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Articles 61 - 90 of 205

Full-Text Articles in Oceanography and Atmospheric Sciences and Meteorology

Synchronicity Between Ice Retreat And Phytoplankton Bloom In Circum‐Antarctic Polynyas, Yun Li, Rubao Ji, Meibing Jin, Julienne Stroeve Feb 2016

Synchronicity Between Ice Retreat And Phytoplankton Bloom In Circum‐Antarctic Polynyas, Yun Li, Rubao Ji, Meibing Jin, Julienne Stroeve

Marine Science Faculty Publications

Phytoplankton in Antarctic coastal polynyas has a temporally short yet spatially variant growth window constrained by ice cover and day length. Using 18‐year satellite measurements (1997–2015) of sea ice and chlorophyll concentrations, we assessed the synchronicity between the spring phytoplankton bloom and light availability, taking into account the ice cover and the incident solar irradiance, for 50 circum‐Antarctic coastal polynyas. The synchronicity was strong (i.e., earlier ice‐adjusted light onset leads to earlier bloom and vice versa) in most of the western Antarctic polynyas but weak in a majority of the eastern Antarctic polynyas. The west‐east asymmetry is related to sea …


Siderophore-Based Microbial Adaptations To Iron Scarcity Across The Eastern Pacific Ocean, Rene M. Boiteau, Daniel R. Mende, Nicholas J. Hawco, Matthew R. Mcilvin, Jessica N. Fitzsimmons, Peter N. Sedwick Jan 2016

Siderophore-Based Microbial Adaptations To Iron Scarcity Across The Eastern Pacific Ocean, Rene M. Boiteau, Daniel R. Mende, Nicholas J. Hawco, Matthew R. Mcilvin, Jessica N. Fitzsimmons, Peter N. Sedwick

OES Faculty Publications

Nearly all iron dissolved in the ocean is complexed by strong organic ligands of unknown composition. The effect of ligand composition on microbial iron acquisition is poorly understood, but amendment experiments using model ligands show they can facilitate or impede iron uptake depending on their identity. Here we show that siderophores, organic compounds synthesized by microbes to facilitate iron uptake, are a dynamic component of the marine ligand pool in the eastern tropical Pacific Ocean. Siderophore concentrations in iron-deficient waters averaged 9 pM, up to fivefold higher than in iron-rich coastal and nutrient-depleted oligotrophic waters, and were dominated by amphibactins, …


Regional Ocean-Colour Chlorophyll Algorithms For The Red Sea, Robert J.W. Brewin, Dionysios E. Raitsos, Giorgio Dall'olmo, Nikolaos Zarokanellos, Thomas Jackson, Marie Fanny Racault, Emmanuel S. Boss, Shubha Sathyendranath, Burt H. Jones, Ibrahim Hoteit Aug 2015

Regional Ocean-Colour Chlorophyll Algorithms For The Red Sea, Robert J.W. Brewin, Dionysios E. Raitsos, Giorgio Dall'olmo, Nikolaos Zarokanellos, Thomas Jackson, Marie Fanny Racault, Emmanuel S. Boss, Shubha Sathyendranath, Burt H. Jones, Ibrahim Hoteit

Marine Sciences Faculty Scholarship

The Red Sea is a semi-enclosed tropical marine ecosystem that stretches from the Gulf of Suez and Gulf of Aqaba in the north, to the Gulf of Aden in the south. Despite its ecological and economic importance, its biological environment is relatively unexplored. Satellite ocean-colour estimates of chlorophyll concentration (an index of phytoplankton biomass) offer an observational platform to monitor the health of the Red Sea. However, little is known about the optical properties of the region. In this paper, we investigate the optical properties of the Red Sea in the context of satellite ocean-colour estimates of chlorophyll concentration. Making …


Assessing Biogeochemical Impacts And Environmental Conditions Associated With Cross-Shelf High Chlorophyll Plumes In The Northern Gulf Of Mexico, Erin Brooke Jones Aug 2015

