Analytical Chemistry Commons^™

Using Photoactive Vitamin Nanoparticles As Photodynamic Antimicrobial Chemotherapeutic (Pact) Agents To Treat Chronic Wounds, Rahul A. Khanke

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The objective of the proposed study is to develop new materials and methods to manage microbial growth in chronic wounds using phototherapy. Chronic wounds are considered as a worldwide health problem. The most common chronic wounds can be classified into three categories: venous ulcers, diabetic ulcers, and pressure ulcers. Venous ulcers, which usually occur in the legs, account for about 70% to 90% of chronic wounds and can become infected easily. Venous ulcers are sores that develop after veins in the legs have been damaged. These ulcers can penetrate deeply into the skin. Occasionally, if a venous ulcer persists for …

Labeling Of Folic Acid On Bovine Serum Albumin, Hari Krishna Kosuri

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Recent Clinical research focused mainly on the therapeutic safety which mainly involves the avoidance of toxicities. To prevent the toxicities, site targeted drugs are improved which exhibit their therapeutic action on the specific target or the diseased target without disturbing the healthy cells. Folic acid, a natural Vitamin (Vit B9) has shown promising results in site specificity mainly in the cancer cells where they are abundantly present.

Folic acid is of great importance because of the promising results of several clinical trials which demonstrate that Folic acid increases the potency and simultaneously reducing the toxicity of the cancer therapies. Various …

Total Protein Determination Using Micro Plate Assay, Julie Ruffatti

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One of the largest challenges that food manufactures are facing today is the management of food allergens. Allergenic protein in trace amounts, part per million concentrations, will trigger a reaction in some individuals. Food manufacturers need to prevent allergen cross-contamination by performing adequate sanitation after production of an allergenic containing food. Allergen detection kits are used to determine if sufficient protein was removed from the equipment surfaces during sanitation. One kit on the market is the 3M™ Clean- Trace™ Surface Protein (Allergen) swab tests. The test qualitatively detects the presence of protein based on the biuret reaction and will yield …

Purification And Analysis Of Folate-Dye Conjugate, Dilipkumar Sakhiya

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The folate receptor has achieved great importance for use in targeted drug delivery and in vitro and in vivo diagnostic assays. The folate receptor (FR) is over-expressed on several cancer cell types and activated macrophages, but exists in relatively low abundance on the surface of non-cancerous tissue and non inflammatory cells. This unique attribute is the hallmark of folate-targeted methodologies allowing the diagnostic agent and/or drug to be ferried to the specific disease sites while preventing “off target” effects in normal tissues. Protein toxins, chemotherapeutic agents, radioimaging agents, magnetic resonance imaging (MRI) contrast agents liposomes with entrapped drugs, radiotherapeutic agents, …

Degree Of Labeling Of Folate-Bsa With Rhodamine Dye, Prathibha Yazala

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Folic acid is a very important vitamin. It is needed by cells for various physiological functions. It plays an important role in growth and multiplication of cells and therefore is needed very much by cancerous cells. One of the ways folate reaches the cells is the folate receptor mediated endocytosis. Therefore folate binding proteins are abundant on cancerous cells. Clinical trials proved that the folate receptor targeted drug delivery is very promising to increase the drug exposure to malignant cells and to reduce the toxicity to healthy cells. It was recently discovered that folate receptor is also over expressed on …

Ruthenium Complexes As Dna Photonucleases In Treatment Of Malignant Skin Carcinoma, Devi Kalyan Karumanchi

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A variety of Ruthenium complexes possessing terpyridine complexes as ligands in their coordination sphere have been investigated extensively as DNA photo nucleases in vitro. This work has led to the realization that compounds of Ruthenium bind to DNA purines and that the interactions are of an unprecedented bridging variety. Owing to the molar absorptivity of these complexes in the visible region, direct cleavage of duplex DNA by these complexes requires irradiation in the range of 400-500 nm.

Although the observation of DNA photo cleavage in vitro makes these systems good candidates for further investigation, it does not provide any information …

Synthesis And Analysis Of N-Acetyltyrosine-N-Ethyl Amide From N-Acetyl Yyrosine Ethyl Ester, Ravinder Reddy Akkuluri

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Amino acids gets oxidized when they are interacted with oxygen and light. And it is always difficult for the researchers to find and isolate a particular amino acid from a protein, unless they completely know how it behaves when interacted with light. For this we are synthesizing amide form of the amino acid which mimics the structure of a protein, and we induce light and find out the exact behavior of the particular amino acid. Here, the aim is to know the tyrosine interaction with light, so we synthesize amide form of tyrosine which is N-Acetyl tyrosine ethyl amide from …

Synthesis And Characterization Of An Europium-Porphyrin Complex, Alejandro Blinder

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Photodynamic therapy (PDT) is a promising treatment that has continued to improve over the past thirty years when the first commercially approved photosensitizer (PS) was introduced. Although PDT has many successful applications, the terrifying number of new cancer cases reported each year makes scientists focus their efforts towards the development of new efficient PS for PDT. The biggest obstacle that prohibits PDT from becoming a more widely used therapy is the ineffective photosensitizers (PS) that are available on the market today. The purpose of this study was to evaluate the synthesized metal-porphyrin complex as a possible candidate as a PS. …