Digital Commons Network^™

Pcr Amplification Of Trypanosoma Cruzi - Specific Dna From Raccoon Blood Samples, Colin Moss

Mahurin Honors College Capstone Experience/Thesis Projects

The parasitic protist Trypanosoma cruzi is the causative agent of Chagas Disease. Chagas Disease causes greater than 15,000 deaths each year, and nearly 28 million people are believed to be at risk of infection in Central and South America. This parasite has been described in many mammalian host species and has also been described in the United States. The purpose of this study was to attempt to use PCR to amplify T. cruzi-specific DNA directly from blood samples obtained from raccoons (Procyon lotor) trapped in Warren and Barren Counties of Kentucky in 2007 and 2008. DNA was …

Mitochondrial Dna Inheritance In Bivalves: A Comparative Study Involving The Unique System Of Doubly Uniparental Inheritance, Sarah Heishman

Honors Theses

A comparative study of Doubly Uniparental Inheritance (DUI) in the bivalve mollusks Mytilus edulis and Geukensia demissa has yielded unanticipated results. Around the world, it has been reported that members of the taxonomic family Mytilidae (along with the families of Unionidae and Veneridae) consistently exhibit DUI. However, the hard-to-place Geukensia demissa, which is a member of this family, has had varying reports of its DUI status. Most reports involving G. demissa vary with the location in which it is being studied, which prompts more questions than it answers. Due to many months of unsuccessful DNA purification attempts, sequencing and an …

Effects Of Exposure To Low, Ecologically Relevant Doses Of Atrazine On Somatic And Gonadal Development In American Toad (Bufo Americanus) Tadpoles, Tyler Davis Hoskins

Undergraduate Honors Thesis Collection

Atrazine is the most commonly used herbicide in the United States, with 80 million pounds applied annually, making it the most common contaminant of ground and surface water nationwide. It has been shown to act as a potent endocrine disrupter in amphibians, causing altered somatic and gonadal development in the ecologically relevant part per billion range; as a result, it has been hypothesized that atrazine may be a major factor behind amphibian declines. However, responses of different species to the chemical vary widely, and have made predicting susceptibility difficult. Recently, it has been shown that life history can serve as …

Assessing Social Benefits Of Domestic Rainwater Harvesting In Southern Kenya, Lindsey M. Filiatreau

Mahurin Honors College Capstone Experience/Thesis Projects

In the Kasigau location of the Voi District of Kenya, severe rainwater shortages closed fourteen water collection stations in 2008 and 2009, leaving ten water sources to service 15,000 people in the region. Few families in the area have domestic rainwater harvesting systems which are an easily implemented, low-cost means of water collection. I investigated the ability of rainwater catchment systems to modify family time budgets (by reducing water collection time) and lessen the severity of water scarcity (by supplying families a significant amount of water). Forty families participated in the research, 20 of whom received free installation of a …

Once On The Dark Continent, Katrina Alexandria Bidwell

Mahurin Honors College Capstone Experience/Thesis Projects

Set in the fictional village of Buguta in post-genocide Rwanda, Once on the Dark Continent follows the experiences of a young doctor working for the Doctors Without Borders Program. Dr. Erica Davis unwittingly finds herself in a country where the past has continued to repeat itself a decade after a devastating civil war, unbeknownst to most of the outside world. Their history isn’t the only thing plaguing the villagers like a parasite: Erica’s careful attention to the patients at the Buguta clinic leads her to diagnose them with toxoplasmosis, a revelation that has the potential to begin solving the myriad …

Transcriptional Regulation Of Nitrogenase And Identification Of A Fructose Transport System In Anabaena Variabilis, Justin Lynn Ungerer

Dissertations

Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied using a lacZ reporter. Two promoters were found to be responsible for expression of nifHDK; a weak promoter within nifU1 and a strong promoter upstream of nifB (the nifB1 promoter). The region immediately upstream of vnfH did not drive expression of lacZ, however the region that included the promoter for the upstream gene, ava4055 did. Characterization of the previously reported nifH1 and vnfH transcriptional start sites by 5' …

Avian Abundance And Diversity In Crep And Fescue Fields In The Summer And Winter Related To Vegetation Structure In The Upper Green River Watershed, Ky, Thomas A. Hulsey

