Legal Studies Commons^™

Qualitative Analysis Of Luminol Efficacy On Bleach-Cleaned And Paint-Concealed Blood, Adam Wykoff

Themis: Research Journal of Justice Studies and Forensic Science

The presence of blood at a crime scene can provide investigators with a treasure trove of information regarding the nature and circumstances of a particular crime and aid in crime scene reconstruction; however, attempts at concealing blood are common scenarios. The development of chemiluminescent and fluorescent-based presumptive tests, such as the luminol and fluorescein tests, have made it more challenging to definitively remove or mask blood on a surface. The purpose of this experiment was to qualitatively measure the overall efficacy of luminol, concerning its ability to positively detect small bloodstains found on common household floor surfaces (wood, carpet, and …

Postmortem Interval Estimation Based On Eukaryotic Community Associated With Soil Under Decomposing Porcine Remains, Amanda M. Haase

Master of Science in Forensic Science Directed Research Projects

Recent studies have shown that bacteria associated with soils under a carcass change significantly both temporally and spatially. These studies were done in both laboratory and field conditions, but with limited replications. In addition, limited information exists on temporal and spatial changes in eukaryotic community structure associated with soil under decomposing remains. This study was designed to fill in these gaps and expand on previous studies to improve postmortem interval (PMI) estimation techniques as well as to identify cadaver decomposition islands (CDI) based on eukaryotic community structure associated with soil under vertebrate remains. To accomplish this goal, soil samples were …

Development Of A Quantitative Pcr (Qpcr) Based Method For Studying Temporal Dna Degradation In Waterlogged Bone, Isis Thornton

Master of Science in Forensic Science Directed Research Projects

Human activities are often centered around the presence of water, thus it is not surprising that there are many water-related human deaths. Accumulated degree days (ADD), and other aquatic variables may affect DNA retrieval from waterlogged bone. Calcium and collagen in bone can inhibit the PCR necessary to produce an STR profile; the current solution is a time-consuming organic extraction. While there are examples of research on DNA degradation in terrestrial bone over time, there has been little work done on submerged bone samples and they are usually limited to case studies. The major aim of this study was to …

Expanded Testing Of An Integrated Qpcr Mixture Screening Assay Using High Resolution Melt Curve Analysis And Support Vector Machine Modeling, Andrea L. Williams

Master of Science in Forensic Science Directed Research Projects

In the conventional forensic DNA workflow, the number of contributors in a sample is unknown until the final step of STR analysis. We propose a high-resolution melt curve (HRM) mixture screening assay, which uses support vector machine (SVM) modeling of melt morphologies of D5S818 and D18S51 amplicons integrated into a common qPCR-based human DNA quantification kit, to differentiate between single-source samples (and their genotypes) and mixtures at an earlier stage in the DNA workflow (quantification). Previously, using data generated from whole melt curves, 87.5% of single-source samples and 100% of 1:1 mixture samples (2 contributors) classified accurately.

In this study, …

Statistical Evaluation Of Randomly Acquired Characteristics On Outsoles With Implications Regarding Chance Co-Occurrence And Spatial Randomness, Nicole Richetelli

Graduate Theses, Dissertations, and Problem Reports

Footwear evidence holds tremendous forensic value, owing to its ability to formulate linkages between victims, suspects and scenes. Naturally, the strength of these linkages is a function of the perceived clarity, quality and rarity of class, subclass and randomly acquired characteristics (RACs), which are the fundamental outsole features used to formulate source associations. In order to reach a conclusion when performing a footwear comparison, forensic examiners must assign value to the observed similarities and differences that exist between questioned crime scene and test impressions. Embedded within this process is an evaluation of the random association between unrelated shoes as a …

Validation Of Fast Spectrochemical Screening Methods For The Identification Of Counterfeit Pharmaceutical Packaging, Emily Ann Haase

Graduate Theses, Dissertations, and Problem Reports

Counterfeit pharmaceuticals are an actively developing health and economic threat worldwide. Particularly prevalent are counterfeit pharmaceuticals distributed in emerging nations and through internet pharmacies or e-pharmacies. Although technology has been developed that discourages anti-counterfeiting practices (such as optically variable devices, invisible ink, and track-and-trace technology), it remains somewhat novel and expensive to implement on a widespread scale.

