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Notchless Interacts With Multiple Signaling Pathways During Mouse Peri-Implantation Development, Chiao-Ling Lo

Open Access Dissertations

During peri-implantation, the embryo transitions from a suspension environment in the fallopian tubes to an adherent system within the uterus. Successful transition requires maternal and fetal signaling cascades that establish maternal-fetal boundaries. Failure is common, as ~ 25% of all human pregnancies terminate during these steps. A large-scale mutation study in mice produced two mutants (l11Jus1 and l11Jus4) that are excellent models of this transition. l11Jus1 and l11Jus4 contain missense mutations in the Notchless homolog 1 (Drosophila) (Nle1) gene. NLE1 is thought to signal via the canonical NOTCH pathway in vertebrates. Although in invertebrates and lower vertebrates, NOTCH signaling directs …

Store-Operated Calcium Entry And Its Function During Fertilization In Porcine Eggs, Chunmin Wang

Open Access Dissertations

In a variety of mammalian cells, the depletion of intracellular Ca^{2+ stores leads to Ca2+ influx across the plasma membrane though a process known as store-operated Ca2+ entry. It not only plays a critical role in the replenishment of the Ca2+ stores but it is also responsible for the maintenance of long-lasting high intracellular Ca2+ levels and sustaining repetitive Ca2+ oscillations. During fertilization a Ca2+ signal, which can take the form of a single or multiple Ca2+ elevations (depending on the species) is the universal trigger for the egg-to-embryo transition. For sustaining …}

The Genetic Architecture Of Juvenile Migration In Rainbow Trout (Oncorhynchus Mykiss), Benjamin Charles Hecht

Open Access Dissertations

Animal migrations play a critical role in the health and balance of ecological systems and in the evolution and diversification of species, and this is perhaps best displayed amongst salmonid fishes (salmon, trout, and char) who exhibit variation in the propensity to migrate both within and among species. Rainbow and steelhead trout (Oncorhynchus mykiss), members of the salmonid family of fishes, capture this variation throughout their native range. Some populations and ecotypes will remain resident in freshwater habitats throughout their life history; while others have the ability to embark on tremendous marine migrations. Those that migrate undergo a suite of …

Fringed Myotis (Myotis Thysanodes Pahasapensis): Species Conservation Assessment, Melissa J. Panella

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the fringed myotis (Myotis thysanodes pahasapensis) as a Tier I at-risk species. Provided are some general management recommendations regarding the fringed myotis. Conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of the fringed myotis that will aid in the decision-making …

Distribution Extension Of Escherbothrium Molinae Berman And Brooks, 1994 (Cestoda: Tetraphyllidea: Triloculariidae) In Urotrygon Sp. From The Pacific Coast Of Mexico, Francisco Zaragoza-Tapia, Scott Monks, Griselda Pulido-Flores, Juan Violante-González

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cestodes collected from the spiral valves of the stingray Urotrygon sp. from the Pacific coast of Mexico were identified as Escherbothrium molinae Berman and Brooks, 1994. The first report of the species was from the Gulf of Nicoya and the Guanacaste coast, Costa Rica; this work represents the second report of the species since the original description and extends its distribution north to Acapulco, Guerrero, Mexico.

Effects Of Ocean Acidification On The Behavior Of Two Marine Invertebrates: A Study Of Predator-Prey Responses Of The Molluscs Conus Marmoreus And Strombus Luhuanus At Elevated-Co2 Conditions, Jennifer Fields

Independent Study Project (ISP) Collection

Ocean acidification has been affecting the world’s oceans since the introduction of anthropogenic CO2 into the atmosphere during the Industrial Revolution. An increase in CO2 uptake from the atmosphere to the ocean has had a profound impact on not only the water chemistry, but marine organisms as well. Ocean acidification is known to have significant impacts on marine invertebrates in terms of calcification and reproduction; however, effects of increased CO2 on marine invertebrate behavior are vastly unknown. Marine conch gastropods have a modified muscularized foot that allows them to escape quite rapidly when faced with a predator cone shell. Utilizing …

Analysis Of Mitochondrial Dna Structuring Between Colonies Of The World’S Smallest Penguin (Eudyptula Minor) In New South Wales, Australia, Melissa R. Tighe

