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Variations In The Histamine Content Of Mink Testes Due To Injections Of Gonadotrophic Hormones And Application Of Free Radicals In Vitro, Grant I. Wilson

Biology

It was hypothesized that gonadotropic hormones and oxygen-derived free radicals affect histamine production in the testes. Luteinizing hormone, follicle stimulating hormone, prolactin, a combination of these three hormones, or saline (controls) were injected for either 4 or 7 days into seven wild-type (demi) and seven dark male mink per group. All mink were euthanized with carbon monoxide. Their testes were removed and frozen on dry ice. The histamine content was analyzed using radioimmunoassay. The control wild-type mink injected for either 4 or 7 days had histamine values of 8.15 μg/g and 7.56 μg/g, respectively. In wild-type mink, there was no …

Natural History And Roosting Ecology Of The Eastern Pipistrelle, Pipistrellus Subflavus, Jacques Pierre Veilleux

All-Inclusive List of Electronic Theses and Dissertations

A study concerning the natural history and roosting ecology of the eastern pipistrelle, Pipistrellus subtlavus, was conducted from 1997 to 2000, in southern Vigo County, Indiana. 111 Females began forming pre-maternity colonies during early May, and parturition began during late June. Pre-maternity colonies averaged 3.7 ± 1.9 individuals, and maternity colonies averaged 4.4 ± 2.4 individuals. Young pipistrelles first became volant during the third and fourth weeks of July. Pipistrelles were located in 59 roost trees. All roosts were located in foliage, either in clusters of dead leaves (n = 40) or in live foliage (n = 19). Oaks (Ouercus …

Trophic Cascades, Nutrients, And Lake Productivity: Whole-Lake Experiments, Stephen R. Carpenter, Jonathan J. Cole, James R. Hodgson, James F. Kitchell, Michael L. Pace, Darren Bade, Kathryn L. Cottingham

Dartmouth Scholarship

Responses of zooplankton, pelagic primary producers, planktonic bacteria, and CO2 exchange with the atmosphere were measured in four lakes with contrasting food webs under a range of nutrient enrichments during a seven-year period. Prior to enrichment, food webs were manipulated to create contrasts between piscivore dominance and planktivore dominance. Nutrient enrichments of inorganic nitrogen and phosphorus exhibited ratios of N:P > 17:1, by atoms, to maintain P limitation. An unmanipulated reference lake, Paul Lake, revealed baseline variability but showed no trends that could confound the interpretation of changes in the nearby manipulated lakes. Herbivorous zooplankton of West Long Lake (piscivorous fishes) …

Lipid Biochemistry Of House Wren Egg Yolk And Possible Effects On Incubation Period, Brian J. Payne '01

Honors Projects

House Wrens (Troglodytes aedon) are distributed throughout North and South America. As has been observed in many avian species, populations of House Wrens in the tropics have longer egg incubation periods than populations in temperate regions. The purpose of this study was to characterize incubation periods of a temperate population of House Wrens and to conduct a preliminary chemical analysis of the yellow and white yolk layers of House Wren eggs. The eggs were collected from a breeding population in Central Illinois from June-August 2000. Avian yolk is composed of yellow and white components, which are laid down in alternating …

Bird Migration Through A Mountain Pass Studied With High Resolution Radar, Ceilometers, And Census, Timothy C. Williams , '64, J. M. Williams, P. G. Williams, P. Stokstad

Biology Faculty Works

Autumnal migration was studied with high-resolution radar, ceilometer, and daily census in the area of Franconia Notch, a major pass in the northern Appalachian Mountains. Under synoptic conditions favorable for migration, broadfront movements of migrants toward the south passed over the mountains, often above a temperature inversion. Birds at lower elevations appeared to be influenced by local topography. Birds moving southwest were concentrated along the face of the mountain range. Birds appeared to deviate their flights to follow local topography through the pass. Specific migratory behavior was not associated with species or species groups. Under synoptic conditions unfavorable for southward …

Determining Differences In The Spatial Distribution Of Forest Structure On The Kaibab Plateau: Implications For Forest Management And The Northern Goshawk, Ryan S. Miller