Assessing Biogeochemical Impacts And Environmental Conditions Associated With Cross-Shelf High Chlorophyll Plumes In The Northern Gulf Of Mexico, Erin Brooke Jones

Dissertations

The northern Gulf of Mexico is a complex marine system subject to episodic physical phenomena such as loop current eddies. Flow fields generated by these eddies can result in cross-shelf exchanges between riverine influenced shelf waters and the offshore water column. This study considers the impacts of high chlorophyll plumes (HCPs) resulting from cross-shelf exchanges to the bio-optical properties of affected waters and how these plumes are influenced by their environment. The seasonal, interannual and decadal chlorophyll cycles of the Gulf of Mexico and the northern Gulf of Mexico are described to provide context for evaluating the ecological effects of …


Exploration Of Spatial And Temporal Changes In Trophic Status Of Lakes In The Northern Temporal Forest Biome Using Remote Sensing, Aleksey Paltsev Apr 2015

Exploration Of Spatial And Temporal Changes In Trophic Status Of Lakes In The Northern Temporal Forest Biome Using Remote Sensing, Aleksey Paltsev

Electronic Thesis and Dissertation Repository

There is a critical need for detailed surveys of lakes covering large spatial (>100 km2) and temporal scales (decades) to determine if there is an increase in the magnitude and frequency of phytoplankton blooms. Remote sensing was used to: (1) develop a regression model that relates chlorophyll a (chl-a) as a proxy of lake phytoplankton biomass to Landsat TM and ETM+ optical reflectance (r2=0.85, p


Genetic Indicators Of Iron Limitation In Wild Populations Of Thalassiosira Oceanica From The Northeast Pacific Ocean, P. Dreux Chappell, Leann P. Whitney, Joselynn R. Wallace, Adam I. Darer, Samua Jean-Charles, Bethany D. Jenkins Jan 2015

Genetic Indicators Of Iron Limitation In Wild Populations Of Thalassiosira Oceanica From The Northeast Pacific Ocean, P. Dreux Chappell, Leann P. Whitney, Joselynn R. Wallace, Adam I. Darer, Samua Jean-Charles, Bethany D. Jenkins

OES Faculty Publications

Assessing the iron (Fe) nutritional status of natural diatom populations has proven challenging as physiological and molecular responses can differ in diatoms of the same genus. We evaluated expression of genes encoding flavodoxin (FLDA1) and an Fe-starvation induced protein (ISIP3) as indicators of Fe limitation in the marine diatom Thalassiosira oceanica. The specificity of the response to Fe limitation was tested in cultures grown under Fe-and macronutrient-deficient conditions, as well as throughout the diurnal light cycle. Both genes showed a robust and specific response to Fe limitation in laboratory cultures and were detected in small volume samples collected from …


Iron Supply And Demand In Antarctic Shelf Ecosystem, D. J. Mcgillicuddy Jr., Peter N. Sedwick, Michael S. Dinniman, K. R. Arrigo, T. S. Bibby, B. J. W. Greenan, Eileen E. Hofmann, John M. Klinck, W. O. Smith Jr., S. L. Mack, C. M. Marsay, B. M. Sohst, G. L. Van Dijken Jan 2015

Iron Supply And Demand In Antarctic Shelf Ecosystem, D. J. Mcgillicuddy Jr., Peter N. Sedwick, Michael S. Dinniman, K. R. Arrigo, T. S. Bibby, B. J. W. Greenan, Eileen E. Hofmann, John M. Klinck, W. O. Smith Jr., S. L. Mack, C. M. Marsay, B. M. Sohst, G. L. Van Dijken

