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Full-Text Articles in Physical Chemistry
Total Internal Reflection: Applications In Nonlinear Microscopy And Fluorescence Anisotropy, Brandon Colon
Total Internal Reflection: Applications In Nonlinear Microscopy And Fluorescence Anisotropy, Brandon Colon
Doctoral Dissertations
As technology advances to harness new energies and to create new cures, the sophistication of analysis grows not only in depth but in efficiency. Total internal reflection (TIR) has been coupled to microscopy leveraging its unique optical phenomenon on a breadth of topics. In this dissertation, the work presented will show how TIR was applied in two different instrumental analyses to evaluate two unique and complex systems. The first project features TIR paired with the transient absorption microscopy (TAM), a nonlinear optical technique, to gauge solvent mixing and diffusion in microreactors. Microreactors gained acclaim for their ability to produce high …
Development Of Metal-Based Luminescent Probes For Applications In Biomolecular Structure And Dynamics, Ayesha Sharmin
Development Of Metal-Based Luminescent Probes For Applications In Biomolecular Structure And Dynamics, Ayesha Sharmin
Graduate Student Theses, Dissertations, & Professional Papers
New transition-metal complexes containing luminescent properties have been designed and synthesized for application as structural probes for biomolecules. Potential DNA intercalators, 2- amino-anthracene substituted triosmium clusters, were synthesized by the reactions of 2-aminoanthracene with [Os3(CO)10(CH3CN)2]. The products [Os3(CO)10(μ-η2-(N-C(1))-NH2C14H8)(μ- H)], [Os3(CO)10(μ-η2-(N-C(3))-NHC14H9)(μ-H)], [Os3(CO)9(μ-η2-(N-C(3))-NHC14H8)(μ-H)2] and [Os3(CO)9(μ3-η2-(N-C(3))-NHC10H9)(μ-H)] obtained from this reaction have been structurally characterized by spectroscopic, photophysical and electrochemical techniques. Solid state structures are also reported for the complexes [Os3(CO)10(μ-η2-(N-C(3))-NHC14H9)(μ-H)] and [Os3(CO)9(μ-η2-(N-C(3))-NHC14H8)(μ-H)2]. To investigate bio-macromolecular dynamics on the sub-microsecond-to-microsecond timescale, a series of ruthenium based mono-nuclear metalligand charge-transfer complexes (MLCs) of formula [XRu(CO)(L–L)(L′)2][PF6] (X = H, TFA, Cl; L–L = 2,2′-bipyridyl, 1,10-phenanthroline, 5-amino-1,10-phenanthroline …