Mahurin Honors College Capstone Experience/Thesis Projects

Grassland bird populations have been undergoing dramatic declines in the last fifty years due to loss and degradation of habitat. The Conservation Reserve Enhancement Program (CREP) was initiated in the upper Green River Watershed of south-central Kentucky to improve water quality and provide wildlife habitat. Many studies have previously examined how similar programs in other states have contributed to grassland bird populations; in this study we are examining how the Kentucky CREP program is influencing bird populations, particularly grassland birds, and contributing to the conservation of these species. Bird and vegetation surveys were conducted during the summer of 2008 and …

Screening Rnai Transformants Of Chlamydomonas For Reduced Expression Of The Photoreceptor Cryptochrome, Jeremy Webb

Mahurin Honors College Capstone Experience/Thesis Projects

Chlamydomonas reinhardtii is a unicellular green alga. In C. reinhardtii, three types of photoreceptors are known to be present: rhodopsins, phototropins, and cryptochromes. The single cryptochrome is the most likely photoreceptor for adjusting the circadian clock to the daily light/dark cycles, because cryptochromes are involved in clock entrainment in higher plants and insects. In this segment of the research, C. reinhardtii strains, which were genetically modified through transformation with a RNA interference construct, were screened for reduction in cryptochrome compared to the control strain. After C. reinhardtii cultures were harvested in complete darkness, all soluble proteins were extracted from the …

Role Of Stress In The Onset Of Diabetes Mellitus In Mice., Lindsey Brianne Mcamis

Undergraduate Honors Theses

This study investigated the effects of stress on the onset of chemically-induced diabetes mellitus in mice. Sixty male ICR mice were randomly divided into 6 groups of 10 mice. A factorial arrangement of 2 factors (streptozotocin (STZ) and stress) was used to produce 6 treatments. Three levels of STZ (0, 25 and 50 mg/kg bodyweight) and 2 levels of stress (stress and no-stress) were used. Stressed groups were exposed to restraint stress 6 hours daily for 18 days. Mice bodyweight, feed consumption and blood glucose levels were recorded regularly during the study period. At the end of the study period, …

Ontogeny Of Postcranial Morphology And Locomotor Behavior In Propithecus Verreauxi Coquereli And Lemur Catta, Stephanie Ann Wolf

Masters Theses, 2010-2019

Despite living under the same environmental pressures and sympatrically in many cases, Propithecus verreauxi and Lemur catta have evolved very different strategies for survival in stochastic environmental conditions. P. verreauxi show slow somatic growth, low maternal investment, and rapid dental growth while L. catta show faster somatic growth, high maternal investment, and slower dental growth. P. v. coquereli are highly specialized for vertical clinging and leaping (VCL) among lemurs, while L. catta, the most terrestrial of lemurs, use a wider variety of locomotor types including quadrupedalism, climbing, and leaping. P. v. coquereli have unusually long legs and muscular thighs while …

Interleukin-3 Promotes Susceptibility In A Mouse Model Of Cutaneous Leishmaniasis, Bryan Lewis Saunders

Masters Theses, 2010-2019

Cutaneous leishmaniasis (CL), a vector-borne infectious disease caused by protozoan parasites of the genus Leishmania, is one of the most important neglected infectious diseases worldwide. Currently, 10 million people in 82 (mostly developing) countries are infected. Those infected develop ulcerative skin lesions on exposed parts of the body, causing serious disability and permanent scarring. All mice are susceptible to infection with Leishmania major, however, the outcome of infection is different depending on the mouse strain. For example, resistant C57BL/6 mice develop lesions, which like in humans, ultimately heal. In contrast, lesions in susceptible BALB/c mice progressively worsen, ultimately resulting in …

A Taxonomic Revision Of The Endemic Members Of Varronia P. Br. (Cordiaceae) In The Galápagos Islands, Julia Kathryn Stutzman

Masters Theses, 2010-2019

The Galápagos Islands have long been an arena for biological diversity, scientific discovery, and conservation. Accurate identification and documentation of the flora of the Galápagos continue to aid conservation efforts. The purpose of this study was to conduct a taxonomic revision of the endemic members genus Varronia (Cordiaceae) on the Islands: V. revoluta (Hook. f.) Andersson, V. leucophlyctis (Hook. f.) Andersson, V. canescens Andersson, and V. scouleri (Hook. f.) Andersson. Taxonomic uncertainty among these species has resulted in difficult evaluation of their potential for conservation status by the International Union for the Conservation of Nature. The present taxonomic study concludes …

