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Modified Green Tea Polyphenol, Egcg-Ester, As A Novel Approach To Inhibit Herpes Simplex Virus Infections, Aline Moraes De Oliveira

Theses, Dissertations and Culminating Projects

HSV-1 DNA with the green fluorescent protein (GFP) introduced into the UL46 gene was used to investigate the effects of green tea polyphenols on the virus. Two different green tea extracts have been isolated and modified, EGCG and EGCG-ester. These extracts with concentration of 12.5, 25, 50, 75 and 100uM were used to determine its effects on cell morphology, cell proliferation and cell viability. The results indicated that the maximum non-toxic concentration is 75uM for both polyphenols. GFP expression, DAPI DNA stain and Lysosome activation stain were also used to observe the morphological, and cellular changes in the infected cells. …

Ectoparasites Of American Kestrel Nestlings In Northern New Jersey And Their Relationship To Nestling Growth And Breeding Success, Mark Joseph Lesko

Theses, Dissertations and Culminating Projects

American kestrel (Falco sparverius) populations throughout much of the United States have exhibited a substantial decline during recent years. Prompted by this decline, I designed this study to determine if removing arthropod parasites from nestlings was an effective strategy in increasing the breeding success of American kestrels, as well as to identify and quantify the ectoparasites found on nestlings. I conducted the study in Sussex and Warren counties in northwestern New Jersey in conjunction with a nest box program established and monitored by J. A. Smallwood and his students (hereafter referred to as we). I removed parasites periodically from all …

Effects Of Prescribed Burning On Grassland Nesting Birds On Conservation Reserve Program Areas In Gove County, Kansas, Justin Vern Hamilton

Master's Theses or Doctor of Nursing Practice

Great Plains grasslands were once one of the largest ecosystems in North America. However, farming, ranching, urban development, widespread fire suppression, and numerous other factors have created a great loss of this habitat in central North America. Organisms that depend on that habitat, such as grassland nesting birds, also have declined. The Conservation Reserve Program (CRP), which was established in 1985, paid landowners to remove land with highly erodible soils from production and plant it with perennial vegetation. Increases in CRP acreages brought about increases in numbers of several bird species that were in decline before the program existed. Prescribed …

Modeling Hemorrhagic Stroke And Vasospasm In The Rodent, Sarah Katherine Jane Wendel

Renée Crown University Honors Thesis Projects - All

In January 2010, I started work as an undergraduate research assistant at SUNY Upstate Medical University in Dr. Mary Lou Vallano’s laboratory in the Department of Neuroscience and Physiology. My research project was part of a collaborative effort with the laboratory of Dr. Eric Deshaies, a vascular neurosurgeon at Upstate. The goal was to establish a rodent model of subarachnoid hemorrhage and delayed vasospasm, and to test possible protective strategies. We used an adult rat model, in which two injections of autologous blood were given in the cisterna magna region of the brain. Analysis was done using a combination of …

Pathophysiology And Treatment Of Septic And Traumatic Shock, Lyuba Polinkovsky

Renée Crown University Honors Thesis Projects - All

Background. While various medical treatments have been proven effective in the treatment of sepsis in animal models, the only current clinically accepted treatment of human sepsis is limited to the use of activated protein C. The complex pathogenesis of human sepsis presents a challenge in precisely duplicating the process of disease development in the particular animal models which are currently employed for preclinical testing. Further research examining the pathogenesis of this deadly condition is essential for the implementation of novel therapies that target distinct disease mechanisms. The objective of this 48-hour study is to utilize a clinical model that accurately …

Relative Abundance Of Mitochondria Rich Cell Types In Threespine Stickleback: Interpopulation Differences And Salinity Effects, Dante Paolino

Honors Scholar Theses

The objective of this study is to determine whether there are evolutionary trade-offs among populations isolated to different salinities in nature with regards to mitochondria rich (MR) cell production. We expected differences among populations in MR cell abundances due to the energy requirements needed to maintain and produce these cells. Eggs from three populations of threespine stickleback (Gasterosteus aculeatus) were hatched and reared for 10 weeks and then the fish were put into one of two salinity challenges: either 0.4 ppt freshwater or 35 ppt saltwater for 13 days. The populations consisted of one isolated to freshwater, one isolated …

An Investigation Of The Composition, Microbial Digestion And Potential Applications Of An Industrial By-Product Of Seaweed Extraction, Juncal Nogales Esteban

Theses

Commercial preparation of seaweed extract from Ascophyllum nodosum, for use as fertilizer and soil improver, produces a residue which requires remediation. This residue is rich in nutrients and offers potential for other added-value products. The residue’s composition and microbial flora were studied, a microbial digestion system was developed, and extracts were screened for anti-hyaluronidase and anti-elastase activities.

