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Inferring And Testing Hypotheses Of Cladistic Character Dependence By Using Character Compatibility, F. Robin O’Keefe, Peter J. Wagner Sep 2001

Inferring And Testing Hypotheses Of Cladistic Character Dependence By Using Character Compatibility, F. Robin O’Keefe, Peter J. Wagner

Biological Sciences Faculty Research

The notion that two characters evolve independently is of interest for two reasons. First, theories of biological integration often predict that change in one character requires complementary change in another. Second, character independence is a basic assumption of most phylogenetic inference methods, and dependent characters might confound attempts at phylogenetic inference. Previously proposed tests of correlated character evolution require a model phylogeny and therefore assume that nonphylogenetic correlation has a negligible effect on initial tree construction. This paper develops “tree-free” methods for testing the independence of cladistic characters. These methods can test the character independence model as a hypothesis before …


Genomes, Gould, And Emergence, Ursula Goodenough Sep 2001

Genomes, Gould, And Emergence, Ursula Goodenough

Biology Faculty Research

The publication of the human genome has elicited commentary to the effect that, since fewer genes were identified than anticipated, it follows that genes are less important to human biology than anticipated. The flaws in this syllogism are explained in the context of a treatise on how genomes operate and evolve and how genes function to produce embryos and brains. Most of our most cherished human traits are the result of the emergence of new properties from preexisting genetically scripted ideas, offering countless opportunities to celebrate the evolutionary process.


Synaptic Heterogeneity And Stimulus-Induced Modulation Of Depression In Central Synapses, John D. Hunter, John Milton Aug 2001

Synaptic Heterogeneity And Stimulus-Induced Modulation Of Depression In Central Synapses, John D. Hunter, John Milton

WM Keck Science Faculty Papers

Short-term plasticity is a pervasive feature of synapses. Synapses exhibit many forms of plasticity operating over a range of time scales. We develop an optimization method that allows rapid characterization of synapses with multiple time scales of facilitation and depression. Investigation of paired neurons that are postsynaptic to the same identified interneuron in the buccal ganglion of Aplysia reveals that the responses of the two neurons differ in the magnitude of synaptic depression. Also, for single neurons, prolonged stimulation of the presynaptic neuron causes stimulus-induced increases in the early phase of synaptic depression. These observations can be described by a …


Puerto Rican Karst - A Vital Resource, Ariel E. Lugo, Leopoldo Miranda Castro, Abel Vale, Tania Del Mar López, Enrique Hernández Prieto, Andrés García Martinó, Alberto R. Puente Rolón, Adrianne G. Tossas, Donald A. Mcfarlane, Tom Miller, Armando Rodríguez, Joyce Lundberg, John Thomlinson, José Colón, Johannes H. Schellekens, Olga Ramos, Eileen Helmer Aug 2001

Puerto Rican Karst - A Vital Resource, Ariel E. Lugo, Leopoldo Miranda Castro, Abel Vale, Tania Del Mar López, Enrique Hernández Prieto, Andrés García Martinó, Alberto R. Puente Rolón, Adrianne G. Tossas, Donald A. Mcfarlane, Tom Miller, Armando Rodríguez, Joyce Lundberg, John Thomlinson, José Colón, Johannes H. Schellekens, Olga Ramos, Eileen Helmer

WM Keck Science Faculty Papers

The limestone region of Puerto Rico covers about 27.5 percent of the island's surface and is subdivided into the northern, southern, and dispersed limestone areas. All limestone areas have karst features. The karst belt is that part of the northern limestone with the most spectacular surficial karst landforms. It covers 142,544 ha or 65 percent of the northern limestone. The karst belt is the focus of this publication, although reference is made to all limestone regions. The northern limestone contains Puerto Rico's most extensive freshwater aquifer, largest continuous expanse of mature forest, and largest coastal wetland, estuary, and underground cave …


