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Full-Text Articles in Analytical Chemistry
Elucidating Molecular Structure And Interactions Of Disease-Related Noncovalent Assemblies Through Ion Mobility Spectrometry - Mass Spectrometry, Amber Leann Hope Gray
Elucidating Molecular Structure And Interactions Of Disease-Related Noncovalent Assemblies Through Ion Mobility Spectrometry - Mass Spectrometry, Amber Leann Hope Gray
Doctoral Dissertations
Ion mobility spectrometry – mass spectrometry (IMS-MS) is a powerful gas-phase technique that is routinely employed in the investigations of amyloid oligomers and conformational studies due to its ability to separate isobaric and isomeric species with the same mass-to-charge ratio (m/z). The goal of this dissertation is to use IMS-MS as a primary platform to probe the conformational landscape of a macrocyclic protein, Cyclosporin A (CycA), and to characterize interactions of amyloidogenic assemblies. Through five independent studies presented within document, the limits of IMS-MS are pushed by employing conditions which mimic biological environments.
Chapter 2 focused on how …