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A Systematic Review: Longevity And Quality Of Life In Blue Zones, Salmaan Mulla

Theses and Graduate Projects

Ikaria, Loma Linda, Sardinia, Okinawa, and Nikoya. These 5 cities around the world are famed for their increased longevity and quality of life and are termed “Blue Zones”. Blue zones are areas around the world noted for their population of centenarians, overall long life expectancy and high quality of life compared to the average population. Studying these communities offer insights into the commonalities they share to better understand the unique aspects of living a healthy, fulfilling and long life. A comprehensive literature review was conducted using Google Scholar, with the search terms “Ikaria, Greece longevity; Loma Linda, California longevity; Sardinia, …

Steroid Sulfatase In Rodent Tissues And A Cell Line: Characterization, Regulation And Inhibition, Barathi Balasubramonian

Electronic Theses and Dissertations

Inactive sulfated steroids are present in plasma at higher concentrations than unsulfated ones. Steroid sulfatase (STS) is the enzyme that removes the sulfate group from sulfated steroid hormones in local tissues, making them active. High levels of STS are linked to breast and prostate cancers, and low levels of STS cause behavioral disorders and X-linked ichthyosis. STS is present in most tissues, yet its physiological role is uncertain. We sought to characterize STS activity in several rodent model systems in order to learn more about its function. Three different sets of experiments were conducted. First, we examined STS in mouse …

Emotional Response In Working Memory Of Individuals With Latent Toxoplasmosis, Antonella Quimbayo

All HCAS Student Capstones, Theses, and Dissertations

Toxoplasma gondii is a prevalent parasite that infects about 30% of the world population. House cats and other felids serve as definitive hosts and acquire Toxoplasma by consuming infected prey, which are intermediate hosts. This includes rats and avians, but also large mammals such as wolves and deer. Toxoplasma gondii is known to manipulate prey behavior to enhance its transmission. For example, infected rodents demonstrates attraction to and curiosity around cat urine. Wolves infected with Toxoplasma were 11 times more likely to disperse from their pack and start a new pack. The manipulation of intermediate host behavior observed in rodents …

Novel Mechanisms For The Neural Control Of Breathing In Acute Lung Injury, Kajal Kamra

Theses & Dissertations

Acute lung injury (ALI) triggers inflammation that disrupts the normal alveolar-capillary endothelial barrier, impairing gas exchange and causing hypoxemia, which reflexively increases respiration. An elevated respiratory rate (fR) usually develops within hours to days following ALI. This acute respiratory failure disorder affects approximately 200,000 new cases annually in the US and accounts for 10% of ICU admissions, with high morbidity and mortality. The neural mechanisms underlying respiratory dysfunction post-ALI are not fully understood. My doctoral dissertation aimed to investigate the mediators of abnormal ventilation during ALI. Systemic hypoxia stimulates the carotid body (CB) chemoreflex, a crucial protective reflex that maintains …

Elucidating The First Protective Mutation From Inherited Alzheimer’S Disease - Apolipoprotein E3 Christchurch (Apoe3ch) And Insights Into Apoe Ligand-Receptor Interactions, Yun Chen

Arts & Sciences Graduate Student Theses and Dissertations

Alzheimer’s disease (AD) is the major cause of dementia. An inherited form of AD (dominant inherited AD) starts much earlier in life, often in the fourth and fifth decade. A recent case report described an individual who was resistant to dominantly inherited AD caused by a PSEN1-E280A mutation and was a homozygous carrier of the APOE3 Christchurch (APOE3ch) variant. A clear separation between amyloid and tau pathology occurred in this unique AD case. However, whether APOE3ch contributed to the protective effect remains unclear. In this thesis, we generated a humanized APOE3ch knock-in mouse and crossed it to an amyloid-β (Aβ) …

Systemic Activation Of The Axon Degeneration Executioner Sarm1 Triggers Acute Hyperglycemia, Lillian Spatz

