Physics Commons^™

Investigation Of G Protein-Coupled Receptor Quaternary Structure Through Fluorescence Micro-Spectroscopy And Theoretical Modeling: Interdependence Between Receptor-Receptor And Receptor-Ligand Interactions, Joel David Paprocki

Theses and Dissertations

Proteins are of high interest in biophysics research due to the important roles they play within cells, such as sensing of chemical (ions and small molecules) and physical (e.g., light) stimuli, providing structure, transporting ions/molecules, signaling, and intercellular communication. The studies described in this dissertation focus on a particular type of membrane proteins known as G protein-coupled receptors (GPCR), which play a key role in cellular response to external stimuli. We used the sterile 2 α-factor mating pheromone receptor (Ste2), a prototypical class D GPCR present within Saccharomyces cerevisiae (baker’s yeast). Ste2 is responsible for initiating the second messenger signal …

Investigatin Actin-Myosin Mechanics To Model Heart Disease Using Fluorescence Microscopy And Optical Trapping, Justin Edward Reynolds

Honors Theses

Hypertrophic cardiomyopathy (HCM) is a hereditary disease in which the myocardium becomes hypertrophied, making it more difficult for the heart to pump blood. HCM is commonly caused by a mutation in the β-cardiac myosin II heavy chain. Myosin is a motor protein that facilitates muscle contraction by converting chemical energy from ATP hydrolysis into mechanical work and concomitantly moving along actin filaments. Optical tweezers have been used previously to analyze single myosin biophysical properties; however, myosin does not work as a single unit within the heart. Multiple myosin interacts to displace actin filaments and do not have the same properties …

High-Throughput Automated Multi-Target Super-Resolution Imaging, Farzin Farzam

Physics & Astronomy ETDs

Super-resolution microscopy techniques developed through the past few decades enable us to surpass the classical diffraction limit of light, and thus open new doors to investigate the formerly inaccessible world of nanometer-sized objects. Most importantly, by using super-resolution microscopy, one can visualize sub-cellular structures in the range of 10 to 200 nm. At this range, we can investigate exciting problems in biology and medicine by visualizing protein-protein interactions and spatiotemporal analysis of structures of interest on the surface or inside cells. These techniques (collectively known as nanoscopy) have a high impact on understanding and solving biological questions. This dissertation starts …

Two-Photon Excitation Of Cesium Alkali Metal Vapor 72D, 82D Kinetics And Spectroscopy, Ricardo C. Davila

Theses and Dissertations

Pulsed excitation on the two-photon Cs 6²S_½ → 7²D_3/2,5/2 transition results in time-resolved fluorescence at 697 nm and 672 nm. The rates for fine structure mixing between the 7²D_3/2,5/2 states have been measured for helium and argon rare gas collision partners. The mixing rates are very fast, 1.26 ± 0.05 x 10^-9 cm³/(atom sec) for He and 1.52 ± 0.05 x 10^-10 cm³ /(atom sec) for Ar, driven by the small energy splitting and large radial distribution for the valence electron. …

A Study In Cross-Beam Polarization Fluorescence Photoactivation Localization Microscopy On Dendra2-Hemagluttinin In Fixed Nih3t3 Cells, Matthew M. Valles

Electronic Theses and Dissertations

Fluorescence microscopy is popular for its noninvasive properties and its use in imaging multiple species, simultaneously. Furthermore, superresolution fluorescence microscopy (SRFLM) utilizes photoswitchable proteins to improve the lateral resolution of conventional fluorescence microscopy by an order of magnitude. There is little work conducted on the study of excitation laser polarizations and their effect on the number of localizations as well as the brightness of molecules. This thesis attempts to study the effect of excitation wavelength polarization on the number of localizations and the brightness of molecules by comparing two orientations of circularly-polarized, excitation lasers. The first type of orientation involves …

Thermal Property Measurement Of Thin Fibers By Complementary Methods, Troy Robert Munro

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thin fibers are prevalent in many engineering materials. Measuring how well heat transfers in such small fibers can be difficult to determine, and previous methods have led to erroneous results. This dissertation details three proposed methods to improve the measurement of the thermal conductivity, thermal diffusivity, and volumetric heat capacity of thin fibers. Of particular interest is natural and synthetic spider silks because previously published values of the natural silk thermal conductivity was similar to copper, an excellent thermal conductor.

