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Farmers’ Perceptions Of Importance, Control Methods And Natural Enemies Of Maize Stemborers At The Kenya Coast, M. J. Bonhof, A. Van Huis, F. G. Kiros, N. Dlbogo Jan 2001

Farmers’ Perceptions Of Importance, Control Methods And Natural Enemies Of Maize Stemborers At The Kenya Coast, M. J. Bonhof, A. Van Huis, F. G. Kiros, N. Dlbogo

All Peer-Reviewed Publications

A survey among 240 resource-poor farmers at the Kenya coast indicated that insect pests were considered to be the main production constraint. Over 70% of respondents mentioned stemborers to be the most important insect pest. Unlike the eggs and adults, stemborer larvae and pupae were frequently seen by farmers. Alternative gramineous host plants for pests were common near maize fields and were often not destroyed until after the first rains. Nearly all farmers left dry stems and stubble in the field after harvest. Farmers knew of many methods for controlling stemborers, but only 32 and 56% of farmers in Kilifi …


Distribution And Abundance, In Maize And Sorghum, Of Lepidopteran Stemborers And Associated Indigenous Parasitoids In Zanzibar, E. I. Nlyibigira, Z. S. Abdallah, W. A. Overholt Jan 2001

Distribution And Abundance, In Maize And Sorghum, Of Lepidopteran Stemborers And Associated Indigenous Parasitoids In Zanzibar, E. I. Nlyibigira, Z. S. Abdallah, W. A. Overholt

All Peer-Reviewed Publications

Studies to determine the relative abundance and distribution of stemborer species and their associated parasitoids in maize and sorghum were carried out during the 1995-1996 and 19992000 cropping seasons on Unguja and Pemba, the two main islands of Zanzibar. Three stemborer species were found on both islands in maize and sorghum, with the exotic species Chilo partellns (Swinhoe) (Crambidae) being the most abundant and widely distributed species, accounting for 75.3 % of stemborer attack. It was followed by Sesamia calamistis Hampson (Noctuidae) and C/i. oriclialcociliellus Strand (Crambidae). Stemborer abundance was significantly higher in Pemba (1.47 ± 0.20) than Unguja (0.85 …


Greenhouse Trials Of New Bacillus Thuringiensis Isolates For Control Of Chilo Partellus Larvae In Sorghum, Michael Brownbridge Jan 2001

Greenhouse Trials Of New Bacillus Thuringiensis Isolates For Control Of Chilo Partellus Larvae In Sorghum, Michael Brownbridge

All Peer-Reviewed Publications

Eight new Bacillus thuringiensis Berliner (Bt) isolates originally recovered from different source materials and geographic locations in Kenya were tested against Chilo partellus (Swinhoe) on sorghum grown in a greenhouse. All isolates had been demonstrated previously to be toxic to this pest. Bt strains were cultured in a liquid growth medium and preserved as powders for experimental application. Three-week-old sorghum plants (4-6 leaves) were artificially infested with 20 neonate C. partellus and sprayed 24 h later with Bt suspensions. Non-infested sorghum and infested non-treated sorghum served as the comparative checks within each experimental trial. Plant development and dead-heart formation were …


How To Set Up A Mediterranean Fruit Fly Community Baiting Scheme, Sonya Broughton Nov 2000

How To Set Up A Mediterranean Fruit Fly Community Baiting Scheme, Sonya Broughton

Biosecurity published reports

Second edition.

The Mediterranean fruit fly (Medfly) is one of the most serious economic pests, infesting over 300 fruit and vegetable species worldwide. Medfly is thought to originate from Africa and is a pest in many countries including North America, Central America, South Africa, Europe and Australia. In Australia, Medfly is present only in Western Australia where it is considered to be naturalised. Although Medfly are occasionally detected in eastern Australia, these outbreaks are eradicated at considerable expense by local agricultural departments.

For Western Australia, Medfly poses a serious threat to backyard and commercial fruit production, and restricts access to …


Eucalypt Woodlands A Guide To Management, Liz Brown, Colin Holt Oct 2000

Eucalypt Woodlands A Guide To Management, Liz Brown, Colin Holt

Natural resources published reports

This guide was mainly developed using information gathered from studies in Salmon gum (E. salmonophloia) woodlands. However, the principles in this guide can be broadly applied as it considers the general ecological processes that occur within most woodlands.

