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Full-Text Articles in Analytical Chemistry
Simultaneous Determination Of Enantiomers Of Structurally Related Anticholinergic Analogs In Human Serum By Liquid Chromatography–Electrospray Ionization Mass Spectrometry With On-Line Sample Cleanup, Vladimı́R Čápka, Yan Xu
Simultaneous Determination Of Enantiomers Of Structurally Related Anticholinergic Analogs In Human Serum By Liquid Chromatography–Electrospray Ionization Mass Spectrometry With On-Line Sample Cleanup, Vladimı́R Čápka, Yan Xu
Chemistry Faculty Publications
Trihexyphenidyl, biperiden and procyclidine are anticholinergic drugs produced as racemates for the treatment of Parkinson’s disease. This paper describes a simple and sensitive LC–MS method for the simultaneous determination of these compounds in human serum. An on-line sample clean-up procedure was used, where serum samples were directly injected into a “restricted-access media” pre-column. After the exclusion of the serum proteins, the drug molecules were eluted to a β-cyclodextrin analytical column for chiral separation. The quantitation was done by electrospray ionization MS using diphenidol as an internal standard. The method developed has limits of detection of 1 ng/ml, at least two-orders-of-magnitude …
Determination Of Lysophosphatidic Acids By Capillary Electrophoresis With Indirect Ultraviolet Detection, Yi Lung Chen, Yan Xu
Determination Of Lysophosphatidic Acids By Capillary Electrophoresis With Indirect Ultraviolet Detection, Yi Lung Chen, Yan Xu
Chemistry Faculty Publications
Lysophosphatidic acid (LPA) is the simplest form of lysophospholipid. Molecular species of LPA have been identified as the potent components in the ovarian cancer activation factor. The elevated plasma LPAs may be used as potential biomarkers for the early detection of ovarian cancer. This paper is the first report on the quantitative analysis of molecular species of LPA using capillary electrophoresis. In this work, the separation of LPAs was achieved within 14 min in an adenosine monophosphate-borate–methanol–water solution, and the measurement was accomplished by indirect UV detection. With LPA (D) as internal standard, the method had linear calibration ranges for …