Digital Commons Network^™

Bovine Blastocyst-Derived Trophectoderm And Endoderm Cell Cultures: Interferon Tau And Transferrin Expression As Respective In Vitro Markers, Neil C. Talbot, Thomas J. Caperna, J. Lannett Edwards, Wes Garrett, Kevin D. Wells, Alan D. Ealy

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Continuous cultures of bovine trophectoderm (CT-1 and CT- 5) and bovine endoderm (CE-1 and CE-2) were initiated and maintained on STO feeder cells. CT-1 and CT-5 were derived from the culture of intact, 10- to 11-day in vitro-produced blastocysts. CE-1 and CE-2 were derived from the culture of immunodissected inner cell masses of 7- to 8-day in vitro-produced blastocysts. The cultures were routinely passaged by physical dissociation. Although morphologically distinct, the trophectoderm and endoderm both grew as cell sheets of polarized epithelium (dome formations) composed of approximately cuboidal cells. Both cell types, particularly the endoderm, grew on top …

Similarity And Divergence Among Viruses In The Genus Furovirus, Yukio Shirako, Nobuhiro Suzuki, Roy C. French

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nucleotide sequences of RNAs 1 and 2 of a Japanese strain of soil-borne wheat mosaic virus (SBWMV), the type species of the genus Furovirus, and sorghum chlorotic spot virus (SCSV) were determined from cloned cDNA. The relationship among the Japanese and US strains of SBWMV, SCSV, oat golden stripe virus (OGSV), and recently proposed Chinese wheat mosaic and European wheat mosaic viruses (CWMV and EWMV) were examined at the nucleotide and amino acid levels. Pairwise comparisons of genome-encoded proteins among the six viruses showed that the US strains of SBWMV and CWMV were the most closely related pair in RNA …

Biotechnology In Western Australian Agriculture, Agriculture Western Australia

Grain and other field crops published reports

The Government of Western Australia believes that the strategic use of biotechnology and particularly gene technology has the potential to offer Western Australian agriculture significant economic benefits by improving the productivity and quality of products, as well as contributing to the development of sustainable farming systems.

Information contained in these binders is intended to give an overview of Biotechnology in relation to what is occurring in Australia, in Western Australia and specifically, at Agriculture Western Australia (AGWEST).

Biotechnology has been around for twenty-five years or more, but has only recently become a public issue in Australia - particularly in relation …

Herbicide Evaluation In Arkansas Rice, 1998, Ron Talbert, Ford Baldwin, David Gealy, Tomilea Dillon, Lance Schmidt, Eric Scherder, Celeste Wheeler, Leopoldo Estorninos Jr., Jeff Rutledge, Rebecca Chavez

Arkansas Agricultural Experiment Station Research Series

Weed control is economically important for production of rice, a major crop in Arkansas. These findings summarize efforts of the team of Arkansas scientists working on weed control strategies for rice during 1998. Various technologies were evaluated in field studies involving the major weed problems and rice production systems used in the state. Results from these studies will add to the arsenal of weed control options for producers. The preliminary results reported here generally warrant further testing for more advanced findings and for the labeling of new technologies and, finally, are the basis for updating safe, effective, and economical recommendations …

Agriculture Western Australia's Centre For Cropping Systems - Northam, Agriculture Western Australia

Grain and other field crops published reports

Agriculture Western Australia's Centre for Cropping Systems in York Road, Northam, was established as a centre of excellence for research, development and extension into cropping systems.

Its role is to stimulate the contribution of ideas from individuals, groups and organisations with an interest in research, development, and extension relating to innovative, profitable and sustainable crop-based farming systems.

The Centre for Cropping Systems is part of a regional network with strong links to Agriculture Western Australia's Crop Improvement Institute based at South Perth, the Dryland Research Institute at Merredin and the Avondale Research Fann at Beverley. It also links with universities …

B.R. Wells Rice Research Studies 1998, R. J. Norman, T. H. Johnston

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Bulletin 4369 - Ord River Irrigation Area - Kununurra Western Australia, Agriculture Western Australia

Bulletins 4000 -

The Ord River Irrigation Area (ORIA) in the Kimberley Province of Western Australia is the product of a bold and imaginative plan to develop part of the nation's tropical north for intensive agriculture by harnessing the waters of the Ord River. The principal features of the scheme are:

* A major storage reservoir formed by a dam constructed where the Ord River enters the Carr-Boyd Ranges, 160 kilometres south-east of Wyndham.

