice By Ambystoma Laterale Breeding With Unisexual Salamanders, Cole Christian Duvall

Senior Honors Theses and Projects

Within the genus Ambystoma exists a complex of unisexual (all female) polyploid salamanders, which can breed with males of five sexual species of salamander. Unisexuals face intense sexual selection from diploid males, who prefer to mate with females of their own species. At the Edwin S. George Reserve (ESGR), it has been observed that LLJ unisexuals with large body sizes migrate to breeding ponds earlier than small unisexuals. A possible explanation for this behavior is that large LLJ unisexuals are sexually selected against, when compared to small unisexuals. We hypothesized that A. laterale males would prefer smaller LLJ unisexuals, when …

Documenting Plant Biodiversity At The Emu-Owned Parsons Center For Arts And Sciences, Emily Edwards

Master's Theses and Doctoral Dissertations

We present the first vascular plant species checklist for the Eastern Michigan-University-owned property called the Parsons Center for Arts and Sciences. A voucher for each plant species has been deposited in the Eastern Michigan College herbarium and collection data is digitized in the Consortium of Midwest Herbaria. A minimum of three images per species are also available on iNaturalist . The Consortium and iNaturalist databases are publicly available and provide georeferenced locations for each plant. One hundred seventy-one plant species (in 137 genera and 61 families) were documented and identified, representing about 15% of plant species and 41% of families …

Lhx3/4 Initiates A Cardiopharyngeal-Specific Transcriptional Program In Response To Widespread Fgf Signaling, C. J. Pickett, Hannah N. Gruner, Bradley Justin Davidson , '91

Biology Faculty Works

Individual signaling pathways, such as fibroblast growth factors (FGFs), can regulate a plethora of inductive events. According to current paradigms, signal-dependent transcription factors (TFs), such as FGF/MapK-activated Ets family factors, partner with lineage-determining factors to achieve regulatory specificity. However, many aspects of this model have not been rigorously investigated. One key question relates to whether lineage-determining factors dictate lineage-specific responses to inductive signals or facilitate these responses in collaboration with other inputs. We utilize the chordate model Ciona robusta to investigate mechanisms generating lineage-specific induction. Previous studies in C. robusta have shown that cardiopharyngeal progenitor cells are specified through the …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Upa-Mediated Polyamidoamine Dendrimer-Based Targeted Gene Delivery System On Triple-Negative Breast Cancer, Hsin-Yin Chuang

Masters Theses

"Around 15% of breast cancers are triple-negative breast cancer (TNBC), which is characterized by the absence of three common receptors—ER, PR, and HER2, and therefore do not respond to hormonal or anti-HER2 therapies. It is urgent to explore targeted therapeutic strategies for TNBC due to its poor prognosis and rare effective targeted therapy. In this study, we developed a polyamidoamine (PAMAM) dendrimer-based targeted gene delivery system—GDP-uPA, to utilize urokinase-type plasminogen activator (uPA) to target uPA receptor (uPAR), which is highly expressed in both TNBC cells and cancer-associated stromal cells. Our results of 1H NMR spectrum, TEM imaging, and MTT assay …

Protein Turnover Rate And Life History As An Explanation Of The Extreme Difference In Cost Of Biosynthesis In Cockroach Nymphs And Painted Lady Butterfly Larva, Kyara Nicole Holloway

Masters Theses

The cost of biosynthesis in painted lady caterpillars is far lower than that of a red runner cockroach. A promising explanation for this phenomenon is the protein turnover rate for the animal during biosynthesis and tissue maintenance. Previous research has shown that, per gram of body weight, the painted lady butterfly can synthesize a biomass using up to 15x less energy and resources than the cockroach, even under similar conditions. The resulting ratios, especially in respect to growth rate, show a wide disparity between species in such a manner that does support a protein synthesis and degradation rates as a …

2024 Recipient's Application, Kasey Christopher

Open Education Teaching Award

No abstract provided.

Fillet Weight Comparison For Bluegill, Black Crappie, And Yellow Perch, Mikenna A. Pattrin

Journal of Earth and Life Science

Panfish, including bluegill Lepomis macrochirus, black crappie Pomoxis nigromaculatus, and yellow perch Perca flavescens are three sought-after species. Anglers often perceive a change in regulations as an attempt at limiting the amount of harvest. However, the goal of many regulation proposals is to increase the size structure of panfish, allowing anglers to achieve their ideal harvest with less fish. The primary objective of this study was to assess the relationship between panfish fillet weight and total length to estimate how many fillets would be needed to reach a harvest weight of 0.5 lbs. The secondary objective was to …

Specific Measurements Of A Yellow Perch Head Compared To The Total Length, Alex Timm

Journal of Earth and Life Science

Yellow Perch Perca flavescens is a popular fish for harvesting in Minnesota. Because of this the Minnesota Department of Natural Resources survey populations with gill nets, however, some water bodies have Rusty Crayfish Faxonius rusticus that commonly consume most of the body leaving only the head to be accurately measured. The goal of this research was to see if there is a relationship between specific measurements of the head and the total length of the fish. The number of Yellow Perch sampled in this study was 72 and all came from Lake Bemidji in Minnesota during the month of October …