Independent Study Project (ISP) Collection

The Little Penguin, Eudyptula minor, is a flightless seabird that is endemic to Australia and New Zealand. It can be found nesting on both on and offshore colonies along the coasts of both countries and it is the only penguin currently found breeding on mainland Australia. The IUCN Red List of Threatened Species lists E. minor as “Least Concerned,” but numbers have noticeably dropped in recorded history due to a number of direct and indirect anthropogenic influences. One particular location of decline is Manly, New South Wales that contains the last onshore breeding colony of E. minor in NSW, Australia. …

An Evaluation Of Potential Management Options For The Indo-Pacific Bottlenose Dolphin (Tursiops Aduncus) Population In Kizimkazi, Zanzibar, Rebecca Hamilton

Independent Study Project (ISP) Collection

A short-term study was conducted between November 3rd and November 30th of 2013 in which dolphin area usage was assessed and interviews conducted in order to evaluate which actions should be considered in order to improve dolphin management in Kizimkazi, Zanzibar. GPS coordinates during dolphin sightings were recorded while on tourist and volunteer boat trips to assess dolphin movement and distribution. While on the tourist boats, observations were also made regarding boat operator, tour guide, and tourist knowledge and behavior in relation to tourism interaction guidelines. Interviews were conducted with locals involved in the dolphin tourism sector, tour company representatives, …

Gene Expression Life History Markers In A Hatchery And A Wild Population Of Young-Of-The-Year Oncorhynchus Mykiss, Ian D. F. Garrett

Dissertations and Theses

Life history within a single species can vary significantly. Many of these differences are associated with varying environmental conditions. Understanding what environmental conditions cue alternate life histories within a single species has been researched extensively. In salmonid fishes, more than almost any other group, varying environmental conditions give rise to individuals within species that take markedly different life history trajectories.

Oncorhynchus mykissis a species of salmonid native to the Pacific Northwest region of North America. This species has two life history forms, anadromous and resident. The anadromous form spends a portion of its life in ocean while the resident life …

Reported Incidences Of Parasitic Infections In Marine Mammals From 1892 To 1978, John R. Felix

Zea E-Books Collection

The role of parasites in the lives and deaths of marine mammals has been scrutinized by biologists for decades, but the scientific literature prior to 1978 has been difficult to acquire and time-consuming to search. Now this new and extensive bibliography gives researchers a convenient resource for reviewing the classical literature on parasites of marine mammals so that historical infection prevalence and geographical distribution can be easily and properly assessed. This book contains detailed information about accepted (or suspected) taxonomic synonyms and geographical information about the host and/or parasite, covering the parasite groups Acanthocephala, Acarina, Anoplura, Cestoda, Nematoda, and Trematoda, …

The Tiger Genome And Comparative Analysis With Lion And Snow Leopard Genomes, Yun Sung Cho, Li Hu, Haolong Hou, Hang Lee, Jiaohui Xu, Soowhan Kwon, Sukhun Oh, Hak-Min Kim, Sungwoong Jho, Sangsoo Kim, Young-Ah Shin, Byung Chul Kim, Hyunmin Kim, Chang-Uk Kim, Shu-Jin Luo, Warren E. Johnson, Klaus-Peter Koepfli, A. Schmidt-Kunzel, Jason A. Turner, L. Marker, Cindy K. Harper, Susan M. Miller, Wilhelm Jacobs, Laura D. Bertola, Tae Hyung Kim, Sunghoon Lee, Qian Zhou, Hyun-Ju Jung, Xiao Xu, Priyvrat Gadhvi, Pengwei Xu, Yingqi Xiong, Yadan Luo, Shengkai Pan, Caiyun Gou, Xiuhui Chu, Jilin Zhang, Sanyang Liu, Jing He, Ying Chen, Linfeng Yang, Yulan Yang, Jiaju He, Sha Liu, Junyi Wang, Chul Hong Kim, Hwanjong Kwak, Jong-Soo Kim, Seungwoo Hwang, Junsu Ko, Chang-Bae Kim, Sangtae Kim, Damdin Bayarlkhagva, Woon Kee Paek, Seong-Jin Kim, Stephen J. O'Brien, Jun Wang, Jong Bhak

Biology Faculty Articles

Tigers and their close relatives (Panthera) are some of the world’s most endangered species. Here we report the de novo assembly of an Amur tiger whole-genome sequence as well as the genomic sequences of a white Bengal tiger, African lion, white African lion and snow leopard. Through comparative genetic analyses of these genomes, we find genetic signatures that may reflect molecular adaptations consistent with the big cats’ hypercarnivorous diet and muscle strength. We report a snow leopard-specific genetic determinant in EGLN1 (Met39>Lys39), which is likely to be associated with adaptation to high altitude. We also detect a …

