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Full-Text Articles in Analytical Chemistry
Disease Biomarker Discovery And Fungal Metabolites Extraction Protocol Optimization Using Gcms Based Metabolomics, Chathuri Udeshika Gamlath Mohottige
Disease Biomarker Discovery And Fungal Metabolites Extraction Protocol Optimization Using Gcms Based Metabolomics, Chathuri Udeshika Gamlath Mohottige
Theses and Dissertations
Metabolomics is a powerful science that can be applied for the discovery of disease biomarkers, and investigation of altered metabolomes due to abiotic and biotic perturbations. This dissertation is focused on untargeted metabolomic applications to investigate fungal metabolite alterations associated with pathogenicity, fungal disease propagations, and symbiosis. This dissertation employs qualitative analysis of metabolite mixtures using HS-SPME coupled GC-MS and TMS derivatization followed by GC-MS analytical platforms. In the first study, we discovered a biomarker combination to diagnose fungal soft tissue disease in sweet potato at an early stage of disease propagation. We used an HS-SPME GC-MS untargeted metabolomics workflow …
Mass Spectrometry Based Metabolomics And Lipidomics., Fang Yuan
Mass Spectrometry Based Metabolomics And Lipidomics., Fang Yuan
Electronic Theses and Dissertations
Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain …
Mass Spectrometry Method Development For The Discovery And Characterization Of Secondary Metabolites, Zachary Kelley
Mass Spectrometry Method Development For The Discovery And Characterization Of Secondary Metabolites, Zachary Kelley
Theses and Dissertations--Chemistry
Secondary metabolites are organic compounds produced by an organism for reasons other than growth and development. In plants, secondary metabolites generally act as defense agents produced to deter predators and inhibit other competitive species. For humans, these compounds can often have a beneficial effect and are pursued and utilized as natural pharmaceuticals. The development of sensitive, high-throughput analytical screening methods for plant derived metabolites is crucial for natural pharmaceutical product discovery and plant metabolomic profiling. Here, metabolomic profiling methods were developed using a microfluidic capillary zone electrophoresis device and evaluated against traditional separation approaches. An alkaloid screening assay was constructed …