Digital Commons Network^™

Agricultural Research Magazine August 2013

Agricultural Research Magazine

Table of Contents

4 Prescription for Curing Citrus Greening: Apply Heat and Wait

7 Screening Fresh Oranges With UV: Study Pinpoints New Value of Detection Tactic

8 Breed Matters: Selecting Rams for Rangeland Production

10 Scientific Works of Art Reveal a Hidden World

13 ARS Validates Soil Moisture Data Collected Via Satellite

14 New Strategies To Thwart Pecan Scab

15 Fire Ant Venom Compounds May Be Useful as a Fungicide

16 Possible Links Between Soil Microbial Communities and Stroke Risk

18 Early Weaning: A Good Bet for Beef Producers in Drought-Stricken Areas

20 Fungal Foam Tested Against Avocado Threat

21 …

Big Babies, Big Mammas?: Relationship Of Leatherback Hatchling Size And Mother Size, Shane Morales, Violet Campbell, Kelly Stewart

STAR Program Research Presentations

Individual leatherback hatchlings vary in size when compared to individuals from other nests, as well as individuals from the same nest. It is thought that many factors affect hatchling size but that one of the most influential factors is maternal size. Of all the aspects of a mother which could affect hatchling size, evidence for question concerning influence of mother size is determinable within the field using minimal tools and basic statistical analysis. If a direct correlation exists between mother size and hatchling size then the claim can be made that larger mothers produce larger offspring while smaller mothers produce …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley N. Contreras

STAR Program Research Presentations

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of seagrass and algae to bare rock exposed to crashing waves. Initial field observations of two clades from different locations suggested that behavioral variation may relate to habitat differences among clades. To measure differences in activity, the righting response was timed at both field sites. As a result of behavioral variation observed in the field, more …

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …

Greater Prairie-Chicken Nest Success And Habitat Selection In Southeastern Nebraska, Ty W. Matthews, Andrew J. Tyre, J. Scott Taylor, Jeffrey J. Lusk, Larkin A. Powell

School of Natural Resources: Faculty Publications

Greater prairie-chickens (Tympanuchus cupido pinnatus) are reported to benefit from grasslands created through the Conservation Reserve Program (CRP). Prairie-chicken population size increased noticeably in southeastern Nebraska after >15% of county-level landscapes were converted to CRP grasslands. But, the mechanisms behind the increase in population size are not well understood, and managers and policy makers could benefit from evidence of CRP’s relative contribution to populations of prairie-chickens. Therefore, our objectives were to characterize the relations of vegetation structure and composition with prairie-chicken nest-site selection and nest survival rates at both the macrohabitat (within landscape of study site) and microhabitat …

Leatherback Hatchling Fitness, Violet Campbell, Shane Morales, Kelly Stewart

STAR Program Research Presentations

Leatherback hatchling fitness on land is essential for their success in entering the ocean without being predated. Two ways fitness on land may be measured are by how fast hatchlings crawl as well as how fast their righting responses are when they are flipped onto their carapaces. Hatchlings from certain nests have slower righting response times along with slower crawl times, both of which would increase the odds of predation and hinder the success of hatchlings reaching the ocean.

Hatchling crawling speed was determined by timing 12 randomly selected hatchlings from each nest to crawl a distance of two meters. …

Neurotensin And Cholecystokinin Contract Gallbladder Circular Muscle In Chickens, Teresa F. Degolier, D. R. Brown, Gary E. Duke, M. M. Palmer, J. R. Swenson, Robert E. Carraway

Biological Sciences Faculty Publications

The contractile effects of neurotensin (NT) and cholecystokinin octapeptide (CCK-8) on isolated circular smooth muscle strips of chicken gallbladder were investigated. The NT (0.25-300 nM) produced concentration-dependent contractions on smooth muscle with an EC50 of 8.5 nM (95% confidence limits = 5.3-13.6 nM). In comparison, CCK-8 produced concentration-dependent contractions with an EC50 of 13 nM (95% confidence limits of 9-20 nM). There were no statistical differences in contractile responses when comparing NT and CCK-8 at equimolar concentrations. The NT appears to act directly on smooth muscle tissue in the chicken; the contractile responses were not blocked by 10 μM atropine …

Bios 112l: Introduction To Zoology Lab, Fall 2013, University Of Nebraska-Lincoln, Scott Lyell Gardner, S. Elizabeth Rácz, Altangerel Tsogtsaikhan Dursahinhan

College of Arts and Science: Syllabi for Courses

Syllabus for BIOS 112L Introduction to Zoology Lab, Fall 2013, University of Nebraska-Lincoln.

