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Substrate Specificity, Metal Binding Properties, And Spectroscopic Characterization Of The Dape-Encoded N-Succinyl-L,L-Diaminopimelic Acid Desuccinylase From Haemophilus Influenzae, David L. Bienvenue, Danuta M. Gilner, Ryan S. Davis, Brian Bennett, Richard C. Holz
Substrate Specificity, Metal Binding Properties, And Spectroscopic Characterization Of The Dape-Encoded N-Succinyl-L,L-Diaminopimelic Acid Desuccinylase From Haemophilus Influenzae, David L. Bienvenue, Danuta M. Gilner, Ryan S. Davis, Brian Bennett, Richard C. Holz
Physics Faculty Research and Publications
The catalytic and structural properties of divalent metal ion cofactor binding sites in the dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae were investigated. Co(II)-substituted DapE enzyme was 25% more active than the Zn(II)-loaded form of the enzyme. Interestingly, Mn(II) can activate DapE, but only to ∼20% of the Zn(II)-loaded enzyme. The order of the observed kcat values are Co(II) > Zn(II) > Cd(II) > Mn(II) >Ni(II) ∼ Cu(II) ∼ Mg(II). DapE was shown to only hydrolyze l,l-N-succinyl-diaminopimelic acid (l,l-SDAP) and was inactive toward d,l-, l,d-, and d,d-SDAP. DapE was also inactive toward several acetylated amino acids as …
Density Of Resonant States And A Manifestation Of Photonic Band Structure In Small Clusters Of Spherical Particles, Alexey Yamilov, Hui Cao
Density Of Resonant States And A Manifestation Of Photonic Band Structure In Small Clusters Of Spherical Particles, Alexey Yamilov, Hui Cao
Physics Faculty Research & Creative Works
We introduce a numerical recipe for calculating the density of the resonant states of the clusters of dielectric spheres. Using truncated multipole expansions (generalized multisphere Mie solution) we obtain the scattering matrix of the problem. By introducing an infinitesimal absorption in the spheres we express the dwell time of the electromagnetic wave in terms of the elements of the scattering matrix. Using the parameters in recent light localization experiments [Phys. Rev. Lett. 87, 153901 (2001)], we demonstrate that the density of the resonant states, related to the dwell time, shows the formation of the photonic band structure in small clusters …