Open Access. Powered by Scholars. Published by Universities.®
Science and Technology Studies Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Science and Technology Studies
Intermolecular Approach To Metal Ion Indicators Based On Polymer Phase Transitions Coupled To Fluorescence Resonance Energy Transfer, Shawn Burdette, Shaojun Yao, Aaron Jones, Jie Du, Randy Jackson, Justin Massing, Daniel Kennedy, Nicholas Bencivenga, Roy Planalp, W.Rudolf Seitz
Intermolecular Approach To Metal Ion Indicators Based On Polymer Phase Transitions Coupled To Fluorescence Resonance Energy Transfer, Shawn Burdette, Shaojun Yao, Aaron Jones, Jie Du, Randy Jackson, Justin Massing, Daniel Kennedy, Nicholas Bencivenga, Roy Planalp, W.Rudolf Seitz
Shawn C. Burdette
An approach to ratiometric fluorescence detection of quenching metal ions was devised by copolymerizing N-isopropylacrylamide with small percentages of bipyridine and amine monomers. The copolymer was divided into two portions. The amine group on one portion was functionalized with AlexaFluor555 (donor fluorophore) and the other with AlexaFluor647 (acceptor fluorophore). The indicator consists of a mixture of these two portions. Aggregation above the lower critical solution temperature (LCST) of this copolymer brings about a large increase in fluorescence resonance energy transfer (FRET). Addition of Cu(II) and other complexing metal ions to the aggregated copolymer introduces charge onto the backbone, causing the …
A Ratiometric Fluorescent Metal Ion Indicator Based On Dansyl Labeled Poly(N-Isopropylacrylamide) Responds To A Quenching Metal Ion, Shawn Burdette, Jie Du, Shaojun Yao, W. Seitz, Nicholas Bencivenga, Justin Massing, Roy Planalp, Randy Jackson, Daniel Kennedy
A Ratiometric Fluorescent Metal Ion Indicator Based On Dansyl Labeled Poly(N-Isopropylacrylamide) Responds To A Quenching Metal Ion, Shawn Burdette, Jie Du, Shaojun Yao, W. Seitz, Nicholas Bencivenga, Justin Massing, Roy Planalp, Randy Jackson, Daniel Kennedy
Shawn C. Burdette
The fluorescence emission of poly(N-isopropylacrylamide) (PNIPAM) covalently tagged with a 5-(dimethylamino)naphthalene-1-sulfonyl (dansyl) fluorophore and an iminodiacetic acid (IDA) chelator changes with temperature and with Cu(II) complexation. Increasing the temperature above the lower critical solution temperature (LCST) causes the polymer to collapse from a coil to a compact globule. This changes the environment experienced by the fluorophore causing a shift in maximum emission wavelength from 546 to 508 nm and an increase in the ratio of emission intensity at 508 nm to emission intensity at 546 nm from 0.70 to almost 1.40. Metal ions can be sensed by working at a …