Analytical Chemistry Commons^™

Utilizing Nanostructures And Nano-Mechanics For Sensitive Analyte Detection Via Surface Enhanced Raman Spectroscopy (Sers) And Micro-Cantilever Sensing Platforms, Ryan Andrew Wallace

Doctoral Dissertations

The purpose of this dissertation is to present the effective utilization of nano-structures and nano-mechanics in conjunction with surface enhanced Raman spectroscopy (SERS) and micro-cantilever (MC) mechanical sensors for sensitive analytical detection. One of the most important attributes an Analytical Chemist can possess is the ability to develop and efficiently use the tools provided to obtain precise and accurate information that can be effectively communicated. The following is a brief outline of the background concepts and studies that will be present herein.

A discussion of SERS will be presented in which the history and concepts behind the technique will be …

Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera

Honors Undergraduate Theses

In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single …

Further Development Of Raman Spectroscopy For Body Fluid Investigation : Forensic Identification, Limit Of Detection, And Donor Characterization, Claire K. Muro

Legacy Theses & Dissertations (2009 - 2024)

The challenges to forensic body fluid analysis have placed limitations on the type of information that investigators can acquire and how that information can be collected. In recent years, Raman spectroscopy has proven itself useful for characterizing body fluids. In 2008, a large-scale investigation was undertaken to explore the use of Raman spectroscopy as a means of identifying body fluids. This work resulted in multidimensional Raman spectroscopic signatures for the five main body fluids: semen, peripheral blood, saliva, vaginal fluid, and sweat. These studies were incredibly successful and created the foundation for years of continued research. Accordingly, the studies included …