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Mechanistic Understanding Of Leaky Gut Syndrome In Heat Stressed Broiler Chickens, Jennifer Hoover

Animal Science Undergraduate Honors Theses

Abstract

Broilers, meat-type chickens, are bred for high growth rate and meat yield. These chickens have specific challenges due to heat stress. The adverse effects that impact these chickens have lead us to study the mechanisms involved. The objective of this study is to evaluate the expression of genes involved in intestinal permeability of heat- stressed broiler chickens. All analyses are on previously collected tissues. A total of 600 one day old Cobb 500 male chicks were weighed and randomly assigned to 12 environmental chambers. Ambient temperature in the chambers was gradually decreased from 32˚C on d 1 to 24˚C …

Impact Of Storage Temperature On Viability Of Microbial Inoculum For Estimating In Vitro Equine Digestibility, Delaney Brenne O'Donnell

Graduate Theses/Dissertations

This study evaluated the effect of storage temperature of equine fecal material on the viability of microbial inoculum used for in vitro equine digestibility trials. Fecal material was stored at four storage temperatures of 39°C for 15 minutes (control), 22°C for 6h, 3°C for 6h, and -18°C for 24h. Stored fecal material was used to form microbial inoculum for use in an Ankom Daisy II Incubator. Six different forage samples were digested in the Ankom Daisy II Incubator to evaluate interactions between storage condition of microbial inoculum and chemical composition of digested forage samples. After determination of dry matter digestibility …

Association Of Single Nucleotide Polymorphisms In Bovine Heat Shock Protein 70, Cytochrome P450, Lactate Dehydrogenase, Interleukin 8 Receptor, And Melatonin Receptor 1a With Hoof And Udder Traits In Beef Cows, Maryrose Warren

Animal Science Undergraduate Honors Theses

Hoof and udder conformation are important to the longevity and productivity of beef cattle. Selection for hoof and udder quality reduces the incidence of lameness and udder disease to improve animal welfare. Genetic selection is an effective means of improving traits such as these, especially through the use of single nucleotide polymorphism (SNP) genotyping to identify indicator traits. This study used genotype data and hoof and udder scores from previous research to determine if there is significant effect between polymorphisms of heat shock protein 70 (Hsp70), cytochrome P450 (CYP450), interleukin-8 receptor (IL8), lactate dehydrogenase (LDH), and melatonin receptor 1A (MTNR1A), …

Effect Of Dietary Spirulina Platensis On Stress Levels And Growth Of Female Broiler Chickens, Victoria Tarkington

Animal Science Undergraduate Honors Theses

This study measured the effects of dietary Spirulina platensis supplementation on heterophil to lymphocyte ratio, footpad lesions, and growth performance parameters. One hundred and eighty Ross 708 broiler females were randomly divided evenly into fifteen pens with three dietary treatments for a total of five replications per treatment (twelve birds per replicate) for five weeks. The different treatments were a control diet of 20% crude protein, a diet of 17% crude protein, and a diet of 17% crude protein with 10% S. platensis added to the feed. Body weight, feed intake, and mortality rate was not significantly different between treatments …

Amino Acid Requirements In Horses, Chan Hee Mok, Kristine L. Urschel

Animal and Food Sciences Faculty Publications

Evaluating amino acid requirements, specifically threonine requirements, in horses will enable better feed formulation and result in economic production, improved animal health, and reduced environmental pollution. However, the current knowledge of protein and amino acid requirements in horses is still limited. Because horses have a unique digestive system and consume a variety of feed ingredients, their protein digestibility may be affected than other species by different feed composition, and thus amino acid requirements are susceptible to vary between situations. Therefore, a careful evaluation of amino acid require ments with a proper method is needed for various conditions. This review will …

Impact Of Phosphorus Intake On Beef Heifer Growth Performance And Conception Rates, Hailey Hilfiker

Animal Science Undergraduate Honors Theses

This study aims to examine the effects of phosphorus intake on beef heifer growth performance and conception rates. In Northwest Arkansas, there has been an increase in phosphorus concentrations in soil where livestock manures have been repeatedly applied. Consequently, forages grown on soils high in phosphorus tend to contain high amounts of the mineral itself. This has led many to question whether it is necessary to supplement phosphorus in areas where concentrations may be higher. In this study, crossbred Angus heifers (n = 72), approximately 30 days after weaning, were stratified by body weight (average initial weight 251 ± 3.9 …

