Biological and Chemical Physics Commons^™

Reconstructing Three-Dimensional Shape Envelopes From Time-Resolved Small-Angle X-Ray Scattering Data, Jessica Lamb, Lisa W. Kwok, Xiangyun Qiu, Kurt Andresen, Hye Yoon Park, Lois Pollack

Physics and Astronomy Faculty Publications

Modern computing power has made it possible to reconstruct low-resolution, three-dimensional shapes from solution small-angle X-ray scattering (SAXS) data on biomolecules without a priori knowledge of the structure. In conjunction with rapid mixing techniques, SAXS has been applied to time resolve conformational changes accompanying important biological processes, such as biomolecular folding. In response to the widespread interest in SAXS reconstructions, their value in conjunction with such time-resolved data has been examined. The group I intron from Tetrahymena thermophila and its P4–P6 subdomain are ideal model systems for investigation owing to extensive previous studies, including crystal structures. The goal of this …