The Effects Of Early Exposure To Prescription/Illicit Drugs And Drug Metabolites On Larval Zebrafish Behavioral And Motor Function Development, Jacob Thomas, Dena Weinberger, Bikram Subedi, Brandon Capps

Murray State Theses and Dissertations

THE EFFECTS OF EARLY EXPOSURE TO PRESCRIPTION/ILLICIT DRUGS AND DRUG METABOLITES ON LARVAL ZEBRAFISH BEHAVIORAL AND MOTOR FUNCTION DEVELOPMENT

A Thesis Presented to the Faculty of the Department of Biology

Murray State University

Murray, Kentucky

In Partial Fulfillment of the Requirements for the Degree of Masters In Science

By Jacob Thomas, B.S., Murray State University

December 2024

Acknowledgements

Brandon Capps, who pretty much did a whole third of the legwork on this project and saved me lots of headaches;

Chandler Maddox, one of the University of Kentucky’s finest undergrads who came in from the top rope with a folding chair …

Using Habitat Modeling To Locate New Populations Of Dunn's Salamander (Plethodon Dunni) Near Its Southern Geographic Range Limit, Mary A. Mackey

Cal Poly Humboldt theses and projects

Habitat suitability models were utilized to direct survey efforts for Plethodon dunni in Northern California. Two models were created, one using traditional statistical methods (GLM or Generalized Linear Modeling) and the other using a machine learning method (MaxEnt or Maximum Entropy modeling). Plots were selected from areas of agreement and disagreement between the models to carry out presence surveys. The MaxEnt model yielded better predictions than the GLM model. The two models had three variables in common (annual temperature range, precipitation of driest month, and precipitation seasonality) and predicted suitable habitat within the known range of P. dunni. The …

Characterizing The Habitat Use Of Pacific Coast Feeding Group Gray Whales (Eschrichtius Robustus) And The Spatial And Temporal Variability Of Their Benthic And Planktonic Invertebrate Prey In Northern California, Robyn E. Norman

Cal Poly Humboldt theses and projects

As opportunistic foragers, the Eastern North Pacific population (~20,000) of gray whales (Eschrichtius robustus) feed on diverse benthic and planktonic invertebrates in northern Alaska foraging grounds before they undertake one of the largest yearly migrations of any mammal to breed in Baja California, Mexico. While most of the population travels to the summer foraging grounds in Alaska, a sub-group of whales (~230) called the Pacific Coast Feeding Group (PCFG) summer between British Columbia, Canada, and northern California. The diet of PCFG whales typically includes high-density and/or high-caloric prey items like mysids and diverse species of amphipods, yet a …

Using Biological Parameters And Traditional Ecological Knowledge To Study The Status Of Spirinchus Starksi (Night Smelt) In Northern California, Z. Zenobia

Cal Poly Humboldt theses and projects

Night smelt spawns from central California to southeast Alaska, on the shore of sandy beach surf zones at night. This species is a vital part of regional food webs and, in California, is fished commercially primarily in Humboldt and Del Norte counties. It has cultural importance for dietary and subsistence needs to the Tribes across the coast, as well as inland. The objectives of this thesis are as follow: study the status of the population of night smelt in Humboldt and Del Norte counties by (A) comparing the abundance, length, weight, age, sex ratio, and timing of reproduction of the …

Assessing The Effects Of Remaining Northern California Kelp Forest Mesopredators On Purple Sea Urchin Abundance, Distribution, And Feeding Activity, Sandra E. Traverso

Cal Poly Humboldt theses and projects

Kelp forests are threatened by regional abiotic and biotic stressors, including overgrazing by sea urchins. On the north coast of California, purple sea urchins (Strongylocentrotus purpuratus) are prolific grazers of kelp and with the regional extinction of their main predator (sunflower stars, Pycnopodia helianthoides), it is unknown whether any remaining mesopredators could exert top-down control of purple urchin populations. Other known predators of purple urchins in northern California include rock crabs, which have been shown to control sea urchin populations in Atlantic urchin barrens and indirectly cause phase shifts back to kelp forests. Yet, little is known …

Influence Of Seeding Strategy On Insect Communities Within Utility Lands, Kylee E. Nissen

Masters Theses

Pollinator populations are in decline globally due to anthropogenic changes, particularly in areas affected by agricultural expansion and urbanization. Utility lands - managed to prevent tall vegetation encroachment - represent an opportunity to establish native grasslands and support pollinator communities. This study investigates how different seeding strategies used by utility companies influence pollinator community composition within a 15-acre electric right-of-way (ROW) outside St. Louis, Illinois. Despite the logical benefits of native seeding to pollinators, utility companies are often hesitant to do this, often favoring seeding to turf. Seeded in late 2021, the site incorporates randomized plots with turfgrass seeding (no …