OES Faculty Publications

The Ross Sea sustains a rich ecosystem and is the most productive sector of the Southern Ocean. Most of this production occurs within a polynya during the November-February period, when the availability of dissolved iron (dFe) is thought to exert the major control on phytoplankton growth. Here we combine new data on the distribution of dFe, high-resolution model simulations of ice melt and regional circulation, and satellite-based estimates of primary production to quantify iron supply and demand over the Ross Sea continental shelf. Our analysis suggests that the largest sources of dFe to the euphotic zone are wintertime mixing and …


The Influence Of Sea Ice And Snow Cover And Nutrient Availability On The Formation Of Massive Under-Ice Phytoplankton Blooms In The Chukchi Sea, Jinlun Zhang, Carin Ashjian, Robert Campbell, Yvette H. Spitz, Michael Steele, Victoria Hill Jan 2015

The Influence Of Sea Ice And Snow Cover And Nutrient Availability On The Formation Of Massive Under-Ice Phytoplankton Blooms In The Chukchi Sea, Jinlun Zhang, Carin Ashjian, Robert Campbell, Yvette H. Spitz, Michael Steele, Victoria Hill

OES Faculty Publications

A coupled biophysical model is used to examine the impact of changes in sea ice and snow cover and nutrient availability on the formation of massive under-ice phytoplankton blooms (MUPBs) in the Chukchi Sea of the Arctic Ocean over the period 1988–2013. The model is able to reproduce the basic features of the ICESCAPE (Impacts of Climate on EcoSystems and Chemistry of the Arctic Pacific Environment) observed MUPB during July 2011. The simulated MUPBs occur every year during 1988–2013, mainly in between mid-June and mid-July. While the simulated under-ice blooms of moderate magnitude are widespread in the Chukchi Sea, MUPBs …


How The Presence Of Plastic In The North Pacific Gyre Affects The Growth Of Thalassiosira Through Remote Sensing And Laboratory Replication, Jordynn Brennan, Hesham El-Askary Dec 2014

How The Presence Of Plastic In The North Pacific Gyre Affects The Growth Of Thalassiosira Through Remote Sensing And Laboratory Replication, Jordynn Brennan, Hesham El-Askary

Student Scholar Symposium Abstracts and Posters

Through the use of remote sensing, we are able to determine the approximate location of the garbage patch in the North Pacific Gyre. Though remote sensing does not penetrate the surface of the ocean, monthly satellite images can be analyzed to determine the rate of growth or rate of decrease of certain parameters, such as atmospheric gases, phytoplankton, and dissolved organic matter. Over the past decade, data from the Goddard Earth Sciences Data and Information Services Center (Giovanni program) has shown a significant increase in dissolved organic matter and chlorophyll a content in the area of the North Pacific Garbage …


Effects Of Selective Grazing By Microzooplankton On Phytoplankton Composition In A Subtropical Estuary, Amanda Mcgehee Sep 2014

Effects Of Selective Grazing By Microzooplankton On Phytoplankton Composition In A Subtropical Estuary, Amanda Mcgehee

Amanda McGehee

Rates of phytoplankton growth and microzooplankton grazing were measured at two locations within the Bay of St. Louis, MS, over the course of three months to explore the dynamics of the phytoplankton community. Community growth rates were estimated based on the changes in chlorophyll a (chl a) concentration using the dilution technique, (Landry and Hassett, 1982) and a C-specific rate was obtained using chl a labeling (Redalje and Laws, 1981). Concentrations of chl a were determined using high performance liquid chromatography (HPLC) and fluorometry. HPLC was also employed to obtain class specific growth and grazing rates, using either marker pigments …


Effects Of Selective Grazing By Microzooplankton On Phytoplankton Composition In A Subtropical Estuary, Amanda Marie Mcgehee Aug 2014

Effects Of Selective Grazing By Microzooplankton On Phytoplankton Composition In A Subtropical Estuary, Amanda Marie Mcgehee