Arts & Sciences Graduate Student Theses and Dissertations

Communication between the nervous system and metabolic tissues directs whole body homeostasis. It is well understood that peripheral tissues respond to neural stimulation or inhibition to modulate energy usage and storage, hormone release, and stress pathways, but the neuroendocrine circuits that drive these outcomes remain ill-defined. Our lab identified sterile alpha and TIR motif containing 1 (SARM1) as the central executioner of axon degeneration in injury and disease models. We found that SARM1 is activated by an elevated NMN/NAD+ ratio and that nicotinamide analogs, including 3-AP and vacor, are converted to NMN-like intermediates that directly bind to SARM1, mimicking the …

Discovering And Characterizing Conserved Anthelmintic Targets In The Early Parasitic Stages Of Gastrointestinal Nematodes, Hyeim Jung

Arts & Sciences Graduate Student Theses and Dissertations

Gastrointestinal nematodes (GINs) collectively infect 1.5 billion people, leading to mortality and various forms of morbidity in humans and hindering normal development in children. GINs also diminish the productivity of food animals, which are crucial for economic and nutritional well-being, particularly in developing countries. Currently, the world still primarily relies on only a few drug classes for global de-worming of GINs in humans and animals. However, the high rate of reinfection, disappointing treatment success rates, and increasing drug resistance make GINs one of the most important contributors to promoting and maintaining global poverty. This highlights the urgent need for more …

Modern Building Structures Are A Landscape-Level Driver Of Bat–Human Exposure Risk In Kenya, Tamika Lunn, Reilly T. Jackson, Paul W. Webala, Joseph G. Ogola, Kristian M. Forbes

Biological Sciences Faculty Publications and Presentations

Identifying the locations and drivers of high-risk interfaces between humans and wildlife is crucial for managing zoonotic disease risk. We suggest that continent-wide improvements to residential housing in Africa are inadvertently creating artificial roosting habitat for synanthropic free-tailed bats (family Molossidae), and that improved buildings are a rapidly accelerating exposure interface that needs urgent research attention and investment. Along a residential gradient in rural southern Kenya, we mapped building use by free-tailed bats in 1109 buildings. We show that bats often roost in human-occupied buildings, with almost one-in-ten buildings exhibiting evidence of bat occupation (9.2%) and one-in-13 found to contain …

Molecular Mechanisms Behind Methionine-Derived Glucosinolate Diversity In Brassica Plants, Vivian Kitainda

Arts & Sciences Graduate Student Theses and Dissertations

In Brassica plants, glucosinolates are a diverse class of natural products that serve as plant defense molecules. Their structural diversity comes from the use of amino acids as precursors and elongation of side chains by up to nine carbons, as well as further chemical modifications. Aliphatic methionine-derived glucosinolates are the most abundant form of these compounds. Although both elongation and chemical modification of amino acid sidechains are important for methionine-glucosinolate diversity, its elongation process has not been well described. The chain elongation enzyme methylthioalkylmalate synthase (MAMS) functions as the glucosinolate pathway gatekeeper through its substrate specificity. The x-ray crystal structure …

Balancing Ecology And Practicality To Rank Waterbodies For Preventative Invasive Species Management, Caleb P. Roberts, William E. Grant, Matthew L. Horton, Lindsey A.P. Labrie, Miranda R. Peterson, Jane S. Rogosh, Hsiao-Hsuan Wang

Biological Sciences Faculty Publications and Presentations

• ‘Early detection and rapid response’ (EDRR) is the most successful framework for preventative invasive species management, but prioritizing localized EDRR actions with limited resources is challenging. An approach that ranks individual locations, such as waterbodies, for EDRR by combining an invasive species' establishment risk with the practicality of managing it could help set reasonable priorities.
• Here, we worked with regional practitioners in Arkansas, USA, and the broader Southeastern USA to co-produce a workflow for preventative aquatic invasive species management that (1) estimates establishment risk under current and future climates with a species distribution model, (2) scores waterbodies according to difficulty …

Immunological Feedback Loops Generate Parasite Persistence Thresholds That Explain Variation In Infection Duration, Clayton E. Cressler, Daniel C.G. Metz, David A. Chang Van Oordt, Andrea L. Graham