Lab Instructor, S. (Sarah) Elizabeth Rácz. Teaching Assistants, Altangerel (Auggie) Tsogtsaikhan and Rachel Valenziano.

Goals

1. Give you hands on experience with some of the diverse animal taxa of the planet.

2. Gain a working knowledge of taxonomy, anatomy, and biological vocabulary.

3. Students should gain an understanding of how animals compare and contrast, and how they are adapted to their particular way of life.

4. Get you to think about the natural world.

Material needed for lab

Notebook - Bound, hardback, and unlined pages for sketches …

Examining The Link Between Personality And Laterality In A Feral Guppy Poecilia Reticulata Population, Eleanor Irving, Culum Brown

Sentience Collection

This study examined whether variation in the strength and direction of lateralization in a detour task was linked with variation in three common personality measurements: boldness, activity and sociability, in a population of wild guppies Poecilia reticulata. Additionally, the aim was to determine whether any consistent correlations between these behavioural traits, known as behavioural syndromes, were present in the study population. The results revealed that all three personality traits were highly repeatable over time in both sexes. Evidence of a complex syndrome in the form of a correlation between boldness, sociability and activity was found; however, this relationship was only …

Genome-Wide Prediction Of Age At Puberty And Reproductive Longevity In Sows, Julie Kathleen Tart, Rodger K. Johnson, Justin W. Bundy, N. N. Ferdinand, A. M. Mcknite, Jennifer R. Wood, Phillip S. Miller, M. F. Rothschild, Matthew L. Spangler, Dorian J. Garrick, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Traditional selection for sow reproductive longevity is ineffective due to low heritability and late expression of the trait. Incorporation of DNA markers into selection programs is potentially a more practical approach for improving sow lifetime productivity. Using a resource population of crossbred gilts, we explored pleiotropic sources of variation that influence age at puberty and reproductive longevity. Of the traits recorded before breeding, only age at puberty significantly affected the probability that females would produce a first parity litter. The genetic variance explained by 1-Mb windows of the sow genome, compared across traits, uncovered regions that influence both age at …

Individual Behavioral Variation Of Juvenile Spiny Lobster (Panulirus Argus) Denning Behaviors And The Role It Plays In Shelter Competition During Habitat Loss, Katherine Heldt

All Theses

Variation in juvenile spiny lobster (Panulirus argus) aggressive and gregarious behaviors may play an important role in structuring population level interactions. Since aggressive and gregarious behaviors were not repeatable and were found to be highly correlated with size, these behaviors were found to be largely driven by a combination of behavioral plasticity and ontogeny. Although larger individuals were found to be the most aggressive and least gregarious individuals, often occupying crevice shelters by themselves, they did not exclude smaller, less aggressive lobsters from crevice shelters. Surprisingly, in shelter limited situations, small, less aggressive individuals were more likely to use dens …

Reproductive Isolation And Cryptic Introgression In A Sky Island Enclave Of Appalachian Birds, Gene D. Sattler, Michael J. Braun

Faculty Publications and Presentations

Reproductive isolation is central to the speciation process, and cases where the strength of reproductive isolation varies geographically can inform our understanding of speciation mechanisms. Although generally treated as separate species, Black-capped chickadees (Poecile atricapillus) and Carolina chickadees (P. carolinensis) hybridize and undergo genetic introgression in many areas where they come into contact across the eastern United States and in the northern Appalachian Mountains. The Great Smoky Mountains harbor the last large breeding population of atricapillus in the southern Appalachians, isolated from the species’ main range by nearly 200 km. This population is believed to be reproductively isolated from local …

Use Of Alkaline Treated Crop Residues As Partial Grain Replacements For Finishing Cattle, Adam L. Shreck