If your goal is to restore a woodland to a healthy, functioning unit with a range of original species, this guide provides a way of diagnosing the health of individual woodlands and some of the causes of poor health. It also suggests some relevant management actions.

There is a huge array of different types of Euclaypr Woodlands in the Western Australian Whearbelr, …


Agriculture Western Australia's Great Southern Agricultural Research Institute Katanning, Agriculture Western Australia Sep 2000

Agriculture Western Australia's Great Southern Agricultural Research Institute Katanning, Agriculture Western Australia

Natural resources published reports

Agriculture Western Australia's Great Southern Agricultural Research Institute has been established as a centre of excellence for research, development and extension into sustainable farming systems The. agency has a long association with the town of Katanning dating back to 1925 when the agency provided a general agricultural advisor.

The new Institute is strategically located in the heart of key sheep and cropping production areas with significant growth potential.

Its role is to stimulate the contribution of ideas from individuals, groups and organisations with an interest in research, development and extension relating to innovative, profitable and sustainable animal and cropping based …


B.R. Wells Rice Research Studies 1999, R. J. Norman, C. A. Beyrouty Aug 2000

B.R. Wells Rice Research Studies 1999, R. J. Norman, C. A. Beyrouty

Arkansas Agricultural Experiment Station Research Series

No abstract provided.


Degradation In The Brockman River And Ellen Brook Catchments, Western Australia, Brian Lloyd Jul 2000

Degradation In The Brockman River And Ellen Brook Catchments, Western Australia, Brian Lloyd

Natural resources published reports

An eight day survey of degradation in the Ellen Brook and Brockman River catchments revealed several areas of concern. Key concerns are:

Erosion and sedimentation

Various forms of erosion were observed during the survey that would cumulatively have an effect on the quality of both the Ellen Brook and the Brockman Rivers, and ultimately the Swan River. Erosion resulted from many situations including landholder management, subdivision design and infrastructure maintenance. Raising awareness as to consequences of erosion and sedimentation, along with remedial techniques and best management practices is recommended for the various levels of stakeholders.

Eutrophication

Eutrophication has been established …


Commercial Irrigation And Nutrition Practices For Strawberries In Western Australia, D. Phillips, D. Gatter, T. Calder, D. Pitman Jun 2000

Commercial Irrigation And Nutrition Practices For Strawberries In Western Australia, D. Phillips, D. Gatter, T. Calder, D. Pitman

Horticulture research reports

Irrigation practices employed by five commercial strawberry growers on sandy soils of the Swan Coastal Plain near Perth Western Australia were monitored for two consecutive seasons, 1995 and 1996 during the Spring production period.

Nutritional practices for the same grower group plus one Albany grower were monitored by plant, soil and sap analysis at 14 day intervals throughout the 1996 season.

Irrigation records showed that market quality strawberries could be produced at this time of year (August to December in Perth) on soils with very poor water retention properties with an average crop factor(E Pan) for the season of 0.7 …


Evaluation Of Stomoxys Calcitrans (Diptera: Muscidae) Behavioral Response To Human And Related Odors In A Triple Cage Olfactometer With Insect Traps, Raul A. Alzogaray, David A. Carlson May 2000

Evaluation Of Stomoxys Calcitrans (Diptera: Muscidae) Behavioral Response To Human And Related Odors In A Triple Cage Olfactometer With Insect Traps, Raul A. Alzogaray, David A. Carlson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A triple cage olfactometer provided with insect traps was used for evaluating behavioral responses of Stomoxys calcitrans (L.) females to human skin and breath, CO2, and L-lactic acid analogs. After demonstrating there were no significant differences caused by cage location or time of day, 3 sets of 3 olfactometer tests were performed in a day, every 2 h beginning at 0900 hours. When a human hand was used as attractant, the attraction (expressed as percentage of trapped flies) increased as a function of the time; an inverted U-shaped relationship between attractancy and air speed was observed; and variation …


Wastewater Management Using Hybrid Poplar, Gary A. Kuhn Apr 2000

Wastewater Management Using Hybrid Poplar, Gary A. Kuhn

Agroforestry Notes (USDA-NAC)

Hybrid poplars are rapidly growing trees that are well suited to use agricultural, industrial, and community wastewater. They are being used as an alternative to expensive wastewater treatment systems, and methods which apply wastewater to annual crops or pasture. The trees serve a dual purpose as a nutrient sink for wastewater use and as a means to produce a short-rotation harvested wood product which helps offset the cost of installation and maintenance.