Master's Theses

Rates of phytoplankton growth and microzooplankton grazing were measured at two locations within the Bay of St. Louis, MS, over the course of three months to explore the dynamics of the phytoplankton community. Community growth rates were estimated based on the changes in chlorophyll a (chl a) concentration using the dilution technique, (Landry and Hassett, 1982) and a C-specific rate was obtained using chl a labeling (Redalje and Laws, 1981). Concentrations of chl a were determined using high performance liquid chromatography (HPLC) and fluorometry. HPLC was also employed to obtain class specific growth and grazing rates, using either marker pigments …


Phytoplankton In Virginia Lakes And Reservoirs: Part Ii, Harold G. Marshall Apr 2014

Phytoplankton In Virginia Lakes And Reservoirs: Part Ii, Harold G. Marshall

Virginia Journal of Science

Phytoplankton composition from 16 Virginia lakes and reservoirs are discussed with emphasis on the dominant taxa, algal bloom producers, and potentially harmful species at these locations. This is a companion study to the more comprehensive publication regarding Virginia freshwater phytoplankton by Marshall (2013).


Phytoplankton Response To South Louisiana Crude Oil Exposure: Determining Impacts At Individual, Community, Toxin Production, Enzymatic-Activity And Gene-Expression Levels, Koray Ozhan Jan 2014

Phytoplankton Response To South Louisiana Crude Oil Exposure: Determining Impacts At Individual, Community, Toxin Production, Enzymatic-Activity And Gene-Expression Levels, Koray Ozhan

LSU Doctoral Dissertations

The Deepwater Horizon oil spill in the Gulf of Mexico (GoM) raised an important question. What is the ecological impact of the oil, the dispersant, and the dispersed oil to the GoM ecosystem? Significant and varying research efforts have contributed answers to this question. However, to better understand the complete ecological consequences of the spill in the GoM, the impact of the spill at the base of marine food web should be examined. This research aims to understand impact of the spilled oil, South Louisiana crude oil (LSC), the chemical dispersant, Corexit® EC9500A, and the dispersed oil on phytoplankton communities …


Phytoplankton And Nutrient Dynamics In A Tidally Dominated Eutrophic Estuary: Daily Variability And Controls On Bloom Formation, Ryan E. Morse, Margaret R. Mulholland, Todd A. Egerton, Harold G. Marshall Jan 2014

Phytoplankton And Nutrient Dynamics In A Tidally Dominated Eutrophic Estuary: Daily Variability And Controls On Bloom Formation, Ryan E. Morse, Margaret R. Mulholland, Todd A. Egerton, Harold G. Marshall

OES Faculty Publications

To better understand nutrient dynamics and factors that promote the initiation of algal blooms, the Lafayette River, a tidal subestuary of Chesapeake Bay that experiences seasonal algal blooms, was sampled daily for a period of 54 d in the fall of 2005. Three phytoplankton blooms (chl a concentrations exceeding twice the average of monthly measurements from 2000 to 2009) occurred during this period: a mixed bloom of Akashiwo sanguinea and Gymnodinium sp., a monospecific Skeletonema costatum bloom, and a monospecific Gymnodinium sp. bloom. Over the sampling period, nutrient concentrations increased following precipitation events and were elevated between bloom periods but …


The Great 2012 Arctic Ocean Summer Cyclone Enhanced Biological Productivity On The Shelves, Jinlun Zhang, Carin Ashjian, Robert Campbell, Victoria Hill, Yvette H. Spitz, Michael Steele Jan 2014

The Great 2012 Arctic Ocean Summer Cyclone Enhanced Biological Productivity On The Shelves, Jinlun Zhang, Carin Ashjian, Robert Campbell, Victoria Hill, Yvette H. Spitz, Michael Steele

OES Faculty Publications

A coupled biophysical model is used to examine the impact of the great Arctic cyclone of early August 2012 on the marine planktonic ecosystem in the Pacific sector of the Arctic Ocean (PSA). Model results indicate that the cyclone influences the marine planktonic ecosystem by enhancing productivity on the shelves of the Chukchi, East Siberian, and Laptev seas during the storm. Although the cyclone's passage in the PSA lasted only a few days, the simulated biological effects on the shelves last 1 month or longer. At some locations on the shelves, primary productivity (PP) increases by up to 90% and …