The residue had a pH of 8.61±0.39, 16% (w/w) TS which comprised 40.6% minerals, 29.5% fibre, 20.9% lipid, 4.9% protein and 0.48% polyphenols. The microbial digestion included an initial 3-day anaerobic phase during which pH decreased from 9.12 to 7.89. At day 3, …

Natural Selection And Age-Related Variation In Morphology Of A Colonial Bird, Mary Bomberger Brown

School of Natural Resources: Dissertations, Theses, and Student Research

In May 1996, inclement weather led to the deaths of thousands of Cliff Swallows (Petrochelidon pyrrhonota) in Nebraska. Survivors had larger skeletons, shorter wings and tails, and less wing asymmetry than non-survivors. This population was followed for 10 years to study 1) whether natural selection events result in permanent microevolutionary changes, 2) if variation in climate affects the development of morphological traits, and 3) if morphological traits vary systematically with age.

Patterns in morphology exhibited by swallows following the selection event were studied by measuring yearling birds. Wing and middle tail lengths decreased, beak length and width increased, …

Homocysteine: A Potential Molecular Link Between Alzheimer's Disease And Type 2 Diabetes Mellitus, Cyndel Carreau

Biological Sciences

In order to elucidate some of the mechanisms through which Alzheimer’s disease (AD) and Type II Diabetes Mellitus (T2DM) are linked, this study investigated the effects of elevated plasma homocysteine levels – a risk factor for AD – in a rat model of T2DM. Both elevated plasma homocysteine levels and T2DM are associated with cognitive deficits and are recognized as strong risk factors for the development of AD. The present experiment examined the effects of diet-induced hyperhomocysteinemia on the development of cognitive impairments and insulin-resistance, as well as on the insulin signaling cascade, in a diet-induced obese rat model of …

Behavioral Aversion Of Two Ranid Frogs To Road Deicers : Does Terrestriality Influence Sensitivity?, Erika Koelmel

Theses, Dissertations and Culminating Projects

Amphibian decline has been a global phenomenon in recent decades due in part to negative human effects on the environment. Sensitivity of amphibians to environmental degradation makes them good indicators of environmental change, attesting to the importance of amphibian inventory and population monitorings De-icing salts, such as sodium chloride, are applied to roads during the winter months and may accumulate in aquatic and terrestrial habitats. The exposure of amphibians to high salt concentrations can impair their physiological functioning due to their highly permeable skin. The purpose of this investigation is to examine the behavioral tolerance threshold for road salt (NaCl) …

Carbon And Nitrogen Stable Isotopes In Fruits And Arthropods That Are Eaten By Songbirds During Migration, Sarah E. Donlan

Senior Honors Projects

The use of stable isotope analysis is becoming more common in all areas of animal ecology. In particular, natural variation in the abundance of stable isotopes makes them useful in studies of foraging ecology. For example, nitrogen stable isotopes can offer insight into trophic relationships and carbon stable isotopes tend to trace the original source of carbon in a system (e.g. - photosynthetic pathway).  This natural variation is potentially useful for quantifying resource use because the isotopic composition of an animal’s diet is assimilated somewhat predictably into the animal’s tissues. In fact, the ratio of naturally-occurring isotopes in animal tissues …

Calcium Provision In A Placentotrophic Lizard: Structural Differentiation Reflects Functional Specialization., Haley K. Stinnett

Undergraduate Honors Theses

Viviparity (live birth) and placentation have evolved in more than 100 lineages of squamate reptiles. However, highly placentotrophic species in which embryos receive the majority of nutrients for development via maternal transport across a placenta are rare. Pseudemoia pagenstecheri is a viviparous Australian scincid lizard with extensive placental transfer of nutrients. For example, 90% of neonate calcium is received via placental transfer. This species has a regionally differentiated chorioallantoic placenta distinguished by an elliptical-shaped region, the placentome. The placentome is characterized by hypertrophied uterine and embryonic epithelial cells supported by dense vascular networks. The remainder of the chorioallantoic placenta is …