Planning tree wastewater use systems requires both agroforestry and engineering expertise. Omission of either in the planning process can lead to disappointing results and reduced benefits. This Agroforestry …


Waterbreaks: Managed Trees For The Floodplain, Douglas C. Wallace, Wayne A. Geyer, John P. Dwyer Apr 2000

Waterbreaks: Managed Trees For The Floodplain, Douglas C. Wallace, Wayne A. Geyer, John P. Dwyer

Agroforestry Notes (USDA-NAC)

Historically, many of the natural woody ecosystems that were once present in floodplains across the country have been highly altered or removed. Land clearing has resulted in the loss of woody vegetation, even in areas adjacent to streams and rivers. With these alterations, came extensive flood management commitments to compensate for the loss of naturally functioning floodplains and for the protection of towns, roads, and agricultural fields. However, even with the best available flood management techniques, when rivers decide to flood - they will, often with devastating consequences. Strictly from a social and an economic standpoint, allowing the floodplain to …


Effects Of Acid Adaptation Of Escherichia Coli O157:H7 On Efficacy Of Acetic Acid Spray Washes To Decontaminate Beef Carcass Tissue, Elaine Berry, Catherine N. Cutter Apr 2000

Effects Of Acid Adaptation Of Escherichia Coli O157:H7 On Efficacy Of Acetic Acid Spray Washes To Decontaminate Beef Carcass Tissue, Elaine Berry, Catherine N. Cutter

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Exposure to low pH and organic acids in the bovine gastrointestinal tract may result in the induced acid resistance of E. coli O157:H7 and other pathogens that may subsequently contaminate beef carcasses. The effect of acid adaptation of E. coliO157:H7 on the ability of acetic acid spray washing to reduce populations of this organism on beef carcass tissue was examined. Stationary-phase acid resistance and the ability to induce acid tolerance were determined for a collection of E. coliO157:H7 strains by testing the survival of acid-adapted and unadapted cells in HCl-acidified tryptic soy broth (pH 2.5). ThreeE. coli …


Intsormil 2000 Annual Report, John M. Yohe, Thomas Crawford, Jr., Joan Frederick, Dorothy Stoner Jan 2000

Intsormil 2000 Annual Report, John M. Yohe, Thomas Crawford, Jr., Joan Frederick, Dorothy Stoner

INTSORMIL Impacts and Bulletins

Presently, worldwide, more than 800 million people are hungry and over I billion are desperately poor, and food demand is increasing rapidly. The majority of poor live in rural areas in developing countries and agricultural and food systems development is vital to economic growth; improving environmental quality; strengthening nutrition, health and child survival; improving the status of women; and promoting democratization. It is estimated that by 2000, the number of people living in developing countries will grow from 4.9 billion to 6.8 billion people. More than 1.3 billion people today live on less than one dollar per day. It is …


The Wheat Book : Principles And Practice, W K. Anderson, J R. Garlinge Jan 2000

The Wheat Book : Principles And Practice, W K. Anderson, J R. Garlinge

Bulletins 4000 -

Contents : Environment / revised by J. Cramb, J. Courtney and P. Tille - The structure and development of the cereal plant / revised by T.L. Setter and G. Carlton - Germination, vegetative and reproductive growth / revised by T.L. Setter and G. Carlton - Crop water use / D. Tennant - Nutrition / revised by M.D.A. Bolland, R.F. Brennan, J.W. Bowden, M.G. Mason, N.K. Edwards, M.M. Riley and S.W. Gartrell - Wheat in farming systems / revised by B. Bowden, P. Blackwell, P. Carmody, M. Ewing, R. Kingwell, R. L ghman, I. McFarlane, P. Michael, P. Nelson, I. Pritchard, …


Basic Biotechnology, Jennifer Flak, Julie Albrecht Jan 2000

Basic Biotechnology, Jennifer Flak, Julie Albrecht

Plant and Soil Sciences eLibrary

An introduction to traditional cross-breeding and genetic engineering of plants, and to biotechnology.

