Analytical Chemistry Commons^™

Micro Bca Analysis Of Proteins, Anusha Chennupati

All Capstone Projects

The main objective of this project was to develop and validate methods for detection and analysis of proteins using a newly introduced microdrop plate reader equipped with a 16 well microdrop reader (2 uL sample volume). We used commercially available BSA, egg albumin, trypsinogen, Pepsin, β-lactoblobulin and lysozyme as out test moieties. Protein drop size, time, BCA reagent quantity, was assessed at various levels using a microdrop micro-BCA protocol (Pierce Chemical Company) with the appropriate controls (PBS blank) the method validated using the appropriate statistical and method parameters (i.e %RSD, and regression analysis of the standard curves). The micro method …