* A diversion dam, 50 kilometres further downstream, to raise the river level and divert water to be released from the major storage reservoir onto the irrigable lands.

* …

Real-Time Monitoring Of Escherichia Coli O157:H7 Adherence To Beef Carcass Surface Tissues With A Bioluminescent Reporter, Gregory R. Siragusa, Kevin Nawotka, Stanley D. Spilman, Pamela R. Contag, Christopher H. Contag

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. We describe such a method in which we used a bioluminescent reporter strain of Escherichia coli O157:H7 that was constructed by transformation with plasmid pCGLS1, an expression vector that contains a complete bacterial luciferase (lux) operon. Beef carcass surface tissues were inoculated with the bioluminescent strain, and adherent bacteria were visualized in real time by using a sensitive photon-counting camera to obtain in situ …

Horticulture Research Report 1994 - 1998, Frank Wise Institute, Kununurra, Department Of Agriculture, Western Australia, D. Robinson, M. Howes, R. Randell

Horticulture research reports

This report summarises horticultural research, development and extension activities undertaken by staff from the Kununurra District Office during the period 1993 to 1998 and reported annually at the Frank Wise Institute Horticultural Research Review. The range and extent of activities presented and supported by Agriculture Western Australia with industry collaboration indicates the importance of horticulture in the economies of the Kimberley Region and Western Australia. Further support by funding bodies and agribusiness, and collaboration with other research organizations is also acknowledged. Since the early 1980's, horticultural output and value of production from the Ord River Irrigation Area (ORIA) have risen …

Herbicide Evaluation In Arkansas Rice, 1997, Ron Talbert, Ford Baldwin, David Gealy, Eric Webster, Tomilea Dillon, Lance Schmidt, Jeff Rutledge, Celeste Wheller, Leopoldo Estorninos Jr.

Arkansas Agricultural Experiment Station Research Series

Weed control is economically important for production of rice, a major crop in Arkansas. These findings summarize efforts of the team of Arkansas scientists working on weed control strategies for rice during 1997. Various technologies were evaluated in field studies at five locations involving the major weed problems and rice production systems used in the state. Results from these studies will add to the arsenal of weed control options for producers. Highlights include synergists and safeners for herbicides to aid in control of propanil-resistant barnyardgrass; herbicides and flooding techniques for control of red rice and other weeds; and the use …

Intsormil 1999 Annual Report, John M. Yohe, Thomas Crawford Jr., Joan Frederick, Dorothy Stoner

INTSORMIL Impacts and Bulletins

Presently, worldwide, more than 800 million people do not get enough to eat or have access to a balanced diet to be healthy. At the World Food Summit in 1996, the United States of America (USA) and 185 other countries pledged to reduce the number of malnourished people by one half by 2015. It is shocking that about 33% of preschool children in developing countries will be stunted due to malnutrition by the year 2000. The number of stunted children in Africa alone has increased significantly from 35 million in 1980 to 45 million in 1995 and is predicted to …

Notes: Evaluation Of A Filter Bag System For Ndf, Adf, And Ivdmd Forage Analysis, Kenneth P. Vogel, Jeffrey F. Pedersen, Steven D. Masterson, John J. Toy

Department of Agronomy and Horticulture: Faculty Publications

A new method of determining in vitro dry matter digestibility (IVDMD) as recently developed in which the digestion is conducted with the forage samples in filter bags. Our objective was to compare the filter bag and conventional IVDMD analysis methods using smooth bromegrass (Bromus inermis Leyss.), switchgrass (Panicum virgatum L.), and forage sorghum [Sorghum bicolor (L.) Moench] samples. In addition, the filter bag analysis systems for determining non-sequential neutral and acid detergent fiber (NDF and ADF), respectively, were compared with the non-sequential conventional analysis systems. In the filter bag systems, the forage samples are sealed in filter bags and the …