Objectives - Basic Biotechnology

After completing Nutrition Basic Biotechnology, participants should be able to:

  • Describe one similarity between traditional cross-breeding and genetic engineering of plants.
  • Give two limitations that are encountered with traditional cross-breeding of plants.
  • Explain why genetic engineering is not limited in the same way as traditional cross-breeding.
  • Define biotechnology in broad terms.
  • List three plant applications of biotechnology.
  • List the three areas of nutritional profiles that have been improved with biotechnology.

Modules:


Behavioral Response Of Stomoxys Calcitrans (Diptera: Muscidae) To Conspecific Feces And Feces Extracts, David Carlson, Raul Alzogaray, Jerome Hogsette Jan 2000

Behavioral Response Of Stomoxys Calcitrans (Diptera: Muscidae) To Conspecific Feces And Feces Extracts, David Carlson, Raul Alzogaray, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The attraction response of Stomoxys calcitrans (L.) to its own feces was evaluated in a triple cage olfactometer. Both time- and concentration-response relationships were obtained for female S. calcitrans exposed to cellulose sponges impregnated with fresh fly feces or filter papers treated with chloroform: methanol extracts of fresh fly feces in 6-min tests. Attraction to feces collected on cellulose sponges decreased as the air flow increased. Feces collected on cellulose sponges and held for 28-31 d retained attractive activity. More female flies were attracted than males to feces on sponges or to polar solvent extracts of feces-contaminated cages. The activity …


Yields And Nitrogen Dynamics In A Rice–Wheat System Using Green Manure And Inorganic Fertilizer, Milkha S. Aulakh, Tejinder S. Khera, John W. Doran, Kuldip Singh, Bijay Singh Jan 2000

Yields And Nitrogen Dynamics In A Rice–Wheat System Using Green Manure And Inorganic Fertilizer, Milkha S. Aulakh, Tejinder S. Khera, John W. Doran, Kuldip Singh, Bijay Singh

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Integrating fertilizer N (FN) with legume green manures (GM) can foster sustainable and environmentally sound agricultural systems in subtropical, semiarid soils low in organic matter. A 4-yr replicated field experiment with irrigated rice (Oryza sativa L.) and wheat (Triticum aestivum L.) rotations was conducted on a sandy loam soil in the Punjab of India to evaluate the effects of incorporating cowpea (Vigna unguiculata L.) or sesbania (Sesbania aculeata L.) GM on crop productivity, N-use efficiency, and NO3 leaching. Rice grain yields with pretransplant incorporation of 20 and 40 Mg GM ha-1 (GM20 and …


Scavenging Of H2O2 And Production Of Oxygen By Horseradish Peroxidas, C. Jacyn Baker, Kenneth Deahl, John Domek, Elizabeth W. Orlandi Jan 2000

Scavenging Of H2O2 And Production Of Oxygen By Horseradish Peroxidas, C. Jacyn Baker, Kenneth Deahl, John Domek, Elizabeth W. Orlandi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peroxidases catalyze many reactions, the most common being the utilization of H2O2 to oxidize numerous substrates (peroxidative mode). Peroxidases have also been proposed to produce H2O2 via utilization of NAD(P)H, thus providing oxidant either for the first step of lignification or for the "oxidative burst" associated with plant-pathogen interactions. The current study with horseradish peroxidase characterizes a third type of peroxidase activity that mimics the action of catalase; molecular oxygen is produced at the expense of H2O2 in the absence of other reactants. The oxygen production and H2O2 …


Design And Application Of A Polyclonal Peptide Antiserum For The Universal Detection Of Leptin Protein, Mark P. Richards, Thomas J. Caperna, Theodore H. Elasser, Christopher M. Ashwell, John P. Mcmurtry Jan 2000