Transduction By Phi Bb-1, A Bacteriophage Of Borrelia Burgdorferi, Christian H. Eggers, Betsy J. Kimmel, James L. Bono, Abdallah F. Elias, Patricia Rosa, D. Scott Samuels Aug 2001

Transduction By Phi Bb-1, A Bacteriophage Of Borrelia Burgdorferi, Christian H. Eggers, Betsy J. Kimmel, James L. Bono, Abdallah F. Elias, Patricia Rosa, D. Scott Samuels

Biological Sciences Faculty Publications

We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phi BB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phi BB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phi BB-1 released from this B. …


A Heat-Shock-Activated Cdna Encoding Gaga Factor Rescues Some Lethal Mutations In The Drosophila Melanogaster Trithorax-Like Gene, H Granok, B Leibovitch, Sarah C.R. Elgin Aug 2001

A Heat-Shock-Activated Cdna Encoding Gaga Factor Rescues Some Lethal Mutations In The Drosophila Melanogaster Trithorax-Like Gene, H Granok, B Leibovitch, Sarah C.R. Elgin

Biology Faculty Research

GAGA factor is an important chromosomal protein involved in establishing specific nucleosome arrays and in regulating gene transcription in Drosophila melanogaster. We developed a transgenic system for controlled heat-shock-dependent overexpression of the GAGA factor 519 amino acid isoform (GAGA-519) in vivo. Efficient production of stable protein from these transgenes provided genetic rescue of a hypomorphic Trithorax-like (Trl) lethal allele to adulthood. Nevertheless, supplemental GAGA-519 did not suppress position effect variegation (PEV), a phenomenon commonly used to measure dosage effects of chromosomal proteins, nor did it rescue other lethal alleles of Trl. The results suggest requirements for the additional isoforms of …


The Impact Of Logging On Aquatic Salamander Communities., Heather Noel Mcdonald Aug 2001

The Impact Of Logging On Aquatic Salamander Communities., Heather Noel Mcdonald

Electronic Theses and Dissertations

For this study, the abundance of the salamander fauna in two streams was compared to determine the impact of logging activities. After five months of study, the area around one stream was logged by the National Forest Service. Following logging, the abundance of salamanders in the stream was greatly reduced, while the population in the undisturbed stream remained relatively unchanged. Six abundant species were used as indicators; two of these species (Desmognathus quadramaculatus and Desmognathus ochrophaeus) were negatively affected by the logging, while four species (Demognathus fuscus, Desmognathus monticola, Eurycea wilderae, and Gyrinophilus porphyriticus …


Development And Characterization Of Monoclonal Antibodies Directed Against Yaba-Like Disease Virus, Scott Haller Aug 2001

Development And Characterization Of Monoclonal Antibodies Directed Against Yaba-Like Disease Virus, Scott Haller

Masters Theses

The hybridoma technique has been used for the production of monoclonal antibodies (mAb) directed against yaba-like disease virus (YLDV). Spleen cells, obtained from female BALB/c mice immunized with YLDV, were fused with P3x63.Ag8.653 myeloma cells and hybrids were selected. Hybridoma clones were screened for production of anti-YLDV antibodies by Indirect Immunofluorescence (IF) microscopy. Positive hybridoma clones were recloned via limiting dilution and characterized. A single clone (H12) has been selected that specifically reacted with YLDV infected cells and not uninfected cells. Immunodiffusion technique determined H12 monoclonal antibody as an IgG1 isoptype immunoglobulin. H12 antibodies recognized a late YLDV protein, and …


Avian And Mammalian Use Of Three Small Urban Wetlands Invaded By A Pest Species, Reed Canarygrass (Phalaris Arundinacea L.), Laurie Dizney Jul 2001

Avian And Mammalian Use Of Three Small Urban Wetlands Invaded By A Pest Species, Reed Canarygrass (Phalaris Arundinacea L.), Laurie Dizney

