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Enhancement Of Vitamin D Metabolites In The Eye Following Vitamin D3 Supplementation And Uv-B Irradiation, Yanping Lin, John L. Ubels, Mark P. Schotanus, Zhaohong Yin
Enhancement Of Vitamin D Metabolites In The Eye Following Vitamin D3 Supplementation And Uv-B Irradiation, Yanping Lin, John L. Ubels, Mark P. Schotanus, Zhaohong Yin
University Faculty Publications and Creative Works
This study was designed to measure vitamin D metabolites in the aqueous and vitreous humor and in tear fluid, and to determine if dietary vitamin D3 supplementation affects these levels. We also determined if the corneal epithelium can synthesize vitamin D following UV-B exposure. Methods: Rabbits were fed a control or vitamin D3 supplemented diet. Pilocarpine- stimulated tear fluid was collected and aqueous and vitreous humor were drawn from enucleated eyes. Plasma vitamin D was also measured. To test for epithelial vitamin D synthesis, a human corneal limbal epithelial cell line was irradiated with two doses of UV-B (10 and …