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A Ratiometric Fluorescent Metal Ion Indicator Based On Dansyl Labeled Poly(N-Isopropylacrylamide) Responds To A Quenching Metal Ion, Shawn Burdette, Jie Du, Shaojun Yao, W. Seitz, Nicholas Bencivenga, Justin Massing, Roy Planalp, Randy Jackson, Daniel Kennedy
A Ratiometric Fluorescent Metal Ion Indicator Based On Dansyl Labeled Poly(N-Isopropylacrylamide) Responds To A Quenching Metal Ion, Shawn Burdette, Jie Du, Shaojun Yao, W. Seitz, Nicholas Bencivenga, Justin Massing, Roy Planalp, Randy Jackson, Daniel Kennedy
Shawn C. Burdette
The fluorescence emission of poly(N-isopropylacrylamide) (PNIPAM) covalently tagged with a 5-(dimethylamino)naphthalene-1-sulfonyl (dansyl) fluorophore and an iminodiacetic acid (IDA) chelator changes with temperature and with Cu(II) complexation. Increasing the temperature above the lower critical solution temperature (LCST) causes the polymer to collapse from a coil to a compact globule. This changes the environment experienced by the fluorophore causing a shift in maximum emission wavelength from 546 to 508 nm and an increase in the ratio of emission intensity at 508 nm to emission intensity at 546 nm from 0.70 to almost 1.40. Metal ions can be sensed by working at a …