Functional Morphology Of Rat Hands And Feet: Correlation With The Ability To Grip Tree Branches During Locomotion, Jessica E. Fonce, Andrew R. Lammers

Undergraduate Research Posters 2013

Anatomy and function are usually closely related. Since locomotion on tree branches is common among mammals, we expect to find that the anatomy of the hands and feet is well-suited toward gripping narrow, cylindrical, branch-like substrates. We hypothesize that the ability of rats to grip arboreal supports relies on musculature responsible for adducting the first digit (thumb and big toe) and opposing medial-most and lateral-most digits. We dissected the hands and feet of four rat cadavers. There is a substantial muscle that may be responsible for the flexion/adduction of the thumb in the hands. We also found lumbricals, and dorsal …

Status Of Nearshore Finfish Stocks In South-Western Western Australia: Part 1: Australian Herring, Kimberley A. Smith, Joshua Brown, Paul Lewis, Chris Dowling, A Howard, Rod J. Lenanton, Brett Molony

Fisheries Research Reports

The status of the popular nearshore finfish resource in the West Coast Bioregion (WCB) of Western Australia (WA) was largely unknown prior to this study. Previously, declining catches of several nearshore species had highlighted the risk to their sustainability and the need for greater certainty about their status. Recently, the risk further increased due to management changes in the WCB aimed at reducing the catch of demersal scalefish, which are likely to result in a shift in targeting towards nearshore species. This increase in fishing pressure on nearshore species will be on top of any increase due to the continuing …

Fisheries Research Report No. 249 - An Integrated System To Survey Boat-Based Recreational Fishing In Western Australia 2011/12, Karina L. Ryan, Brent S. Wise, Norman G. Hall, K H. Pollock, Elena H. Sulin, Daniel J. Gaughan

Fisheries Research Reports

The Department of Fisheries developed an integrated system involving several survey methods to provide a more robust approach for obtaining annual estimates of recreational catch by boat based fishers at both state-wide and bioregional levels. These surveys, which used the recently implemented Recreational Fishing from Boat Licence (RFBL) as the basis for sampling, were the most comprehensive ever conducted in Western Australia. They not only provided estimates of catch and effort but provided the information for the validation of these estimates by enabling comparisons across the various methods.

Status Of Nearshore Finfish Stocks In South-Western Western Australia: Part 2: Tailor, Kimberley A. Smith, Joshua Brown, Paul D. Lewis, C Dowling, A Howard, R Cj Lenanton, Brett W. Molony

Fisheries Research Reports

The status of the nearshore finfish resource in the West Coast Bioregion (WCB) of Western Australia (WA) was largely unknown prior to this study. Previously, declining catches of several species had highlighted the risk to their sustainability and the need for greater certainty about the status of this popular resource. Recently, the risk substantially increased due to recent changes to the management of demersal scalefish, which are likely to result in a shift in targeting towards nearshore species. This increase in fishing pressure will be on top of any increase due to the continuing human population growth in the WCB.

Status Of Nearshore Finfish Stocks In South-Western Western Australia Part 3: Whiting (Sillaginidae): Nrm Project 09003, Joshua Brown, Christopher Dowling, Alexander Hesp, Kimberley A. Smith, Brett W. Molony

Fisheries Research Reports

The whiting family (Sillaginidae) is common in nearshore waters of south-western Western Australia (WA) where it is highly valued by recreational and commercial fishers. The importance of these species to recreational fishers is reflected in this category of fishes being ranked as the most or 2nd most retained finfish species group by boat- and shore-based fishers in several recreational fishing surveys in WA. Whiting are commercially important in a number of fisheries, with total state catches averaging over 200 tonnes per annum since 1980. The composition of the whiting catch, however, is largely unknown, with a number of whiting species …

Review Of Report On The “Status Of Nearshore Finfish Stocks In South-Western Western Australia: Australian Herring And Tailor”, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Amino Acid Supply And Utilization With Emphasis On Lysine And Impacts On Lactation Responses Of Dairy Cows Fed Rations Containing Distillers’ Dried Grains With Solubles, Henry Paz

Department of Animal Science: Dissertations, Theses, and Student Research

Inclusion of feed coproducts in dairy diets is a common strategy used to reduce the cost of producing milk. The corn ethanol industry produces a coproduct namely distillers dried grains with solubles (DDGS) which could be of high value to the dairy industry because of its high concentration of protein and energy. Under the feeding regimes in the U.S., Lys and Met are recognized as the two most limiting amino acids (AA) for milk protein synthesis. Similar to corn, DDGS is limiting in Lys but not Met. Thus, high inclusions of DDGS have not been favored in the field as …

West Coast Rock Lobster Fishery Ecological Risk Assessment 20 February 2013, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.