School of Biological Sciences: Faculty Publications

Infection duration affects individual host fitness and between-host transmission. Whether an infection is cleared or becomes chronic depends on the complex interaction between host immune responses and parasite growth. Empirical and theoretical studies have suggested that there are critical thresholds of parasite dose that can determine clearance versus chronicity, driven by the ability of the parasite to manipulate host immunity. However, the mammalian immune response is characterized by strong positive and negative feedback loops that could generate duration thresholds even in the absence of direct immunomodulation. Here, we derive and analyse a simple model for the interaction between T-cell subpopulations …

The Role Of Southern Red-Backed Voles, Myodes Gapperi, And Peromyscus Mice In The Enzootic Maintenance Of Lyme Disease Spirochetes In North Dakota, Usa, Michael W. Dougherty, Nathan M. Russart, Robert A. Gaultney, Emily Marie Gisi, Haley M. Cooper, Lindsey R. Kallis, Catherine A. Brissette, Jefferson A. Vaughan

Biology Faculty Publications

Lyme disease has expanded into the Great Plains of the USA. To investigate local enzootic transmission, small mammals were trapped in two forested tracts in northeastern North Dakota during 2012 and 2013. Peromyscus mice and southern red-backed voles, Myodes gapperi, comprised over 90% of all mammals captured. One site was dominated by Peromyscus (79% of 100 mammals captured). At the other site, M. gapperi (59% of 107 mammals captured) was more abundant than Peromyscus (36%). Immature stages of two tick species parasitized small mammals: Dermacentor variabilis and Ixodes scapularis. Larval I. scapularis ectoparasitism was significantly higher on Peromyscus (81% …

The Effects Of People, Time Of Day, And Season On Affiliative And Stereotypic Behaviors In Captive Rhinoptera Bonasus And Other Rays Across Two Human-Interactive Enclosures, Wren C. A. Newman

Theses and Dissertations

Cownose rays (Rhinoptera bonasus) are among the most common species found in aquaria, yet the impact of touch enclosures on their welfare remains largely unknown. While research on animal-visitor interactions has produced mixed results for other species, the lack of studies focusing on fish welfare poses a risk of overlooking potential welfare concerns. Therefore, we aimed to establish a baseline for cownose rays regarding stereotypic behaviors, sociality, and activity in captivity, and to examine the influence of human interaction on these behaviors. We collected over 700 hours of footage from two enclosures at Adventure Aquarium in Camden, NJ …

Sex Differences In The Diets Of Forest-Dwelling Baboons (Papio Anubis), Kara Hartunian

Theses and Dissertations

The ability to eat and switch between many food sources is an adaptative feeding strategy utilized for meeting nutritional needs across different environments, seasons, and physiological conditions. Baboons (genus Papio) excel at both dietary flexibility and foraging selectively, and consequently have one of the most widespread geographic distributions in the Order. To further expand to our understanding of baboon feeding ecology, I analyzed foraging activity and the nutritional characteristics of the diets of the relatively understudied rainforest baboons (Papio anubis) living in Kibale National Park, Uganda. I also investigated if there are differences in food type choice …

Znf397 Deficiency Triggers Tet2-Driven Lineage Plasticity And Ar-Targeted Therapy Resistance In Prostate Cancer, Yaru Xu, Yuqiu Yang, Zhaoning Wang, Martin Sjöström, Yuyin Jiang, Yitao Tang, Siyuan Cheng, Su Deng, Choushi Wang, Julisa Gonzalez, Nickolas A Johnson, Xiang Li, Xiaoling Li, Lauren A Metang, Atreyi Mukherji, Quanhui Xu, Carla R Tirado, Garrett Wainwright, Xinzhe Yu, Spencer Barnes, Mia Hofstad, Yu Chen, Hong Zhu, Ariella B Hanker, Ganesh V Raj, Guanghui Zhu, Housheng H He, Zhao Wang, Carlos L Arteaga, Han Liang, Felix Y Feng, Yunguan Wang, Tao Wang, Ping Mu