Evaluation Of Microbiome On Chicken Necrotic Enteritis And Growth Performance, Mussie Abraha

Graduate Theses and Dissertations

Necrotic enteritis (NE) has re-emerged following restriction of antimicrobial usage and costs $6 billion every year worldwide. The primary objective of the studies was to evaluate prevention and treatment of NE using a microbiota metabolic product, secondary bile acid deoxycholic acid (DCA), in drinking water. Day-old birds were tagged and placed in floor pens. In experiments 1 and 2, the birds were infected with Eimeria maxima (Em) at d 18 and C. perfringens at d 23 and 24 and the birds were euthanized at d 26. In experiment 3, birds were infected with Eimeria at d 20 and C. perfringens …

Evaluation Of Anthelmintic Therapies In A Fall Calving Beef Cowherd, Laine Zammit

Graduate Theses and Dissertations

Internal parasitism inevitability prompts economic loss in beef cattle production by decreasing growth performance and reproductive traits. Today, the most widely used class of anthelmintic used to treat parasitism, is the macrocyclic lactone. Many studies have conflicting results on the efficacy of macrocyclic lactones (ML) efficacy against internal parasitism. The objective of this study was to evaluate the effectiveness of moxidectin and eprinomectin, two of the MLs, on cow performance. Multiparous fall calving, crossbred beef cows (n = 106) were allocated randomly to 1 of 3 anthelmintic treatments: 1) Negative control (CON), in which cows did not receive an anthelmintic, …

Microbiota Characterization Of Poultry Processing Systems And Associated Microbiological Sampling Materials Collected At Commercial Processing Facilities, Jennifer A. Wages

Graduate Theses and Dissertations

The poultry industry and associated regulatory bodies use whole bird carcass (WBC) rinsates to evaluate different stages of broiler processing systems for the prevalence of food-borne pathogens, including Salmonella spp. and Campylobacter spp. Within industry and research groups, the same sample collections are enumerated to determine E. coli, Enterobacteriaceae (EB), and Aerobic Plate Count (APC) microorganisms. Analysis of these indicator microorganisms provides numerical data that can be used to demonstrate the effects of specific process control steps where low occurrences of target pathogens hinder the exclusive use of prevalence data. With the utilization of next generation sequencing (NGS), including analysis …

Development Of Arkansas Net Energy Equation, Katie Hilton

Graduate Theses and Dissertations

The modern broiler is growing at a rapid rate generating tremendous amounts of heat. A sensitive Net Energy (NE) system is needed to measure body heat production (HP) generated primarily by daily maintenance and synthesis and degradation of myofibrillar and sarcoplasmic protein. The first two chapters present evaluation of the classic way to calculate NE versus a new methodology; the Arkansas NE (Ark NE) system, with birds from two genetic lines fed diets with different AA content or different ME content in two different environmental temperatures.

Utilizing together the Net Energy maintenance (NEm), determined from indirect calorimetry, and Net Energy …

Tobacco Enhances Bacterial-Induced Periodontal Bone Loss In Mice., Mina Iskander

Electronic Theses and Dissertations

Background: Tobacco smoking is the leading environmental risk factor for periodontal diseases. Delineation of the mechanisms underlying tobacco-induced or exacerbated periodontitis is hampered by the lack of an appropriate and reliable animal model. Hypothesis: We hypothesized that Porphyromonas-gingivalis-infected, cigarette smoke-exposed mice would represent reproducible models of acute (ligature model) and chronic (oral gavage model) tobacco-enhanced periodontitis that reflect multiple aspects of the disease noted in human smokers. Methods: In a chronic oral gavage disease model, Balb/c mice (6-8 weeks, 4 groups of n = 6 per group) were exposed to smoke produced by a Teague-10 smoking machine from 1R6F research …

Optimal Calving Time For Beef Cows In Southwest Missouri, Briana Rose Verploeg

Graduate Theses/Dissertations

The purpose of this study is to determine if a optimal time of year exists for beef producers to have cows give birth in southwest Missouri for maximal net returns from calf sales and increased cow reproductive performance. To make this determination, data were collected which included year-round forage nutritive value, calf pre-weaning growth, cow energy efficiency and reproductive performance, and income and cost values. Cow and calf field data were gathered for the 2014-2018 production years at Missouri State University’s Leo Journagan Ranch. Monthly forage samples were collected from study cow pastures from 2016 through 2018. Calf, cull cow, …