Judy Reservoir Monitoring Project 2013 Final Report, Robin A. Matthews, Joan Vandersypen Dec 2013

Judy Reservoir Monitoring Project 2013 Final Report, Robin A. Matthews, Joan Vandersypen

Judy Reservoir

The purpose of this study was to identify and count the phytoplankton and measure chlorophyll, total nitrogen, and total phosphorus levels in water samples collected from Judy Reservoir. Water quality and algal data have been collected on a weekly basis since October 2006; annual reports have been sent to the Skagit Public Utility District No. 1 in 2007, 2008, 2010 (January and December), 2011, and 2012. This project ended in 2013; the final chemistry sample was collected May 2, 2013 and the final algae sample was collected November 6, 2013.


Reservoir Management Techniques To Enhance Biological Productivity And Protect Water Quality, Ben Alan Thompson Dec 2013

Reservoir Management Techniques To Enhance Biological Productivity And Protect Water Quality, Ben Alan Thompson

Graduate Theses and Dissertations

Three reservoirs of similar size, watershed land use, and qualitative characteristics in northwest Arkansas, USA were selected to compare the effects of chemical fertilization and pulsed artificial-upwelling on whole-lake productivity, specifically primary production and phytoplankton biomass. Numerous water quality parameters were quantified over a two year period (2011-13) with the goal of understanding how each management technique would stimulate productivity. This experiment was the first step towards a larger goal to ultimately enhance sport fish production. The first year of monitoring occurred in 2011 and served as a control year for the three lakes. Treatments were initiated in two of …


Case Studies Of Tropical Cyclones And Phytoplankton Blooms Over Atlantic And Pacific Regions, Ashley Merritt-Takeuchi, Sen Chiao Sep 2013

Case Studies Of Tropical Cyclones And Phytoplankton Blooms Over Atlantic And Pacific Regions, Ashley Merritt-Takeuchi, Sen Chiao

Faculty Publications, Meteorology and Climate Science

This study investigates phytoplankton blooms following the passage of tropical cyclones in the Atlantic and Pacific Ocean basins. The variables of sea surface temperature (SST), chlorophyll (Chl-a), precipitation, and storm surface winds were monitored for two case studies, Typhoon Xangsane (2006) and Hurricane Earl (2010). Strong near-surface wind from tropical cyclones creates internal friction, which causes deep nutrient enriched waters to displace from the bottom of the ocean floor up toward the surface. In return, the abundance of upwelled nutrients near the surface provides an ideal environment for the growth of biological substances such as chlorophyll and phytoplankton. The inverse …


Regional To Global Assessments Of Phytoplankton Dynamics From The Seawifs Mission, D. A. Siegel, M. J. Behrenfeld, S. Maritorena, C. R. Mcclain, D. Antoine, S. W. Bailey, P. S. Bontempi, E. S. Boss, H. M. Dierssen, S. C. Doney, R. E. Eplee, R. H. Evans, G. C. Feldman, E. Fields, B. A. Franz, N. A. Kuring, C. Mengelt, N. B. Nelson, F. S. Patt, W. D. Robinson, J. L. Sarmiento, C. M. Swan, P. J. Werdell, T. K. Westberry, J. G. Wilding, J. A. Yoder Aug 2013

Regional To Global Assessments Of Phytoplankton Dynamics From The Seawifs Mission, D. A. Siegel, M. J. Behrenfeld, S. Maritorena, C. R. Mcclain, D. Antoine, S. W. Bailey, P. S. Bontempi, E. S. Boss, H. M. Dierssen, S. C. Doney, R. E. Eplee, R. H. Evans, G. C. Feldman, E. Fields, B. A. Franz, N. A. Kuring, C. Mengelt, N. B. Nelson, F. S. Patt, W. D. Robinson, J. L. Sarmiento, C. M. Swan, P. J. Werdell, T. K. Westberry, J. G. Wilding, J. A. Yoder