Human-Wildlife Conflict On Small, Subsistence Farms In Kenya, Christopher B. Colonna

Mahurin Honors College Capstone Experience/Thesis Projects

As human populations expand, wildlife suddenly competes with humans for resources and confrontation arises as a result. Rural Africa is typical of this problem. We surveyed local owners of small farms within the five villages surrounding Mount Kasigau in Southeast Kenya to quantify losses due to wildlife depredation on both subsistence and cash crops as well as to discover the patterns and variables influencing farmer-wildlife confrontations in the region. We found no statistically significant correlations among the value of damage per acre, the distance from the bush, or the distance to the nearest water source. We did find statistical significance …

Septin Filaments Exhibit A Dynamic, Paired Organization That Is Conserved From Yeast To Mammals, Bradley S. Demay, Xiaobo Bai, Louisa Howard, Patricia Occhipinti, Rebecca A. Meseroll, Elias T. Spiliotis, Rudolf Oldenbourg, Amy S. Gladfelter

Dartmouth Scholarship

The septins are conserved, GTP-binding proteins important for cytokinesis, membrane compartmentalization, and exocytosis. However, it is unknown how septins are arranged within higher-order structures in cells. To determine the organization of septins in live cells, we developed a polarized fluorescence microscopy system to monitor the orientation of GFP dipole moments with high spatial and temporal resolution. When GFP was fused to septins, the arrangement of GFP dipoles reflected the underlying septin organization. We demonstrated in a filamentous fungus, a budding yeast, and a mammalian epithelial cell line that septin proteins were organized in an identical highly ordered fashion. Fluorescence anisotropy …

The History Of Zoological Gardens And The State, Federal And International Laws That Govern Them, Morgan Maxwell

Mahurin Honors College Capstone Experience/Thesis Projects

The history of zoos in North America can be traced back through 18th century Europe to ancient civilizations such as the Romans. The menageries of ancient Rome were far different from modern zoos that stress conservation, education, and research as primary objectives. Zoos are still evolving and changing to become more suitable for captive animals and to ensure their animals’ health and well-being. An examination of state wildlife laws reveals that there are more laws that pertain to wildlife at the state and local level than at any other level of government. Federal wildlife laws are few in number and …

A General Life History Theory For Effects Of Caloric Restriction On Health Maintenance, Chen Hou, Kendra Bolt, Aviv Bergman

Biological Sciences Faculty Research & Creative Works

Background: Caloric restriction (CR) has been shown to keep organisms in a relatively youthful and healthy state compared to ad libitum fed counterparts, as well as to extend the lifespan of a diverse set of organisms. Several attempts have been made to understand the underlying mechanisms from the viewpoint of energy tradeoffs in organisms' life histories. However, most models are based on assumptions which are difficult to justify, or are endowed with free-adjusting parameters whose biological relevancy is unclear.

Results: In this paper, we derive a general quantitative, predictive model based on physiological data for endotherms. We test the hypothesis …

Functional Divergence Of Glycoprotein Hormone Receptors, Mihael Freamat, Stacia A. Sower

New Hampshire Agricultural Experiment Station Publications

Two lamprey glycroprotein hormone receptors (lGpH-R I and II) highly similar with gnathostome GpH-Rs were cloned from sea lamprey testes and thyroid, respectively. Vertebrate glycoprotein protein receptors have a large extracellular domain (ED) containing a leu rich domain (LRD) linked to a rhodopsin-like transmembrane domain (TMD) through a highly divergent linker region (signal specificity domain, SSD or 'hinge' region) and a third major segment, the intracellular domain. To determine the potential roles of the different domains in the activation of the receptor following ligand-receptor binding, functional assays were performed on lGpH-R I/rat luteinizing hormone (LH)-R domain swapped chimeric receptors. These …

The Cell Biology Of Multi-Nucleated Giant Cell Formation, Johnathan L. Bartee

Theses

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Generation Of Transgenic Medicago Sativa Overexpressing "Osmotin-Chitinase" Gene Chimera, Jahnavi Reddy Kancharla