In this study, Laser Induced Breakdown Spectroscopy (LIBS) and Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) were proposed as fast and non-invasive tools for the identification of counterfeit pharmaceutical packages. The main objective of this research was to develop and evaluate the capabilities …

The Forensic Analysis Of Adult Temporary Tattoos, Paige Cooper

Student Theses

Adult temporary tattoos have become more popular, yet there is very little research on them, especially regarding their use in forensics. This poses a problem because forensic investigators may not be able to distinguish a temporary tattoo from a real tattoo, possibly hindering their investigation. In this study, various types of adult temporary tattoos were examined and analyzed in attempts to identify and distinguish them, both from each other and from permanent tattoos. Infrared spectroscopy, specifically attenuated total reflection (ATR), proved to be more useful than ultra-violet visible light spectroscopy in terms of analyzing the samples. The natural adult temporary …

The Accumulation Of Wear On Footwear Pattern Analysis, Sarah Pawloski

Themis: Research Journal of Justice Studies and Forensic Science

Wear is defined as the erosion of a shoe’s outsole or the loss of tread pattern, and it happens gradually over time with use. After a shoeprint is collected from a crime scene, it is questioned whether an individualization can be made if an exemplar is created after additional use of the shoe. The shoes of ten volunteers at San Jose State University were analyzed over a period of 40-45 days. Wear and Randomly Acquired Characteristics (RACs) were analyzed over time to determine if there were any observable changes or additions to the already present wear. It was hypothesized that …

Novice Ideas: Handwriting Comparisons Conducted By An Untrained Individual, Maia Lister

Themis: Research Journal of Justice Studies and Forensic Science

Forensic analysis of questioned documents includes chemical analyses of paper and ink as well as handwriting comparisons. Several elements affect handwriting analyses, including the presence of discriminatory factors that can individualize a handwriting sample and whether the handwriting has been disguised. Five handwriting samples were gathered from six individuals comprising of one reference, three natural unknowns, and one disguised sample per person. A novice conducted analyses on every collected sample and conducted comparisons of the reference sample to the unknown and disguised samples in an attempt to correctly source the unknown and disguised samples. The novice showed a high level …

Analysis Of Error Within Forensic Measurements And Photogrammetry Programs, Amy Douglas

Honors Theses

Digital images and photography have been considered commonplace in forensic science since the late 1990’s. Photogrammetry is a tool used in forensic science to measure objects within photographs that contain a scale or programs that are able to measure items in 3D images. Items at crime scenes often need to be measured and forensic scientists may not know what the best option is for their situation. This research will be conducted to show which type of measurement technique is most accurate, calculated against NIST traceable measurements, at various sizes of objects. The measurement techniques that will be tested are standard, …

Determination Of Vehicle Speed From Recorded Video Using Reverse Projection Photogrammetry And File Metadata, Brandon Epstein, Bryce Westlake

Faculty Publications

The prevalence of security and in‐car video has increased the number of motor vehicle accidents captured on digital video. However, inconsistencies in how to accurately determine time and distance for vehicle speed has led to examinations with varying results. A potential solution for calculating time intervals is to use frame timing contained within many digital video file's metadata, recorded with 0.000001 sec precision. This paper examines a fatal motor vehicle accident where frame timing information was used with distance measurements from reverse projection photogrammetry to calculate vehicle speed. A margin of error was then calculated based on the accuracy in …

The Potential Interference Of Body Products And Substrates To The Identification Of Ignitable Liquid Residues On Worn Clothing, Gina Guerrera, Erika Chen, Robert Powers, Brooke Weinger Kammrath

Forensic Science Publications

The question of whether deposits on clothing as well as their chemical composition are being mistaken for ignitable fluids is a concern for forensic analysts. Body products and oil secretions can have similar chemical profiles to ignitable liquid residues (ILRs) as a result of comparable chemical compounds that may be found in both sources. This study investigated whether substrates of unworn and worn clothing, with endogenous body secretions and body products could interfere with ILR analysis. Sample extraction was completed by passive headspace concentration with activated charcoal strips (ACS) and desorption with carbon disulfide followed by analysis with gas chromatography-mass …

Optimizing Methods For Separation Of Adhesive Tape From Fabrics And Obtaining Latent Prints From Adhesive And Non-Adhesive Sides, Elizabeth Vosburgh

