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Full-Text Articles in Analytical Chemistry
Radial Heterogeneity And Surface Properties Of Columns Used In High Performance Liquid Chromatography, Jude A. Abia
Radial Heterogeneity And Surface Properties Of Columns Used In High Performance Liquid Chromatography, Jude A. Abia
Doctoral Dissertations
The radial heterogeneity of some columns used in high performance liquid chromatography (HPLC) was investigated using an on-column microelectrochemical amperometric detector. Such a detector allowed the recording of the elution profiles at different spatial positions throughout the column exit cross-section. From this, we obtain information about the radial distribution of the mobile phase velocity, column efficiency, and analyte concentration. In all cases, the results obtained show that the spatial distribution of the mobile phase velocity does not follow a piston-flow behavior but exhibits radial heterogeneity with differences not exceeding 5% between the center and wall regions of any column. The …