Marine Sciences Faculty Scholarship

Photosynthetic production of organic matter by microscopic oceanic phytoplankton fuels ocean ecosystems and contributes roughly half of the Earth's net primary production. For 13. years, the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) mission provided the first consistent, synoptic observations of global ocean ecosystems. Changes in the surface chlorophyll concentration, the primary biological property retrieved from SeaWiFS, have traditionally been used as a metric for phytoplankton abundance and its distribution largely reflects patterns in vertical nutrient transport. On regional to global scales, chlorophyll concentrations covary with sea surface temperature (SST) because SST changes reflect light and nutrient conditions. However, the ocean may …


Phytoplankton Life History Events: Resting Stages And Physiological Cell Death, Christine R. Kozik Aug 2013

Phytoplankton Life History Events: Resting Stages And Physiological Cell Death, Christine R. Kozik

Theses and Dissertations

Understanding and predicting changes in phytoplankton populations requires knowledge of key life history processes such as recruitment from benthic resting stages and losses due to sedimentation and cell death. Currently, these processes are poorly understood in freshwater systems. Phytoplankton resting stage and cell death life history events were separately examined in two freshwater systems in Wisconsin, four northern lakes and an urban pond. In the norther lakes, sedimentation and benthic recruitment were examined using sediment and recruitment traps that were sampled weekly over two summers. Sedimentation and benthic recruitment contributed little to changes in standing crop chl a, but rather …


Springtime Contribution Of Dinitrogen Fixation To Primary Production Across The Mediterranean Sea, E. Rahav, B. Herut, A. Levi, Margaret Mulholland, I. Berman-Frank May 2013

Springtime Contribution Of Dinitrogen Fixation To Primary Production Across The Mediterranean Sea, E. Rahav, B. Herut, A. Levi, Margaret Mulholland, I. Berman-Frank

OES Faculty Publications

Dinitrogen (N-2) fixation rates were measured during early spring across the different provinces of Mediterranean Sea surface waters. N-2 fixation rates, measured using N-15(2) enriched seawater, were lowest in the eastern basin and increased westward with a maximum at the Strait of Gibraltar (0.10 to 2.35 nmol NL-1 d(-1), respectively). These rates were 3-7 fold higher than N-2 fixation rates measured previously in the Mediterranean Sea during summertime and we estimated that methodological differences alone did not account for the seasonal changes we observed. Higher contribution of N-2 fixation to primary production (4-8 %) was measured in the western basin …


In Situ Measurements Of Phytoplankton Fluorescence Using Low Cost Electronics, Thomas Leeuw, Emmanuel S. Boss, Dana L. Wright Jan 2013

In Situ Measurements Of Phytoplankton Fluorescence Using Low Cost Electronics, Thomas Leeuw, Emmanuel S. Boss, Dana L. Wright

Marine Sciences Faculty Scholarship

Chlorophyll a fluorometry has long been used as a method to study phytoplankton in the ocean. In situ fluorometry is used frequently in oceanography to provide depth-resolved estimates of phytoplankton biomass. However, the high price of commercially manufactured in situ fluorometers has made them unavailable to some individuals and institutions. Presented here is an investigation into building an in situ fluorometer using low cost electronics. The goal was to construct an easily reproducible in situ fluorometer from simple and widely available electronic components. The simplicity and modest cost of the sensor makes it valuable to students and professionals alike. Open …