Forensic Science Theses

Fingerprinting is a valued part of forensic science analysis. It has been around for decades, and has advanced with the passing of time. There have been numerous studies of the different ways analysts have encountered fingerprints in the field—but none on those deal with the removal of tape from fabric. To investigate this, eight fabric types (a cotton/polyester mix, spandex, denim, jeans, fleece, flannel, polyester, and vinyl), three commercially available tapes (duct tape, black electrical tape, and packaging tape), have been stuck together and separated with four different techniques (manual pulling apart, Un-Du commercial adhesive remover, liquid nitrogen, and a …

Assessing The Reliability Of Physical End Matching And Chemical Comparison Of Pressure Sensitive Tapes, Meghan Nicole Prusinowski

Graduate Theses, Dissertations, and Problem Reports

Pressure sensitive tapes are a common evidence type in a variety of forensic cases, including violent crimes, drug trafficking, and terrorism. Forensic laboratories are often requested to analyze tape samples for composition and potential source identification. Tape samples manufactured from different sources are often distinguishable when using a combination of sensitive analytical techniques. Nonetheless, the interpretation of the data remains challenging due to a lack of standard criteria and guidelines from which examiners can support their conclusions. As a result, there is a need to standardize the protocols of interpretation of physical and chemical comparisons of tape evidence.

The manufacture …

Open-Source Tools For Dense Facial Tissue Depth Mapping (Ftdm) Of Computed Tomography Models, Terrie Simmons-Ehrhardt, Catyana Falsetti, Anthony B. Falsetti, Christopher J. Ehrhardt

Human Biology Open Access Pre-Prints

Computed tomography (CT) scans provide anthropologists with a resource to generate three- dimensional (3D) digital skeletal material to expand quantification methods and build more standardized reference collections. The ability to visualize and manipulate the bone and skin of the face simultaneously in a 3D digital environment introduces a new way for forensic facial approximation practitioners to access and study the face. Craniofacial relationships can be quantified with landmarks or with surface processing software that can quantify the geometric properties of the entire 3D facial surface. This paper describes tools for the generation of dense facial tissue depth maps (FTDMs) using …

Is Forensic Science In Danger Of Extinction?, David San Pietro, Brooke Weinger Kammrath, Peter R. De Forest

Forensic Science Publications

Observations of modern day forensic science has prompted asking the question of whether this field is in danger of extinction. Although there have undoubtedly been meaningful advancements in analytical capabilities, we have overlooked several unintended practical and philosophical consequences. This article addresses three main areas of concern: the declining role of the generalist in an era of increased specialization, the role of education in preparing the next generation of forensic scientists, and the implementation of advanced instrumentation with a focus on statistical significance and field deployable instrumentation.

The Effect Of Fabric Type And Laundering Conditions On The Detection Of Semen Stains, Tyler G. Schlagetter, Claire L. Glynn

Forensic Science Publications

There has been little research into the effect of fabric type and different laundering conditions on the ability to detect semen stains on washed fabrics. This study aimed to investigate three potential factors affecting semen identification on laundered clothing: fabric type, water temperature during washing, and whether the stain was dry at the time of washing. Following laundering, semen stains on four fabric types (cotton, polyester, denim, and wool) were examined and tested with three common methods used to detect semen; screening with an alternate light source, acid phosphatase press test, and histological staining of spermatazoa. It was determined that …

The Advanced Spectroscopic Analysis Of Organic Gunshot Residue And Explosives, Jennifer M. Leonard

Dissertations, Theses, and Capstone Projects

With the prevalence of shooting cases and terrorist attacks/or threats that plague the current state of the criminal justice system, it is of paramount importance to be able to detect, identify and interpret the presence of gunshot residue or explosives material. This concern is seen in law enforcement agencies and the media throughout the United States and abroad.

Currently, the typical method of analyzing gunshot residue in most crime laboratories serves to identify the inorganic constituents of the primer residue, namely lead, barium and antimony. However, it is possible that the organic matter from the propellant could provide different information …

Forensics’ Fight: A Need For Aggressive Strategies Against Confirmation Bias, Madison Mcgowan

Themis: Research Journal of Justice Studies and Forensic Science

In 2009, the National Academy of Sciences produced a lengthy report illuminating significant weaknesses present within the forensic community. One complex fault found in forensics was conformation bias. Since it is within human nature to make decisions based on contextual information, assumptions, and pre-held opinions, confirmation bias is an issue that will continue to persist. Therefore, stronger efforts must be made to recognize and abate the problem of bias within the field of forensics in order to preserve the notion that forensic science exists to serve principles of both truth and justice. Accordingly, this paper argues for the fight against …