Faculty, Staff and Students Publications

Cancer cells exhibit phenotypical plasticity and epigenetic reprogramming that allows them to evade lineage-dependent targeted treatments by adopting lineage plasticity. The underlying mechanisms by which cancer cells exploit the epigenetic regulatory machinery to acquire lineage plasticity and therapy resistance remain poorly understood. We identified zinc finger protein 397 (ZNF397) as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage. ZNF397 deficiency facilitates the transition of cancer cell from an AR-driven luminal lineage to a ten-eleven translocation 2 (TET2)-driven lineage plastic state, ultimately promoting resistance to therapies inhibiting AR signaling. Intriguingly, our …

Impact Of Some Parameters On The Survival And Proliferation Of Foodborne Pathogens: Escherichia Coli, Bacillus Subtilis, Staphylococcus Aureus, And Streptococcus Pyogenes, Hassan Agha, Ahmed Bashir Sanaalla, Muhanad Abdullah Salim Abdulsamad, Maroua Abderrahmane Salhi, Ghada Abdu-Razzaq Mohammad, Abdulmutalib Alabeed Alkamil Allaq

AUIQ Complementary Biological System

Food poisoning caused by foodborne microorganisms is a significant public health concern. This study aims to investigate the impact of several nutritional and environmental parameters on the growth of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Streptococc pyogenes in both model systems and food. The specific impact of temperature, pH, and UV light stress on pathogens.Applying physical stress on microorganisms is a standard method to induce cell inactivation and promote food stability.This study found that the optimal temperature for the growth of these pathogens is between 30°C and 50°C. No growth was observed beyond 50°C, indicating temperature sensitivity; low temperatures …

Effect Of Glucocorticoid Blockade On Inflammatory Responses To Acute Sleep Fragmentation In Male Mice, Zim Hasan

Masters Theses & Specialist Projects

The association between sleep and the immune-endocrine system is well recognized, but the nature of that relationship is not well understood. Sleep fragmentation induces a pro-inflammatory response in peripheral tissues and brain, but it also activates the hypothalamic-pituitary-adrenal (HPA) axis, releasing glucocorticoids (GCs) (cortisol in humans and corticosterone in mice). It is unclear whether this rapid release of glucocorticoids acts to potentiate or dampen the inflammatory response in the short term. The purpose of this study was to determine whether blocking or suppressing glucocorticoid activity will affect the inflammatory response from acute sleep fragmentation (ASF). Male C57BL/6J mice were injected …

Contaminant Transport Mechanisms And Source Tracking In Karst Groundwater Systems Under Agricultural Land Use, Shreya Pandey

Masters Theses & Specialist Projects

Karst areas in general face severe groundwater contamination problems that can degrade their water quality. In comparison to other landscapes, groundwater within karst regions is more susceptible to contamination. The situation gets worse when agriculture is practiced in these karst regions, because the nutrients and bacteria like nitrates and E. coli (Escherichia coli) are released into the groundwater systems which contaminate the water quality of those karst groundwater systems. This research aims to study the impacts of these contaminants, specifically nitrates and E. coli., by identifying the contaminant transport mechanisms and source tracking of these contaminants in the groundwater under …

The Evolution Of A Response To Smoke Within The Arthropoda, Adam Miles

Masters Theses & Specialist Projects

Many animals have a survival instinct to flee in response to fire, but do they respond to smoke alone? Many arthropods respond to fire or smoke by moving in the opposite direction (a negative taxis) to obtain shelter. At the species level, taxa that have adapted a behavioral response to fire increase their fitness. This response behavior has been observed in many terrestrial arthropods. Still, the behavior is currently unknown for marine, aquatic, or cave arthropods, which are atypically exposed to smoke or fire. This project assesses how often behavioral adaptation to smoke avoidance may have evolved within Arthropoda. Twenty-two …