Phytoplankton In Virginia Lakes And Reservoirs, Harold G. Marshall Jan 2013

Phytoplankton In Virginia Lakes And Reservoirs, Harold G. Marshall

Biological Sciences Faculty Publications

This study involves a phytoplankton summer/autumn survey in 46 Virginia lakes and reservoirs during 2010-2012. A total of 307 taxa were identified which included several filamentous and colonial cyanabacteria in bloom concentrations. With the exception of one natural lake, the other sites sampled represent impoundments created decades ago, with the majority presently classified as meso- or eutrophic. Among the cyanobacteria were 6 known toxin producers (Anabaena circinalis, Anabaena spiroides, Aphanizomenon flos-aquae, Cylindrospermopsis raciborskii, Limnothrix redekei, and Microcystis aeruginosa). The study characterizes phytoplankton populations in these aging freshwater habitats taken from a large number …


Seasonal Dynamics Of Mesodinium Rubrum In Chesapeake Bay, Matthew D. Johnson, Doane K. Stoecker, Harold G. Marshall Jan 2013

Seasonal Dynamics Of Mesodinium Rubrum In Chesapeake Bay, Matthew D. Johnson, Doane K. Stoecker, Harold G. Marshall

Biological Sciences Faculty Publications

The photosynthetic ciliate Mesodinium rubrum is a common member of coastal phytoplankton communities that is well adapted to low-light, turbid ecosystems. It supports the growth of, or competes with, harmful dinoflagellate species for cryptophyte prey, as well as being a trophic link to copepods and larval fish. We have compiled data from various sources (n = 1063), on the abundance and distribution of M. rubrum in Chesapeake Bay and its tributaries. Because M. rubrum relies on obtaining organelles from cryptophyte algae to maintain rapid growth, we also enumerated cryptophyte algae in the portion of these samples that we collected …


A Preliminary Examination Of An In Situ Dual Dye Approach To Measuring Light Fluxes In Lotic Systems, Elizabeth C. Minor, Elizabeth James, Jay A. Austin, Veronica Nelson, Ryan Lusk, Kenneth Mopper Jan 2013

A Preliminary Examination Of An In Situ Dual Dye Approach To Measuring Light Fluxes In Lotic Systems, Elizabeth C. Minor, Elizabeth James, Jay A. Austin, Veronica Nelson, Ryan Lusk, Kenneth Mopper

Chemistry & Biochemistry Faculty Publications

Light is a critical parameter in aquatic ecosystems, affecting primary production and in situ photochemistry. However, measuring light exposure for suspended particles or dissolved components in a dynamic water column can be challenging with existing Eulerian approaches. Here, we assess the simultaneous deployment of two dyes differing in photolability (rhodamine WT and fluorescein) as a Lagrangian measure of sunlight exposure in a lotic system. Fluorescein is sensitive to light exposure; rhodamine WT is relatively photostable. We examined dye fluorescence at various pH, salinity, and temperature conditions. We also tested dye photolability as a function of pH and wavelength range. In …


Distributions Of Particulate Heme B In The Atlantic And Southern Oceans- Implications For Electron Transport In Phytoplankton, Martha Gledhill, Eric P. Achterberg, David J. Honey, Maria C. Nielsdottir, Micha J.A. Rijkenberg Jan 2013

Distributions Of Particulate Heme B In The Atlantic And Southern Oceans- Implications For Electron Transport In Phytoplankton, Martha Gledhill, Eric P. Achterberg, David J. Honey, Maria C. Nielsdottir, Micha J.A. Rijkenberg

OES Faculty Publications

Concentrations of heme b, the iron-containing component of b-type hemoproteins, ranged from b concentrations were enhanced in the photic zone and decreased with depth. Heme b concentrations correlated positively with chlorophyll a (chl a) in the TNA (r=0.41, pb did not correlate with chl a in the IB or SS. In the IB and SS, stations with high-chlorophyll and low-nutrient (Fe and/or Si) concentrations exhibited low heme bconcentrations relative to particulate organic carbon (< 0.1 μmolmol-1, and high chl a:heme b ratios (> 500). High chl a:heme b ratios resulted from relative decreases in …