Masters Theses & Specialist Projects

Medicago is widely used as a forage crop. It is often susceptible to various pathogenic infections and exhibits low growth in drought and extreme climatic conditions. In the current study, a strategy was developed for over-expressing an “Osmotin-Chitinase” gene chimera in transgenic Medicago that could potentially confer resistance to different biotic and abiotic stresses. Seed germination of several cultivars of Medicago (M. sativa ssp. sativa, M. sativa ssp. falcata, M. sativa ssp. caerulea, M. truncatula, and M. Rugosa) was tested to determine the cultivars with good germination rates. Among these, M. sativa ssp. sativa showed an …

Drosophila Model For Fetal Alcohol Syndrome Disorders: Role For The Insulin Pathway, Rachael L. French, K D. Mcclure, U Heberlein

Faculty Publications, Biological Sciences

Prenatal exposure to ethanol in humans results in a wide range of developmental abnormalities, including growth deficiency, developmental delay, reduced brain size, permanent neurobehavioral abnormalities and fetal death. Here we describe the use of Drosophila melanogaster as a model for exploring the effects of ethanol exposure on development and behavior. We show that developmental ethanol exposure causes reduced viability, developmental delay and reduced adult body size. We find that flies reared on ethanol-containing food have smaller brains and imaginal discs, which is due to reduced cell division rather than increased apoptosis. Additionally, we show that, as in mammals, flies reared …

Neuronostatin Acts In Brain To Biphasically Increase Mean Arterial Pressure Through Sympatho-Activation Followed By Vasopressin Secretion: The Role Of Melanocortin Receptors, Gina L.C. Yosten, Alicia T. Pate, Willis K. Samson

Biological Sciences Faculty Research & Creative Works

Neuronostatin is a recently described neuropeptide that is derived from the somatostatin preprohormone. We have shown previously that neuronostatin led to a biphasic, dose-related increase in mean arterial pressure when injected into the lateral cerebroventricle of adult, male rats. Because neuronostatin depolarized both magnocellular and parvocellular, paraventricular nucleus neurons in hypothalamic slice preparations, we hypothesized that neuronostatin elevated mean arterial pressure first by stimulating sympathetic nervous system activity followed by the release of a pressor hormone, specifically vasopressin. We found that the first phase of neuronostatin-induced increase in mean arterial pressure was reversed by pretreatment with phentolamine, indicating that phase …

Cuttlefish Learning Efficiency In A Food Retrieval Task, Jasmine Schonwald

Biology Theses

Class Cephalopoda is thought to contain the most intelligent invertebrates thus far discovered. It has been found that cuttlefish have the capacity for learning (Boal et al. 2000, Darmillaq et al. 2003, Darmillaq et al 2006, Graindorge et al., 2006; Karson, Boal and Hanlon, 2003). It has also been shown that cuttlefish can navigate in sophisticated ways, such as within a maze (Graindorge, et al., 2006; Karson, Boal and Hanlon, 2003). The goal of this experiment is to determine the correlation between learning and navigation in cuttlefish. Cuttlefish were placed in an oval tank divided into …

Developing Mechanisms For The Transfer And/Or Adjustment Of Rock Lobster Shares Between Sectors In Western Australia And South Australia, Department Of Fisheries

Fisheries Management Papers

No abstract provided.

Thinking Outside The Channel: Modeling Nitrogen Cycling In Networked River Ecosystems, Ashley M. Helton, Geoffrey C. Poole, Judy L. Meyer, Wilfred M. Wollheim, Bruce J. Peterson, Patrick J. Mulholland, Emily S. Bernhardt, Jack Arthur Stanford, Clay Arango, Linda R. Ashkenas, Lee W. Cooper, Walter K. Dodds, Stanley V. Gregory, Robert O. Hall Jr., Stephen K. Hamilton, Sherri L. Johnson, William H. Mcdowell, Jody D. Potter, Jennifer L. Tank, Suzanne M. Thomas, H. Maurice Valett, Jackson R. Webster, Lydia Zeglin