Runoff And Erosion From Interrill Areas As Affected By The Application Of Manure, John E. Gilley, Bahman Eghball, Jurg M. Blumenthal, David D. Baltensperger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study was conducted to measure runoff and erosion from interrill areas as affected by the long-term application of manure and fertilizer to a Tripp sandy loam soil located near Mitchell, Nebraska. Soil which had been removed from the top 0.1 m of the soil profile was placed in a 1 m² soil pan. Rainfall was then applied to the soil pan during initial and wet simulation events. Total runoff was similar on the manure and no-manure treatments. The long-term application of manure (55 years) at a rate of 27 Mg ha^-1 (wet basis) per year did not …

Effect Of Leaf Phosphorus And Potassium Concentration On Chlorophyll Meter Reading In Rice, Shaobing Peng, Arnel L. Sanico, Felipe V. Garcia, Rebecca C. Laza, Romeo M. Visperas, Josue P. Descalsota, Kenneth G. Cassman

Department of Agronomy and Horticulture: Faculty Publications

Chlorophyll meter (SPAD) is a convenient tool to estimate leaf nitrogen (N) concentration of rice plants. There is no information on the effects of leaf phosphorus (P) and potassium (K) concentration on SP AD readings and on the relationship between SPAD values and leaf N concentration in the literature. In 1996 dry season, cv IR72 was grown at the International Rice Research Institute (IRRI) and the Philippine Rice Research Institute (PhilRice) under various N, P and K fertilizer combinations. SPAD measurements were made on the topmost fully expanded leaves at mid-tillering and panicle initiation. The leaves were then detached, dried …

Loci Controlling Resistance To High Plains Virus And Wheat Streak Mosaic Virus In A B73 × Mo17 Population Of Maize, A. Marçon, S. M. Kaeppler, S. G. Jensen, L. Senior, C. Stuber

Department of Agronomy and Horticulture: Faculty Publications

High Plains disease has the potential to cause significant yield loss in susceptible corn (Zea mays L.) and wheat (Triticum aestivum L.) genotypes, especially in the central and western USA. The primary causal agent, High Plains virus (HPV), is vectored by wheat curl mite (WCM; Aceria tossicheila Keifer), which is also the vector of wheat streak mosaic virus (WSMV). In general, the two diseases occur together as a mixed infection in the field. The objective of this research was to characterize the inheritance of HPV and WSMV resistance using B73 (resistant to HPV and WSMV) × Mo17 (moderately …

The Use Of Bauxite Residue As A Soil Amendment In The Peel -Harvey Coastal Plain Catchment - Environmental Monitoring Report August 1999, Mark Rivers

Natural resources published reports

The Peel-Harvey Coastal Plain Catchment is located immediately south of Perth in Western Australia and covers approximately 190,000 ha. It extends from Forrestdale in the north to Harvey in the south, and west from the Darling Scarp to Mandurah on the coast. The boundary of the catchment, as illustrated in Figure 1, represents the area which has been approved by the DEP for the broadscale application of Alkaloam as a soil amendment. (This figure also indicates features and regions of importance in this project).

The region experiences a Mediterranean climate with hot, dry summers and mild, wet winters with most …

Bulletin 4397 - Stone Fruit From Western Australia - At A Glance, Glynn Ward, Geraldine Pasqual, Eleanor Melvin-Carter

Bulletins 4000 -

Western Australia produces a range of horticultural commodities including fruit, vegetables, flowers, nursery products and wine as part of its fast growing $400 million horticultural industry. Natural advantages such as climate and clean environment, soils and water make Western Australia an ideal place to supply a variety of high quality produce to domestic and international markets.

The stone fruit industry in Western Australia is booming with large production increases predicted. Production from well established plum, peach and nectarine orchards in 1996/97 is estimated at $17 million and exports have doubled in the past five years to $10 million in 1998/99. …

Effects On Commercial Broiler Chicks Of Constant Exposure To Ultraviolet Light From Insect Traps, Jerome Hogsette, Henry Wilson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Constant exposure of newly hatched Avian x Avian broilers to ultraviolet light from insect traps for 42 d resulted in no significant differences in mortality, weight gain, feed consumption, or feed conversion. Birds were exposed to greater intensities of ultraviolet light for longer periods than could be expected under commercial conditions. Although house flies are rarely a problem in broiler houses, our results indicate that insect traps with ultraviolet light as an attractant would not be detrimental to production of broilers. The need for additional testing of light traps for nuisance fly control in commercial broiler houses is discussed.