Dissertations and Theses

Small urban wetlands are being destroyed and degraded at an alarming rate. One result of degradation is invasion by pest species, such as reed canarygrass (Phalaris arundinacea L. ). Reed canarygrass forms virtual monocultures in a wide range of wetland types. Wetlands normally have high plant species richness and abundance, which is widely believed to lead to faunal diversity. The purpose of the present study was to determine to what extent birds and mammals use a wetland dominated by reed canarygrass. The research was conducted at Tualatin Hills Nature Park in Beaverton, Washington County, Oregon. I examined three sites …


Temperature Effects On The Dynamics Of Aedes Albopictus (Diptera : Culicidae) Populations In The Laboratory, Barry W. Alto, Steven A. Juliano Jul 2001

Temperature Effects On The Dynamics Of Aedes Albopictus (Diptera : Culicidae) Populations In The Laboratory, Barry W. Alto, Steven A. Juliano

Faculty Publications – Biological Sciences

We investigated how constant temperatures of 22, 24, and 26 degreesC experienced across the full life cycle affected the dynamics of caged populations of Aedes albopictus (Skuse). All cages were equipped with plastic beakers that served as sites for oviposition and larval development. We measured the per capita daily mortality and emergence rates of the adults and size of adult females, and estimated the intrinsic rate of increase (r) and asymptotic density (K) for each adult population. populations at 26 degreesC had greater intrinsic rates of increase and lower asymptotic densities than populations at 22 and 24 degreesC. Populations at …


Molecular Diversity, Structure And Domestication Of Grasses, Edward S. Buckler Iv, Jeffry M. Thornsberry, Stephen Kresovich Jun 2001

Molecular Diversity, Structure And Domestication Of Grasses, Edward S. Buckler Iv, Jeffry M. Thornsberry, Stephen Kresovich

Faculty Publications

Over the last 10,000 years, crop domestication has been the single most important human cultural development. Grasses are prominent among these crops, and provide the vast majority of the world's food. Similar traits have been selected during the domestication and breeding of these critically important grasses, and since they share a similar complement of genes, the same set of genes may have been selected. Even though the process of domestication occurred over the same 5000 to 10,000 year period, the domesticated grasses have major differences in genome structure, diversity, and life history. Molecular investigations of grass domestication have succeeded in …


Parasitism Patterns And The Size-Fecundity Relationship In The Acoustically Orienting Dipteran Parasitoid Ormia Ochracea, Gita R. Kolluru, Marlene Zuk Jun 2001

Parasitism Patterns And The Size-Fecundity Relationship In The Acoustically Orienting Dipteran Parasitoid Ormia Ochracea, Gita R. Kolluru, Marlene Zuk

Biological Sciences

Female parasitoids are expected to distribute offspring among hosts in a manner that maximizes fitness. Several theoretical and empirical studies have focussed on clutch-size patterns in hymenopteran parasitoids. In contrast, dipteran parasitoids, which differ from hymenopterans in potentially important ways, have received little attention. The phonotactic tachinid fly Ormia ochracea has been intensively studied for its effects on host crickets, and has recently been the subject of studies of its own reproductive biology. This work suggests a negative relationship between clutch size and progeny fitness (consistent with hymenopterans), but no adjustment of clutch size to host size (different from hymenopterans). …


Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric K. Bollinger Jun 2001

Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric K. Bollinger

Faculty Research & Creative Activity

This book review appeared in The Auk, Volume 118 No. 3 (July 2001).


Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric Bollinger Jun 2001

Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric Bollinger

Faculty Research & Creative Activity

This book review appeared in The Auk, Volume 118 No. 3 (July 2001).