Identification, Cloning And Fluorescent-Tagging Of Protamines Of The Flour Beetle, Tribolium Castaneum, Kristina Martimucci

Renée Crown University Honors Thesis Projects - All

The genome of eukaryotes is associated with chromosomal proteins that fold the DNA into ordered arrangements. The most abundant of these are the histones. Four of these (H2A, H2B, H3 and H4) form the nucleosomal core around which DNA is wrapped. Other proteins can associate with chromatin and fold it into higher order structures. During spermatogenesis in many organisms the standard chromatin configuration undergoes a transition to a highly condensed form in which small, highly basic proteins called protamines replace the histones. It is thought that protamine-based chromatin is more resistant to mutational damage.

Previous work by the Renkawitz-Pohl group …

Design Of A Factorial Experiment With Randomization Restrictions To Assess Medical Device Performance On Vascular Tissue, Wiebke Diestelkamp, Carissa M. Krane, Margaret Pinnell

Biology Faculty Publications

Background: Energy-based surgical scalpels are designed to efficiently transect and seal blood vessels using thermal energy to promote protein denaturation and coagulation. Assessment and design improvement of ultrasonic scalpel performance relies on both in vivo and ex vivo testing. The objective of this work was to design and implement a robust, experimental test matrix with randomization restrictions and predictive statistical power, which allowed for identification of those experimental variables that may affect the quality of the seal obtained ex vivo.

Methods: The design of the experiment included three factors: temperature (two levels); the type of solution used to perfuse the …

Endo-Porter–Mediated Delivery Of Phosphorodiamidate Morpholino Oligos (Pmos) In Erythrocyte Suspension Cultures From Cope's Gray Treefrog Hyla Chrysoscelis, Venkateshwar Mutyam, Matthew V. Puccetti, James Frisbie, David L. Goldstein, Carissa M. Krane

Biology Faculty Publications

Cope's gray treefrog, Hyla chrysoscelis, is a freeze-tolerant anuran that accumulates cryoprotective glycerol during cold acclimation. H. chrysoscelis erythrocytes express the aquaglyceroporin HC-3, which facilitates transmembrane glycerol and water movement. Aquaglyceroporins have no pharmacological inhibitors, and no genetic knockout tools currently exist for H. chrysoscelis. A phosphorodiamidate morpholino oligo (PMO)–mediated expression knockdown approach was therefore pursued to provide a model for testing the role of HC-3. We describe a novel procedure optimized for specific, efficient knockdown of HC-3 expression in amphibian erythrocyte suspensions cultured at nonmammalian physiological temperatures using Endo-Porter. Our protocol includes three critical components: pre-incubation at 37°C, …

Cop 9 Signalosome Subunit 6 Stabilizes Cop1, A Novel E3 Ubiquitin Ligase For 14-3-3Σ, Hyun Ho Choi

Dissertations and Theses (Open Access)

14-3-3σ, a gene upregulated by p53 in response to DNA damage, exists as part of a positive-feedback loop which activates p53 and is a human cancer epithelial marker downregulated in various cancer types. 14-3-3σ levels are critical for maintaining p53 activity in response to DNA damage and regulating signal mediator such as Akt. Here, we identify Mammalian Constitutive Photomorphogenic 1 (COP1) as a novel E3 ubiquitin ligase for targeting 14-3-3σ through proteasome degradation. We show for the first time that COP9 signalosome subunit 6 (CSN6) associates with COP1 and is involved in 14-3-3σ ubiquitin-mediated degradation. Mechanistic studies show that CSN6 …

Investigating The Effects Of Silencing Epha2 In Metastatic Breast Cancer Cells, Stephanie Erzinger

Dissertations and Theses (Open Access)

EphA2, also known as ECK (epithelial cell kinase), is a transmembrane receptor tyrosine kinase that is commonly over-expressed in cancers such as those of the prostate, colon, lung, and breast. For breast cancers, EphA2 overexpression is most prominent in the ER-negative subtype, and is associated with a higher rate of lung metastasis. Studies conducted to demonstrate the role of EphA2 in a non-cancerous environment have shown that it is very important in developmental processes, but not in normal adult tissues. These results make EphA2 a prospective therapeutic target since new therapies are needed for the more aggressive ER-negative breast cancers. …