Design And Application Of A Polyclonal Peptide Antiserum For The Universal Detection Of Leptin Protein, Mark P. Richards, Thomas J. Caperna, Theodore H. Elasser, Christopher M. Ashwell, John P. Mcmurtry

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

An epitope-specific polyclonal antiserum was produced in rabbits immunized against a synthetic 15 amino acid peptide (QRVTGLDFIPGLHPV) derived from the coding sequence reported for the porcine leptin gene (GenBank Accession No. U59894). This peptide contains a core sequence comprised of eight amino acids (GLDFIPGL) that is totally conserved in all leptin proteins studied to date. Purified recombinant human, mouse, rat, pig, and chicken leptin proteins were separated by polyacrylamide gel electrophoresis (SDS-PAGE) and electro-blotted onto PVDF membranes. Western blots were developed employing the leptin-specific peptide antiserum with an alkaline-phosphatase-conjugated anti-rabbit IgG second antibody chromogenic system. The peptide antiserum was found …


Bulletin No 4315 - Western Australia's Agricultural, Food & Fisheries Industries 1999 - 2000, Besko Trhuij, Francis D'Emden Jan 2000

Bulletin No 4315 - Western Australia's Agricultural, Food & Fisheries Industries 1999 - 2000, Besko Trhuij, Francis D'Emden

Bulletins 4000 -

Fifth Edition (revised and updated) December 2000.

Western Australia’s agriculture and fisheries industries have an international reputation for providing premium quality food and fibre produce to customers around the world.

The State of Western Australia is setting the international quality standard in the production of a vast array of food, fibre and fisheries products.

Agriculture Western Australia has developed the Safe Quality Food System (SQF2000), which not only meets stringent world food standards, but is being adopted in many countries as a unique trademark of quality.

Free of many pests, diseases and pollution found elsewhere, the State offers produce sourced …


Bulletin 4446 - Ord Land And Water Management Plan 2000, Agriculture Western Australia, Community Of Kununurra Jan 2000

Bulletin 4446 - Ord Land And Water Management Plan 2000, Agriculture Western Australia, Community Of Kununurra

Bulletins 4000 -

The wise use of the land and water of the Ord has become an imperative driven by increasing awareness of the impacts of current uses on ground water and the on the quality of the Ord River itself.

We have been lucky as a community that these resources are still in reasonable order and that we have the time to address the resource management challenges associated with our use of them. There is a wide range of land and water management issues to be addressed and all those involved have responsibilities to address some of those issues.


Feeding Fitness And Quality Of Domesticated And Feral Predators: Effects Of Long-Term Rearing On Artificial Diet, Allen Carson Cohen Jan 2000

Feeding Fitness And Quality Of Domesticated And Feral Predators: Effects Of Long-Term Rearing On Artificial Diet, Allen Carson Cohen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Predatory geocorids, Geocoris punctipes Say (Geocoridae: Hemiptera), that had been reared (domesticated) for over 6 years (60 continuous generations) on an artificial diet were compared with feral (F1) counterparts to determine possible domestication-associated losses in predatory capabilities. Using adult female predators provided with either tobacco budworm larvae, Heliothis uirescens F., or pea aphids, Acyrthosiphon pisum Harris, as prey, I measured predator weights, handling time with a single prey, amount extracted, consumption rate, and feeding (gut) capacity. Domesticated females were significantly smaller than ferals, weighing 4.53 mg versus 5.09 mg, respectively. Domestication did not significantly influence handling times, which averaged 131 …


Elongated Chambers For Field Studies Across Atmospheric Co2 Gradients, H. B. Johnson, H. W. Polley, R. P. Whitis Jan 2000

Elongated Chambers For Field Studies Across Atmospheric Co2 Gradients, H. B. Johnson, H. W. Polley, R. P. Whitis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We describe a field facility that controls CO2 along continuous gradients from superambient to subambient concentrations. The facility consists of two transparent, tunnel-shaped chambers, each 1-m wide and tall and 60-m long. Pure CO2 is injected into one chamber during daylight to initiate a superambient CO2 gradient (550–350 µmol mol–1). Ambient air is introduced to the second chamber to initiate a subambient CO2 gradient (350–200 µmol mol–1). CO2 concentrations at night are regulated at 150 µmol mol–1 above daytime values along each gradient. The CO2 gradients are maintained by …