Diatom Proteomics Reveals Unique Acclimation Strategies To Mitigate Fe Limitation, Brook L. Nunn, Jessica F. Faux, Anna A. Hippman, Maria T. Maldonado, H. Rodger Harvey, David R. Goodlett, Philip W. Boyd, Robert F. Strzepek Jan 2013

Diatom Proteomics Reveals Unique Acclimation Strategies To Mitigate Fe Limitation, Brook L. Nunn, Jessica F. Faux, Anna A. Hippman, Maria T. Maldonado, H. Rodger Harvey, David R. Goodlett, Philip W. Boyd, Robert F. Strzepek

OES Faculty Publications

Phytoplankton growth rates are limited by the supply of iron (Fe) in approximately one third of the open ocean, with major implications for carbon dioxide sequestration and carbon (C) biogeochemistry. To date, understanding how alteration of Fe supply changes phytoplankton physiology has focused on traditional metrics such as growth rate, elemental composition, and biophysical measurements such as photosynthetic competence (Fv/Fm). Researchers have subsequently employed transcriptomics to probe relationships between changes in Fe supply and phytoplankton physiology. Recently, studies have investigated longer-term (i.e. following acclimation) responses of phytoplankton to various Fe conditions. In the present study, the coastal diatom, Thalassiosira pseudonana, …


Phytoplankton In Virginia Lakes And Reservoirs, Harold G. Marshall Jan 2013

Phytoplankton In Virginia Lakes And Reservoirs, Harold G. Marshall

Virginia Journal of Science

This study involves a phytoplankton summer/autumn survey in 46 Virginia lakes and reservoirs during 2010-2012. A total of 307 taxa were identified which included several filamentous and colonial cyanabacteria in bloom concentrations. With the exception of one natural lake, the other sites sampled represent impoundments created decades ago, with the majority presently classified as meso- or eutrophic. Among the cyanobacteria were 6 known toxin producers (Anabaena circinalis, Anabaena spiroides, Aphanizomenon flos-aquae, Cylindrospermopsis raciborskii, Limnothrix redekei, and Microcystis aeruginosa). The study characterizes phytoplankton populations in these aging freshwater habitats taken from a large number …


Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan Jan 2013

Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan

OES Faculty Publications

Dissolved inorganic phosphorus (DIP) concentrations in surface water of vast areas of the ocean are extremely low (<10 nM) and phosphorus (P) availability could limit primary productivity in these regions. We explore the use of oxygen isotopic signature of dissolved phosphate (δ18OPO4) to investigate biogeochemical cycling of P in the Sargasso Sea, Atlantic Ocean. Additional techniques for studying P dynamics including 33P-based DIP turnover time estimates and percent of cells expressing alkaline phosphatase (AP) activity as measured by enzyme-labeling fluorescence are also used. In surface waters, δ18OPO4 values were lower than equilibrium by 3–6%, indicative of dissolved organic phosphorous (DOP) remineralization by extracellular enzymes. An isotope mass balance model using a variety of possible combinations of …


Freshwater Phytoplankton Populations Detected Using High Pressure Liquid Chromatography (Hplc) Of Taxon-Specific Pigments, Lauren Jeanne Simmons Dec 2012

Freshwater Phytoplankton Populations Detected Using High Pressure Liquid Chromatography (Hplc) Of Taxon-Specific Pigments, Lauren Jeanne Simmons

Theses and Dissertations

Phytoplankton are key primary producers in aquatic ecosystems, and the principle food source for primary consumers. Individual phytoplankton species respond to different physical, chemical and biological parameters, so monitoring taxonomic composition of the phytoplankton community is a means to monitor changes in environmental conditions. Phytoplankton community changes have frequently been monitored by estimating biomass (using chlorophyll a, measured fluorometrically), and taxonomic data obtained from cell counts. While such methods are useful, they are time-consuming. I hypothesized that high pressure liquid chromatography (HPLC) methods, which have been frequently used in marine systems, would allow separation and identification of key pigments. …