Food, Parasites, And Epidemiological Transitions: A Broad Perspective, Karl Reinhard, Luis Fernando Ferreira, Françoise Bouchet, L. Sianto, J. M.F. Dutra, A. Iniguez, Daniela Leles, M. Le Bailley, Martín Horacio Fugassa, Elisa Pucu, Adauto Araújo

Karl Reinhard Publications

Pathoecology provides unique frameworks for understanding disease transmission in ancient populations. Analyses of Old and New World archaeological samples contribute empirically to our understanding of parasite infections. Combining archaeological and anthropological data, we gain insights about health, disease, and the way ancient people lived and interacted with each other and with their environments. Here we present Old and New World parasite evidence, emphasizing how such information reflects the different ways ancient populations exploited diverse environments and became infected with zoonotic parasites. It is clear that the most common intestinal helminthes (worm endoparasites) were already infecting ancient inhabitants of the New …

Bioengineered Lysozyme Reduces Bacterial Burden And Inflammation In A Murine Model Of Mucoid Pseudomonas Aeruginosa Lung Infection, Charlotte C. Teneback, Thomas C. Scanlon, Matthew J. Wargo, Jenna L. Bement, Karl E. Griswold, Laurie W. Leclair

Dartmouth Scholarship

The spread of drug-resistant bacterial pathogens is a growing global concern and has prompted an effort to explore potential adjuvant and alternative therapies derived from nature's repertoire of bactericidal proteins and peptides. In humans, the airway surface liquid layer is a rich source of antibiotics, and lysozyme represents one of the most abundant and effective antimicrobial components of airway secretions. Human lysozyme is active against both Gram-positive and Gram-negative bacteria, ac

Prevalence Of Shiga-Toxin Producing E. Coli On Cattle Farms Located In The Arkansas Delta Region, Monica Nicole Yarbrough

Student Theses and Dissertations

The objective of this study was to determine the prevalence of E. coli STEC on small (30- 40 head) cattle farms in the Arkansas Delta. Samples were collected between 6 September and 25 November 2012 (average air temperature = 16.9oC) from 180 randomly selected beef cows located on 18 farms in five counties (Clay, Craighead, Greene, Poinsett and St. Francis). Samples collected included: 30 rectal swabs from ten cows per farm (3 samples from each cow), three soil samples, three water samples, and when available, three feed samples. The rectal swabs were transferred to 5 ml sterile modified Trypticase Soy …

Modeling The Distribution Of Bobcats And Areas Of Reintroduction For Fisher In The Southern Washington Cascades, Shiloh Michael Halsey

Dissertations and Theses

The fisher (Martes pennanti) is a medium sized member of the mustelid family that once roamed the forests of Washington and whose historic range in the western United States once spread throughout the northern Rocky Mountains, the Cascade and Coast Ranges, and the Sierra Nevada (Carroll, Zielinski, and Noss 1999; Powell 1993, Spencer et al. 2011). Due to pressures from trapping and habitat fragmentation, the abundance of the species in the western United States has decreased dramatically and is thought to be limited to several small, isolated populations. In 2008, fishers were reintroduced to the Olympic Peninsula; however, bobcat (Lynx …

Ccaat/Enhancer-Binding Protein Delta (C/Ebp-Delta) Expression In Antarctic Fishes: Implications For Cell Cycle And Apoptosis, Isaac Martin Sleadd

Dissertations and Theses

Chapter 1: Antarctic fishes are extremely cold adapted. Despite their inability to upregulate heat shock proteins, recent studies have demonstrated a capacity for heat response in these animals. A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivities for hundreds of genes, two of which code for members of the CCAAT/Enhancer-binding protein (C/EBP) family of transcription factors. These molecular switches are best known for their roles in apoptosis, inflammation and cell cycle arrest. This dissertation further elucidates the role of C/EBP-delta in the Antarctic fishes T. bernacchii and Pagothenia borchgrevinki.

