Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Physics
Simultaneous Separation And Detection Of Cations And Anions Ion A Microfluidic Device With Suppressed Electroosmotic Flow And A Single Injection Point, B. R. Reschke, J. Schiffbauer, Boyd F. Edwards, A. T. Timperman
Simultaneous Separation And Detection Of Cations And Anions Ion A Microfluidic Device With Suppressed Electroosmotic Flow And A Single Injection Point, B. R. Reschke, J. Schiffbauer, Boyd F. Edwards, A. T. Timperman
All Physics Faculty Publications
A rapid and simultaneous separation of cationic and anionic peptides and proteins in a glass microfluidic device that has been covalently modified with a neutral poly(ethylene glycol) (PEG) coating to minimize protein adsorption is presented. The features of the device allow samples that contain both anions and cations to be introduced from a central flow stream and separated in different channels with different outlets—all in the presence of low electroosmotic flow (EOF) imparted by the PEG coating. The analytes are electrophoretically extracted from a central hydrodynamic stream and electrophoretically separated in two different channels, in which pressure driven flow has …