Characterization Of The Phytochelatin Synthase Of Schistosoma Mansoni, Debalina Ray, David L. Williams

Faculty Publications – Biological Sciences

Treatment for schistosomiasis, which is responsible for more than 280,000 deaths annually, depends exclusively on the use of praziquantel. Millions of people are treated annually with praziquantel and drug resistant parasites are likely to evolve. In order to identify novel drug targets the Schistosoma mansoni sequence databases were queried for proteins involved in glutathione metabolism. One potential target identified was phytochelatin synthase (PCS). Phytochelatins are oligopeptides synthesized enzymatically from glutathione by PCS that sequester toxic heavy metals in many organisms. However, humans do not have a PCS gene and do not synthesize phytochelatins. In this study we have characterized the …

A Study On The Applications And Toxicity Assessments Of Carbon Nanotubes In Tissue Engineering, Rena Baktur

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Carbon nanotubes (CNTs) are one of the most popular nanomaterials. There has been increasing interest in the development and applications of carbon nanotubes due to their huge potential in industrial and medical applications. Recent applications of carbon nanotubes include development of scaffolds and drug delivery systems. Despite rapidly emerging applications of CNTs, little is known about the impact of CNTs on cellular processes, especially mesenchymal stem cell (MSC)'s differentiation. Also, the effects of nanoparticle exposure under different conditions on cellular responses have not been well characterized yet.

To characterize the effects of CNTs on creating nanoscale scaffolds for tissue engineering, …

Molecular Evolution And Historical Biogeography Of New World Birds, Brian T. Smith

UNLV Theses, Dissertations, Professional Papers, and Capstones

Deciphering the patterns of how biodiversity has evolved across time and space has remained a fundamental objective for biologists for the last 200 years. Researchers are faced with the challenge of interpreting the complexity of evolutionary patterns that have been generated over the deep history of the Earth. The advancement of DNA sequencing technology has yielded a new and powerful genetic toolkit that has allowed biologists to address novel evolutionary questions. For my dissertation research, I used molecular genetics and a statistical framework to study the evolution and historical biogeography of birds distributed in North and South America. My dissertation …

Second-Order Conditioning In Drosophila, Christopher J. Tabone

UNLV Theses, Dissertations, Professional Papers, and Capstones

Animals possess the ability to associate neutral stimuli in their environment with both rewards and punishment. A conditioned stimulus (CS1) such as a smell or sound, can become associated with an unconditioned stimulus (US), such as a food reward, to elicit what is known as the conditioned response (CR). This type of learning is commonly referred to as classical conditioning or first-order conditioning (FOC). Second-order conditioning (SOC) is an extension of this type of association wherein a novel stimulus is introduced (CS2) and associated with a previously conditioning first-order stimulus (CS1). As a result, the organism may show an attraction …

Phylogeography Of Three Heteromyid Taxa: Insight On The Evolution Of A North American Arid Grassland Rodent Guild, Sean A. Neiswenter

UNLV Theses, Dissertations, Professional Papers, and Capstones

Revealing how communities are shaped by abiotic and biotic factors plays a central role in biogeographic and comparative phylogeographic studies. The biogeography of North American arid grasslands is explored using nuclear and mitochondrial DNA from three groups of heteromyid rodents that are broadly sympatric in aridlands across western North America. Phylogenetic and molecular clock analyses are used to estimate the timing of divergences within each group. A general pattern of late Miocene divergence and expansion of lineages in each of the groups that is coincident with the rapid expansion of arid grasslands at the time. The initial divergence is followed …

Functional Classification Of Divergent Protein Sequences And Molecular Evolution Of Multi-Domain Proteins, Pooja K. Strope

School of Biological Sciences: Dissertations, Theses, and Student Research

Transmembrane proteins and multi-domain proteins together make up more than 80% of the total proteins in any eukaryotic proteome. Therefore accurately classifying such proteins into functional classes is an important task. Furthermore, understanding the molecular evolution of multi-domain proteins is important because it shows how various domains fuse to form more complex proteins, and acquire new functions possibly affecting the organismal level of evolution. In this thesis, I first investigated the performance of several protein classifiers using one of the most divergent transmembrane protein families, the G-protein-coupled receptor (GPCR) superfamily, as an example. Alignment-free classifiers based on support vector machines …