Other Publications in Wildlife Management

The Kaibab Plateau, in North Central Arizona, has undergone extensive change in the last 100 years due to land management practices such as logging, road building, and fire suppression. The northern goshawk (Accipiter gentilis) has been a center of controversy, due to the potential effects of silvicultural practices on goshawk breeding habitat (Reynolds-1983, Bloom et al 1986, Kennedy 1989, Crocker-Bedford 1990). Current and past research efforts on the Kaibab Plateau have mapped Goshawk nesting territories and temporal change in nesting behavior and success. However, these research efforts have not determined how long-term spatial changes in land-use activities that have influenced …

Treefall Gaps And The Maintenance Of Species Diversity In A Tropical Forest, Stefan A. Schnitzer, Walter P. Carson

Biological Sciences Faculty Research and Publications

The maintenance of species diversity by treefall gaps is a long‐standing paradigm in forest ecology. Gaps are presumed to provide an environment in which tree species of differing competitive abilities partition heterogeneous resources. The empirical evidence to support this paradigm, however, remains scarce, and some recent studies even suggest that gaps do not maintain the diversity of shade‐tolerant species. Although there is evidence that gaps maintain the diversity of pioneer trees, most of this evidence comes from studies that did not make comparisons between gaps and intact forest sites (controls). Further, nearly all studies on the maintenance of diversity by …

Environmental Effects On Yield And Agronomic Traits Of Common Bean (Phaseolus Vulgaris L.), Tadesse Mebrahtu, Teklu Andebrhan, Ali Mohamed

Virginia Journal of Science

Common bean (Phaseolus vulgaris L.) demand is increasing with an alarming rate around the world, especially in Latin America, Africa, and Asia. Therefore, increase bean yield per hectare is the best way to meet the world demand rather than expansion of area under cultivation. The objectives of this experiment were to determine the genotypic variations for green bean and dry seed yield and magnitude of genotype x environment interaction effects on yield and yield components of common bean. Thirteen genotypes were planted during the 1992, 1994, and 1995 growing seasons. Genotypes were evaluated for green pod and seed …

Binding Of Urate And Caffeine To Hemocyanin Analyzed By Isothermal Titration Calorimetry, Michael A. Menze, Nadja Hellmann, Heinz Decker, Manfred K. Grieshaber

Faculty Research & Creative Activity

Haemocyanin serves as an oxygen carrier in the haemolymph of decapod crustaceans. The oxygen-binding behaviour of the pigment is modulated by the two major anaerobic metabolites, L-lactate and urate. The binding of these two metabolites to haemocyanin has been investigated mainly indirectly by following the effectorinduced changes in the oxygen-binding properties of the respiratory pigment. Only a few direct investigations of effector binding, employing ultracentrifugation techniques and equilibrium dialysis, have been carried out. No evidence for cooperative binding for either effector was detected using these methods. However, isothermal titration calorimetry (ITC) offers a useful tool to gain additional insight into …

Experimental Model Of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella Quintana, Pierre-Edouard Fournier, Michael F. Minnick, Hubert Lepidi, Eric Salvo, Didier Raoult

Biological Sciences Faculty Publications

A laboratory colony of human body lice was experimentally infected by feeding on rabbits made artificially bacteremic with a green fluorescent protein-expressing Bartonella quintana. B. quintana was detected in the gut and feces until death but not in the eggs. The life span of the lice was not modified. The rabbit model should provide valuable clues to the role of lice in the transmission of B. quintana.

Vertical And Horizontal Transcendence, Ursula Goodenough

Biology Faculty Research

Transcendence is explored from two perspectives: the traditional concept wherein the origination of the sacred is “out there,” and the alternate concept wherein the sacred originates “here.” Each is evaluated from the perspectives of aesthetics and hierarchy. Both forms of transcendence are viewed as essential to the full religious life.