Characterizing Oxylipins In Human Milk Extracellular Vesicles, Priscila Herrera

Food Science (MS) Theses

Extracellular vesicles (EVs) are non-replicating lipid bilayer membrane-bound particle formed by a parent cell and secreted into the extracellular environment. EVs in human milk are bioavailable and influence physiologic pathways that may contribute to newborn growth and development. There is interest in the oxylipin content of EVs that may contribute to the bioavailability of EVs. This research aims to characterize the oxylipin content of human milk EVs. Centrifugation and precipitation were used to isolate EVs from human milk (n = 20). The Folch method was used to extract lipids, and liquid chromatography-mass spectrometry (LC– MS) was used to qualify and …

The Role Of Elevation In The Migration, Physiology, And Genomic Diversification Of Birds, Jessie L. Williamson

Biology ETDs

Elevational gradients cause profound eco-climatic variation across short distances. Partial pressure of oxygen (PO₂) is a severe challenge that organisms face at high elevations; due partly to specialization on PO₂, most Neotropical bird species have narrow elevational ranges. However, a small fraction are elevational generalists that span sea level to high elevations, and a subset undertake extreme migratory journeys from low to high elevations biannually. My dissertation combines diverse approaches across levels of biological organization to understand how elevation impacts the ecology, evolution, physiology, and migration of montane birds. I examined ‘extreme’ elevational …

Application Of Criminology In Urban Ecology And Evolution: Routine Activity Theory And Field Equipment Disappearance Dynamics, Ignacy Stadnicki, Michela Corsini, Marta Szulkin

Biology Faculty Publications and Presentations

Research in urban ecology and evolution relies on the use of deployable scientific equipment. If left unattended in the field, scientific equipment may be prone to vandalism and theft, especially in the urban space. Here, we empirically applied a theory derived from the field of criminology, specifically the Routine Activity Theory (RAT) framework, to predict disappearance rates of scientific equipment in an ongoing urban ecology research project. First, we tested a routinely applied method of equipment protection – labelling – and investigated whether equipment disappearance varied with label information content and message tone. Second, we examined whether equipment attributes (price, …

Manipulation Of Cellular Proliferating Cell Nuclear Antigen (Pcna) During Herpes Simplex Virus Type 1 Infection, Jessica Packard, Jill A. Dembowski

Electronic Theses and Dissertations

Herpes simplex virus type 1 (HSV-1) is a double stranded DNA virus that replicates within the nucleus of host cells. Using accelerated native isolation of proteins on nascent DNA (aniPOND), cellular proteins were found to associate with HSV-1 replication forks. One host factor of interest is proliferating cell nuclear antigen (PCNA), the DNA sliding clamp that tethers DNA polymerases and DNA damage response proteins to replicating cellular DNA. Although HSV-1 encodes its own DNA polymerase processivity factor (UL42), PCNA plays a key role in HSV-1 infection. We used commercially available PCNA inhibitors and short hairpin RNAs (shRNA) to investigate how …

Diatom Abundance In The Polar Oceans Is Predicted By Genome Size, Wade R. Roberts, Adam M. Siepielski, Andrew J. Alverson

Biological Sciences Faculty Publications and Presentations

A principal goal in ecology is to identify the determinants of species abundances in nature. Body size has emerged as a fundamental and repeatable predictor of abundance, with smaller organisms occurring in greater numbers than larger ones. A biogeographic component, known as Bergmann’s rule, describes the preponderance, across taxonomic groups, of larger-bodied organisms in colder areas. Although undeniably important, the extent to which body size is the key trait underlying these patterns is unclear. We explored these questions in diatoms, unicellular algae of global importance for their roles in carbon fixation and energy flow through marine food webs. Using a …

Aging Antiviral Immunity In The Central Nervous System: Mechanisms And Implications, Artem D. Arutyunov

Arts & Sciences Graduate Student Theses and Dissertations

The complex interplay between aging, neuroinflammation, and neuroimmune responses has become increasingly evident in recent years. Microglia, the resident immune cells of the central nervous system (CNS), play a pivotal role in maintaining neural homeostasis and responding to neuroinflammatory triggers. This dissertation focuses on the role that aging plays in microglial activation and neuroinflammation, specifically in the context of West Nile Virus (WNV) encephalitis. While the incidence of WNV is consistent across age groups, the risk of West Nile Neuroinvasive Disease (WNND), a serious complication of WNV infection, increases with advanced age. Using mouse models of WNV infection, our group …

