Open Access. Powered by Scholars. Published by Universities.®

Physics Commons

Open Access. Powered by Scholars. Published by Universities.®

Nanoscience and Nanotechnology

2013

Atomic force microscopy

Articles 1 - 1 of 1

Full-Text Articles in Physics

Ultrastable Atomic Force Microscopy For Biophysics, Allison Beth Churnside Jan 2013

Ultrastable Atomic Force Microscopy For Biophysics, Allison Beth Churnside

Physics Graduate Theses & Dissertations

Atomic force microscopy (AFM) is a multifunctional workhorse of nanoscience and molecular biophysics, but instrumental drift remains a critical issue that limits the precision and duration of experiments. We have significantly reduced the two most important types of drift: in position and in force. The first, position drift, is defined as uncontrolled motion between the tip and the sample, which occurs in all three dimensions. By scattering a laser off the apex of a commercial AFM tip, we locally measured and thereby actively controlled its three-dimensional position above a sample surface to <0.4Å(Δf = 0.01-10 Hz) in air at room ...