Laying-Sequence Variation In Yolk Carotenoids Of Eastern Bluebirds, Jessica Barkhouse

Theses and Dissertations

Carotenoids are naturally occurring, fat-soluble pigments that play an important role in the embryonic development of songbirds. Female songbirds use different strategies to allocate these important maternal resources to their eggs. Little research has been done on whether North American songbirds exhibit laying sequence patterns in their allocation of carotenoids. We studied the laying-sequence variation in yolk carotenoids and egg metrics of nine full clutches of Eastern Bluebirds (Sialia sialis) in Columbus, Georgia. I predicted that Eastern Bluebirds would exhibit a brood reduction strategy, with the first-laid eggs containing a higher concentration of carotenoids than the last-laid eggs. …

The Role Of Dopamine In Decision Making Processes In Drosophila Melanogaster, Michelle C. Bowers

Undergraduate Honors Theses

Understanding the neural processes that mediate decision making is a relatively new field of investigation in the scientific community. With the ultimate goal of understanding how humans decide between one path and another, simpler models such as Drosophila Melanogaster, the common fruit fly, are often utilized as a way of determining the neural circuits involved in these decision-making processes. One of the most important decisions flies make is the decision of where to lay their eggs (oviposit). Choosing the proper substrate upon which to lay eggs is a crucial decision that can ultimately impact their fecundity. This paper investigates the …

Evaluation Of Novel Additives And Corn Silage As Natural Alternatives To Antibiotics For The Prevention Of Ruminal Acidosis And Liver Abscesses In Beef Finishing Cattle, Hannah C. Wilson

Department of Animal Science: Dissertations, Theses, and Student Research

Receiving and finishing experiments were conducted to evaluate a non-antibiotic alternative for control of liver abscesses. There was no impact of Ramaekers Immune Primer (RAM; a proprietary blend of vitamins, minerals, prebiotics, and probiotics) treatment on overall performance. Compared to tylosin, RAM was not efficacious in reducing total liver abscesses.

A feedlot study was conducted comparing a natural feed additive (essential oils blend) at varying corn silage inclusion levels on receiving and finishing cattle performance. Inclusion of EO had no effect on performance. Feeding corn silage at greater inclusions decreased daily gain and feed efficiency but increased final body weight …

Organizational Effects Of Exogenous Corticosterone On Vocal Development In A Wild Parrot, Rory Eggleston

Theses and Dissertations

Songbirds learn songs during a plastic phase in vocal development, similar to human vocal babbling. Research has focused on male song learning, a process influenced by gonadal steroids. Like humans, parrots are very plastic vocal learners and both sexes learn vocalizations throughout life. It is unclear how the endocrine system mediates environmental information with developing phenotypes in parrots. Corticosterone (CORT) is a sex-neutral hormone that can affect cognitive development, but its role in vocal development has not been experimentally tested in wild parrots. I quantified vocal babbling from nestling Green-rumped Parrotlets (Forpus passerinus) recorded in Venezuela. Nestlings received oral CORT-oil …

Encephalization In Commensal Raccoons: A Unique Test Of The Cognitive Buffer Hypothesis, Peter M. Anderson

Electronic Theses and Dissertations

This study investigated selective pressures associated with encephalization in mammals and discussed broader implications. Relative brain size as measured by EQ (Encephalization Quotient) was compared between ecological categories. Omnivores had higher average EQ than ecological specialists. Since specialists are disproportionately affected by extinction events, selection for ecological generalism is proposed as encephalization mechanism. This mechanism may reinforce the more widely known Cognitive Buffer Hypothesis (CBH)—the idea that possessing relatively large brains has buffered lineages against environmental change. CBH is tested here by comparing EQs in Procyon lotor (raccoon) in urban and rural environments. CBH predicts that raccoons in the most …

Translational Genomics For Improving Sow Fertility, Hiruni R. Wijesena

Department of Animal Science: Dissertations, Theses, and Student Research

Sow fertility traits, such as litter size and number of lifetime parities produced (reproductive longevity), are economically important. Selection for these traits is difficult because they are lowly heritable, polygenic, sex-limited, and express late in life. Age at puberty is an early indicator of reproductive longevity. Gilts that achieve puberty at an early age have a greater probability to produce more parities over their lifetime. However, measuring age at puberty is time consuming and tedious. Identifying pleiotropic polymorphisms that affect age at puberty and other fertility traits, including reproductive longevity, could help to improve the accuracy of genomic prediction for …