Quantitative Analysis Of Opioids And Cannabinoids In Wastewater Samples, Alethea Jacox, Jillian Wetzel, Shu-Yuan Cheng, Marta Concheiro-Guisan

Publications and Research

Wastewater-based epidemiology is an innovative approach that uses the analysis of human excretion products in wastewater to obtain information about exposure to drugs in defined population groups. We developed and validated an analytical method for the simultaneous determination of opioids (morphine, oxycodone, hydrocodone, oxymorphone and hydromorphone), and cannabinoids (Δ⁹-tetrahydrocannabinol, 11-nor-9-carboxy-tetrahydrocannabinol (THCCOOH) and THCCOOH-glucuronide) in raw-influent wastewater samples by ultra-high performance liquid chromatography-tandem mass spectrometry. Method validation included linearity (5–1 000 ng/L for opioids, 10–1 000 ng/L for cannabinoids), imprecision (<21.2%), accuracy (83%–131%), matrix effect (from –35.1% to –14.7%) and extraction efficiency (25%–84%), limit of detection (1–5 ng/L) and quantification (5–10 ng/L) and auto-sampler stability (no loss detected). River and wastewater samples were collected in triplicate from different locations in New York City and stored at −20 °C until analysis. Water from sewage overflow location tested positive for morphine (10.7 ng/L), oxycodone (4.2–23.5 ng/L), oxymorphone (4.8 ng/L) and hydromorphone (4.2 ng/L). Raw influent wastewater samples tested positive for morphine (133.0–258.3 ng/L), oxycodone (31.1–63.6 ng/L), oxymorphone (16.0–56.8 ng/L), hydromorphone (6.8–18.0 ng/L), hydrocodone (4.0–12.8 ng/L) and THCCOOH (168.2–772.0 ng/L). This method is sensitive and specific for opioids and marijuana determination in wastewater samples.

Exploring Myths In Digital Forensics: Separating Science From Ritual, Gary C. Kessler, Gregory H. Carlton

Publications

Digital forensic methodology deviates significantly relative to the methods of other forensic sciences for numerous practical reasons, and it has been largely influenced by factors derived from the inception and evolution of this relatively new and rapidly changing field. Digital forensics methodology was developed more by practitioners in its early days rather than by computer scientists. This led to accepted best practices in the field that may not represent the best or, at least, tested, science. This paper explores some of these differences in the practice and evolution between digital and other forensic sciences, and recommends scientific approaches to apply …

Testing A Novel 3d Printed Radiographic Imaging Device For Use In Forensic Odontology, Tara L. Newcomb, Ann M. Bruhn, Bridget Giles, Hector M. Garcia, M. Arch, Norou Diawara

Dental Hygiene Faculty Publications

There are specific challenges related to forensic dental radiology and difficulties in aligning X-ray equipment to teeth of interest. Researchers used 3D printing to create a new device, the combined holding and aiming device (CHAD), to address the positioning limitations of current dental X-ray devices. Participants (N = 24) used the CHAD, soft dental wax, and a modified external aiming device (MEAD) to determine device preference, radiographer's efficiency, and technique errors. Each participant exposed six X-rays per device for a total of 432 X-rays scored. A significant difference was found at the 0.05 level between the three devices ( …

The Csi Effect: Fact Or Fiction?, Kavita Alejo

Themis: Research Journal of Justice Studies and Forensic Science

The CSI effect has been a subject undergoing intense scrutiny in recent years. With the ever-increasing number of television shows, such as CSI and all of its spinoffs, that poorly represent the field of forensic science, there has also been a growing concern over the effects that media has on the legal system. Prosecutors argue that the CSI effect raises their burden of proof and makes jurors more likely to acquit in cases involving little or no forensic evidence, while defense lawyers claim that jurors are more inclined to wrongfully convict based on their unrealistic perceptions of forensic evidence. This …

A Study Of The Fepac Accredited Graduate Forensic Science Programs' Curricula, Catherine Genice Rushton

Theses, Dissertations and Capstones

The National Institute of Justice (1999) and the National Academy of Sciences (2009) recommended that forensic science training shift from on-the-job training to formal education. However, the reports cited inconsistencies in the curricula of the forensic science degree programs as an impediment to this. The Forensic Science Education Programs Accreditation Commission (FEPAC) Standards were created to address this issue; however, no studies have been conducted to determine how the accreditation standards have been implemented by the FEPAC accredited graduate programs. This study evaluated the self-study responses (n=11) and website information (n=17) specific to FEPAC’s Graduate Curriculum Standards to determine how …