Contributions Of Identifiable Neurons And Neuron Classes To Lamprey Vertebrate Neurobiology, James T. Buchanan

Biological Sciences Faculty Research and Publications

Among the advantages offered by the lamprey brainstem and spinal cord for studies of the structure and function of the nervous system is the unique identifiability of several pairs of reticulospinal neurons in the brainstem. These neurons have been exploited in investigations of the patterns of sensory input to these cells and the patterns of their outputs to spinal neurons, but no doubt these cells could be used much more effectively in exploring their roles in descending control of the spinal cord. The variability of cell positions of neurons in the spinal cord has precluded the recognition of unique spinal …

Fiber-Type Susceptibility To Eccentric Contraction-Induced Damage Of Hindlimb-Unloaded Rat Al Muscles, K. P. Vijayan, Joyce L. Thompson, Kris M. Norenberg, Robert H. Fitts, Danny A. Riley

Biological Sciences Faculty Research and Publications

Slow oxidative (SO) fibers of the adductor longus (AL) were predominantly damaged during voluntary reloading of hindlimb unloaded (HU) rats and appeared explainable by preferential SO fiber recruitment. The present study assessed damage after eliminating the variable of voluntary recruitment by tetanically activating all fibers in situ through the motor nerve while applying eccentric (lengthening) or isometric contractions. Muscles were aldehyde fixed and resin embedded, and semithin sections were cut. Sarcomere lesions were quantified in toluidine blue-stained sections. Fibers were typed in serial sections immunostained with antifast myosin and antitotal myosin (which highlights slow fibers). Both isometric and eccentric paradigms …

Binding Of Urate And Caffeine To Hemocyanin Analyzed By Isothermal Titration Calorimetry., Michael Menze, Nadja Hellmann, Heinz Decker, Manfred Grieshaber

Faculty and Staff Scholarship

Haemocyanin serves as an oxygen carrier in the haemolymph of decapod crustaceans. The oxygen-binding behaviour of the pigment is modulated by the two major anaerobic metabolites, L-lactate and urate. The binding of these two metabolites to haemocyanin has been investigated mainly indirectly by following the effectorinduced changes in the oxygen-binding properties of the respiratory pigment. Only a few direct investigations of effector binding, employing ultracentrifugation techniques and equilibrium dialysis, have been carried out. No evidence for cooperative binding for either effector was detected using these methods. However, isothermal titration calorimetry (ITC) offers a useful tool to gain additional insight into …

Chromosome Movement In Mitosis Requires Microtubule Anchorage At Spindle Poles, Michael B. Gordon, Louisa Howard, Duane A. Compton

Dartmouth Scholarship

Anchorage of microtubule minus ends at spindle poles has been proposed to bear the load of poleward forces exerted by kinetochore-associated motors so that chromosomes move toward the poles rather than the poles toward the chromosomes. To test this hypothesis, we monitored chromosome movement during mitosis after perturbation of nuclear mitotic apparatus protein (NuMA) and the human homologue of the KIN C motor family (HSET), two noncentrosomal proteins involved in spindle pole organization in animal cells. Perturbation of NuMA alone disrupts spindle pole organization and delays anaphase onset, but does not alter the velocity of oscillatory chromosome movement in prometaphase. …

Sustainable Tourism Plan For The Houtman Abrolhos Islands, Fisheries Western Australia

Fisheries Management Papers

The Abrolhos coral reefs are the southernmost living reefs in the Indian Ocean. They have a unique assemblage of tropical species of fish, reef corals and other invertebrates, which live in close association with temperate species, particularly of algae, and species endemic to Western Australia. The Abrolhos is the centre of the fishery for the commercially valuable western rock lobster (Panulirus cygnus). Terrestrial flora and fauna of the Abrolhos are relict and provide important reference areas on ecological interactions. Virtually all the islands of the Abrolhos archipelago have sea bird nesting and breeding areas, and populations of some species of …

Post-Fledging Dispersal Of Burrowing Owls In Southwestern Idaho: Characterization Of Movements And Use Of Satellite Burrows, R. Andrew King, James R. Belthoff

Biology Faculty Publications and Presentations

Using radiotelemetry, we monitored dispersing juvenile Western Burrowing Owls (Athene cunicularia hypugaea) within a migratory population in southwestern Idaho during 1994 and 1995. Owls remained within natal areas for an average (± SE) of 58 ± 3.4 days post-hatching before moving permanently beyond 300 m, which was our operational cutoff for dispersal from the natal area. On average, owls dispersed on 27 July (range: 15 July to 22 August), which was approximately 4 weeks after fledging. After initiating dispersal, juveniles continued moving farther away from their natal burrows and, by 61-65 days post-hatching, they had moved 0.6 ± …