Impact Of Cow Size And Validation Of An Electronic Feeder To Optimize Resources In Beef Production Systems, Robert L. Ziegler

Department of Animal Science: Dissertations, Theses, and Student Research

Optimizing beef production systems is critical in the longevity of an enterprise. In a time of rapid change and innovation, there are increasingly more opportunities to improve efficiency of livestock production by taking advantage of new technologies. Furthermore, production environments vary drastically in all segments of the beef industry, which influence feed resource availability. Therefore, it is critical to realize the cow type that excels in a given production environment according to management objectives. To gain a better understanding of the optimal cow type in the Nebraska Sandhills, a retrospective analysis was conducted to evaluate increasing cow body weight (BW) …

Energy And Amino Acid Metabolism In Lactating Jersey Cows Consuming Feed Byproducts, Kyle Alvin Mclain

Department of Animal Science: Dissertations, Theses, and Student Research

Animal protein byproducts are high bypass proteins commonly used in the dairy industry. These bypass proteins can escape the rumen to supply additional amino acids needed to support milk and protein yield. Two of the more popular animal protein byproducts used in the dairy industry are blood meal and hydrolyzed feather meal. In the first experiment, two flow meters were compared using headbox-style calorimeters. The objectives of the first study were to test mass flow meter (MFM) and volumetric flow meter (VFM) by measuring O₂ consumption and CO₂ production and to illustrate the effects of incomplete gas recovery …

Impact Of Diet And Quality Grade On Meat Quality Characteristics And Their Relationship To Oxidative Stress, Nicolas A. Bland

Department of Animal Science: Dissertations, Theses, and Student Research

Marbling and tenderness in meat are some of the most impactful factors for the consumer eating experience. The cause of the relationship between marbling and tenderness is still unresolved. This study was conducted to evaluate the effects of feeding different types of processed corn, with or without distiller grains on oxidative stress, and how that relates to tenderness and shelf life of beef steaks of differing quality grades. Steers (n = 240) were finished on dry rolled corn (DRC), DRC + 30% dried distillers grains (DDGS), steam flaked corn (SFC), or SFC + 30% DDGS. Cattle were fed 10 …

Usu Equine-Assisted Activities And Therapies Facilities Designed Master Plan, Lindsie C. Smith

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Equine-Assisted Activities and Therapies (EAAT) is recognized as a therapeutic approach for persons with disabilities. The USU Animal, Dairy, and Veterinary Sciences Department provides EAAT instruction and services; however, they do not have the appropriate facilities to model best-practices in the delivery of these services. This design research entailed the development of a phased masterplan to support the instruction and delivery of equine-assisted activities and therapies in an innovative and accessible environment that supports animal-assisted intervention and natural equine behaviors.

The methodology used to approach the master plan design was derived from Norman K. Booth’s (1990) design process, as described …

North Dakota Greater Sage-Grouse (Centrocercus Urophasianus) Recovery Project: Using Translocation To Prevent State-Wide Extirpation And Develop Rangewide Protocols, Kade D. Lazenby

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocurcus urophasianus; hereafter sage-grouse) are the largest grouse species in North America. Sage-grouse occupy 11 western states, extending into North Dakota. North Dakota sage-grouse population is part of the Great Plains Management Zone. Conservation of sage-grouse has been on the forefront of conservation management 1990s. In 2015 the USFWS declared sage-grouse were not warranted for listing based on significant management efforts. Translocations of sage-grouse to prevent populations from extirpation are an example of these efforts.

Translocations have been described as movement and release of animals into a novel environment. There have been more than 7200 sage-grouse …

Effects Of Bovine Maternal Nutrient Restriction On Offspring Microrna And Mrna Expression And Muscle Fiber Type, Nikole E. Ineck

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

For producers in more temperate areas, such as the Intermountain West, poor nutrition during the second trimester of gestation is common due to seasonal changes in forage and nutrient availability. The majority of muscle fibers are formed and adipogenesis is initiated in the second trimester, making it a critical time for skeletal muscle and adipose development in beef cattle. However, the extent to which these changes persist in the offspring postnatally is unknown. In this study, maternal nutrition was restricted during the second trimester in order to analyze the effects of maternal nutrient restriction on offspring skeletal muscle growth. Offspring …

Evaluation Of Maternal Diet And Its Effect On Milk Composition And Piglet Health And Growth Performance, Shana Marie Barnett

Department of Animal Science: Dissertations, Theses, and Student Research

Both graduate research and graduate teaching duties work together to help to develop a graduate student’s skills both in the classroom and on their research experiments. Being both a GTA and GRA allows a student to form more sound hypotheses, connect better with students, and better understand their own research.