The three methods developed are the improved transient electrothermal technique (which was redeveloped to include radiation and convection heat losses …

A Novel Setup For High-Pressure Raman Spectroscopy Under A Microscope, Thomas Andrew Oakeson

Electronic Theses and Dissertations

Functional properties of biological molecules and cells are affected by environmental parameters such as temperature and pressure. While Raman spectroscopy provides an intrinsic probe of molecular structural changes, the incorporation of a microscope enables studies of minuscule amounts of biological compounds with spatial resolution on a micron scale. We have developed a novel setup which combines a Raman microscope and a high pressure cell. A micro-capillary made out of fused silica simultaneously serves as the supporting body and the optical window of the pressure cell. The cell has been tested over the pressure range from 0.1 to 4 kbar. Raman …

Detection Of Vapor Phase Mercury Species By Laser Fluorescence Methods, Xiaomei Tong

Dissertations

Elemental and compound mercury are often both volatile and air stable. Several mercury species emissions have been identified in off-gases from industrial processes. The high toxicity of mercury species and the presence of mercury species in municipal waste and coal have prompted a demand for a cost-effective, accurate, and rugged technique for real-time, continuous detection of mercury species vapors. Real-time, continuous emission measurements are important for process control, monitoring, and remediation. At present, there is no commercial continuous emission monitoring (CEM) technique or instrumentation to reliably monitor volatile mercury species emissions from industrial stacks. Conventional measurement methods, such as cold …

Molecular Fluorescence At A Rough Surface: The Orientation Effects, William Lee Blacke

Dissertations and Theses

The interaction between an emitting molecular dipole and a conducting substrate with a periodic surface roughness is looked at with particular interest in the different orientations of the dipole with respect to the substrate surface. A previous dynamical, perturbative theory for the effects of perpendicular dipole is extended to treat a dipole oriented parallel to the surface of the substrate. The results are then applied to study the modified fluorescence characteristics of the emitting dipoles. Numerical results demonstrate that some fluorescence characteristics are extremely sensitive to the molecular orientation with the dipole oriented along the grating (x direction) exhibiting unique …

Vibrational Energy Transfer Within The B3Π(0+U) State Of 79Br2 Upon Collision With N2, O2, No, And Sf6, Gregory S. Williams

Theses and Dissertations

Vibrational transfer and electronic quenching in the lower vibrational levels of the ⁷⁹Br₂(B; v'≤3) were investigated using spectrally resolved, temporally resolved pulsed laser induced fluorescence techniques. Spectrally resolved emissions from collisionally populated Br₂(B) vibrational levels were observed for N₂, O₂, NO, and SF₆collision partners. The vibrational transfer was efficient in the nonpredissociative vibrational levels and is adequately described by the Montroll-Shuler model. An average fundamental vibrational transfer rate coefficient of k_v(l,0)=3.4(±0.6) x 10^-11 cm³/molec-sec predicts the vibrational transfer rates for the 0≤v'≤3 collisions with …

Infrared Fluorescence Studies Of Electronic-To-Vibrational Energy Transfer In A Br2:No System, Michael R. Hawks

Theses and Dissertations

Steady-state photolysis techniques were used to study electronic-to- vibrational energy transfer mechanisms from atomic bromine to nitric oxide. Molecular bromine was photodissociated by 488nm radiation to produce equal parts Br(²P_1/2) and Br(²P_3/2). Side fluorescence intensity from Br(²P_1/2) at 2.7 µm and from NO (v=1 and 2) around 5.3 µm measured as a function of bromine pressure and nitric oxide pressure. The branching ratio collisional transfer into the first and second states of NO was determined, and previously reported rates for quenching of NO by molecular bromine were verified.