Chapter 2: C/EBP-delta is constitutively …

A Note On Pigs’ Knowledge Of Hidden Objects, Christian Nawroth, Mirjam Ebersbach, Eberhard Von Borell

Memory Collection

Object permanence is the notion that objects continue to exist even when they are out of observer’s sight. This ability is adaptive for free ranging animals who have to cope with a dangerous and highly changeable environment and allows them to be aware of predators sneaking in their proximity or to keep track of conspecifics or food sources, even when out of sight. Farm animals might also benefit from object permanence because the ability to follow the trajectory of hidden food or objects may lead to a higher predictability of subjects’ environment, which in turn might affect the level of …

Ecomorphology And Mating Behavior Of Two Species Of Night-Stalking Tiger Beetles, Omus Audouini And O. Dejeanii, Robert Kent Richardson

Dissertations and Theses

Night-stalking tiger beetles (Cicindelinae: Omus) are among the least studied members of the highly diverse Carabid sub-family Cicindelinae, the tiger beetles. Despite populations of Omus being common in the forest floor habitats of the west coast of North America and their conspicuous predatory role within terrestrial arthropod communities, little is known about the biology and ecology of Omus.

Field studies showed that two species of Omus existed in the forested areas of Powell Butte Nature Park, Portland, Oregon, USA: Omus audouiniand O. dejeanii. The co-occurrence of sympatric, and likely syntopic, species allowed for a comparative approach in examining and analyzing …

The Genus Odontophrynus (Anura: Odontophrynidae): A Larval Perspective, Filipe Augusto C. Do Nascimento, Tamí Mott, José A. Langone, Christine A. Davis, Rafael O. De Sá

Biology Faculty Publications

The genus Odontophrynus consists of 11 species of medium-sized frogs distributed across south and east South America. This study examines and describes the chondrocrania and oral cavities of 0. americanus, 0. maisuma, 0. carvalhoi, and 0. cu/tripes, and reviews current knowledge about the larval external morphology of the genus. Twenty-one tadpoles were cleared and double-stained for chondrocranium description and five tadpoles were dissected for analysis in a scanning electron microscope. The presence of a tectum parientale may be considered here as a putative synapomorphy of the genus. The 0. americanus and 0. cu/tripes species groups were partially differentiated by the …

Prescription For Curing Citrus Greening, Dennis O’Brien, Yongping Duan

Agricultural Research Magazine

Huanglongbing (HLB), or citrus greening, is the most serious threat to the Florida citrus industry in its history and is costing millions of dollars each year. Working with the Florida Department of Agriculture and Consumer Services (FDACS), Yongping Duan and his Agricultural Research Service colleagues in Fort Pierce, Florida, have found a formula for curbing HLB, offering the industry a glimmer of hope.

Duan has published details of work showing that heating potted citrus seedlings in greenhouses kills off the HLB bacterium and can rid the seedlings of citrus greening symptoms. Monitoring efforts show that the benefit can last for …

Novel Ways To Combat Plant Pathogens, Deborah Fravel, Gail C. Wisler

Agricultural Research Magazine

Plant diseases must be managed to successfully and reliably produce crops to meet humanity’s growing food, fiber, feed, and fuel needs. Plant disease management relies on many different types of tools—from disease-resistant plant varieties and pesticides to cultural and biological strategies. Agricultural Research Service scientists are seeking new methods of managing plant diseases, more efficient means of using older methods, and combinations of these methods. Two of the oldest ways of reducing plant diseases are crop rotation and organic amendments to soil.

In Orono, Maine, for example, ARS scientists are evaluating a holistic approach to suppressing soilborne pathogens of potato, …

Fire Ant Venom Compounds May Be Useful As A Fungicide, Jan Suszkiw, Jian Chen, Xixuan Jin

Agricultural Research Magazine

Red imported fire ants are named for the firelike burn of their sting. Now, the same venom that packs such a painful wallop may actually do some good for a change.

Studies by scientists at the Agricultural Research Service’s Biological Control of Pests Research Unit in Stoneville, Mississippi, have shown that certain alkaloid compounds in the venom—namely, piperideines and piperidines—can hinder growth of Pythium ultimum, a top crop pathogen worldwide.

Chemical controls, delayed plantings, and crop rotation are among methods now used against P. ultimum, which causes damping-off diseases that decay the seed or seedlings of vegetable, horticultural, …