Biological Sciences Faculty Publications

Agricultural and urban development alters nitrogen and other biogeochemical cycles in rivers worldwide. Because such biogeochemical processes cannot be measured empirically across whole river networks, simulation models are critical tools for understanding river-network biogeochemistry. However, limitations inherent in current models restrict our ability to simulate biogeochemical dynamics among diverse river networks. We illustrate these limitations using a river-network model to scale up in situ measures of nitrogen cycling in eight catchments spanning various geophysical and land-use conditions. Our model results provide evidence that catchment characteristics typically excluded from models may control river-network biogeochemistry. Based on our findings, we identify important …

Modular Inheritance Of Color Pattern In Lake Malawi Cichlid Fishes, Jennifer Roscoe

Renée Crown University Honors Thesis Projects - All

This study attempts to elucidate the mechanisms that underlie the evolution of pigmentation patterns among Lake Malawi cichlids. For decades researchers have sought to provide a theoretical model of cichlid speciation based on the evolution of male nuptial patterns and female preferences, yet our understanding of how color patterns change is limited. Here, we performed exploratory statistical analyses on an F₂ hybrid population to characterize the inheritance of color traits. Specifically, we sought to uncover patterns of modularity and levels of integration among color traits. Because cichlids are sexually dimorphic we did our analyses on males and females separately. …

Characterization And Application Of Dynamic In Vitro Models Of Human Airway, Hemangkumar J. Patel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In recent years, respiratory diseases have emerged as a leading cause of mortality across the globe. In the United States alone respiratory diseases are the fourth leading cause of deaths annually. Moreover, with the rapid increase of industrialization and urbanization, the occurrences of respiratory diseases are expected to remain high with strong chances of increasing in the future. To ameliorate the epidemic of respiratory disease, it is first important to understand its underlying mechanisms.

Respiratory research studies in animals have elucidated the chronological order of the pathological events and systemic responses inside the lung, but understanding the response of individual …

Testing The Utility Of The Consortium For The Barcoding Of Life's Two 'Agreed Upon' Plant Dna Barcodes, Matk And Rbcl, Iam Michael Cohen

Masters Theses and Doctoral Dissertations

DNA barcoding is the use of short standardized regions of DNA to identify unknown specimens to species. Currently, the zoological community has agreed that cytochrome oxidase I subunit 1 (COI), a mitochondrial gene region, will serve as the barcode region for all animal taxa. Due to oftentimes complicated evolutionary histories of plants, the plant barcoding community has had a much harder time agreeing on a gene region or regions that should be used to barcode the various land plant lineages. This is in large part due to poor reproductive barriers, which allows for chloroplast sharing between closely related species. In …

The Function And Mechanism Of Rrf-1 In Antiviral Rnai In C. Elegans, Amanda Lynn Callegan

Honors Capstones

No abstract provided.

Cloning And Characterization Of The Promoter For The Ovarian-Specific Inhibin Alpha Gene Form Zebrafish (Danio Rerio), Caitlin Marie Ament

Theses, Dissertations and Culminating Projects

Inducible cAMP Early Repressor (ICER) is an important regulator of folliculogenesis as it regulates the nuclear response to gonadotropins in ovarian tissue. Studies in mice demonstrated a 3.0 kb region of the alpha inhibin (INHa) promoter is a site of transcriptional activation in response to follicle-stimulating hormone (FSH). The purpose of this study was to isolate and construct an ICER transgene and characterize the putative 3.0 kb promoter region of the ovarian-specific INHa gene in Danio rerio (zebrafish).

The sequences for ICER and the INHa promoter in zebrafish were identified using BLAST searches with the known sequences from mouse and …

Combining Bioinformatics And Chemical Biological Approaches To The Study Of Signaling Pathways In Parasitic Nematodes, William R. De Martini

Theses, Dissertations and Culminating Projects

Lymphatic filariasis (elephantitis) is a disfiguring disease caused by thread-like nematodes. This disease affects the lives of over 120 million people and over one billion people are at risk for infection in endemic regions. Drugs used to treat this disease suffer from toxicity and emerging resistance and new therapies need to be identified. Our laboratory has been studying the filarial parasite, Brugia malayi (B. malayi), one of the causative agents of this disease. The laboratory is focused on the study of critical protein kinase signaling pathways, necessary for parasite protective anti-oxidative responses, as potential therapeutic targets. We have previously determined, …