Rising Above The Herd - An Analysis Of The Western Australian Beef & Cattle Industry (Executive Summary Document), Andrew Quinn, Ashley Manners Jan 2000

Rising Above The Herd - An Analysis Of The Western Australian Beef & Cattle Industry (Executive Summary Document), Andrew Quinn, Ashley Manners

Animal production and livestock published reports

This report has been produced at the request of the Agriculture Western Australia, Meat Program Partnership Group. The intention of the report is to set out a number of priority areas for concentrated Agency and industry attention to assist with the development of the Western Australian beef and cattle industry.

  • Global per capita consumption of beef is currently flat to slowly reducing, with total consumption volumes rising in line with population and wealth increases.

  • Global trade in live cattle is experiencing a slow, steady increase in volumes influenced by trade factors encouraging in-country processing and finishing of cattle, and a …


Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass Jan 2000

Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass

Department of Agronomy and Horticulture: Faculty Publications

Improvedmethods for assessing cereal crop feed value are a prerequisite for the genetic improvement of sorghum [Sorghum bicolor (L.) Moench] feed value. Rate of starch digestion is now commonly believed to be the limiting factor in sorghum utilization by cattle (Bos taurus). However, techniques to assess this trait are not useful to sorghum breeders because of high labor inputs, lab error associated with starch measurement, and need for high numbers of replications. The objective of this study was to develop a simple technique capable of identifying differences in digestion between sorghum and corn (Zea mays L.) …


Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins Jan 2000

Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins

Department of Agronomy and Horticulture: Faculty Publications

Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) …


Variation In Boll Weevil (Coleoptera: Curculionidae) Captures In Pheromone Traps Arising From Wind Speed Moderation By Brush Lines, Thomas W. Sappington, Dale W. Spurgeon Jan 2000

Variation In Boll Weevil (Coleoptera: Curculionidae) Captures In Pheromone Traps Arising From Wind Speed Moderation By Brush Lines, Thomas W. Sappington, Dale W. Spurgeon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Paired trap lines of Þve boll weevil pheromone traps each were placed on opposite sides of a brush line at six different sites on a plantation in the Lower Rio Grande Valley of Texas. Temperature, wind speed, and wind direction were monitored with a nearby weather station. We observed a strong negative relationship between mean daily wind speed and total daily capture of boll weevils. About half of the day-to-day variation in weevil captures was explained by wind speed alone. In addition, our data indicate that much of the variation between traps within days may arise from differences in local …


Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl Jan 2000

Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In recent years the accumulation of reactive oxygen species (ROS) has been studied in plant cell suspension systems treated with bacterial pathogens. However, the associated utilization of molecular oxygen has not been well characterized. Using a multi-electrode oxygen analyser, the rates of oxygen consumption by tobacco cells during bacterial interactions were monitored. Heat-killed (HK) bacteria, which initiate an immediate ROS response in plant cells, were used as an elicitor to avoid complications of oxygen consumption by viable bacteria. An increase in oxygen uptake by the tobacco cells occurred within 4 min after addition of HK-bacteria and lasted for about 10 …


Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki Jan 2000

Inhibition Of Cysteine And Aspartyl Proteinases In The Alfalfa Weevil Midgut With Biochemical And Plant-Derived Proteinase Inhibitors, Stephen E. Wilhite, Thomas C. Elden, Joze Brzin, Ann C. Smigocki

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were determined. Hemoglobinolytic activity was highest in the acidic to mildly acidic pH range, but was maximal at pH 3.5. Addition of thiolactivators dithiothreitol (DTT), 2-mercaptoethanol (2-ME), or l-cysteine had little effect on hemoglobin hydrolysis at pH 3.5, but enhanced azocaseinolytic activity two to three-fold at pH 5.0. The broad cysteine PI E-64 reduced azocaseinolytic activity by 64% or 42% at pH 5 in the presence or …