Biological and Chemical Physics Commons^™

Development Of Microfluidic Platforms For Studies Of Cellular Organization In Escherichia Coli, Anna Dawn Jennings

Masters Theses

Traditionally, bacteria cells have been imaged on agarose pads allowing them to grow in steady conditions for only a few doubling times. To understand the cellular organization in bacteria, tools are needed that allow the observation of log-phase cells for many generations. In recent years, several microfluidic platforms have been designed that allow microscopic imaging of bacteria for over one hundred generations. One of the most promising approaches has been the so-called mother machine design where bacteria grow in small dead-end channels all connected to a large main channel, which is used to flow fresh nutrients to the cells and …