Epigenetic Dynamics In Zebrafish Development And Regeneration, Yujie Chen

Arts & Sciences Graduate Student Theses and Dissertations

Understanding the mechanisms that underlie cell fate and status decisions remains a complex task in biological research. Despite sharing identical genetic content, cells can differentiate into diverse types or remain in different statuses based on epigenetic dynamics. Here, I have structured my thesis into two distinct but interconnected parts, each focusing on a specific aspect of epigenetic control during zebrafish development and regeneration. The first part of the thesis focuses on the differentiation of pigment cells from neural crest cells to understand how the same progenitor cell population can bifurcate into two different pigment cell lineages. Through a comprehensive analysis …

Partial Immunity To Mycoplasma Gallisepticum After An Initial Infection Is Long-Lasting And Reduces Transmission In Flocks Of Canaries, Madeline Sudnick

Graduate Theses and Dissertations

Pathology during a second infection can be influenced by immunity and an increase in resistance or tolerance of individuals. Population-level differences in the ability to maintain immunity can lead to contrasting survival outcomes between groups and determine how future outbreaks will spread. However, long term wildlife disease surveillance can be challenging due to lack of infrastructure and host-pathogen specific tests. Calculating disease transmission likelihood requires understanding individual-level heterogeneity in pathology and behavior, and there is limited knowledge in understanding how populations in different stages of an epidemic will transmit disease. In Chapter I, we aim to help fill a gap …

Effects Of Land Cover And Land Change On Monarch Butterfly Presence Across The Conus, Chris R. Burkhart

Graduate Theses and Dissertations

The decline of monarch butterfly populations (Danaus plexippus L.) over the past four decades, particularly in their overwintering habitats, has raised concerns about the species' conservation. Habitat loss and modification, particularly within the contiguous United States, are attributed as one of the key drivers of this decline. However, the specific impact of land use and land cover (LULC) changes on monarch distributions remains inadequately documented. This study integrates monarch observations from multiple sources spanning 2008 to 2019 with LULC data to analyze the relationship between habitat configuration and monarch presence. Using a randomized grid sampling approach, habitat configuration metrics were …

Influence Of Invasive Species, Drought, And Stream Permanence On Stream Crayfish Species With A Focus On The Imperiled Mammoth Spring Crayfish (Faxonius Marchandi), Leah Marie Bayer

Graduate Theses and Dissertations

Freshwater crayfish constitute one of the most-threatened taxonomic groups in North America. Invasion by other crayfish species as well as habitat degradation through alteration of natural flow regime are two of the most prominent threats to crayfish persistence. Additionally, extreme weather events like droughts can amplify threats posed by other stressors on crayfish populations and are expected to increase in frequency, magnitude, and/or duration in the future, which may negatively influence narrow-ranged endemic crayfish populations typical of the Southeast. The Mammoth Spring Crayfish, Faxonius marchandi, is a narrow-ranged endemic that occurs in the Spring River drainage of Arkansas and Missouri …

Challenges Of The Fentanyl Epidemic: Understanding The Crisis, Analyzing The Analogs, And Investigating The Detection Methods, Sophia Hontzas

Graduate Student Dissertations, Theses, Capstones, and Portfolios

The fentanyl epidemic has become a significant public health concern, identified by a rise in overdose deaths pertaining to the use of illicitly manufactured fentanyl (IMF). In 2021 alone, there were 70,601 overdose deaths attributed to fentanyl, emphasizing the severity of the crisis. This paper discusses the challenges posed by fentanyl and its analogs, investigating the origins, market dynamics, biological impacts, and the issues with detection and regulation. While fentanyl was initially created as a potent pain reliever, its misuse has grown due to the presence of new analogs. Case studies provide insight into how fentanyl rose in popularity and …