Nocturnal Behavior Of Communally Roosting American Crows (Corvus Brachyrhynchos), Caitlin M. Mrowiec

Masters Theses

During the winter months in Charleston, Illinois, thousands of American crows (Corvus brachyrhynchos) gather together each night to roost. While aspects of this impressive sight have been studied elsewhere, there is very limited information on how crows behave at night. Additionally, the mechanics and origins of communal roosting are not especially well understood, and the behaviors involved are not always consistently described. This study sought to provide a more complete assessment of crow communal roosting after sunset. I collected data between 2018 and 2020 by locating communal roosts and conducting continuous observations for 4 hours after sunset, with additional checks …

Diving At High Altitude: O2 Transport And Utilization In The Ruddy Duck And Torrent Duck In The Andes, Kevin G. Mccracken, Graham R. Scott, Luis Alza, Andrea Astie, Ciska Bakkeren, Emil Bautista, Mariana Bulgarella, Rebecca Cheek G. Cheek, Beverly A. Chua, Neal J. Dawson, Alexis Diaz, Catherine M. Ivy, Peter B. Frappell, Cecilia Kopuchian, Sabine L. Laguë, John N. Maina, Violeta Muñoz-Fuentes, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Thomas Valqui, Roy E. Weber, Daniela Wilner, Robert E. Wilson, Julia M. York, William K. Milsom

Occasional Papers of the Museum of Natural Science, Louisiana State University

Hypoxia and cold temperatures create unique physiological challenges for high-altitude organisms that can vary depending on lifestyle. While nearly all studies of air-breathing animals at high altitude are from terrestrial species, species that breath-hold dive underwater at high altitude encounter a very different set of selective pressures influencing their phenotype. The goal of this publication is to highlight the changes in O2 transport and utilization in high-altitude diving birds relative to divers at sea level, and the extent to which these changes are qualitatively distinct from phenotypic changes in non-diving species at high altitude. For example, while high capacities for …

Development Of Innovative Flocculation Technologies For Agricultural Water Treatment, Noor Haleem

Electronic Theses and Dissertations

The development of innovative flocculation technologies is essential for addressing the challenges of agricultural water treatment. These technologies play a crucial role in removing contaminants such as suspended solids and nutrients, thereby ensuring safer water for irrigation and livestock consumption. By enhancing water quality and wastewater management, they contribute significantly to environmental sustainability and public health in agricultural communities. This comprehensive thesis extensively explores various dimensions of flocculation, with a focused effort on methodologies and resources aimed at strengthening sustainability and efficiency. A pivotal aspect of the research involves synthesizing cationic starch (CS), a flocculant derived from an underutilized resource, …

Considerations And Applications Of Environmental Dna In Biodiversity Studies, Sarah Tomke

Theses and Dissertations--Forestry and Natural Resources

Environmental DNA (eDNA) sampling—the detection of species through genetic material shed into the environment—has become an efficient and reliable method for biodiversity assessments. The method has grown in popularity due to its non-invasive and non-destructive nature, and has revolutionized monitoring efforts for rare, elusive, and cryptic species for which traditional sampling approaches are often costly and ineffective. Hundreds of studies have utilized this technique to study species occupancy, delineate distributions, and assess relative community composition. Although eDNA sampling is an effective strategy for many conservation and ecological studies, recently, light has been shed on some of its potential drawbacks and …

Behavioral Ecology Of The Colorado Brown Tarantula (Aphonopelma Hentzi): Activities Displayed Around The Burrow Through Juvenile Group Dispersal, Bradley Allendorfer

Master's Theses and Doctoral Dissertations

The life history strategy of the Colorado brown tarantula (Aphonopelma hentzi) typically includes males spatially scattering in search for a female mate in the fall, followed by female attendance of fertilized eggs the following summer. Here, I highlighted aspects associated with this female parental care. Specifically, I compared the activities of maternal (egg-attending) tarantulas to their non-maternal (not egg-attending) counterparts, as this may be a costly time for these egg-attending individuals. I concluded that maternal individuals tended to show reduced activity at the burrow entrance, however; these, results were significant in only one comparison. After weeks of this parental care, …

Male Beetle Courtship Dance Analysis By Deeplabcut Technology, Andrea Newbrough

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Assessing The Feasibility Of Seaweed Farm-Offshore Wind Co-Location In New Jersey, Brianna Delaney Reynolds

Theses, Dissertations and Culminating Projects

New Jersey’s offshore wind energy sector may provide an opportunity to advance the seaweed farming industry through ocean multi-use. Yet, opportunities, constraints, and knowledge gaps for seaweed farm-offshore wind co-location remain largely uninvestigated. Here, I determine the suitability of native seaweed species for offshore cultivation in New Jersey and conduct a quick scoping review (QSR) to assess the knowledge base surrounding seaweed farm-offshore wind multi-use. Saccharina latissima and Fucus vesiculosus were identified as native species with commercial value for cultivation in New Jersey. Yet, offshore waters at the location of a wind development site had insufficient nutrients to meet the …