Rare, Threatened And Endangered Plants And Animals Of Oregon (2001), James S. Kagan, Sue Vrilakas, Eleanor P. Gaines, Cliff Alton, Ken Popper, Mark A. Stern, Eric Scheuering, John A. Christy, Mary Finnerty, Jon Hak, Anthony A. Tovar, Michael Murray, Claudine Tobalske, Oregon Natural Heritage Program

Institute for Natural Resources Publications

Extinction is a natural process. Today, however, plant and animal species are disappearing world-wide at an accelerated pace. Based on current trends, half of the species on earth will be extinct within the next 100 years. The major cause of this phenomenon is large-scale destruction of native habitats, which has increased since European settlement began in the mid 1800's - in Oregon and throughout the New World.

Once lost, a species can never be recovered, and there is no way of knowing how useful it may have been. We do know that human beings and many of their industries depend …

Semi-Artificial Monarch Butterfly Larval Diet, Kingston L. H. Leong

Biological Sciences

A semi-artificial diet of monarch butterfly larva and a process for making such a diet are provided. The diet consists of a substantial amount of dried milkweed powder and small but effective amounts of mold inhibitor and antibiotic.

The Tem1 Small Gtpase Controls Actomyosin And Septin Dynamics During Cytokinesis, John Lippincott, Katie Shannon, Wenying Shou, Raymond J. Deshaies, Rong Li

Biological Sciences Faculty Research & Creative Works

Cytokinesis in budding yeast involves an actomyosin-based ring which assembles in a multistepped fashion during the cell cycle and constricts during cytokinesis. In this report, we have investigated the structural and regulatory events that occur at the onset of cytokinesis. The septins, which form an hour-glass like structure during early stages of the cell cycle, undergo dynamic rearrangements prior to cell division: the hourglass structure splits into two separate rings. The contractile ring, localized between the septin double rings, immediately undergoes contraction. Septin ring splitting is independent of actomyosin ring contraction as it still occurs in mutants where contraction fails. …

Orientation In Complex Odor Landscapes: Spatial Arrangement Of Odor Sources Influences Crayfish Food Finding Efficiency In Artificial Streams, Troy Keller

Faculty Bibliography

Fluid dynamics has been shown to alter ecologically important behaviors of aquatic organisms orienting to distant chemical sources. Because the fluid dynamics and chemical plumes change across hydraulic environments, it is unclear which of these factors influence orientation behavior more. This study examined how alterations in chemical signal structure, through changes in source spatial arrangement, affect chemically mediated search behavior. Microelectrochemical measurements of tracer molecules revealed that source arrangement significantly alters the downstream fine-scale structure of chemical plumes. Flume hydrodynamic characterizations (as measured with laser Doppler velocimetry) also differed among source arrangements; however, differences were minor and existed only at …

Willa Cather And American Plant Ecology, Cheryl C. Swift, John N. Swift

Biology

The following argument began simply in our curiosity over Willa Cather's naming of flowers, particularly in her late work. Looking at these acts of naming, however, led us quickly to the beginnings of a more general historical/theoretical framework for thinking about her long relationship with natural science. Cather is of course well known as an acute botanical observer and literary user of the floral or vegetative world, as pastoral backdrop, as ornamental illustration, or as emblem. Susan Rosowski has moreover suggested a foundational "ecological dialectic" in all of her art, originating in her early university experiences with the pioneering Nebraska …

Analysis Of E. Coli Dna From Selected Source(S) Using Pulsed-Field Gel Electrophoresis, Sheema Doshi

Theses, Dissertations and Capstones

The presence of fecal coliforms in surface waters is a public health issue throughout the United States and Canada. The presence of Escherichia coli (E. coli) indicates recent fecal contamination from either animal or human sources. To solve this growing problem one must identify the source(s) of such contamination by creating a database of DNA profiles of£. coli isolates. Hence, tracking the source(s) of E. coli can help link water contamination to animal source(s) of contamination. In this study, pulsed field gel electrophoresis (PFGE) was used to analyze DNA from E. coli isolates from known animals. For PFGE, the bacterial …

Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle

Theses, Dissertations and Capstones

The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …

Characterization And Regulation Of Skn-Directed Autoimmune Skin Lesions, Pamela Jane Staton