Department of Animal Science: Dissertations, Theses, and Student Research

Recently, corn has become expensive and this has led to high feed costs and decreased profitability. High priced corn has allowed for the consideration of other ingredients as replacements to reduce diet cost. In much of the Corn Belt, supplies of wet or modified distillers grains and crop residues are abundant. These ingredients were investigated as corn replacements. Studies were conducted to identify methods for treating crop residues to improve digestibility and value in finishing diets based on corn grain and corn wet distillers grain with solubles (WDGS). Digestibility and value of crop residues were improved by mild chemical treatment. …

Genetic And Environmental Determinants In The Evolution Of Alternative Mating Strategies In The Sailfin Molly, Poecilia Latipinna (Poeciliidae: Poecilia: Mollienesia), Elizabeth Lange

All Theses

Understanding the genetic and environmental factors responsible for differences in size, morphology and behavior can aid in determining how alternative male mating strategies evolve in natural populations. One important environmental factor is nutrition, as it underlies growth of both body size and morphological traits that are linked to alternative mating strategies in many animals. In sailfin mollies, Poecilia latipinna , male size is fixed at maturity, highly variable within populations and correlated with dorsal fin morphology and expression of alternative male mating behaviors. Large males with exaggerated dorsal fins use courtship behavior while small males with reduced dorsal fins use …

The Effects Of Calcium Concentration And Food Levels On Daphnia, Fawn Goodberry

Biology Theses

Many physiological processes of Daphnia are negatively affected at low calcium concentrations. The concentration of calcium within lake water influences how Daphnia populations will survive and reproduce in natural environments. An experiment was designed to test the effects of calcium concentration and food level on the growth and reproduction of a Daphnia hybrid. Daphnia pulex x Daphnia pulicaria were reared in a soft water medium at three calcium concentrations (2.5mg/L, 1.0mg/L, 0.50mg/L) and high and low food levels (5.6 x 10⁵ and 5.6 x 10⁴ cells of the algae Ankistrodesmus sp.) in a 2 x 3 factorial …

Shovelnose Sturgeon Reproductive Ecology In The Lower Platte River, Nebraska, Mathew L. Rugg

School of Natural Resources: Dissertations, Theses, and Student Research

Biodiversity and abundance of freshwater organisms are experiencing drastic declines. Anthropogenic disturbances have altered the natural flow regimes of large rivers, and have led to declines in species that rely on elements of natural flow. Similarly, shovelnose sturgeon distribution has diminished in the last 100 years due to habitat alteration, overharvest, and water contamination. To fully understand the status and viability of a fish population, basic knowledge of a fish’s reproductive strategy is needed. Aspects of reproduction that should be understood to manage for sustainability include maturation, fecundity, and spawning dynamics. There is currently little published information on age/size of …

Population Demographics And Genetics Of Spix's Disk-Winged Bat: Insights Regarding Survival, Mate Choice, Gene Flow And Effective Population Size, Michael Buchalski

Dissertations

Simultaneous study of the demographics and genetics of populations are relatively rare within the literature, despite insights their combined use offers regarding the life history, ecology, and evolution of species. Here I take a comprehensive approach, using capture-recapture data, polymorphic microsatellite markers, and various modeling techniques to examine the demographics and genetics of multiple local populations of Spix’s disk-winged bat (Thyroptera tricolor) in southwestern Costa Rica. T. tricolor is a highly gregarious, neotropical bat species known to form kin-based social groups with high retention of offspring of both sexes. The implications of this highly unusual social structure for …

Experimental Confirmation That Avian Plumage Traits Function As Multiple Status Signals In Winter Contests, Alexis S. Chaine, Allison M. Roth, Daizaburo Shizuka, Bruce E. Lyon

School of Biological Sciences: Faculty Publications

Status signals are thought to reduce costs of overt conflict over resources by advertising social status or an individual’s ability to win contests. While most studies have focused on single badges of status, recent empirical work has shown that multiple status signals may exist. To provide robust evidence for multiple badges of status, an experimental manipulation is required to decouple signals from one another and from other traits linked to fighting ability. Such experimental evidence is lacking for most studies of multiple status signals to date. We previously found that two plumage traits in golden-crowned sparrows, Zonotrichia atricapilla, were correlated …

Oral Presentation Abstracts By Day And Symposium From The 11th International Mammalogical Congress (Belfast, Northern Ireland : August 11-16, 2013), International Mammalogical Congress

University of Nebraska State Museum: Mammalogy Papers

About IMC

IMC returns to Europe after 24 years at a time when IUCN has identified 25% mammal species as at risk. As a venue to share concerns, concepts and techniques among professional mammalogists, IMC has never been more relevant. IMC11 Organising Committee hopes to welcome colleagues with diverse interests in the biology, conservation and management of mammals from throughout the world. IMC11 will encourage active participation in the Congress program by maximising time for spoken papers and posters offered by delegates.