Invasion Genetics Of New World Medflies: Testing Alternative Colonization Scenarios, Andrew J. Bohonak, Neil Davies, Francis X. Villablanca, George K. Roderick Jun 2001

Invasion Genetics Of New World Medflies: Testing Alternative Colonization Scenarios, Andrew J. Bohonak, Neil Davies, Francis X. Villablanca, George K. Roderick

Biological Sciences

The Mediterranean fruit fly (Ceratitis capitata) is an invasive agricultural pest with a wide host range and a nearly global distribution. Efforts to forgo the medfly''s spread into the United States are dependent on an understanding of population dynamics in newly established populations elsewhere. To explore the potential influence of demographic and historical parameters in six medfly populations distributed from Mexico to Peru, we created population genetic null models using Monte Carlo simulations. Null expectations for genetic differentiation (F ST) were compared with actual sequence variation from four highly polymorphic nuclear loci. Four colonization scenarios that …


The Mayfly Newsletter, Peter M. Grant Jun 2001

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.


High Species Diversity In Fleshy-Fruited Tropical Understory Plants, James F. Smith Jun 2001

High Species Diversity In Fleshy-Fruited Tropical Understory Plants, James F. Smith

Biology Faculty Publications and Presentations

Key innovations may increase the number of taxa in a clade that possesses the proposed innovation in comparison to its sister group that lacks the trait through either increased speciation or reduced extinction rates. Comparing sister clades across several independent lineages provides statistical support that the trait has increased species diversity. Previous studies have indicated that there may not be a relationship between biotic dispersal and higher species diversity, but only a few of these studies specified habit, habitat, or type of disperser. No previous study has specified all of the above parameters and used a phylogenetic approach. This article …


A Setback To The Dialogue: Response To Huston Smith, Ursula Goodenough Jun 2001

A Setback To The Dialogue: Response To Huston Smith, Ursula Goodenough

Biology Faculty Research

Huston Smith's book, Why Religion Matters, offers an eloquent evocation of mystical sensibility. Unfortunately, along the way, he offers a strongly negative and often inaccurate account of the scientific worldview, the claim being that the science is laying siege to the spiritual.


Functional Properties Of Slow And Fast Gastrocnemius Muscle Fibers After A 17-Day Spaceflight, Jeffrey J. Widrick, Janell Romatowski, Kris M. Norenberg, Shannon T. Knuth, James L. W. Bain, Danny A. Riley, S. W. Trappe, Todd A. Trappe, David Costill, Robert H. Fitts Jun 2001

Functional Properties Of Slow And Fast Gastrocnemius Muscle Fibers After A 17-Day Spaceflight, Jeffrey J. Widrick, Janell Romatowski, Kris M. Norenberg, Shannon T. Knuth, James L. W. Bain, Danny A. Riley, S. W. Trappe, Todd A. Trappe, David Costill, Robert H. Fitts

Biological Sciences Faculty Research and Publications

The purpose of this investigation was to study the effects of a 17-day spaceflight on the contractile properties of individual fast- and slowtwitch fibers isolated from biopsies of the fast-twitch gastrocnemius muscle of four male astronauts. Single chemically skinned fibers were studied during maximal Ca2+-activated contractions with fiber myosin heavy chain (MHC) isoform expression subsequently determined by SDS gel electrophoresis. Spaceflight had no significant effect on the mean diameter or specific force of single fibers expressing type I, IIa, or IIa/IIx MHC, although a small reduction in average absolute force (Po) was observed for the type …


The Wings Of Bombyx Mori Develop From Larval Discs Exhibiting An Early Differentiated State: A Preliminary Report, Madhuri Kango-Singh, Amit Singh, K. P. Gopinathan Jun 2001

The Wings Of Bombyx Mori Develop From Larval Discs Exhibiting An Early Differentiated State: A Preliminary Report, Madhuri Kango-Singh, Amit Singh, K. P. Gopinathan

Biology Faculty Publications

Lepidopteran insects present a complex organization of appendages which develop by various mechanisms. In the mulberry silkworm, Bombyx mori, a pair of meso- and meta-thoracic discs located on either side in the larvae gives rise to the corresponding fore- and hind-wings of the adult. These discs do not experience massive cell rearrangements during metamorphosis and display the adult wing vein pattern. We have analysed wing development in B. mori by two approaches, viz., expression of patterning genes in larval wing discs, and regulatory capacities of larval discs following explantation or perturbation. Expression of Nubbin is seen all over the presumptive …


Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto Jun 2001

Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto

Veterinary Science Faculty Publications

In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …


Salivary Acinar Cells From Aquaporin 5-Deficient Mice Have Decreased Membrane Water Permeability And Altered Cell Volume Regulation, Carissa M. Krane, James E. Melvin, Ha-Van Nguyen, Linda Richardson, Jennifer E. Towne, Thomas Doetschman, Anil G. Menon Jun 2001

Salivary Acinar Cells From Aquaporin 5-Deficient Mice Have Decreased Membrane Water Permeability And Altered Cell Volume Regulation, Carissa M. Krane, James E. Melvin, Ha-Van Nguyen, Linda Richardson, Jennifer E. Towne, Thomas Doetschman, Anil G. Menon

Biology Faculty Publications

Aquaporins (AQPs) are channel proteins that regulate the movement of water through the plasma membrane of secretory and absorptive cells in response to osmotic gradients. In the salivary gland, AQP5 is the major aquaporin expressed on the apical membrane of acinar cells. Previous studies have shown that the volume of saliva secreted by AQP5-deficient mice is decreased, indicating a role for AQP5 in saliva secretion; however, the mechanism by which AQP5 regulates water transport in salivary acinar cells remains to be determined. Here we show that the decreased salivary flow rate and increased tonicity of the saliva secreted byAqp5 …


Ecology Of Audubon's Shearwaters (Puffinus Lherminieri) At San Salvador, Bahamas, Neville Anthony Trimm Jr. Jun 2001

Ecology Of Audubon's Shearwaters (Puffinus Lherminieri) At San Salvador, Bahamas, Neville Anthony Trimm Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

The Audubon's Shearwater (Pqffinus lherminieri), nearly pantropical in distribution, remains a poorly studied species. The subspecies P. l. lherminieri is endemic to the West Indies region. An estimated 5,000 pairs inhabit the Caribbean, but there has been a substantial decline with many colonies becoming extirpated. It is thought that as many as 3,000 pairs (60% of the West Indian population) nest in the Bahamas. In May and June of 2000, I collected data on shearwater demographics, colony site selection, burrow microhabitat, chick behavior and the effect of moonlight on the onset of calling of the Audubon's Shearwaters nesting on …


Ecology Of The Endangered Sandy Cay Rock Iguana, Cyclura Rileyi Cristata, In The Bahamas, Shawn Kendall Fry Jun 2001

Ecology Of The Endangered Sandy Cay Rock Iguana, Cyclura Rileyi Cristata, In The Bahamas, Shawn Kendall Fry

Loma Linda University Electronic Theses, Dissertations & Projects

In this thesis I present the results of a study on the population demographics and home range size of Cyclura rileyi cristata. There are eight species of West Indian rock iguanas, genus Cyclura. All are endangered, including C. rileyiwhich is endemic to the Bahamas. One of three recognized subspecies, C. r. cristatalives on a single cay in the Exumas chain and is among the most endangered lizards in the world. Various size measurements indicate that this taxon is among the smallest within the genus. Distance surveys and a Lincoln-Petersen estimate based on resightings of marked iguanas …


First Nesting Records Of The Black Phoebe (Sayornis Nigricans) In Southernmost Texas, Timothy Brush Jun 2001

First Nesting Records Of The Black Phoebe (Sayornis Nigricans) In Southernmost Texas, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

No abstract provided.