Theses, Dissertations and Capstones

In the Skn model of autoimmunity, pathogenic T cells sensitized to Skn epidermal antigens (Skn-immune spleen cells or SIS cells) elicited disease in the form of skin lesions when adoptively transferred into shaved immunoincompetent syngeneic adult recipients (‘lesion-forming animals’) while concomitant injection of immunoregulatory normal spleen cells at twice the concentration (2 x normal spleen cells or 2XNS cells) of SIS cells significantly lessened lesion development (‘lesion-controlling animals’) by Day 7 post cell transfer. In a time-course RT-PCR cytokine analysis of skin from lesion-forming and lesion-controlling animals, IL-7 mRNA was significantly elevated in lesion-controlling skin on Day 7 while no …

A New Species Of Extinct Oryzomyine Rodent From The Quaternary Of Curaçao, Netherlands Antilles, Donald A. Mcfarlane, Adolphe O. Debrot

WM Keck Science Faculty Papers

An extinct new species of Oryzomyine rodent, known since 1936 from the cave of Grot van Hato on the island of Curaçao, is described from abundant new specimens collected from owl-pellet deposits at three localities on the island.

Apoptosis Of Rat Myoblasts Is Induced In Vitro By Late, But Not Early, Wound Fluids, Raymond E. Sicard, Linh Nguyen, John Menezes

Journal of the Minnesota Academy of Science

Interstitial fluids from late wound repair environments are not "regeneration permissive" as judged by their inability to promote either proliferation or differentiation of myoblasts in vitro. This irwestigation considered whether apoptosis (induced death) was an alternative fate for myoblasts exposed to these interstitial fluids. Myoblast fate was assessed by cell counts, tritiated thymidine release, and propidium iodide staining. Fluids from early wounds increased mean cell counts and induced little thymidine release or propidium iodide labeling. In contrast, fluids from late wounds reduced cell counts and induced both thymidine release and propidium iodide labeling. These data suggest that interstitial fluids from …

Nerve Injury Induces A Rapid Efflux Of Nitric Oxide (No) Detected With A Novel No Microsensor, Shanta M. Kumar, D. M. Porterfield, Kenneth J. Muller, Peter J.S. Smith, Christie L. Sahley

Biological Sciences Faculty Research & Creative Works

An early step in repair of the leech CNS is the appearance of endothelial nitric oxide synthase (eNOS) immunoreactivity and NOS activity, but coincident generation of NO at the lesion after injury has not been shown. This is important because NO can regulate microglial cell motility and axon growth. Indirect measurement of NO with the standard citrulline assay demonstrated that NO was generated within 30 min after nerve cord injury. A polarographic NO-selective self-referencing microelectrode that measures NO flux noninvasively was developed to obtain higher spatial and temporal resolution. With this probe, it was possible to demonstrate that immediately after …

Ethnic Differences In Risk From Mercury Among Savannah River Fishermen, Joanna Burger, Karen F. Gaines, Michael Gochfeld

Faculty Research & Creative Activity

Fishing plays an important role in people's lives and contaminant levels in fish are a public health concern. Many states have issued consumption advisories; South Carolina and Georgia have issued them for the Savannah River based on mercury and radionuclide levels. This study examined ethnic differences in risk from mercury exposure among people consuming fish from the Savannah River, based on site-specific consumption patterns and analysis of mercury in fish. Among fish, there were significant interspecies differences in mercury levels, and there were ethnic differences in consumption patterns. Two methods of examining risk are presented: (1) Hazard Index (HI), and …

Genetic Heterogeneity Of White-Tailed Deer: Management Lessons From A Long-Term Study, Michael H. Smith, James M. Novak, J. D. Peles, J. R. Purdue

Faculty Research & Creative Activity

Genetic data from a long-term (16-year) study of white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) were examined to evaluate spatial and temporal genetic heterogeneity in this species. Based on our analyses of the long-term data set, three major findings emerged, all of which have important implications for management of white-tailed deer: (1) There exists significant spatial genetic heterogeneity in white-tailed deer based on analyses of allozyme frequencies and mtDNA haplotypes. This heterogeneity exists on a much smaller spatial scale than would be expected for such a large and potentially mobile species as 0. …