Eugenics And Modern Biology: Critiques Of Eugenics, 1910-1945, Garland E. Allen

Biology Faculty Research

Eugenics in most western countries in the first four decades of the twentieth century was based on the idea that genes control most human phenotypic traits, everything from physical features such as polydactyly and eye color to physiological conditions such as the A-B-O blood groups to mental and personality traits such as “feeblemindedness”, alcoholism and pauperism. It assessing the development of the eugenics movement – its rise and decline between 1900 and 1950 – it is important to recognize that its naïve assumptions and often flawed methodologies were openly criticized at the time by scientists and non-scientists alike. This paper …

From Growth To Extinction: Explored By Life History And Metabolic Theory, Wenyun Zuo

Biology ETDs

The laws of energy and material conservation are fundamental principles across various scales and systems. Based on the conservation laws, I derive several theoretical models to understand mechanisms behind the energy budget of ontogenetic growth and the pattern of the late Pleistocene extinction of megafauna in the Americas. First, I present a model, empirically grounded in data from birds and mammals, that correctly predicts how growing animals allocate food energy between synthesis of new biomass and maintenance of existing biomass. Previous energy budget models have typically been based on rates of either food consumption or metabolic energy expenditure. The model …

Stable Isotopes Provide Insight Into The Use Of Wildlife Water Developments By Resident And Migrant Birds In The Sonoran Desert Of Arizona, Theresa C. Hyde

Biology ETDs

Several studies have looked at the importance of man-made water resources to wildlife in desert regions. To our knowledge, however, none have attempted to directly quantify their importance to both resident and Neotropical migratory birds. During the spring and summer from 2007-2009, we enriched man-made water developments in the Sonoran Desert on the Kofa National Wildlife Refuge, Arizona. We enriched water developments using deuteriated water and sampled the body water pools of resident and migrant birds to quantify development use. We used a simple two end-point mixing model to estimate the proportion of an individual birds body water pool that …

Divergence In The Ecology Of Two Species Of Gambusia In Secondary Contact, Daniella Marilyn Swenton

Biology ETDs

Gambusia nobilis, a federally endangered species, and G. affinis (Poeciliidae) are small, livebearing fishes found in the southwestern U.S. The invasive G. affinis has been introduced globally to control mosquito populations. It is found in some populations of G. nobilis on Bitter Lake National Wildlife Refuge (BLNWR), the field site for this study. It is unclear to what extent the two species have diverged in behavior and ecology and how extensively they have hybridized on BLNWR, thereby threatening the endangered G. nobilis via genetic introgression. In this study I examined divergence in behavior and ecology between two species with four …

A Contribution To The Vascular Flora Of The Sequatchie Valley Within Sequatchie County, Tennessee, John Richard Evans

Masters Theses and Doctoral Dissertations

The vascular flora of the Sequatchie Valley within Sequatchie County, Tennessee was studied for three growing seasons from 2008 through 2010. The Sequatchie Valley is located within the southeastern portion of the Cumberland Plateau and is generally considered to be part of the Cumberland Plateau Physiographic Province. Nevertheless, a number of geological and ecological factors distinguish the Sequatchie Valley from the elevated Cumberland Plateau, suggesting that the floristic composition of the valley may be different from the surrounding region. Although several previous floristic studies have been conducted on the plateau surface, very little attention has been given to the Sequatchie …

Passive Immunization Reduces Behavioral And Neuropathological Deficits In An Alpha-Synuclein Transgenic Model Of Lewy Body Disease, Troy Rohn

Biology Faculty Publications and Presentations

Dementia with Lewy bodies (DLB) and Parkinson's Disease (PD) are common causes of motor and cognitive deficits and are associated with the abnormal accumulation of alpha-synuclein (α-syn). This study investigated whether passive immunization with a novel monoclonal α-syn antibody (9E4) against the C-terminus (CT) of α-syn was able to cross into the CNS and ameliorate the deficits associated with α-syn accumulation. In this study we demonstrate that 9E4 was effective at reducing behavioral deficits in the water maze, moreover, immunization with 9E4 reduced the accumulation of calpain-cleaved α-syn in axons and synapses and the associated neurodegenerative deficits. In vivo studies …

A Tutorial Of Manual Segmentation Using The Visualization Program Avizo® As Illustrated By The Skeleton Of The Spiny Dogfish Shark, Squalus Acanthias, Mateusz A. Lapucha

Honors Capstones

No abstract provided.