Effect Of Forest Age On Density Of Stream-Breeding Amphibians In Skamania County, Washington, Craig A. Steele May 2001

Effect Of Forest Age On Density Of Stream-Breeding Amphibians In Skamania County, Washington, Craig A. Steele

Biology

Because the ranges of stream-breeding amphibians in the Pacific Northwest occur partly on industrial timberlands, it is valuable to study population distributions of these animals in forest environments that rarely reach old growth status and are subject to timber harvest on a regular basis. To determine how short-term changes in forest age affect the density of stream-breeding amphibians, first-order streams located on private industrial timberlands in Skamania County, Washington, were sampled during the summers of 1998 and 1999. Species captured included Dicamptodon tenebrosus, Dicamptodon copei, Rhyacotriton cascadae, and Ascaphus truei. Regression tree analysis showed that relative …


Switching Between Intensive And Extensive Searching In Predators Of Heterogeneously Distributed Prey, Zy Biesinger May 2001

Switching Between Intensive And Extensive Searching In Predators Of Heterogeneously Distributed Prey, Zy Biesinger

Biology

Many predators of patchily distributed prey are thought to increase their searching efficiency by alternating between extensive and intensive searching behavior. To test the effects of predator satiation and prey density on a ladybird's choice of searching mode, I performed experiments in which larvae of different hunger levels experienced different prey encounter rates. Using traditional measures, I found a beetle's satiation level to have no effect. The prey encounter rate had an effect on two of three traditional measures. Less traditional methods, fractal dimension analysis and Fourier slope analysis, supported the conclusions obtained using traditional measures. Analysis of movement patterns …


Assemblages Of Spiders On Shrubs In Semiarid Environments: Experimental Models Of Shrub Architecture, Michael W. Heikkinen May 2001

Assemblages Of Spiders On Shrubs In Semiarid Environments: Experimental Models Of Shrub Architecture, Michael W. Heikkinen

Biology

Assemblages of spiders on big sagebrush are dominated by salticids. The pattern could result from many salticids in the colonist pool and random distribution as spiders disperse or a selection process by which spiders are choosing to remain on big sagebrush after initial colonization. Choices may be based on insect prey on the shrubs or related to the plant's physical attributes. Habitat structure has been associated with the distribution of many animals, and plant architecture influences the distribution of spiders. Models of big sagebrush were constructed to test plant architecture variables that spiders may use as cues in deciding to …


Phylogenetic Relationships, Taxonomy, And Evolution Of Host Choice In Diadasia (Hymenoptera: Apoidea), Sedonia D. Sipes May 2001

Phylogenetic Relationships, Taxonomy, And Evolution Of Host Choice In Diadasia (Hymenoptera: Apoidea), Sedonia D. Sipes

Biology

I examined the phylogenetic relationships, taxonomy, and evolution of host choice in Diadasia, a genus of New World bees. Diadasia species are particularly interesting because they are considered pollen specialists. Females of each species are believed to provision their nests with pollen from only a few closely related plant taxa. At the same time, the choice of floral hosts within the genus is diverse, and includes five distantly related plant families: Malvaceae, Onagraceae, Convolvulaceae, Asteraceae, and Cactaceae. To estimate phylogenetic relationships within this group, I sequenced ~ 2 kb of the mitochondrial genes CO I and II and tRNA …


Diversity, Distribution, And Abundance Of Flower-Visiting Apoidea (Hymenoptera) On Sand Dunes Of The West Desert Of Utah, Trent R. Toler May 2001

Diversity, Distribution, And Abundance Of Flower-Visiting Apoidea (Hymenoptera) On Sand Dunes Of The West Desert Of Utah, Trent R. Toler

Biology

Three habitats (dunes, mountain slopes, and mud flats) were surveyed for bees (Hymenoptera: Apoidea) in the Bonneville Basin in Utah on lands owned by the U.S. military, the Dugway Proving Grounds and the southern section of the Utah Testing and Training Range, in the summers of 1997 and 1998. Study of dune habitat was emphasized, as it is thought to be important for bees in arid climates, particularly in providing nesting substrate and sources of plant pollen and nectar. Species compositions of bees were determined using pan traps at 16 sites (in three habitats) in 1997, and at 5 dune …