Queen's University Belfast is the venue and are pleased to host the 11th International Mammalogical Congress on behalf …

Coccidia Of Gerbils From Mongolia, Ethan Thomas Jensen

School of Biological Sciences: Dissertations, Theses, and Student Research

In this study, gerbils collected in the Mongolia over the summers of 2009-2012 were examined for coccidia. In total, 171 gerbils of three species from 22 localities were examined for coccidia. Coccidian oocysts were identified from 21 gerbils, but those found in 1 of those gerbils were probably pseudoparasites of the host from which they were recovered. From the remaining 20 gerbils, 7 morphotypes of Eimeria and 1 morphotype of Isospora were identified. Four of the 7 morphotypes of Eimeria were attributed to new species which were described in this study. In addition, 10 previously described species of Eimeria were …

The Role Of Gnrh-Ii And Its Receptor In Testicular Function, Amy T. Desaulniers

Department of Animal Science: Dissertations, Theses, and Student Research

The second mammalian isoform of GnRH (GnRH-II) has been linked to regulation of cell proliferation, feed intake, and the interaction between energy balance and reproductive behavior. In contrast to the native form of GnRH (GnRH-I), GnRH-II is an inefficient modulator of gonadotropin secretion. Unlike many species, a functional receptor (GnRHR-II) specific to this ligand has been discovered in the pig that may be directly involved in testosterone production. Therefore, our objective was to identify the role of GnRH-II and its receptor in testicular function. First, there was 6-fold more GnRHR-II protein in the testis than anterior pituitary gland of boars. …

Development Of Rapid Detection Methods And Novel Control Measures For Salmonella In Poultry, Si Hong Park

Graduate Theses and Dissertations

This dissertation consists of three research parts: 1) development of rapid detection methods for foodborne pathogens; 2) immune response of chicken cells against Salmonella and bacteriophage P22; 3) evaluation of novel control measures for poultry productions. In order to develop rapid and accurate detection methods for foodborne pathogens, two types of PCR assays were utilized. Three foodborne pathogens included Campylobacter, Escherichia coli and Salmonella in watershed were qualitatively and quantitatively detected by multiplex PCR and qPCR (chapter 2). Since Salmonella species are commonly present in poultry and poultry products as well as most popular foodborne pathogen in …

Effects Of Habitat Enrichment On The Stress Level Of The Sit-And-Wait Predator Sand Boa (Gongylophis Colubrinus), Jose Manuel Cantu Martinez

Theses and Dissertations - UTB/UTPA

Conditions in which the animals are housed vary according to animal use. Conditions unfamiliar or unsuited to organisms usually induce stress. Stress is mirrored by a hormone cascade resulting in glucocorticoids. Of the glucocorticoids corticosterone is regarded as the most important. The corticosterone levels of sand boas (Gongylophis colubrinus) were measured under enriched and barren soil and cage features. Enriched soil consisted of enough dirt to allow borrowing. Enriched cage featured consisted of a wooded ball. Barren soil consisted of newspaper as substrate. Barren cage features consisted of plane newspaper. Snakes were subject to treatment for two weeks. No significant …

Characterization Of Host Immune Responses To Eimeria Adenoeides Infection In Turkey Poults, Ujvala Deepthi Gadde

Graduate Theses and Dissertations

Coccidiosis is a common enteric disease of turkeys that is caused by protozoan parasites belonging to the genus Eimeria. There are about seven species of Eimeria that affect turkeys and of these E. adenoeides is the most pathogenic and commonly recognized. Infection with Eimeria is known to induce a long lasting protective immunity in chickens, but nothing is known regarding the acquisition of immunity to Eimeria in turkeys. The experiments reported here were aimed at investigating the biological and cellular immune response to E. adenoeides in turkey poults under different conditions of exposure. In experiment 1 (Chapter II), 20 …