The Reproductive Biology And Effective Pollinators Of The Endangered Beardtongue Penstemon Penlandii (Scrophulariaceae), Vincent J. Tepedino, Sedonia D. Sipes, Terry L. Griswold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Penland's beardtongue, a rare endemic plant of the Colorado Plateau, displays a mixed breeding system. Plants are partially self-compatible but set more fruits when cross-pollinated than when self-pollinated. Fruit production is significantly increased by pollinators. However, in two years of study there was no indication that fruit set was being limited by inadequate pollinator visitation. Pollinator effectiveness was judged by correlating bee behavior at the flowers with analysis of the pollen carried on bee bodies. The most important pollinators were native megachilid bees, particularly in the genus Osmia. The bees that pollinate Penland's beardtongue are essential to its reproduction …

Report Of The Brassica Crops Working Group, Chris Neeser

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Brassicaceae family comprises about 3000 species, the majority of which are found in the Northern Hemisphere. Many common agricultural weeds, such as Brassica nigra (L.) Koch, Brassica rapa L., Cardaria draba (L.) Desv., Raphanus raphanistrum L., and Sinapis arvensis L., belong to this family. The most important crop species from this family are the oilseed Brassicas; Brassica napus L., B rapa L., and B juncea Coss., which are generally referred to as rapeseed, oilseed rape, or canola. Other widely cultivated species in this family are: B. oleracea L. (cabbage, kale, kohlrabi, Brussels sprouts, cauliflower, and broccoli), B. chinensis L. …

Report Of The Grains Working Group (Rice, Sorghum And Wheat), Donna Mitten

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The grains working group identified three crops which have sexually compatible weedy relatives likely to be subject to gene flow in US agricultural systems (Table 1). The ease of cross pollination and the successful production of a fertile hybrid vary with each case. If the selective advantage of an introduced trait is positive, however, introgression of the new trait into an existing weed population is possible. The risk of ecological harm is then dependent upon the habitat of the weed. In the crop-weed complexes considered here, in which the habitat of the weedy relative is limited to agricultural systems, the …

Measurement Of Sorghum Stalk Strength Using The Missouri-Modified Electronic Rind Penetrometer, Jeffrey F. Pedersen, J. J. Toy

Department of Agronomy and Horticulture: Faculty Publications

Resistance to lodging is critical in sorghum [Sorghum bicolor (L.) Moench] grown for grain, yet adequate tools for plant breeders to assess this character at or before anthesis have not been developed. The objective of this study was to evaluate whether an electronic penetrometer previously shown to be an effective tool for measuring stalk strength in maize (Zea mays L.) could be usedd to differentiate among grain sorghum hybrids. Entries in three commercial grain sorghum hybrid trials were evaluated for rind penetrometer resistance (RPR) of the peduncle, the lower stalk anthesis and the lower stalk at maturity. Actual …

The Concept Of Familiarity And Pest Resistant Plants, Karen Hokanson, David Heron, Subhash Gupta, Susan Koehler, Craig Roseland, Shanthu Shantharam, John Turner, James White, Michael Schechtman, Sally Mccammon, Rebecca Bech

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Meetings such as this workshop provide an all too rare opportunity for scientists from different disciplines to share their perspectives on a topic of common interest. In this case we examine the use of pest resistant plants in managed ecosystems. USDA-APHIS has a clear interest in this subject because it is involved in regulating transgenic plants, many of which have been engineered with some sort of pest resistance, within its broad authority to protect plants under the Federal Plant Pest Act and the Plant Quarantine Act. Since 1992, when APHIS received its first request to determine non-regulated status for a …