During the time as a GTA and GRA four surveys were developed to analyze different groups of students and their learning environment and two animal experiments were conducted to evaluate maternal diet and its effect on milk composition and piglet health and growth performance.

The surveys given to students consisted …

Detection Of Woody Breast Condition In Broiler Carcasses And Product Utilization In Further Processing, Juan Pablo Caldas Cueva

Graduate Theses and Dissertations

Woody Breast (WB) myopathy causes significant economic losses to the global poultry industry and the lack of objective, non-invasive and rapid methods for its detection as well as profitable alternatives for its utilization are contributing factors. This dissertation is divided into two main areas. The first area aimed to determine if there are carcass conformation changes that can be used to identify WB characteristics in commercial broilers using image analysis. In the first experiment, images of broiler carcasses were captured before evisceration to obtain and evaluate carcass measurements from the breast region. Eight image measurements and four ratios were used …

Quantifying Greenhouse Gas Emissions And Ammonia Volatilization From Soil Amended With Excreta From Ruminants Fed Alternative Forage-Based Diets, Samuel Park

Graduate Theses and Dissertations

Forage-based ruminant diets supplemented with condensed tannins or polyphenolic-containing legumes may alter nitrogen (N) metabolism in the animal and reduce gas emissions from soil receiving excreta. The objective of the study was to determine if soil receiving urine or manure from sheep fed forage diets supplemented with condensed tannin or polyphenolic-containing legumes would decrease N gas emissions. Two field trials were conducted: in the first trial, sheep were fed alfalfa (Medicago sativa L.) silage diets supplemented with 0, 9, 18, or 27% sericea lespedeza (Lespedeza cuneate [Dumont de Courset] G. Don) (n = 4). In the second trial sheep were …

Associations Among Beef Cattle Genotypes, Neospora Caninum Infection, And Reproductive Performance, Ryan James Page

Graduate Theses and Dissertations

Reproductive performance is crucial for sustained financial success in the beef cattle industry. This dissertation includes a population study that quantified the incidence of Neospora caninum infections in the central region of the United States and tested its relationship with reproductive performance in beef cattle. Trial one of that study concluded that 6.9% of open, replacement heifers (n = 1306) tested seropositive. The second trial in that project found that 9.6% of the breeding age females (n = 500) tested were seropositive for Neospora caninum; and that state in which the cattle lived and age impacted (P < 0.05) infection rate. Breed composition, number of farm dogs on the ranch, and use of total mixed rations were not associated (P > 0.1) with seropositive …

Effects Of Lasalocid And Energy Supplementation On Forage Intake, Energy Metabolism, And Performance Of Cattle Grazing Wheat Pasture, Autumn Tayler Pickett

Graduate Theses and Dissertations

Cattle grazing wheat pasture have the potential to gain BW exceptionally well, but excessive nitrogen intake results in increased excretion and increased greenhouse gas (GHG) emissions. Supplemental concentrates with the addition of an ionophore given to ruminants grazing wheat is a potential practice for producers to increase nitrogen efficiency while decreasing GHG emissions. Therefore, the objective of this experiment was to quantify the effects of energy (2.95 kg/d) and lasalocid (200 mg/hd/d) supplementation on nutrient intake, energy metabolism, respiratory gas fluxes, and performance of grazing cattle. Methane emissions were not affected (P = 0.58) by treatment, hence methane intensity (P …

A Comparison Of Coyote Diets In Urban And Rural Habitats In The Piedmont Of South Carolina, Bethany Krug

Graduate Theses

With increasing rates of urban expansion, interactions between humans and wildlife become inevitable. These urban environments present novel situations to native species, frequently resulting in their displacement or extirpation. However, some species, often referred to as “urban adapters”, have thrived in these landscapes. Coyotes (Canis latrans) are a prime example of a species that has adapted to exploit urban habitats. Coyotes are omnivores with food choices ranging from small/medium mammals to invertebrates depending on habitat. With their recent range expansion into the Southeast, little is known of their behavioral ecology in the region, especially details relating to their diet. Macroscopic …