Turpentine Creek: Arkansas' Refuge, Jacob Paul Bain

Graduate Theses and Dissertations

The goal of this Master's thesis film project was twofold. First, I wanted to explore the problem, huge but relatively unknown, of the ownership of exotic pets in the United States of America. Second, in conjunction with that problem, I wanted to see how Turpentine Creek Wildlife Refuge (TCWR) approached it and what, as an organization, they do to help resolve this problem locally and nationally. In turn, the film also provides an in-depth look at a very unique refuge in the state of Arkansas. Using my own equipment, I shot and edited a short-form documentary about TCWR. The film …

The Use Of Non-Traditional Technologies To Improve The Efficiency And Sustainability Of Modern Poultry Production, Christopher Pixley

Graduate Theses and Dissertations

Historically, major driving factors for the animal agriculture industry have been efficiency and profitability. As demand for efficient food production has increased, the industry has focused research efforts on ways to improve the rearing process. Current market demands are requiring the industry to abandon some of the traditional tools it has used to maximize productivity. However, developing alternative technologies are available which may fill the void. Unfortunately, these alternatives are less well-described and the beneficial impacts they can have are not fully understood. As the animal agriculture industry matures it is becoming evident that consumers will continue to demand methods …

Evaluation Of Water Sanitation Options For Poultry Production, Pramir Maharjan

Graduate Theses and Dissertations

An evaluation of poultry farm water supplies was conducted to determine the value and impact of water system sanitation practices in commercial broiler houses on microbial levels. Water line cleaning between flocks using concentrated disinfectant solution before placing chicks reduced biofilms retained in the lines to a safe level. Occasional microbial surges were noticed during different points of flock grow-out period even when daily water sanitation was present indicating water is highly susceptible to microbial contamination. However, the daily water sanitation practice controlled the occasional microbial surges in water from sustaining and kept drinking water to a microbiologically acceptable level. …

Comparison Of Potential Salmonella Portals Of Entry And Tissue Distribution Following Challenge Of Poultry, Gopala Krishna Kallapura Sheshagiri

Graduate Theses and Dissertations

The following studies evaluated our hypothesis that transmission by the fecal-respiratory route may be a viable portal of entry for Salmonella and could explain some clinical impressions of relatively low-dose infectivity under field conditions in relation to the requisite high oral challenge dose that is typically required for infection of poultry through the oral route in laboratory studies. Initial field reports indicating tracheal sampling to be a sensitive tool for monitoring Salmonella infection in commercial flocks, suggested that tracheal contamination could be a good indicator of Salmonella infection under commercial conditions. Further, a usual assumption regarding airborne Salmonella reaching the …

Exploitation Of Channel Catfish In Nebraska Flood-Control Reservoirs, Christopher Lee Wiley

School of Natural Resources: Dissertations, Theses, and Student Research

Channel catfish Ictalurus punctatus, is a popular sportfish in North America, and is the third most-sought fish species in Nebraska. Exploitation rates of channel catfish populations have been estimated to be substantial in states neighboring Nebraska. Despite the popularity of channel catfish, little is known about the exploitation of channel catfish populations in Nebraska. The objectives of this study were to estimate the exploitation rates of channel catfish populations, identify the length bias of angling for channel catfish, and identify the self-imposed length limits for channel catfish at flood-control reservoirs of Nebraska. The software package Program Mark was used to …

Real-Time Bioacoustics Monitoring And Automated Species Identification, T. Mitchell Aide, Carlos Corrada-Bravo, Marconi Campos-Cerqueira, Carlos Milan, Giovany Vega, Rafael Alvarez

Bioacoustics Collection

Traditionally, animal species diversity and abundance is assessed using a variety of methods that are generally costly, limited in space and time, and most importantly, they rarely include a permanent record. Given the urgency of climate change and the loss of habitat, it is vital that we use new technologies to improve and expand global biodiversity monitoring to thousands of sites around the world. In this article, we describe the acoustical component of the Automated Remote Biodiversity Monitoring Network (ARBIMON), a novel combination of hardware and software for automating data acquisition, data management, and species identification based on audio recordings. …