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Full-Text Articles in Physics
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Within condensed matter, single fluorophores are sensitive probes of their chemical environments, but it is difficult to use their limited photon budget to image precisely their positions, 3D orientations, and rotational diffusion simultaneously. We demonstrate the polarized vortex point spread function (PSF) for measuring these parameters, including characterizing the anisotropy of a molecule’s wobble, simultaneously from a single image. Even when imaging dim emitters (∼500 photons detected), the polarized vortex PSF can obtain 12 nm localization precision, 4°–8° orientation precision, and 26° wobble precision. We use the vortex PSF to measure the emission anisotropy of fluorescent beads, the wobble dynamics …
Why Do Molecules Echo Atomic Periodicity?, Ray Hefferlin, Jonathan Sackett, Jeremy Tatum
Why Do Molecules Echo Atomic Periodicity?, Ray Hefferlin, Jonathan Sackett, Jeremy Tatum
Faculty Works
Franck–Condon factors are investigated for sequences of free
main-group diatomic molecules. Theory-based Condon loci
(parabolas) and Morse-potential loci are plotted on Deslandres
tables to verify if they, indeed, follow the largest Franck–Condon
factors. Then, the inclination angles of the Condon loci
are determined. Thus, entire band systems are quantified by
one variable, the angle. For all available isoelectronic sequences,
this angle increases from a central minimum toward
magic-number molecular boundaries. The theory for the Condon
locus gives the angle in terms of the ratio of the upperstate
to the lower-state force constants. It is concluded that
the periodicity is caused …