Report Of The Cucurbit Working Group, James D. Mccreight, Jack Staub

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cucumber (Cucumis sativus L.) originated in India, melon (C. melo L.) and watermelon (Citrullus lanatus) in Africa, and squash, pumpkin, and gourd (Cucurbita spp.) in the Americas. Thus, cucumber, melon, and watermelon (including citron) are relatively recent introductions to the New World. Most domesticated species of Cucurbita were introduced from Mexico, Central America, and South America with the migration of native Americans centuries earlier. Wax gourd (Benincasa hispida (Thunb.) Logr.) is from Southeast Asia. Bottle gourd (Lagenaria siceraria (Molina) Stand.) is of African origin. South Asia is the probable center of …

Analysis Of Human Skin Emanations By Gas Chromatography/Mass Spectrometry. 1. Thermal Desorption Of Attractants For The Yellow Fever Mosquito (Aedes Aegypti) From Handled Glass Beads, Ulrich R. Bernier, Matthew M. Booth, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Handled glass has the ability to collect and concentrate nonaqueous human skin emanations while minimizing the collection of aqueous perspiration. Compounds originating from the skin and collected on glass have previously been found to attract the Aedes aegypti species of mosquito. Therefore, glass beads were used as the medium to collect skin emanations from humans for subsequent chemical analysis. This process consisted of a 5-15-min collection of sample on glass beads, followed by loading the beads into a gas chromatograph (GC) injector insert for subsequent desorption of the collected compounds onto the GC column. After cryofocusing by liquid nitrogen at …

Report Of The Turfgrasses Working Group, Hector Quemada

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Over 30 species of grasses are utilized for turf (Huff 1998), while others are important in agriculture as forage crops. The commercial value of this group of plants makes them attractive for improvement through modern genetic engineering techniques (Johnson and Riordan, in press). Because of the diversity of species and the consequent differences in biology among them, broad generalizations regarding the ecological effects of pest resistance genes introduced into these crops cannot be made. Rather, questions regarding the potential for pest resistance genes must be directed toward specific cases in which the species and the particular introduced gene are known. …

B.R. Wells Rice Research Studies 1997, R. J. Norman, T. H. Johnston

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Comparisons Of Soil Physical Characteristics In Long-Term Tillage Winter Wheat-Fallow Tillage Experiments, L. N. Mielke, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil physical characteristics resulting from tillage of fallow-wheat (Triticurn aestivurn L.) cropping systems were compared for two soils in western Nebraska. The soil physical environment influences the amount of water entering soil and the microenvironment that influences soil biological processes important to plant response. Effects of tillage on physical properties varied with soil type and depth of soil tillage. Generally, the 0-76 mm surface layer has the largest number of physical properties that differ as a result of tillage; however, only a few properties differed at greater depths. The Alliance silt loam (fine silty, mixed, mesic, Aridic Arguistoll) soil …

Potential Of The Entomopathogenic Fungus, Metarhizium Anisopliae (Metsch.) Sorokin For Control Of The Legume Flower Thrips, Megalurothrips Sjostedti (Trybom) On Cowpea In Kenya, S. Ekesi, N. K. Maniania, K. Ampong-Nyarko, I. Onu

All Peer-Reviewed Publications

Field experiments were conducted at Mbita, western Kenya for two seasons to evaluate the potential of the entomopathogenic fungus, Metarhizium anisopliae (Metsh.) Sorokin, for biological control of the legume flower thrips, Megalurothrips sjostedti (Trybom), on cowpea. An ultra-low-volume (ULV) oil/aqueous formulation and a high-volume (HV) aqueous formulation of conidia were applied three times each at two concentrations of 1 x 1011 and 1 x 1013 conidia ha-1. Compared with the untreated controls, both formulations significantly reduced thrips populations and plant damage in both seasons. Adult insects collected from fungal-treated plots and reared in the laboratory showed a high mortality due …

Biodegradation Of Atrazine By Agrobacterium Radiobacter J14a And Use Of This Strain In Bioremediation Of Contaminated Soil, J. K. Struthers, K. Jayachandran, T. B. Moorman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 μg of [¹⁴C-U-ring]atrazine ml^-1 in 72 h with a concurrent increase in the population size from 7.9 x 10⁵ to 5.0 x 10⁷ cells ml^-1. Under these conditions cells mineralized the [ethyl-¹⁴C]atrazine …