A Descriptive Analysis Of The Appropriate Use Of Cognitive Bias Terminology In Forensic Science Literature, Courtney A. Winters, Evelyn M. Buday, Trevor I. Stamper

The Summer Undergraduate Research Fellowship (SURF) Symposium

Cognitive bias occurs without a person’s awareness and can affect decision-making abilities. In forensic science, bias can be especially detrimental to making accurate decisions about the evidence in a criminal investigation. There are many academic studies in identifying, describing, and suggesting ways to mitigate cognitive biases in forensic science. Many authors will give a known cognitive science concept a new name or create their own bias. This is a problem in the literature because nobody knows for sure how many published studies are referring to or testing the same phenomena since authors are using different definitions or terminology to describe …

Reducing Contamination In Forensic Science, Carly Balk

Themis: Research Journal of Justice Studies and Forensic Science

The sensitivity of modern forensic techniques has drastically increased, with sensitive technology detecting even the smallest traces of DNA evidence left behind. This has made it possible to detect DNA profiles deposited through contamination. When DNA contamination occurs in forensic science, it has the potential to change the outcome of a criminal investigation and may have significant social and financial repercussions. A compilation of global research shows that DNA evidence transfer can occur during forensic product manufacturing, the fingerprinting process, or even autopsy and crime lab examinations. These vital areas of the forensic investigation are vulnerable to contamination, and national …

The "Csi Effect" And Its Potential Impact On Juror Decisions, John Alldredge

Themis: Research Journal of Justice Studies and Forensic Science

The “CSI Effect” was first described in the media as a phenomenon resulting from viewing forensic and crime based television shows. This effect influences jurors to have unrealistic expectations of forensic science during a criminal trial and affect jurors’ decisions in the conviction or acquittal process. Research has shown the “CSI Effect” has a possible pro-defense bias, in that jurors are less likely to convict without the presence of some sort of forensic evidence. Some studies show actors in the criminal justice system are changing their tactics, as if this effect has a significant influence, causing them to request unnecessary …

Quantitative Assessment Of The Effects Of Microbial Degradation Of A Simple Hydrocarbon Mixture, Jessica Kindell

Electronic Theses and Dissertations

Ignitable liquids consist of either a single organic compound or a complex organic mixture. In regards to fire debris analysis, the analyst is responsible for determining if an ignitable liquid residue is present. However, when extracted from soil-containing fire debris evidence, chemical degradation from microorganisms is observed to result in the loss of compounds based on chemical structure. It can also happen when the evidence container is stored at room temperature before analysis. This can present a challenge to the fire debris analyst when identifying and classifying the ignitable liquid residue based on the criteria established by standard test methods. …

Comparing Wearer Dna Sample Collection Methods For The Recovery Of Single Source Profiles, Corissa J. Harris, Amanda J. Cardenas, Steven B. Lee, Brooke Barloewen

Themis: Research Journal of Justice Studies and Forensic Science

Wearer DNA is the deposit of epithelial cells on clothing worn by an individual. Detection of the last individual to handle or wear an item is often an important and desirable determination in forensic science. The most commonly used collection methods for wearer DNA include swabbing and scraping. These often result in mixture profiles. Recently, adhesives have been introduced as a possible reliable method for the collection of biological evidence. The goal of the research was to compare the current collection methods of swabbing and scraping with a gel film called Gel-Pak ‘0’ which shares similar properties with adhesives. Gel-Pak …

Confirmation Bias: The Pitfall Of Forensic Science, Scott Moser

Themis: Research Journal of Justice Studies and Forensic Science

As it stands, forensic science and its practitioners are held in high regard in criminal court proceedings due to their ability to discover irrefutable facts that would otherwise go unnoticed. Nevertheless, forensic scientists can fall victim to natural logical fallacies. More specifically, confirmation bias is “a proclivity to search for or interpret additional information to confirm beliefs and to steer clear of information that may disagree with those prior beliefs” (Budlowe et al., 2009, p. 803). To restore the integrity of the forensic sciences, the sources of confirmation bias need to be identified and eliminated. Accordingly, empirical studies have given …